##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294647_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4740102 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.468068408654496 31.0 31.0 34.0 28.0 34.0 2 31.60203577897691 31.0 31.0 34.0 28.0 34.0 3 31.699809202418006 33.0 31.0 34.0 29.0 34.0 4 35.296911543253714 37.0 35.0 37.0 32.0 37.0 5 35.41287951187548 37.0 35.0 37.0 33.0 37.0 6 35.0440256348914 37.0 35.0 37.0 32.0 37.0 7 34.902722135515226 37.0 35.0 37.0 32.0 37.0 8 34.89276960706753 37.0 35.0 37.0 32.0 37.0 9 36.40883677186694 39.0 37.0 39.0 32.0 39.0 10 36.163876220385134 39.0 35.0 39.0 31.0 39.0 11 36.34780222028977 39.0 37.0 39.0 32.0 39.0 12 36.29269264669832 39.0 37.0 39.0 32.0 39.0 13 36.16771748793592 39.0 35.0 39.0 31.0 39.0 14 37.25589554823926 40.0 37.0 41.0 31.0 41.0 15 37.30944355205858 40.0 37.0 41.0 31.0 41.0 16 37.35236667902927 40.0 37.0 41.0 31.0 41.0 17 37.39237995300523 40.0 37.0 41.0 31.0 41.0 18 37.395839794164765 40.0 37.0 41.0 31.0 41.0 19 37.420733773239476 40.0 37.0 41.0 31.0 41.0 20 37.47284847456869 40.0 37.0 41.0 31.0 41.0 21 37.35249051602687 40.0 37.0 41.0 31.0 41.0 22 37.32513140012599 40.0 37.0 41.0 31.0 41.0 23 37.33258735782479 40.0 37.0 41.0 31.0 41.0 24 37.25013512367455 40.0 37.0 41.0 31.0 41.0 25 36.21913790040805 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4598249503358254 1101 2 0.45021018771954147 1101 3 0.3951797740918188 1101 4 0.3210918267517542 1101 5 0.40126712504019224 1101 6 0.376135897886698 1101 7 0.5287821959006891 1101 8 0.4840796077172058 1101 9 0.6141675801693935 1101 10 0.7738767181848871 1101 11 0.7070466003571667 1101 12 0.7698466940744098 1101 13 0.6831370700531494 1101 14 0.8748326376191855 1101 15 0.9132717175550269 1101 16 0.878646211280099 1101 17 0.87072286474897 1101 18 0.8622930105865052 1101 19 1.0183330952232978 1101 20 0.9229251893324744 1101 21 1.2331137163660841 1101 22 1.1608559690192166 1101 23 1.0631601682125265 1101 24 1.2456891955755225 1101 25 1.0337049125936204 1102 1 0.3416215450165936 1102 2 0.3661345946172112 1102 3 0.31556683838858035 1102 4 0.2805830464241197 1102 5 0.3325315396916153 1102 6 0.4294202626134762 1102 7 0.4291506472479014 1102 8 0.49241686407745533 1102 9 0.5424929883290233 1102 10 0.6853912801970665 1102 11 0.6628580042997072 1102 12 0.661129415085874 1102 13 0.6453997104414739 1102 14 0.8601107044042706 1102 15 0.8357368158021288 1102 16 0.7806933390309965 1102 17 0.7871397433909522 1102 18 0.7387114320659691 1102 19 0.8458749199648778 1102 20 0.833530218982645 1102 21 1.050694398854091 1102 22 1.0106382904753417 1102 23 0.9097771014472258 1102 24 1.0415727865099669 1102 25 0.9489856298943664 1103 1 0.25573804856675864 1103 2 0.23835983020756757 1103 3 0.19167485720681654 1103 4 0.1762113727395942 1103 5 0.2129219465663752 1103 6 0.2270664610341413 1103 7 0.2568758581944053 1103 8 0.35681070756099587 1103 9 0.21601754664845174 1103 10 0.46344558315789897 1103 11 0.4203947091369571 1103 12 0.43047330162366393 1103 13 0.529595163699824 1103 14 0.6831854400144408 1103 15 0.674629930117689 1103 16 0.6765838324008371 1103 17 0.6187789893471844 1103 18 0.640563030678571 1103 19 0.7087023328150508 1103 20 0.6231648715188811 1103 21 0.8877308045826737 1103 22 0.7317375075741097 1103 23 0.7832723287553165 1103 24 0.8792833826881434 1103 25 0.7707126852637813 1104 1 0.17769299970832364 1104 2 0.12567011474203227 1104 3 0.08954554337895004 1104 4 0.10604173629953806 