FastQCFastQC Report
Wed 25 May 2016
SRR1294646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276442
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24410.8830062002155967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA21320.7712286844980141No Hit
GGTATCAACGCAGAGTACTTTTTTT16790.6073606760188394No Hit
TATCAACGCAGAGTACTTTTTTTTT14500.5245223229465855No Hit
GTACTGGTTCACTATCGGTCAGTCA12120.43842831407673216No Hit
ATACAGGGTGACAGCCCCGTACACA9880.3573986586698114No Hit
GGATACCACGTGTCCCGCCCTACTC8380.30313772870981975No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7200.2604524638079597No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6780.24525940341916208No Hit
GTTAATGATAGTGTGTCGAAACACA5400.1953393478559698No Hit
GTTCACTATCGGTCAGTCAGGAGTA4710.17037932007437365No Hit
GGTATCAACGCAGAGTACATGGGGG4670.1689323619421072No Hit
GTATTTAGCCTTGGAGGATGGTCCC4230.15301582248717632No Hit
GGTTAATGAGGCGAACCGGGGGAAC4060.14686625042504395No Hit
CCCATATTCAGACAGGATACCACGT4050.14650451089197736No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3990.14433407369357767No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3890.14071667836291157No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3880.14035493882984496No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3640.1316731900362463No Hit
GCCTTGGAGGATGGTCCCCCCATAT3620.13094971097011307No Hit
CACACACACTGATTCAGGCTCTGGG3430.12407665984184749No Hit
TCTAAGTACCCCGAGGAAAAGAAAT3340.12082100404424798No Hit
TCACGGTACTGGTTCACTATCGGTC3240.11720360871358187No Hit
AGCGTACACGGTGGATGCCCTGGCA3210.11611839011438205No Hit
CTATCGGTCAGTCAGGAGTATTTAG3170.1146714319821156No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3150.11394795291598236No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3120.11286273431678254No Hit
ATCATTAACTGAATCCATAGGTTAA3110.11250099478371595No Hit
GTGGATGCCCTGGCAGTCAGAGGCG3060.11069229711838288No Hit
GGGTTTCCCCATTCGGAAATCGCCG3030.10960707851918304No Hit
CATCTAAGTACCCCGAGGAAAAGAA2900.1049044645893171No Hit
CGGTACTGGTTCACTATCGGTCAGT2870.10381924599011727No Hit
GTATCAACGCAGAGTACATGGGGGT2860.10345750645705068No Hit
TATTCAGACAGGATACCACGTGTCC2840.10273402739091744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA250.00603207518.99331
GGTATCA3550.015.5156531
CGAATGG653.347579E-614.61552419
AGTATGT400.00523896114.26046917
GTACATA500.001498084413.2953091
CCTTGGG500.001500131113.2929022
CCGAATG655.3561824E-513.17067418
ACCGGGG1353.601599E-1011.97175215
CCGGGGG1302.5411282E-911.70089816
CGGGGGA1354.5929482E-911.26753117
ACAGGGT1553.092282E-1011.026373
GGGGAAC1502.0918378E-910.76676819
TGTCCCG1951.8189894E-1210.71222911
CAGGGTG1803.45608E-1110.5499234
GTACTGG3100.010.415681
GTATCAA7600.010.2463861
AACCGGG1605.980837E-910.10116614
CAGAAGG856.588701E-410.0534554
AGGGTGA1803.8926373E-1010.0224275
AGAGGCG951.6325741E-410.00009519
Produced by FastQC (version 0.11.5)