##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294646_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 276442 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.046671634556255 31.0 31.0 34.0 28.0 34.0 2 31.112066907344037 31.0 31.0 34.0 28.0 34.0 3 31.164233365407572 31.0 31.0 34.0 27.0 34.0 4 34.725674825098935 37.0 35.0 37.0 32.0 37.0 5 34.99901245107473 37.0 35.0 37.0 32.0 37.0 6 34.44713176724232 37.0 35.0 37.0 30.0 37.0 7 34.2838606289927 37.0 35.0 37.0 30.0 37.0 8 34.26257949226239 37.0 35.0 37.0 30.0 37.0 9 35.54468930191505 39.0 35.0 39.0 29.0 39.0 10 35.35576721337568 39.0 35.0 39.0 28.0 39.0 11 35.530878086542565 39.0 35.0 39.0 29.0 39.0 12 35.53020886840639 39.0 35.0 39.0 29.0 39.0 13 35.3108029894155 39.0 35.0 39.0 27.0 39.0 14 36.20278756484181 39.0 36.0 41.0 27.0 41.0 15 36.23234530208868 39.0 36.0 41.0 27.0 41.0 16 36.28873687789844 39.0 36.0 41.0 27.0 41.0 17 36.45064425810839 39.0 36.0 41.0 28.0 41.0 18 36.5328170104398 39.0 36.0 41.0 29.0 41.0 19 36.591766084748336 39.0 36.0 41.0 28.0 41.0 20 36.67317556666498 39.0 36.0 41.0 29.0 41.0 21 36.55744423785098 39.0 36.0 41.0 28.0 41.0 22 36.538192459901175 39.0 36.0 41.0 28.0 41.0 23 36.55208325797093 39.0 36.0 41.0 28.0 41.0 24 36.43700667771178 39.0 35.0 41.0 28.0 41.0 25 35.235825236396785 38.0 33.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4753383264384752 1101 2 0.39985824908775314 1101 3 0.601657667784 1101 4 0.5512439986992348 1101 5 0.42457443002347617 1101 6 0.5605448639300619 1101 7 0.7276038422128153 1101 8 0.5908951513401917 1101 9 0.7593953917144418 1101 10 0.7799625682003608 1101 11 0.9046447487761213 1101 12 0.9541856212952453 1101 13 1.0419789468223044 1101 14 1.0018266348359433 1101 15 0.8442381790100058 1101 16 0.8619456955614879 1101 17 1.0461696876574322 1101 18 1.0109310694203941 1101 19 1.2927210232623167 1101 20 1.2127353844133424 1101 21 1.1718930260680125 1101 22 1.3480267412724487 1101 23 1.208613016960804 1101 24 1.2638970997306842 1101 25 0.8200783277900427 1102 1 0.4632156404344663 1102 2 0.3121327450198379 1102 3 0.22093163311827624 1102 4 0.4103260644933613 1102 5 0.46907832107405767 1102 6 0.37807080426728845 1102 7 0.3517416207766644 1102 8 0.436343801263547 1102 9 0.504995851565873 1102 10 0.933924365158262 1102 11 0.5084797918134996 1102 12 0.7645632300678002 1102 13 0.6770087192548004 1102 14 1.1379691887800547 1102 15 0.78311625942505 1102 16 1.0903385737598157 1102 17 0.6761071950268445 1102 18 0.8102398183886805 1102 19 0.8385808864859925 1102 20 0.8699913094221827 1102 21 1.0349766836555645 1102 22 1.108432353818138 1102 23 0.8394201151803529 1102 24 0.9033411511397134 1102 25 1.0039746843517676 1103 1 0.7831774768436723 1103 2 0.5330828475488865 1103 3 0.5022457137744603 1103 4 0.5411360439875494 1103 5 0.5616535090439854 1103 6 0.5489667942422258 1103 7 0.6819278818839791 1103 8 0.7000713018331552 1103 9 0.8585074715248595 1103 10 0.9549428203872452 1103 11 1.0669498305466902 1103 12 0.9831637085366935 1103 13 0.8168611913441168 1103 14 1.2886014512544222 1103 15 0.950722775715775 1103 16 1.2030635680637545 1103 17 0.858324100927959 1103 18 0.8517783237429768 1103 19 0.9696614287507472 1103 20 1.139770871964906 1103 21 1.4292436009487943 1103 22 1.3814444294617942 1103 23 1.2734882400379561 1103 24 1.5755614746758297 1103 25 1.4736431737571039 1104 1 0.4031866118306695 1104 2 0.28681413839282044 1104 3 0.3473866033816577 1104 4 0.28001595404306556 