FastQCFastQC Report
Wed 25 May 2016
SRR1294645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267356
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT165770.388460676821901No Hit
CCCATGTACTCTGCGTTGATACCAC150150.3518572155686097No Hit
GTACATGGGAAGCAGTGGTATCAAC134680.3156052600251772No Hit
GGTATCAACGCAGAGTACTTTTTTT112640.2639573543899314No Hit
TATCAACGCAGAGTACTTTTTTTTT98970.23192346736480388No Hit
GAGTACATGGGAAGCAGTGGTATCA65470.1534205254963495No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64260.1505850461034889No Hit
ATTCCATTCCATTCCATTCCATTCC62160.1456639661654664No Hit
CATGTACTCTGCGTTGATACCACTG56250.1318146411970316No Hit
GAATGGAATGGAATGGAATGGAATG54760.1283230178124347No Hit
GCGTTGATACCACTGCTTCCCATGT45760.10723267522090962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTA600.005958523611.06321713
GCTAGAC1605.4205884E-1010.6907763
TCGGAAC1002.4496781E-510.43103413
CTAGTAC2950.010.3082433
GTCTTGG6100.09.9801121
ACACCGT1151.0035039E-59.9327616
ACAGCGT1252.5084028E-69.8996528
CCTACAC3950.09.8637823
GTATAGG4550.09.8258871
TATTCCG2350.09.7214255
TAGACAG6650.09.59046655
TTAGACT5100.09.5188964
CGTGCAA900.00109428189.51889510
TATATCG1106.695067E-59.5188955
TCGCTCT2151.0186341E-109.2975254
CAGGACT6300.09.2167084
TAGACTG5700.09.1848995
ATTAGAC3850.09.13266853
ACGGTAC1151.1077925E-49.0897423
GTACTAG2950.09.0286191
Produced by FastQC (version 0.11.5)