FastQCFastQC Report
Wed 25 May 2016
SRR1294645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267356
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC138690.3250021793354011No Hit
CCCATGTACTCTGCGTTGATACCAC133560.3129806840582318No Hit
GTATCAACGCAGAGTACTTTTTTTT64950.15220197236883917No Hit
ATTCCATTCCATTCCATTCCATTCC63620.14908528840809157No Hit
GAGTACATGGGAAGCAGTGGTATCA62040.1453827615975794No Hit
CATGTACTCTGCGTTGATACCACTG50530.1184105567944179No Hit
GAATGGAATGGAATGGAATGGAATG48590.11386441628024471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT907.4327345E-611.61618814
CTAGACT5150.010.142574
TCGCGCG1054.1245446E-59.9489119
TAGACTG7150.09.8292025
TATACTG10100.09.6851955
ACACCGT1304.2595148E-69.4971336
AGGACCG900.00111743219.4971335
GCGCGCC1201.7095601E-59.49646511
GCTACAC3150.09.3463843
CTAGGAC4100.09.2654953
TGTCCCG1252.7686523E-59.11660611
TATCGCG1054.509118E-49.04488857
ACTAGAC3800.08.9972843
TACACCG950.00182983378.9972845
CTGCCGG950.00183061488.9968619
CGCGATC1502.685665E-68.87127217
GTAGGAC2800.08.8187673
GTCCAGA5250.08.685741
TCTGTCG2304.0745363E-108.6708898
TAGGACC3100.08.5780554
Produced by FastQC (version 0.11.5)