##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294646_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 276442 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.093697773854913 31.0 28.0 33.0 20.0 34.0 2 29.108974034336317 31.0 28.0 34.0 18.0 34.0 3 29.12594323583247 31.0 28.0 34.0 18.0 34.0 4 32.41701333371919 35.0 32.0 37.0 19.0 37.0 5 33.15131202928643 35.0 33.0 37.0 26.0 37.0 6 33.08562013008154 35.0 33.0 37.0 25.0 37.0 7 33.022518285933394 35.0 33.0 37.0 25.0 37.0 8 32.81667040464184 35.0 33.0 37.0 22.0 37.0 9 33.59572351524009 38.0 33.0 39.0 17.0 39.0 10 33.8402811439651 38.0 33.0 39.0 18.0 39.0 11 34.03164135695734 38.0 33.0 39.0 20.0 39.0 12 33.89783752107133 38.0 33.0 39.0 19.0 39.0 13 33.64817936493007 37.0 33.0 39.0 17.0 39.0 14 34.40557151228829 38.0 32.0 41.0 17.0 41.0 15 34.431168201648084 38.0 32.0 41.0 17.0 41.0 16 34.60918746066083 38.0 33.0 40.0 18.0 41.0 17 34.658275515297966 38.0 33.0 40.0 19.0 41.0 18 34.601373886746586 38.0 32.0 40.0 19.0 41.0 19 34.94641190557151 39.0 33.0 41.0 22.0 41.0 20 35.05952424016611 39.0 33.0 41.0 23.0 41.0 21 35.104846586264024 39.0 33.0 41.0 23.0 41.0 22 35.18346705638072 39.0 33.0 41.0 24.0 41.0 23 35.19473886023108 39.0 33.0 41.0 24.0 41.0 24 35.26092995999161 39.0 33.0 41.0 25.0 41.0 25 34.11332576091911 38.0 31.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2084403548995049 1101 2 1.2016357995947367 1101 3 1.3149433325158952 1101 4 1.238202054773609 1101 5 1.155239166015285 1101 6 0.8520287525296979 1101 7 0.4657701649068784 1101 8 0.9022541336622112 1101 9 1.0740714209012339 1101 10 1.580445920362493 1101 11 0.9372531291276616 1101 12 1.0430910019477722 1101 13 0.8666405644267243 1101 14 1.058598521821935 1101 15 1.1946824260769162 1101 16 0.8850822219557628 1101 17 0.9577210630439552 1101 18 1.4692003460194698 1101 19 1.2948326216664299 1101 20 1.0636045832724434 1101 21 0.7467003659084881 1101 22 0.7832824294880609 1101 23 0.790479287755538 1101 24 0.5962086153099975 1101 25 0.7748749898746823 1102 1 0.914577602865549 1102 2 0.8146605018704136 1102 3 1.1256967814016399 1102 4 1.1116692166648932 1102 5 0.5957090398509166 1102 6 0.6528334925367716 1102 7 0.4486878632915037 1102 8 0.439587584906171 1102 9 1.1527767622524863 1102 10 0.8741907617727023 1102 11 0.6496470095662943 1102 12 0.8260396518711275 1102 13 0.7661880824080924 1102 14 1.2627607668402163 1102 15 1.323816667322049 1102 16 1.4035204957442318 1102 17 1.3391619308661404 1102 18 1.2013295171077871 1102 19 0.6835102540211011 1102 20 0.013470459937927615 1102 21 0.2429935506744343 1102 22 0.5201427643542331 1102 23 0.6676827425368188 1102 24 0.5375259717538157 1102 25 0.7734232153197951 1103 1 0.5635864887165383 1103 2 0.3778155485780914 1103 3 0.8129831792143527 1103 4 0.6127229874555056 1103 5 0.4815315506734237 1103 6 0.5105744358040312 1103 7 0.4831774964653448 1103 8 0.26514579862227805 1103 9 0.11757512178768792 1103 10 0.3937852024197781 1103 11 0.5152057928882314 1103 12 0.2612897088317112 1103 13 0.1251394380699793 1103 14 0.1042964241653479 1103 15 0.412632456795734 1103 16 0.191370803331381 1103 17 0.4431149953455247 1103 18 0.8534854751847973 1103 19 0.31819355317808373 1103 20 -0.6805515268472178 1103 21 0.020434311667827387 1103 22 -0.6953533623261094 1103 23 0.20628777785238128 1103 24 0.33604214855946424 1103 25 0.32162610891742816 1104 1 0.49490895375764765 