1104 5 0.45213906873760834 1104 6 0.34555522794330074 1104 7 0.4487573387035937 1104 8 0.5022882929089789 1104 9 0.33699457713936454 1104 10 1.020967695659536 1104 11 0.9779855975342571 1104 12 0.9585203243753568 1104 13 0.41477068470368295 1104 14 0.46598533151582444 1104 15 0.8472128326337725 1104 16 0.6664076759971422 1104 17 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0.059702259666345014 2207 7 0.1432749114296712 2207 8 0.2251295460352125 2207 9 0.536125813153582 2207 10 0.3684634176550645 2207 11 0.16879776023005633 2207 12 0.1218775706567854 2207 13 0.44288046488006927 2207 14 0.5347361542089928 2207 15 0.5202054613029787 2207 16 0.734171266888282 2207 17 0.7824345531147685 2207 18 0.46514272065073925 2207 19 0.7476451203741732 2207 20 0.5253210149279468 2207 21 0.9407865428104927 2207 22 0.741221513015752 2207 23 0.5475933972972982 2207 24 1.129066716654009 2207 25 1.4487570146974775 2208 1 -0.011528529008657529 2208 2 0.21725728888783635 2208 3 -0.0654300200627489 2208 4 -0.11603043241287025 2208 5 -0.020777699868354205 2208 6 -0.14668923063673134 2208 7 0.030039653459510873 2208 8 -0.047671746888127586 2208 9 0.23059278953191864 2208 10 0.06969304673846466 2208 11 -0.21020575475163383 2208 12 -0.3888855244935243 2208 13 -0.3081273270723415 2208 14 -0.711447755532177 2208 15 -0.12809665506011925 2208 16 0.01949929087864888 2208 17 0.16531868162052632 2208 18 0.18171966925334715 2208 19 0.4821533916422709 2208 20 0.12196457612058254 2208 21 0.7120054794670807 2208 22 0.6515864074433964 2208 23 0.6437791505603343 2208 24 0.6882253332752057 2208 25 0.49518918538302614 2209 1 -0.3485864141001471 2209 2 -0.29837302827663237 2209 3 -0.19658544282903279 2209 4 -0.9406049812051265 2209 5 -0.08470074869338617 2209 6 -0.2961614557207852 2209 7 -0.011660965009646418 2209 8 0.1485056704021659 2209 9 -0.24718946311944023 2209 10 0.31614098574697636 2209 11 -0.14300147885381875 2209 12 0.051754236735547465 2209 13 -0.12601141952114858 2209 14 -0.008172460268575321 2209 15 -0.7458789701515798 2209 16 -0.5786861999161204 2209 17 -0.3515666814842433 2209 18 -1.0108455884525398 2209 19 -0.4812848098331912 2209 20 -0.016321505669701253 2209 21 0.36648383929063044 2209 22 0.35597938043493116 2209 23 0.07495299315509385 2209 24 0.24513792026206715 2209 25 -0.05158312992697489 2210 1 -0.2196223549035281 2210 2 -0.4658392829521887 2210 3 -0.21394598925934716 2210 4 -0.4874012082339121 2210 5 -0.17996014046440223 2210 6 -0.7494058857094004 2210 7 -0.6111080295729892 2210 8 -0.8427937278739819 2210 9 -0.29614051207049386 2210 10 -0.20593577470187796 2210 11 -0.47199806366435837 2210 12 -0.23499970699513995 2210 13 -0.27855654881046377 2210 14 -0.3191986433877787 2210 15 -0.15074154943275886 2210 16 -0.07457984115860228 2210 17 -1.4421911732715245 2210 18 -1.8067473610985019 2210 19 -0.7626720272669161 2210 20 -0.44163456470369056 2210 21 -0.6883624771835954 2210 22 -1.0092832638974798 2210 23 -0.3123864116261714 2210 24 0.12682599962456464 2210 25 -0.21193440655731877 2211 1 -0.2965714765453882 2211 2 -0.3811125571640126 2211 3 -0.2893421945438206 2211 4 -0.10721668140134 2211 5 -0.06153154857212684 2211 6 -0.024047445267164846 2211 7 -0.2427966389152303 2211 8 -0.18203270119116866 2211 9 0.017385812629015618 2211 10 -0.09371233705944348 2211 11 -0.26016334344974723 2211 12 -0.14341670898755865 2211 13 -0.49635550962618424 2211 14 -0.018349585555483827 2211 15 -0.3465392340572606 2211 16 -0.04060776109713515 2211 17 -0.34649310969636815 2211 18 -0.24381680934113348 2211 19 -0.3566641617618487 2211 20 -0.46394512193045045 2211 21 -0.1304097223034688 2211 22 0.091757222617872 2211 23 -0.5989792415150887 2211 24 -0.128041107197852 2211 25 -0.4509728355398863 2212 1 -0.22853943402933652 2212 2 -0.22417914043815657 2212 3 -0.317331692739927 2212 4 0.05757895391892731 2212 5 -0.10694160302461597 2212 6 -0.03223121976813559 2212 7 -0.33215737870582984 2212 8 -0.499758205521573 2212 9 -0.4570858286135291 2212 10 -0.839881051436528 2212 11 -0.33597279860683216 2212 12 -0.6943055813864021 2212 13 -0.36113241696508425 2212 14 -0.43847121142695045 2212 15 -0.5186705843447186 2212 16 -0.6909563489013308 2212 17 -0.5515680392897551 2212 18 -0.5346835615204668 2212 19 -0.6389973148825305 2212 20 -0.950939775621805 2212 21 -0.33824437896303294 2212 22 -0.7161608265625148 2212 23 -0.7198418594041982 2212 24 -0.43803513544530404 2212 25 -0.2957708736954956 2213 1 -0.21043154571271927 2213 2 -0.12197980909271422 2213 3 -0.16572421103756696 2213 4 -0.2881371389698444 2213 5 -0.18937405987832534 2213 6 -0.5527625290660438 2213 7 -0.684914223379181 2213 8 -0.5750414801217332 2213 9 -0.6112920272220066 2213 10 -0.5544306131967147 2213 11 -0.3392907709570636 2213 12 0.024617342621908733 2213 13 0.030720840466919697 2213 14 -0.7160351402242782 2213 15 -0.2799789786701936 2213 16 -0.13295146953022652 2213 17 0.05714615606580509 2213 18 -0.257043731394873 2213 19 -0.34818318277807236 2213 20 -0.7393268723959991 2213 21 -0.17085664967776637 2213 22 -0.6661996808138966 2213 23 -0.8695192787590358 2213 24 -0.7704034376048767 2213 25 -0.30630670092961765 2214 1 -0.017716690846469874 2214 2 0.048097975008971616 2214 3 0.09685973887705757 2214 4 0.1088501499696406 2214 5 0.055482703544178946 2214 6 -0.14176651312979516 2214 7 0.16093665880636365 2214 8 0.40353563996933417 2214 9 0.1475393140794523 2214 10 0.2712115799355175 2214 11 0.4314092859581322 2214 12 0.15017985447924076 2214 13 -0.14293121796600872 2214 14 -0.2890952806150935 2214 15 0.11561957947817092 2214 16 -0.13536467385578277 2214 17 -0.6896419296864238 2214 18 -0.08047761490843186 2214 19 -0.4458344823417235 2214 20 -0.2204424102446012 2214 21 -0.8671647506073867 2214 22 -1.135742462069821 2214 23 -0.9633838205917087 2214 24 -0.29494147592777153 2214 25 -0.6835026789224088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 8.0 10 17.0 11 37.0 12 67.0 13 226.0 14 429.0 15 731.0 16 1057.0 17 1385.0 18 1629.0 19 1759.0 20 1929.0 21 1938.0 22 1915.0 23 2028.0 24 2195.0 25 2320.0 26 2602.0 27 2746.0 28 3278.0 29 4014.0 30 5250.0 31 6548.0 32 9034.0 33 11953.0 34 16491.0 35 22210.0 36 31197.0 37 49243.0 38 92205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38671132434145 20.20204580429076 12.630759482212364 22.780483389155425 2 17.111365132649887 22.410849292075735 36.72452087598845 23.753264699285925 3 19.06653965540347 27.325773024111616 29.66014828444245 23.947539036042464 4 12.4528793349203 19.271538032979038 34.773061088641114 33.502521543459544 5 12.961991425004976 38.62908623839934 32.22099607431662 16.187926262279067 6 29.763239965556913 37.961562674114866 16.199465987452875 16.075731372875346 7 25.144277562894164 36.399917503989116 19.883709570622738 18.572095362493986 8 25.77465807945582 34.542658658368914 19.818366017801576 19.864317244373687 9 25.79492262391388 15.954883845435933 