1104 5 0.1605748854582103 1104 6 0.16986773817357914 1104 7 0.22218381480439575 1104 8 0.1957682378310821 1104 9 0.03841938829001634 1104 10 0.45995605505749637 1104 11 0.3756104482890805 1104 12 0.45433689210872075 1104 13 -0.10916201705066442 1104 14 0.35440030190786587 1104 15 0.4392781015477567 1104 16 0.4190417873110661 1104 17 0.4483751464968293 1104 18 0.484814823177409 1104 19 0.5647832307476861 1104 20 0.5141282345364147 1104 21 0.6651343535092451 1104 22 0.6439115297059388 1104 23 0.6432751145630959 1104 24 0.656216244419717 1104 25 0.5893241315643252 1105 1 -0.05954001497103789 1105 2 -0.019302467732707385 1105 3 -0.09046826301239363 1105 4 -0.058829982645377754 1105 5 -0.039381659770640454 1105 6 -0.07721609060625667 1105 7 -0.07213570691022397 1105 8 -0.2022716727871412 1105 9 0.10429006883441616 1105 10 0.11007132458807689 1105 11 0.13093160063384346 1105 12 -0.23902202456002186 1105 13 -0.5621028429803587 1105 14 -0.9628954534416465 1105 15 -0.3462864030503283 1105 16 -0.06279042229270715 1105 17 0.08418624431403998 1105 18 -0.683264713936417 1105 19 -0.609757968108326 1105 20 -0.6229352228976239 1105 21 0.023470538830991927 1105 22 0.15112486797144697 1105 23 0.12841103064484116 1105 24 0.2249445281807283 1105 25 0.03431459162464989 1106 1 -0.4679613388481627 1106 2 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1 0.16393097615874908 2207 2 0.18019455110935567 2207 3 0.17633595816837655 2207 4 0.1531638467319283 2207 5 0.14317265165775694 2207 6 0.22854270615685124 2207 7 0.24475243195799834 2207 8 0.23390767415233427 2207 9 0.32261404080482947 2207 10 0.44188786172250616 2207 11 0.41614458641709007 2207 12 0.38735954797722627 2207 13 0.46970103195566537 2207 14 0.4494592334243066 2207 15 0.4582954182231731 2207 16 0.4625328779068951 2207 17 0.5127057895033644 2207 18 0.5085663318795071 2207 19 0.5833123959171616 2207 20 0.5645874673326006 2207 21 0.7989220825785992 2207 22 0.7015486258114123 2207 23 0.6037513001035109 2207 24 0.8071353549534521 2207 25 0.6911379369947355 2208 1 0.10721239643127589 2208 2 0.13312526085911003 2208 3 0.1296371415976978 2208 4 0.09200447831836556 2208 5 0.07677109136712801 2208 6 0.19853138800422698 2208 7 0.17115656354545905 2208 8 0.13549994565665457 2208 9 0.22590377867108202 2208 10 0.33626173255806435 2208 11 -0.07135997556116536 2208 12 -0.26618526732379877 2208 13 -0.0043211954389832385 2208 14 -0.5048969341992802 2208 15 0.12705034789602365 2208 16 0.17810730066059222 2208 17 0.07477357733640133 2208 18 0.26983245143942725 2208 19 0.4165805133413727 2208 20 0.3393921231670447 2208 21 0.6429727797959544 2208 22 0.6403537705941531 2208 23 0.5896617350024158 2208 24 0.684775786924483 2208 25 0.6388347493733519 2209 1 0.04777243952023724 2209 2 0.05071716035236662 2209 3 0.10528190302365203 2209 4 -0.4409228174570856 2209 5 -0.02064315719743348 2209 6 0.14715833028634506 2209 7 0.18873166779292916 2209 8 0.22161906627319183 2209 9 0.3027355462782708 2209 10 0.2822400494415973 2209 11 0.34581528371344206 2209 12 0.31235990533087943 2209 13 0.42379715686488595 2209 14 0.4245341317005398 2209 15 -0.16820697064245138 2209 16 -0.17938252288527679 2209 17 0.22417073206355553 2209 18 -0.1429695087749252 2209 19 0.3045289532800979 2209 20 0.2617952518375404 2209 21 0.6341482608607691 2209 22 0.5938639140633342 2209 23 0.6053897993122774 2209 24 0.6955133903241446 2209 25 0.6079594714399619 2210 1 -0.17737061300367074 2210 2 -0.1663575798012431 2210 3 -0.06406653903766824 2210 4 -0.049190330564847784 2210 5 -0.02470388914843369 2210 6 -0.5735209808431918 2210 7 -0.17123459638368388 2210 8 -0.2910919299084185 2210 9 -0.06325535226505963 2210 10 0.12052811855279799 2210 11 0.12933925752241038 2210 12 0.06975670878295404 2210 13 0.1069587730843864 2210 14 0.1556665666667456 2210 15 0.038573520785902815 2210 16 0.17118188671602752 2210 17 -0.9377774457403234 2210 18 -1.4865490258808691 2210 19 -0.3629999636535146 2210 20 -0.12815750114030777 2210 21 -0.3134171789981508 2210 22 -0.27708360894543915 2210 23 -0.1579623258185947 2210 24 0.23053552799611765 2210 25 0.21075939497386997 2211 1 -0.2560481644264563 2211 2 -0.1989897519516859 2211 3 -0.14366403547398576 2211 4 -0.08153717528584536 2211 5 -0.041243643480576964 2211 6 -0.0500462357648388 2211 7 -0.13170464816352023 2211 8 -0.08514845980712238 2211 9 -0.09752338229498037 2211 10 0.010645322866430718 2211 11 -0.23926198189629133 2211 12 -0.20480769499431517 2211 13 -0.023851754070896902 2211 14 -0.07523095426773807 2211 15 -0.1651391039039538 2211 16 -0.20235181812891057 2211 17 -0.29107920385199293 2211 18 -0.393756667807331 2211 19 -0.193421114364412 2211 20 -0.2812772651706297 2211 21 0.0366820146644784 2211 22 -0.06606798419354476 2211 23 -0.6873456388202825 2211 24 -0.11302627258769604 2211 25 -0.5930678884920653 2212 1 -0.14541266932619834 2212 2 -0.2503168758694656 2212 3 -0.15541571407242571 2212 4 -0.050417535653799916 2212 5 -0.09478024689297371 2212 6 -0.08073823029799598 2212 7 -0.2161728746194882 2212 8 -0.31938106609292305 2212 9 -0.15862899152515553 2212 10 -0.4766322051671281 2212 11 -0.20129549162081162 2212 12 -0.26670957130928485 2212 13 -0.23383157673998767 2212 14 -0.2355811790617537 2212 15 -0.16719231867760698 2212 16 -0.2566560853038027 2212 17 -0.5240556374754561 2212 18 -0.38311427012718013 2212 19 -0.39216140564359137 2212 20 -0.5084824880743071 2212 21 -0.048980648748049305 2212 22 -0.37954111777833077 2212 23 -0.23002099644052265 2212 24 -0.3532743440432 2212 25 -0.1075521410431719 2213 1 -0.2011639308411901 2213 2 -0.20885667226681193 2213 3 -0.13753923986013916 2213 4 -0.0939376025434342 2213 5 -0.11541811874651842 2213 6 -0.2677919326978113 2213 7 -0.3941667724074307 2213 8 -0.3425515026357857 2213 9 -0.3274864403311213 2213 10 -0.2847715438985716 2213 11 -0.07590053474702074 2213 12 0.005580005056373238 2213 13 0.0067818715965728416 2213 14 -0.7124323789678755 2213 15 -0.31855211448819887 2213 16 -0.3675648895049406 2213 17 -0.3901725916501917 2213 18 -0.3809594264963607 2213 19 -0.4516895110240995 2213 20 -0.4321835676964412 2213 21 -0.14710194488135642 2213 22 -0.4523430012495453 2213 23 -0.6714272922804554 2213 24 -0.7564185968155996 2213 25 -0.5894361990141874 2214 1 -0.25888166128822476 2214 2 -0.1592158142745248 2214 3 -0.19338045089744327 2214 4 -0.08699070639750772 2214 5 -0.08488795120498338 2214 6 -0.020852527145095223 2214 7 -0.13337102752890928 2214 8 -0.12419538783422013 2214 9 -0.18811863091349323 2214 10 -0.06336925452553999 2214 11 0.08972877186018025 2214 12 -0.1719887651701626 2214 13 -0.23058863985416167 2214 14 -0.2017028300293333 2214 15 -0.06798499722461315 2214 16 -0.27719881821462167 2214 17 -0.4628190550062854 2214 18 -0.29756609099521114 2214 19 -0.5178426752674525 2214 20 -0.4243242514888905 2214 21 -0.5717428028736435 2214 22 -0.6351601223842493 2214 23 -1.0434196352296183 2214 24 -0.42108215885397016 2214 25 -0.9843023361133305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 25.0 9 56.0 10 105.0 11 293.0 12 652.0 13 1624.0 14 3254.0 15 5581.0 16 8291.0 17 11073.0 18 13153.0 19 14986.0 20 16570.0 21 18184.0 22 20030.0 23 22749.0 24 25978.0 25 29800.0 26 34324.0 27 39390.0 28 47256.0 29 59563.0 30 76760.0 31 101393.0 32 137174.0 33 187618.0 34 259835.0 35 364497.0 36 526719.0 37 838956.0 38 1874208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.47162664151751 21.266942048759145 12.23244270223025 25.02898860749309 2 14.579960515617596 21.81465715294734 39.97635493919751 23.62902739223755 3 17.658847728540696 27.048819300839522 30.178570410052213 25.113762560567565 4 11.697857309691786 17.624779275659144 39.18380574865456 31.493557665994516 5 12.758565680557279 38.39930888781413 35.50574083968274 13.33638459194586 6 30.296223542803613 39.58845826401336 17.411707365447164 12.703610827735867 7 25.666605051436058 33.82992677072044 23.490321344528738 17.013146833314767 8 24.129081129897724 37.7370816531939 21.193621694846588 16.940215522061784 9 24.63574546654369 16.5190464657648 22.319250343664216 36.5259577240273 10 14.327220244256681 30.25262111323417 34.0121465370635 21.40801210544565 11 32.335778596383825 25.937556073927865 23.322827498126472 18.403837831561837 12 20.716400982526046 27.474948982358114 32.39513192646371 19.413518108652124 13 26.68528090124681 23.66209573637687 27.374106031518323 22.278517330858 14 20.10020991339754 22.77444330756005 29.23013470322043 27.895212075821984 15 22.72675873062621 30.308976425927387 24.910707466902164 22.053557376544237 16 21.11807322249041 31.303911034807065 26.4688030909046 21.109212651797925 17 19.51581601987638 30.177513170083582 29.34466136677736 20.962009443262684 18 19.89721692460887 29.644162123846012 30.30959393090412 20.149027020641004 19 20.39133152590688 28.74035906198069 30.74604547229547 20.12226393981696 20 21.72283190343814 28.154860318082502 29.839026297174463 20.283281481304897 21 21.783630542503783 28.771379313836682 29.171603275545475 20.273386868114063 22 20.641969474329343 28.462228992108557 30.226658748150033 20.669142785412067 23 20.604106163295498 28.775695165538913 29.91442785733785 20.705770813827744 24 20.59730372833828 28.183745451837417 30.10381002430117 21.115140795523132 25 21.119587882899875 28.13378953471004 29.38828002967081 21.358342552719275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1470.0 1 1470.0 2 1909.5 3 2349.0 4 2349.0 5 2349.0 6 5876.0 7 9403.0 8 9403.0 9 9403.0 10 13822.5 11 18242.0 12 18242.0 13 18242.0 14 28447.0 15 38652.0 16 38652.0 17 38652.0 18 66195.0 19 93738.0 20 93738.0 21 93738.0 22 139950.5 23 186163.0 24 186163.0 25 186163.0 26 246904.0 27 307645.0 28 307645.0 29 307645.0 30 369572.0 31 431499.0 32 431499.0 33 431499.0 34 478697.0 35 525895.0 36 525895.0 37 525895.0 38 548727.5 39 571560.0 40 571560.0 41 571560.0 42 565222.0 43 558884.0 44 558884.0 45 558884.0 46 558960.0 47 559036.0 48 559036.0 49 559036.0 50 518642.0 51 478248.0 52 478248.0 53 478248.0 54 423992.5 55 369737.0 56 369737.0 57 369737.0 58 315922.0 59 262107.0 60 262107.0 61 262107.0 62 216628.5 63 171150.0 64 171150.0 65 171150.0 66 129895.0 67 88640.0 68 88640.0 69 88640.0 70 64655.5 71 40671.0 72 40671.0 73 40671.0 74 28524.0 75 16377.0 76 16377.0 77 16377.0 78 11100.0 79 5823.0 80 5823.0 81 5823.0 82 3935.0 83 2047.0 84 2047.0 85 2047.0 86 1295.0 87 543.0 88 543.0 89 543.0 90 366.5 91 190.0 92 190.0 93 190.0 94 108.0 95 26.0 96 26.0 97 26.0 98 16.