1104 2 0.4240947981037735 1104 3 0.7170306811467562 1104 4 0.27331605891921384 1104 5 0.11010297924485712 1104 6 0.24219203119437083 1104 7 0.15917356139402017 1104 8 0.3678019717654166 1104 9 0.8204912907146777 1104 10 0.3106251996090137 1104 11 0.5404605184872793 1104 12 0.6965402182829123 1104 13 0.7833538994570901 1104 14 0.2290592658489956 1104 15 0.5951951719949449 1104 16 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0.655527385271867 2207 8 0.6824919809795773 2207 9 -0.1945343773121735 2207 10 0.7108851706502151 2207 11 0.45382434631405033 2207 12 0.7794380726991292 2207 13 0.9415775403679731 2207 14 0.9528482615051743 2207 15 1.0430068081671209 2207 16 1.2650761935675305 2207 17 1.1473138728209022 2207 18 0.9803254624769693 2207 19 -0.03389264824095761 2207 20 0.6117152315981897 2207 21 0.06338941068296577 2207 22 0.8063410144609335 2207 23 -0.030594039366725667 2207 24 -1.1330619497143886 2207 25 -0.009253908026337854 2208 1 0.542968845131135 2208 2 -0.09156470834694375 2208 3 0.860267620684521 2208 4 0.8827107841724313 2208 5 0.7408185115310673 2208 6 0.19354823154993994 2208 7 0.5070132638943932 2208 8 0.4709417019932687 2208 9 0.11842803942209201 2208 10 0.32972780762388965 2208 11 0.16207164862705525 2208 12 0.3504228820789663 2208 13 0.5014827833441586 2208 14 0.6974743952711222 2208 15 0.6973986917548558 2208 16 0.21248051478278285 2208 17 0.31718826753122187 2208 18 0.5008991664810978 2208 19 -1.9140821622885937 2208 20 -0.5213839744583879 2208 21 -0.7164602808343687 2208 22 0.08079840452558074 2208 23 0.21227460355537886 2208 24 0.3544881326295908 2208 25 0.8000276100524673 2209 1 -0.4662566791315257 2209 2 -2.0732853700870884 2209 3 -0.9026005027095962 2209 4 -0.4270701447488854 2209 5 -0.36181115313439705 2209 6 -0.166100480466973 2209 7 -0.2611497796856739 2209 8 0.013427504676322144 2209 9 -2.43601816375174 2209 10 -0.7572262708071165 2209 11 -0.15634786764951514 2209 12 0.19461923120652358 2209 13 0.0863248255099549 2209 14 -0.13231702358641684 2209 15 0.23917892134709717 2209 16 0.32848797013031117 2209 17 0.14300537424446702 2209 18 0.3307374154110292 2209 19 -0.08786856096236306 2209 20 -0.057553961377188045 2209 21 0.3469975892714956 2209 22 0.19726167257778826 2209 23 0.13488946991796524 2209 24 0.15520432053681787 2209 25 0.04532040727512765 2210 1 0.3096824979704422 2210 2 0.25274325245316476 2210 3 -0.017659861955003464 2210 4 0.3689535989492754 2210 5 -0.12661030746843238 2210 6 -0.09978888508560857 2210 7 0.1844104740141148 2210 8 0.40491392523250624 2210 9 0.6755040349797596 2210 10 -1.1491192615373151 2210 11 -0.5020138295945458 2210 12 -2.329409066077588 2210 13 -0.5894354607154497 2210 14 -0.6119478578684081 2210 15 -0.753543472538091 2210 16 0.009289726513458163 2210 17 0.6684160101202252 2210 18 0.6548551581334294 2210 19 0.83764778315863 2210 20 0.7033014622689322 2210 21 0.5884189585998385 2210 22 0.4483187550302219 2210 23 0.43676782412190107 2210 24 0.27863902536687135 2210 25 -0.12852899913241345 2211 1 -0.3245031487850376 2211 2 -0.1064817869394723 2211 3 -0.9093463877197401 2211 4 -0.27052563689867526 2211 5 -0.5070292367700446 2211 6 -0.3267458201431026 2211 7 -0.252831520490453 2211 8 0.25372758829198006 2211 9 0.5808282266168021 2211 10 -0.198054032539126 2211 11 -0.27339979930454206 2211 12 -0.18793740924279945 2211 13 -0.25119595348152757 2211 14 -0.726404630146412 2211 15 -0.04897197327385072 2211 16 0.026065780999012134 2211 17 -0.01897248592749179 2211 18 0.5144220344600754 2211 19 0.19508550372484024 2211 20 0.3812650257073571 2211 21 0.28367621530961173 2211 22 0.18876685441686902 2211 23 -0.20715854745810702 2211 24 0.2578683621381046 2211 25 0.33478050328323405 2212 1 -0.47075101538527164 2212 2 -0.5746177167484134 2212 3 -2.1758384954657046 2212 4 -1.232999002528942 2212 5 -0.7369558442845587 2212 6 -0.4749265557640783 2212 7 -0.4917051560863399 2212 8 -0.5733142033751264 2212 9 -1.21405090723173 2212 10 -0.5989706435518869 2212 11 -0.47818850880528174 2212 12 -0.1709020133580097 2212 13 -0.3775320889449034 2212 14 -0.33386967419250624 2212 15 0.13916296713229315 2212 16 -0.8906618399466453 2212 17 -1.2928603795242921 2212 18 -0.169138684835751 2212 19 -0.16167547422914197 2212 20 -0.2767479001333726 2212 21 -0.15102850096147336 2212 22 -0.7613354427643912 2212 23 0.09057748694484502 2212 24 -0.3205435757132662 2212 25 -0.24965265725797536 2213 1 -2.7486691503812075 2213 2 -1.3519087621988533 2213 3 -2.4071170714122125 2213 4 -0.94722688723121 2213 5 -0.8365636874218154 2213 6 -0.9623829576796794 2213 7 -0.451809972206334 2213 8 -0.6087890384704551 2213 9 -0.26808903861331146 2213 10 -0.48403434645240395 2213 11 -0.6210348086155264 2213 12 -0.31713134379986485 2213 13 -0.8628054240854155 2213 14 -0.06156823748879248 2213 15 -0.5160642850589028 2213 16 -1.9147695475458164 2213 17 -1.1584138267096122 2213 18 -0.6914518681736013 2213 19 -0.17599191048105922 2213 20 -0.2452633062958398 2213 21 -0.4388138086329292 2213 22 -0.7141754174639487 2213 23 -0.35213327477919876 2213 24 -0.22553679880895317 2213 25 -0.6636871742905939 2214 1 -0.5550891845303241 2214 2 -0.9719732660880567 2214 3 -6.610630636200678 2214 4 -1.6067450052353855 2214 5 -0.9703751084045464 2214 6 -0.11722264654327574 2214 7 -0.09168096442792262 2214 8 -0.22730544901873628 2214 9 0.2956597756534265 2214 10 0.17845853914805332 2214 11 -0.3953774380975972 2214 12 -0.21996572057846464 2214 13 -4.728341375517779 2214 14 -3.0261442192759276 2214 15 -0.6664721772994682 2214 16 -2.2755360055234277 2214 17 -2.8440446368025754 2214 18 -0.7451949909207087 2214 19 -0.29404920511206 2214 20 0.062131874884201466 2214 21 0.11970639626178325 2214 22 -0.053192681740178216 2214 23 0.25235164269376753 2214 24 0.6684643394949745 2214 25 0.2889897371114216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90.0 3 19.0 4 15.0 5 36.0 6 5.0 7 4.0 8 9.0 9 40.0 10 82.0 11 252.0 12 627.0 13 1152.0 14 1923.0 15 2769.0 16 3396.0 17 3949.0 18 4034.0 19 3948.0 20 3673.0 21 3413.0 22 3312.0 23 3169.0 24 3016.0 25 3020.0 26 3179.0 27 3334.0 28 3674.0 29 4439.0 30 5309.0 31 6741.0 32 8841.0 33 11544.0 34 15643.0 35 21571.0 36 29846.0 37 44137.0 38 76231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4527883086928 21.657234104956714 12.795333326091443 22.09464426025904 2 16.891172957909596 24.030255872027794 36.42104882197532 22.657522348087294 3 19.12739775606225 28.819761129207382 27.495114006514658 24.55772710821571 4 12.891632978781157 20.830151532150367 33.420674529787085 32.85754095928139 5 13.340281773734173 40.98638901856898 30.86012282816 14.813206379536842 6 29.12488869261788 39.40317524668612 16.531770565622487 14.940165495073519 7 25.421961928410354 37.23400019546167 19.231256492766107 18.112781383361867 