##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294645_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4267356 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0834699518859 31.0 30.0 33.0 25.0 34.0 2 30.17524246863866 31.0 30.0 34.0 25.0 34.0 3 30.115460720877284 31.0 30.0 34.0 25.0 34.0 4 33.79911706452426 35.0 35.0 37.0 28.0 37.0 5 34.08491018794776 35.0 35.0 37.0 28.0 37.0 6 34.228136110509645 37.0 35.0 37.0 30.0 37.0 7 34.11187629998528 37.0 35.0 37.0 29.0 37.0 8 33.960303522837094 37.0 35.0 37.0 28.0 37.0 9 35.183945750014765 39.0 35.0 39.0 27.0 39.0 10 35.24542105228624 38.0 35.0 39.0 28.0 39.0 11 35.5129283800086 39.0 35.0 39.0 29.0 39.0 12 35.28201631173963 39.0 35.0 39.0 27.0 39.0 13 35.15360471448832 38.0 35.0 39.0 27.0 39.0 14 36.05097043696378 39.0 35.0 41.0 27.0 41.0 15 36.03862157270216 39.0 35.0 41.0 27.0 41.0 16 36.140331858884046 39.0 35.0 41.0 27.0 41.0 17 36.10841888982311 39.0 35.0 41.0 27.0 41.0 18 35.996111643837544 39.0 35.0 41.0 27.0 41.0 19 36.2653765001092 39.0 35.0 41.0 27.0 41.0 20 36.384671913943905 39.0 36.0 41.0 27.0 41.0 21 36.41734436967528 39.0 36.0 41.0 27.0 41.0 22 36.403726569801066 39.0 36.0 41.0 27.0 41.0 23 36.36474107152063 39.0 36.0 41.0 27.0 41.0 24 36.38470073741211 39.0 36.0 41.0 27.0 41.0 25 35.12299700329665 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.99575527340075 1101 2 0.9135204092655549 1101 3 1.2392656575355723 1101 4 1.0382933244056574 1101 5 0.7336486438839316 1101 6 0.7598566338756356 1101 7 0.7658793184291071 1101 8 0.8994993111977507 1101 9 1.1509705925189877 1101 10 1.0664511368155303 1101 11 0.8742243105435676 1101 12 1.0812412326605738 1101 13 1.092830073045782 1101 14 1.2074705745813503 1101 15 1.4106198225673126 1101 16 1.2743469628266908 1101 17 1.2324210551398878 1101 18 1.1540180761684766 1101 19 1.1770319586456566 1101 20 1.1237640235801152 1101 21 1.0313430483185542 1101 22 0.8677420355037881 1101 23 0.9697004369857041 1101 24 0.9909040458572278 1101 25 1.0395401992132776 1102 1 0.8955689325048013 1102 2 0.8312742514578986 1102 3 1.1380994886354756 1102 4 0.9456970994516993 1102 5 0.7363346172022602 1102 6 0.7019538999698014 1102 7 0.6581357271765782 1102 8 0.731896847012564 1102 9 1.0767428492624518 1102 10 1.004273193592077 1102 11 0.69555557322375 1102 12 0.9911664560256597 1102 13 0.9675068196238783 1102 14 1.157833065464672 1102 15 1.2162914978855284 1102 16 1.1145359314535312 1102 17 1.1426981434048642 1102 18 1.216758903179695 1102 19 0.9506323557856291 1102 20 0.36994884529166683 1102 21 0.5154393718313628 1102 22 0.6544002116920993 1102 23 0.7894188149981147 1102 24 0.8662377554772576 1102 25 0.8938467255833089 1103 1 0.702817982809492 1103 2 0.7007830937332784 1103 3 1.0274289331563722 1103 4 0.8052957149488265 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-1.2982375722091675 2207 10 -0.17289443519398873 2207 11 0.11386179167479327 2207 12 0.3216005651336573 2207 13 0.5569877681475575 2207 14 0.7396242501478127 2207 15 0.6308128640818254 2207 16 0.604834956654642 