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09748735364766413 2 0.0 3 0.13373129945304973 4 0.012510279314664538 5 0.022657740276475064 6 0.012805631608771286 7 0.021391944730303272 8 0.019240092301811226 9 2.109659243619652E-4 10 0.3991686254852743 11 0.06438680011527179 12 0.001582244432714739 13 4.219318487239304E-5 14 0.001097022806682219 15 0.0013501819159165774 16 2.109659243619652E-5 17 6.328977730858956E-5 18 4.641250335963235E-4 19 6.118011806496991E-4 20 2.3206251679816175E-4 21 1.2657955461717912E-4 22 0.0033121650124828535 23 1.6877273948957217E-4 24 2.109659243619652E-5 25 0.0018986933192576866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4740102.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.87997483802128 #Duplication Level Percentage of deduplicated Percentage of total 1 86.60714990061332 64.85141205350664 2 9.776639534280418 14.641490446546435 3 1.9347506689129261 4.346222442181342 4 0.6355948596804655 1.9037330840019566 5 0.3076360080207221 1.1517888269930492 6 0.16434056005470954 0.7383490201057784 7 0.10745888814093624 0.5632563188080534 8 0.0773420637802843 0.463309743183066 9 0.06019129530316757 0.4056410409992186 >10 0.2954780699527322 3.919235016972072 >50 0.018196616349127167 0.9519270149251977 >100 0.012537893011846548 1.7212120559356856 >500 0.0015536881380285173 0.8279396002790368 >1k 9.604606969785695E-4 1.6395055876265723 >5k 2.8248844028699407E-5 0.19589540753198637 >10k+ 1.4124422014349702E-4 1.679082340404011 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 25691 0.5419925562783248 No Hit GGTATCAACGCAGAGTACTTTTTTT 16426 0.34653262735696405 No Hit TATCAACGCAGAGTACTTTTTTTTT 15182 0.32028846636633557 No Hit CCCATGTACTCTGCGTTGATACCAC 11553 0.2437289324153784 No Hit GTACATGGGAAGCAGTGGTATCAAC 10527 0.22208382857584075 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9261 0.195375542551616 No Hit GAGTACATGGGAAGCAGTGGTATCA 4940 0.10421716663481081 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4931 0.10402729730288506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.109659243619652E-5 0.0 5 0.0 4.219318487239304E-5 0.0 2.109659243619652E-5 0.0 6 0.0 4.219318487239304E-5 0.0 2.109659243619652E-5 0.0 7 0.0 4.219318487239304E-5 0.0 6.328977730858956E-5 0.0 8 0.0 4.219318487239304E-5 0.0 8.438636974478609E-5 0.0 9 6.328977730858956E-5 4.219318487239304E-5 0.0 1.054829621809826E-4 0.0 10 6.328977730858956E-5 4.219318487239304E-5 0.0 1.054829621809826E-4 0.0 11 8.438636974478609E-5 4.219318487239304E-5 0.0 1.054829621809826E-4 0.0 12 8.438636974478609E-5 4.219318487239304E-5 0.0 1.2657955461717912E-4 0.0 13 8.438636974478609E-5 4.219318487239304E-5 0.0 1.4767614705337565E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGCC 120 1.3091994E-7 11.0636425 18 TAGACAG 735 0.0 10.232357 5 TAGCACG 95 1.6109238E-4 10.0210285 4 TATTCCG 200 2.3646862E-11 9.995976 5 CAGGACT 670 0.0 9.662066 4 ATCGTTC 120 1.66037E-5 9.519978 8 CACGACA 80 0.0044407058 9.519977 4 GCGTTCT 80 0.004559221 9.489629 11 AAATCGC 130 4.3407017E-6 9.483122 17 AGACCGT 125 2.6893065E-5 9.139178 6 TAGACTG 605 0.0 8.81188 5 TATACTG 920 0.0 8.79563 5 CCGTACA 315 0.0 8.7304945 17 CCAGGAC 765 0.0 8.692037 3 TACGCTG 110 7.001277E-4 8.654524 5 CCCCGTA 285 0.0 8.651178 15 GCGACTA 375 0.0 8.598031 16 TTAGACA 565 0.0 8.593253 4 GTCTAGG 410 0.0 8.5812025 1 TGTCCCG 310 0.0 8.571278 11 >>END_MODULE