21.112927847432736 37.13726568321746 10 17.62563279417212 29.92453679829754 29.12523695735857 23.324593450171772 11 31.812916723979413 26.381116476388357 21.417712171778962 20.388254627853268 12 22.65374041383302 28.670235855881927 28.52264505860223 20.153378671682827 13 29.19093336034322 23.54164707244196 26.194644807952482 21.07277475926234 14 22.175718595582435 24.866698982064957 28.184573979351907 24.773008443000702 15 24.94637192022891 28.40316740280928 26.193654342156194 20.45680633480562 16 20.235709479746202 30.035233430520687 27.140231947388603 22.588825142344508 17 19.81225582404862 28.35624366951237 28.17935175806685 23.65214874837216 18 19.57662005049884 25.43788570477713 32.667250273113346 22.31824397161068 19 20.643826349926385 26.348117681530596 34.02823749009735 18.979818478445672 20 21.210597521360718 23.973202335390425 34.760636950969825 20.05556319227903 21 22.004977535974994 25.0059687022956 33.356002344072174 19.633051417657228 22 21.64848879483423 24.946551630582235 33.04176388662796 20.363195687955578 23 21.705095463062776 24.993669558171334 33.581366073172674 19.719868905593216 24 21.616469277461455 24.344347096316767 33.54338342220068 20.495800204021098 25 21.2519127047001 25.519731154206504 33.81481039353786 19.413545747555535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 411.0 1 411.0 2 520.0 3 629.0 4 629.0 5 629.0 6 1365.5 7 2102.0 8 2102.0 9 2102.0 10 2172.0 11 2242.0 12 2242.0 13 2242.0 14 2102.5 15 1963.0 16 1963.0 17 1963.0 18 3370.0 19 4777.0 20 4777.0 21 4777.0 22 7443.5 23 10110.0 24 10110.0 25 10110.0 26 13740.5 27 17371.0 28 17371.0 29 17371.0 30 20383.0 31 23395.0 32 23395.0 33 23395.0 34 24760.0 35 26125.0 36 26125.0 37 26125.0 38 24758.5 39 23392.0 40 23392.0 41 23392.0 42 25477.0 43 27562.0 44 27562.0 45 27562.0 46 30757.5 47 33953.0 48 33953.0 49 33953.0 50 32433.0 51 30913.0 52 30913.0 53 30913.0 54 27939.0 55 24965.0 56 24965.0 57 24965.0 58 21606.5 59 18248.0 60 18248.0 61 18248.0 62 16379.0 63 14510.0 64 14510.0 65 14510.0 66 10713.5 67 6917.0 68 6917.0 69 6917.0 70 5553.5 71 4190.0 72 4190.0 73 4190.0 74 2975.0 75 1760.0 76 1760.0 77 1760.0 78 1198.5 79 637.0 80 637.0 81 637.0 82 427.5 83 218.0 84 218.0 85 218.0 86 128.5 87 39.0 88 39.0 89 39.0 90 25.5 91 12.0 92 12.0 93 12.0 94 6.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09658445532878507 2 0.0 3 0.12697057610638038 4 0.008681748793598657 5 0.02061915338479681 6 0.01591653945493087 7 0.023513069649329695 8 0.022427851050129864 9 0.0 10 0.3892317375796731 11 0.06511311595198993 12 7.234790661332215E-4 13 0.0 14 0.0 15 3.6173953306661074E-4 16 0.0 17 7.234790661332215E-4 18 0.0 19 3.6173953306661074E-4 20 0.0 21 0.0 22 0.002532176731466275 23 0.0 24 0.0 25 3.6173953306661074E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 276442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.3952794455641 #Duplication Level Percentage of deduplicated Percentage of total 1 85.97381556523823 50.204649849334736 2 9.207446124053378 10.75342778788146 3 2.1295893172472247 3.7307388985481924 4 0.8514312713412405 1.9887826807465456 5 0.44011083254077893 1.2850197526619322 6 0.2759840166390113 0.9669698264486566 7 0.16969054906597159 0.6936388922385031 8 0.13208654025551717 0.6170584343374941 9 0.09838826457018335 0.5170869183365955 >10 0.562105971760565 6.172922505251761 >50 0.07469603779118364 3.1282064148764985 >100 0.07531850477277684 8.798671215256858 >500 