FastQCFastQC Report
Wed 25 May 2016
SRR1294646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276442
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75512.731495214185977No Hit
GGTATCAACGCAGAGTACTTTTTTT55902.022123989842354No Hit
TATCAACGCAGAGTACTTTTTTTTT47151.7056018984090697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28511.0313194087729072No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14630.5292249368764516No Hit
GTACTGGTTCACTATCGGTCAGTCA12460.4507274582009969No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12380.44783354193646413No Hit
ATACAGGGTGACAGCCCCGTACACA10430.377294332988475No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9640.34871690987621273No Hit
GGATACCACGTGTCCCGCCCTACTC8240.2980733752468872No Hit
ATCAACGCAGAGTACTTTTTTTTTT8030.29047684505248844No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6730.243450705753829No Hit
GTGGTATCAACGCAGAGTACTTTTT6440.2329602592948973No Hit
GTTAATGATAGTGTGTCGAAACACA5340.19316891065757014No Hit
GGTATCAACGCAGAGTACATGGGGG5160.18665759906237114No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4390.1588036550162421No Hit
GCCTTGGAGGATGGTCCCCCCATAT4370.1580801759501089No Hit
GTTCACTATCGGTCAGTCAGGAGTA4180.1512071248218433No Hit
CCCATATTCAGACAGGATACCACGT4070.14722798995811054No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3960.14324885509437785No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3900.1410784178959782No Hit
GTATTTAGCCTTGGAGGATGGTCCC3870.13999319929677834No Hit
CACACACACTGATTCAGGCTCTGGG3830.13854624116451192No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3750.13565232489997903No Hit
CTATCGGTCAGTCAGGAGTATTTAG3620.13094971097011307No Hit
CGGTACTGGTTCACTATCGGTCAGT3470.1255236179741139No Hit
TCTAAGTACCCCGAGGAAAAGAAAT3460.12516187844104731No Hit
AGCGTACACGGTGGATGCCCTGGCA3450.1248001389079807No Hit
CAACGCAGAGTACTTTTTTTTTTTT3360.12154448311038121No Hit
TCACGGTACTGGTTCACTATCGGTC3280.11865056684584832No Hit
CATCTAAGTACCCCGAGGAAAAGAA3260.1179270877797151No Hit
GGTTAATGAGGCGAACCGGGGGAAC3240.11720360871358187No Hit
TATTCAGACAGGATACCACGTGTCC3170.1146714319821156No Hit
GTGGATGCCCTGGCAGTCAGAGGCG3060.11069229711838288No Hit
ATCATTAACTGAATCCATAGGTTAA2990.10816012038691661No Hit
AGCTCACAGCATGTGCATTTTTGTG2930.10598968318851693No Hit
AACGCAGAGTACTTTTTTTTTTTTT2900.1049044645893171No Hit
GGGTTTCCCCATTCGGAAATCGCCG2870.10381924599011727No Hit
GGTATCAACGCAGAGTACATGGGGA2810.10164880879171762No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2800.101287069258651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATA307.5726013E-419.0474388
GGTCTAG250.00597741919.0232331
CGAATGG307.8071636E-418.9612819
GGTATCA8800.015.8887231
ACACGGT500.001466268613.3332076
CCAGAAC751.4716359E-512.6637663
CAGAACC701.0596622E-412.24478154
TACACGG701.0596622E-412.24478155
AACCAGA701.0723257E-412.2292221
AGGGTGA1751.8189894E-1211.4284635
TAACCTA1001.8539995E-611.4284635
ACCAGAA1001.9157942E-611.3973892
GGTGACA1701.0913936E-1111.2043747
TACTCTG600.005747175311.1110057
TGTCCCG1803.6379788E-1211.06475211
AAATCGC700.001510172310.83501817
GTGTCCC1855.456968E-1210.81070810
TTAACCT1158.439547E-710.7659434
GTACACG803.664041E-410.7141844
GTATCAA19950.010.6797111
Produced by FastQC (version 0.11.5)