2207 17 0.5622253614077266 2207 18 0.6353375513617578 2207 19 -0.491545932591535 2207 20 0.10999047487856473 2207 21 -0.4911800967510871 2207 22 0.049535719744071116 2207 23 -0.6060008282492362 2207 24 -1.6857953049692327 2207 25 -0.35652390117438415 2208 1 0.45153406345364644 2208 2 0.09575819469155888 2208 3 0.5903329271971636 2208 4 0.5178949118751461 2208 5 0.40120237911314405 2208 6 0.3345073787406676 2208 7 0.3557672135592398 2208 8 0.5137740220817761 2208 9 -0.056112008407069425 2208 10 0.10568319444242746 2208 11 0.2730870937259766 2208 12 0.3209286509198819 2208 13 0.599302662692466 2208 14 0.8647627544829248 2208 15 0.768456887231487 2208 16 0.5087091461427917 2208 17 0.6131107875188562 2208 18 0.7646006669140846 2208 19 -1.3061377815598476 2208 20 -0.020339930259233086 2208 21 -0.16798833363137078 2208 22 0.27112213010182273 2208 23 0.40855640671421867 2208 24 0.5724972915989426 2208 25 0.6011584186330268 2209 1 0.17633706453016984 2209 2 -1.2698601530048386 2209 3 -0.2362175829438904 2209 4 0.15964593054230392 2209 5 0.14386373009704556 2209 6 0.23899923722842686 2209 7 0.18555854537352445 2209 8 0.3550529425892819 2209 9 -2.041937409339212 2209 10 -0.3610887603030122 2209 11 -0.07644921584362407 2209 12 0.3099872815954683 2209 13 0.40567275794787605 2209 14 0.5878874729072407 2209 15 0.8437383297247862 2209 16 0.6975797100666767 2209 17 0.5195560362194911 2209 18 0.7075844751259552 2209 19 0.4423440489210222 2209 20 0.33694108002683265 2209 21 0.4150931840486649 2209 22 0.4741842084458767 2209 23 0.4872691350839631 2209 24 0.5203481261618066 2209 25 0.6182274742269342 2210 1 0.04290240618449559 2210 2 0.06023556894895421 2210 3 0.08312453244200313 2210 4 0.16819708638189468 2210 5 0.10330535773447025 2210 6 -0.010071106901229143 2210 7 -0.027241634167602058 2210 8 0.09239451186644487 2210 9 0.21297510427074684 2210 10 -1.541455259490526 2210 11 -0.4700838632027313 2210 12 -2.488448575010139 2210 13 -0.8494891332410006 2210 14 -0.6371110397479995 2210 15 -0.8644772663811935 2210 16 -0.24011619268921436 2210 17 -0.02235483054601417 2210 18 0.25369571080661757 2210 19 0.11838388735815641 2210 20 0.16677032748270193 2210 21 0.19216575285705773 2210 22 0.14101181911824767 2210 23 0.046622776301795454 2210 24 0.07453307833771561 2210 25 -0.0855339485640485 2211 1 -0.30664229614745153 2211 2 -0.07495978073609777 2211 3 -1.541764517419736 2211 4 -0.6036794214975032 2211 5 -0.39480375405680945 2211 6 -0.4659380310715022 2211 7 -0.4704588724533494 2211 8 -0.10786283288378229 2211 9 0.09081784041731567 2211 10 0.07632845416102185 2211 11 -0.20643988536773605 2211 12 -0.2286797802056455 2211 13 -0.28653986709053214 2211 14 -0.868435376340635 2211 15 -0.425917491510198 2211 16 -0.13150145803429325 2211 17 -0.11828747294046593 2211 18 -0.06157448330996118 2211 19 -0.07744117063376876 2211 20 -0.11170600097021577 2211 21 -0.27286860147568603 2211 22 -0.3173575444657999 2211 23 -0.5992401089855903 2211 24 -0.3553918270344738 2211 25 -0.1367512726313791 2212 1 -0.2693364174097397 2212 2 -0.302617830926625 2212 3 -2.214451050512551 2212 4 -1.2218468064940993 2212 5 -0.5314639023773395 2212 6 -0.3654312658244834 2212 7 -0.19193449035832089 2212 8 -0.27116362635049995 2212 9 -0.8689214328558634 2212 10 -0.14028795140188777 2212 11 -0.21942335087688036 2212 12 0.09789436453885969 2212 13 -0.2590760630555238 2212 14 0.03693941051142957 2212 15 0.1410589688425148 2212 16 -0.9394865401003898 2212 17 -1.1612827169299678 2212 18 -0.17173009913121007 2212 19 -0.07705135121298667 2212 20 -0.10629441351738222 2212 21 0.02627769323008522 2212 22 -0.3045109085479112 2212 23 0.11632669768098225 2212 24 -0.045646974561407205 2212 25 0.06132963514829726 2213 1 -3.0357950522832695 2213 2 -1.353156876226322 2213 3 -2.306366533468477 2213 4 -0.993833273555019 2213 5 -0.7489633918073935 2213 6 -0.6654183973939851 2213 7 -0.46745756683502293 2213 8 -0.1258728421207067 2213 9 -0.3654201356416138 2213 10 -0.25641713941489286 2213 11 -0.5444893834404354 2213 12 -0.23991034889745322 2213 13 -0.6590625084340616 2213 14 -0.18726980453812558 2213 15 -0.31224117728795875 2213 16 -1.5576319388892017 2213 17 -1.0371473880405233 2213 18 -0.3403851529401791 2213 19 -0.19914294335586646 2213 20 -0.22955586725084487 2213 21 -0.03797976637065403 2213 22 -0.11004014445015287 2213 23 -0.003392177715319633 2213 24 -0.006605967476218666 2213 25 -0.04431829925710673 2214 1 -1.0007294205853299 2214 2 -0.710330390671551 2214 3 -6.554617295922398 2214 4 -2.082104912405569 2214 5 -1.2590832174406543 2214 6 -0.7433620952173001 2214 7 -0.6015931840583519 2214 8 -0.4205264701957958 2214 9 -0.3712557599610875 2214 10 -0.3444243896897703 2214 11 -0.9171878103093292 2214 12 -0.6943845818844494 2214 13 -5.170415193637687 2214 14 -4.015009583611523 2214 15 -2.228187928742095 2214 16 -2.840437868518343 2214 17 -3.430205070531592 2214 18 -1.203883200737664 2214 19 -0.9372298154139216 2214 20 -0.4948616004007462 2214 21 -0.4706499536401765 2214 22 -0.4248351841694813 2214 23 -0.3336579549986567 2214 24 -0.16321861951873018 2214 25 -0.12210233384966784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1682.0 3 218.0 4 230.0 5 484.0 6 55.0 7 124.0 8 363.0 9 883.0 10 1694.0 11 3194.0 12 5837.0 13 9455.0 14 13493.0 15 18207.0 16 22354.0 17 25117.0 18 26973.0 19 27981.0 20 28941.0 21 29915.0 22 30883.0 23 32856.0 24 35897.0 25 38761.0 26 43631.0 27 49263.0 28 57423.0 29 70096.0 30 86700.0 31 108514.0 32 140909.0 33 187425.0 34 251272.0 35 342517.0 36 481094.0 37 727502.0 38 1365413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11388079335977 21.730553058608713 12.996717792044258 23.15884835598726 2 14.254426225510711 21.958195788629105 41.7024992162912 22.08487876956898 3 18.07051476996659 27.94580425934215 29.833426224730275 24.150254745960982 4 11.495622199176983 17.525810420110847 39.779547254059224 31.199020126652943 5 12.540270952794156 38.70796739450775 35.49663073297648 13.255130919721624 6 29.78027631839368 39.78049675027132 18.041762929736635 12.397464001598365 7 