8 23.844978861411942 37.851465222679096 19.678070886662418 18.625485029246537 9 24.025482779215608 20.97877038350859 19.62210196731399 35.373644869961815 10 16.465469498267954 32.90958253548249 28.28753760004054 22.337410366209014 11 30.874001469518355 29.846786423966904 20.492180063051748 18.787032043462997 12 20.717088213577334 31.346730176195507 26.696804737154523 21.239376873072636 13 26.650523389653824 27.286119677424026 24.456340760688587 21.607016172233564 14 20.614754246705253 28.25610899364035 25.354631107636287 25.774505652018114 15 23.824416235019022 32.347692630679745 22.898138395103288 20.929752739197948 16 20.892345891712484 32.94737642200208 23.65111136045287 22.50916632583257 17 20.359725771539026 31.617716258967803 25.21663903629111 22.80591893320206 18 20.24064432681543 30.000833306160306 27.82373038560337 21.934791981420894 19 21.579416044654558 30.788332476633823 27.607926039079977 20.024325439631642 20 22.375288658525108 28.385539203266276 28.395673922643134 20.843498215565482 21 23.810075358877654 28.693562281470115 26.82913587039142 20.667226489260816 22 23.36537904673655 28.94574911681242 26.33346846585973 21.355403370591304 23 23.15187843644011 28.92636471239779 27.07815265666909 20.84360419449301 24 22.81588369239012 28.20336455094069 27.24439151006578 21.73636024660341 25 22.353179659446308 29.537819993049926 27.58130140159852 20.52769894590525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2393.0 1 2393.0 2 2023.0 3 1653.0 4 1653.0 5 1653.0 6 1756.0 7 1859.0 8 1859.0 9 1859.0 10 2054.0 11 2249.0 12 2249.0 13 2249.0 14 2605.5 15 2962.0 16 2962.0 17 2962.0 18 4193.0 19 5424.0 20 5424.0 21 5424.0 22 7429.0 23 9434.0 24 9434.0 25 9434.0 26 12123.5 27 14813.0 28 14813.0 29 14813.0 30 17314.0 31 19815.0 32 19815.0 33 19815.0 34 21743.0 35 23671.0 36 23671.0 37 23671.0 38 24222.0 39 24773.0 40 24773.0 41 24773.0 42 26896.5 43 29020.0 44 29020.0 45 29020.0 46 32007.5 47 34995.0 48 34995.0 49 34995.0 50 33380.5 51 31766.0 52 31766.0 53 31766.0 54 28481.5 55 25197.0 56 25197.0 57 25197.0 58 21894.0 59 18591.0 60 18591.0 61 18591.0 62 16461.5 63 14332.0 64 14332.0 65 14332.0 66 10602.0 67 6872.0 68 6872.0 69 6872.0 70 5452.0 71 4032.0 72 4032.0 73 4032.0 74 2889.0 75 1746.0 76 1746.0 77 1746.0 78 1173.0 79 600.0 80 600.0 81 600.0 82 397.0 83 194.0 84 194.0 85 194.0 86 116.0 87 38.0 88 38.0 89 38.0 90 25.0 91 12.0 92 12.0 93 12.0 94 6.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09803141346105151 2 0.04774961836479261 3 0.05136701369545872 4 0.047387878831726 5 0.0437704835010599 6 0.06511311595198993 7 0.06258093922052366 8 0.061495720621323816 9 0.0640278973527901 10 0.06475137641892333 11 0.058240064823724326 12 0.0571548462245245 13 0.060048762489057377 14 0.06113398108825722 15 0.06330441828665687 16 0.05751658575759111 17 0.06366615781972348 18 0.15663321781784245 19 0.06800703221652282 20 0.05932528342292416 21 0.05932528342292416 22 0.061495720621323816 23 0.06330441828665687 24 0.07560356241092164 25 0.06728355315038959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 276442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.6198069521967 #Duplication Level Percentage of deduplicated Percentage of total 1 87.77255933231999 57.596183990870685 2 7.8912812717007785 10.356487073089808 3 1.9329057271689705 3.805107020208697 4 0.7827049051206906 2.0544377909822864 