0.004357268871152379 1.8021900307875527 >1k 0.0037348018895591814 4.563997181639474 >5k 0.0012449339631863939 4.776639611653737 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7551 2.731495214185977 No Hit GGTATCAACGCAGAGTACTTTTTTT 5590 2.022123989842354 No Hit TATCAACGCAGAGTACTTTTTTTTT 4715 1.7056018984090697 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2851 1.0313194087729072 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1463 0.5292249368764516 No Hit GTACTGGTTCACTATCGGTCAGTCA 1246 0.4507274582009969 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1238 0.44783354193646413 No Hit ATACAGGGTGACAGCCCCGTACACA 1043 0.377294332988475 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 964 0.34871690987621273 No Hit GGATACCACGTGTCCCGCCCTACTC 824 0.2980733752468872 No Hit ATCAACGCAGAGTACTTTTTTTTTT 803 0.29047684505248844 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 673 0.243450705753829 No Hit GTGGTATCAACGCAGAGTACTTTTT 644 0.2329602592948973 No Hit GTTAATGATAGTGTGTCGAAACACA 534 0.19316891065757014 No Hit GGTATCAACGCAGAGTACATGGGGG 516 0.18665759906237114 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 439 0.1588036550162421 No Hit GCCTTGGAGGATGGTCCCCCCATAT 437 0.1580801759501089 No Hit GTTCACTATCGGTCAGTCAGGAGTA 418 0.1512071248218433 No Hit CCCATATTCAGACAGGATACCACGT 407 0.14722798995811054 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 396 0.14324885509437785 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 390 0.1410784178959782 No Hit GTATTTAGCCTTGGAGGATGGTCCC 387 0.13999319929677834 No Hit CACACACACTGATTCAGGCTCTGGG 383 0.13854624116451192 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 375 0.13565232489997903 No Hit CTATCGGTCAGTCAGGAGTATTTAG 362 0.13094971097011307 No Hit CGGTACTGGTTCACTATCGGTCAGT 347 0.1255236179741139 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 346 0.12516187844104731 No Hit AGCGTACACGGTGGATGCCCTGGCA 345 0.1248001389079807 No Hit CAACGCAGAGTACTTTTTTTTTTTT 336 0.12154448311038121 No Hit TCACGGTACTGGTTCACTATCGGTC 328 0.11865056684584832 No Hit CATCTAAGTACCCCGAGGAAAAGAA 326 0.1179270877797151 No Hit GGTTAATGAGGCGAACCGGGGGAAC 324 0.11720360871358187 No Hit TATTCAGACAGGATACCACGTGTCC 317 0.1146714319821156 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 306 0.11069229711838288 No Hit ATCATTAACTGAATCCATAGGTTAA 299 0.10816012038691661 No Hit AGCTCACAGCATGTGCATTTTTGTG 293 0.10598968318851693 No Hit AACGCAGAGTACTTTTTTTTTTTTT 290 0.1049044645893171 No Hit GGGTTTCCCCATTCGGAAATCGCCG 287 0.10381924599011727 No Hit GGTATCAACGCAGAGTACATGGGGA 281 0.10164880879171762 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 280 0.101287069258651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAATA 30 7.5726013E-4 19.047438 8 GGTCTAG 25 0.005977419 19.023233 1 CGAATGG 30 7.8071636E-4 18.96128 19 GGTATCA 880 0.0 15.888723 1 ACACGGT 50 0.0014662686 13.333207 6 CCAGAAC 75 1.4716359E-5 12.663766 3 CAGAACC 70 1.0596622E-4 12.2447815 4 TACACGG 70 1.0596622E-4 12.2447815 5 AACCAGA 70 1.0723257E-4 12.229222 1 AGGGTGA 175 1.8189894E-12 11.428463 5 TAACCTA 100 1.8539995E-6 11.428463 5 ACCAGAA 100 1.9157942E-6 11.397389 2 GGTGACA 170 1.0913936E-11 11.204374 7 TACTCTG 60 0.0057471753 11.111005 7 TGTCCCG 180 3.6379788E-12 11.064752 11 AAATCGC 70 0.0015101723 10.835018 17 GTGTCCC 185 5.456968E-12 10.810708 10 TTAACCT 115 8.439547E-7 10.765943 4 GTACACG 80 3.664041E-4 10.714184 4 GTATCAA 1995 0.0 10.679711 1 >>END_MODULE