24.86766370095162 33.51380720053915 24.732572105520113 16.88595699298912 8 23.358889030621814 38.35471989405047 22.025750163201295 16.26064091212642 9 24.181871903161735 17.085167302546882 22.764191228370496 35.96876956592088 10 14.254131001753562 30.560103478469262 34.25097937324802 20.934786146529152 11 31.899621406634036 26.95999328445807 23.22224551402268 17.918139794885217 12 19.989706168560904 28.21206230699066 33.11002675458239 18.68820476986604 13 25.830074781765365 24.712850401765575 27.640646088940873 21.816428727528187 14 19.49036111438748 23.31590046215907 29.323382144278327 27.870356279175123 15 22.59827639211552 30.82913601288051 24.61531174059258 21.95727585441139 16 21.048715660975297 32.2138419493299 26.414250189640637 20.323192200054166 17 19.292257358913893 30.923219856639296 29.78022215093582 20.004300633510987 18 19.694801807271183 30.693337207018345 30.198527139223387 19.41333384648708 19 20.172356096916946 29.302576664788287 30.40824180037525 20.116825437919513 20 21.731141396526095 28.96510033089157 29.139937517279122 20.16382075530321 21 21.867560268784086 29.89879444845542 28.382739460129198 19.850905822631297 22 20.20983724036499 29.44141891283467 30.105819088425985 20.242924758374357 23 20.151032911001778 29.929902701394607 29.583390882075065 20.335673505528547 24 20.268159589946407 28.936512612018493 29.63678943946897 21.158538358566126 25 21.08213129093608 28.835861706693056 28.54066463896517 21.541342363405693 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3609.0 1 3609.0 2 3460.0 3 3311.0 4 3311.0 5 3311.0 6 5423.5 7 7536.0 8 7536.0 9 7536.0 10 12366.0 11 17196.0 12 17196.0 13 17196.0 14 29441.5 15 41687.0 16 41687.0 17 41687.0 18 67238.0 19 92789.0 20 92789.0 21 92789.0 22 133859.0 23 174929.0 24 174929.0 25 174929.0 26 228879.5 27 282830.0 28 282830.0 29 282830.0 30 340177.0 31 397524.0 32 397524.0 33 397524.0 34 444696.0 35 491868.0 36 491868.0 37 491868.0 38 521529.5 39 551191.0 40 551191.0 41 551191.0 42 537825.5 43 524460.0 44 524460.0 45 524460.0 46 518778.5 47 513097.0 48 513097.0 49 513097.0 50 465032.0 51 416967.0 52 416967.0 53 416967.0 54 360295.0 55 303623.0 56 303623.0 57 303623.0 58 254833.0 59 206043.0 60 206043.0 61 206043.0 62 167005.5 63 127968.0 64 127968.0 65 127968.0 66 96222.0 67 64476.0 68 64476.0 69 64476.0 70 46521.0 71 28566.0 72 28566.0 73 28566.0 74 19930.5 75 11295.0 76 11295.0 77 11295.0 78 7701.0 79 4107.0 80 4107.0 81 4107.0 82 2799.0 83 1491.0 84 1491.0 85 1491.0 86 1008.0 87 525.0 88 525.0 89 525.0 90 361.5 91 198.0 92 198.0 93 198.0 94 120.0 95 42.0 96 42.0 97 42.0 98 35.0 99 28.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10245219756683062 2 0.055748805583597905 3 0.059029525542279575 4 0.05424904788820056 5 0.051202665069424724 6 0.07030114197175019 7 0.06659861516123801 8 0.06289608835072584 9 0.06856704713644703 10 0.06781716828874836 11 0.06144319808330966 12 0.062474281498895334 13 0.0628023534947635 14 0.06577843517156759 15 0.06863734827841877 16 0.06352879862847159 17 0.06720789172499318 18 0.1435314981923233 19 0.07135565910132645 20 0.06601277231147343 21 0.06254458264086708 22 0.06814524028461652 23 0.0669032534431156 24 0.07798740016066154 25 0.07231644137494037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4267356.