5 0.3925776672122299 1.2880435368105123 6 0.2572748917130065 1.012939723671288 7 0.1499792036874357 0.6889124474969602 8 0.10740676648116311 0.5638409025482874 9 0.10060148377992334 0.5941304950268805 >10 0.4785925525923266 5.972702007803267 >50 0.06457446581131845 3.0451073802321504 >100 0.0640225473001106 8.430543482046145 >500 0.0027595925560392506 1.3631998310173425 >1k 0.0027595925560392506 3.228364318195694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2441 0.8830062002155967 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2132 0.7712286844980141 No Hit GGTATCAACGCAGAGTACTTTTTTT 1679 0.6073606760188394 No Hit TATCAACGCAGAGTACTTTTTTTTT 1450 0.5245223229465855 No Hit GTACTGGTTCACTATCGGTCAGTCA 1212 0.43842831407673216 No Hit ATACAGGGTGACAGCCCCGTACACA 988 0.3573986586698114 No Hit GGATACCACGTGTCCCGCCCTACTC 838 0.30313772870981975 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 720 0.2604524638079597 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 678 0.24525940341916208 No Hit GTTAATGATAGTGTGTCGAAACACA 540 0.1953393478559698 No Hit GTTCACTATCGGTCAGTCAGGAGTA 471 0.17037932007437365 No Hit GGTATCAACGCAGAGTACATGGGGG 467 0.1689323619421072 No Hit GTATTTAGCCTTGGAGGATGGTCCC 423 0.15301582248717632 No Hit GGTTAATGAGGCGAACCGGGGGAAC 406 0.14686625042504395 No Hit CCCATATTCAGACAGGATACCACGT 405 0.14650451089197736 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 399 0.14433407369357767 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 389 0.14071667836291157 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 388 0.14035493882984496 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 364 0.1316731900362463 No Hit GCCTTGGAGGATGGTCCCCCCATAT 362 0.13094971097011307 No Hit CACACACACTGATTCAGGCTCTGGG 343 0.12407665984184749 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 334 0.12082100404424798 No Hit TCACGGTACTGGTTCACTATCGGTC 324 0.11720360871358187 No Hit AGCGTACACGGTGGATGCCCTGGCA 321 0.11611839011438205 No Hit CTATCGGTCAGTCAGGAGTATTTAG 317 0.1146714319821156 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 315 0.11394795291598236 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 312 0.11286273431678254 No Hit ATCATTAACTGAATCCATAGGTTAA 311 0.11250099478371595 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 306 0.11069229711838288 No Hit GGGTTTCCCCATTCGGAAATCGCCG 303 0.10960707851918304 No Hit CATCTAAGTACCCCGAGGAAAAGAA 290 0.1049044645893171 No Hit CGGTACTGGTTCACTATCGGTCAGT 287 0.10381924599011727 No Hit GTATCAACGCAGAGTACATGGGGGT 286 0.10345750645705068 No Hit TATTCAGACAGGATACCACGTGTCC 284 0.10273402739091744 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGAA 25 0.006032075 18.9933 1 GGTATCA 355 0.0 15.515653 1 CGAATGG 65 3.347579E-6 14.615524 19 AGTATGT 40 0.005238961 14.260469 17 GTACATA 50 0.0014980844 13.295309 1 CCTTGGG 50 0.0015001311 13.292902 2 CCGAATG 65 5.3561824E-5 13.170674 18 ACCGGGG 135 3.601599E-10 11.971752 15 CCGGGGG 130 2.5411282E-9 11.700898 16 CGGGGGA 135 4.5929482E-9 11.267531 17 ACAGGGT 155 3.092282E-10 11.02637 3 GGGGAAC 150 2.0918378E-9 10.766768 19 TGTCCCG 195 1.8189894E-12 10.712229 11 CAGGGTG 180 3.45608E-11 10.549923 4 GTACTGG 310 0.0 10.41568 1 GTATCAA 760 0.0 10.246386 1 AACCGGG 160 5.980837E-9 10.101166 14 CAGAAGG 85 6.588701E-4 10.053455 4 AGGGTGA 180 3.8926373E-10 10.022427 5 AGAGGCG 95 1.6325741E-4 10.000095 19 >>END_MODULE