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.07760814103393 #Duplication Level Percentage of deduplicated Percentage of total 1 90.3794607676088 72.37371043346529 2 7.324622477690054 11.730764970989467 3 1.19494174693636 2.870642308875971 4 0.3850493736291434 1.2333533142570046 5 0.17796023644791936 0.7125315039481116 6 0.1136579336311781 0.5460873282862274 7 0.0752386765671474 0.4217453281435816 8 0.05373678880294172 0.3442490813215574 9 0.03716502124512911 0.26784774070385853 >10 0.2260898326862327 3.3454063935916807 >50 0.017240523150310355 0.934932706291893 >100 0.012803397801322895 1.9767149155520385 >500 8.545436047838527E-4 0.4685789513262151 >1k 0.0010018781688580483 1.5620157078164663 >5k 1.1786801986558258E-4 0.5690053443248829 >10k+ 5.893400993279129E-5 0.6424139711057867 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 13869 0.3250021793354011 No Hit CCCATGTACTCTGCGTTGATACCAC 13356 0.3129806840582318 No Hit GTATCAACGCAGAGTACTTTTTTTT 6495 0.15220197236883917 No Hit ATTCCATTCCATTCCATTCCATTCC 6362 0.14908528840809157 No Hit GAGTACATGGGAAGCAGTGGTATCA 6204 0.1453827615975794 No Hit CATGTACTCTGCGTTGATACCACTG 5053 0.1184105567944179 No Hit GAATGGAATGGAATGGAATGGAATG 4859 0.11386441628024471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 2.3433713990583396E-5 3 0.0 0.0 0.0 0.0 2.3433713990583396E-5 4 0.0 0.0 0.0 2.3433713990583396E-5 2.3433713990583396E-5 5 0.0 4.686742798116679E-5 0.0 2.3433713990583396E-5 2.3433713990583396E-5 6 0.0 4.686742798116679E-5 0.0 2.3433713990583396E-5 2.3433713990583396E-5 7 0.0 4.686742798116679E-5 0.0 2.3433713990583396E-5 2.3433713990583396E-5 8 0.0 4.686742798116679E-5 0.0 2.3433713990583396E-5 2.3433713990583396E-5 9 0.0 4.686742798116679E-5 0.0 7.030114197175018E-5 2.3433713990583396E-5 10 0.0 4.686742798116679E-5 0.0 7.030114197175018E-5 2.3433713990583396E-5 11 0.0 4.686742798116679E-5 0.0 9.373485596233359E-5 2.3433713990583396E-5 12 0.0 4.686742798116679E-5 0.0 9.373485596233359E-5 2.3433713990583396E-5 13 0.0 4.686742798116679E-5 0.0 9.373485596233359E-5 2.3433713990583396E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAACGT 90 7.4327345E-6 11.616188 14 CTAGACT 515 0.0 10.14257 4 TCGCGCG 105 4.1245446E-5 9.948911 9 TAGACTG 715 0.0 9.829202 5 TATACTG 1010 0.0 9.685195 5 ACACCGT 130 4.2595148E-6 9.497133 6 AGGACCG 90 0.0011174321 9.497133 5 GCGCGCC 120 1.7095601E-5 9.496465 11 GCTACAC 315 0.0 9.346384 3 CTAGGAC 410 0.0 9.265495 3 TGTCCCG 125 2.7686523E-5 9.116606 11 TATCGCG 105 4.509118E-4 9.0448885 7 ACTAGAC 380 0.0 8.997284 3 TACACCG 95 0.0018298337 8.997284 5 CTGCCGG 95 0.0018306148 8.996861 9 CGCGATC 150 2.685665E-6 8.871272 17 GTAGGAC 280 0.0 8.818767 3 GTCCAGA 525 0.0 8.68574 1 TCTGTCG 230 4.0745363E-10 8.670889 8 TAGGACC 310 0.0 8.578055 4 >>END_MODULE