##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4267356 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.328974662531085 31.0 31.0 34.0 28.0 34.0 2 31.465668202980957 31.0 31.0 34.0 28.0 34.0 3 31.575264871269237 31.0 31.0 34.0 28.0 34.0 4 35.17915074345801 37.0 35.0 37.0 32.0 37.0 5 35.28376048307195 37.0 35.0 37.0 32.0 37.0 6 34.88388571283952 37.0 35.0 37.0 32.0 37.0 7 34.73996779270349 37.0 35.0 37.0 32.0 37.0 8 34.725871710726736 37.0 35.0 37.0 32.0 37.0 9 36.21482693264869 39.0 35.0 39.0 31.0 39.0 10 35.9601908066728 39.0 35.0 39.0 30.0 39.0 11 36.151811801030895 39.0 35.0 39.0 31.0 39.0 12 36.085639679464286 39.0 35.0 39.0 30.0 39.0 13 35.961189082888794 39.0 35.0 39.0 30.0 39.0 14 37.022989645110464 40.0 36.0 41.0 30.0 41.0 15 37.08196269540203 40.0 36.0 41.0 31.0 41.0 16 37.11544361426607 40.0 36.0 41.0 31.0 41.0 17 37.14671379655224 40.0 36.0 41.0 31.0 41.0 18 37.15159152411938 40.0 36.0 41.0 31.0 41.0 19 37.16856995291698 40.0 37.0 41.0 30.0 41.0 20 37.23370419529095 40.0 37.0 41.0 31.0 41.0 21 37.110734843776804 40.0 36.0 41.0 30.0 41.0 22 37.07330487543106 40.0 36.0 41.0 30.0 41.0 23 37.093433967074695 40.0 36.0 41.0 30.0 41.0 24 37.01650014669505 40.0 36.0 41.0 30.0 41.0 25 35.906893870584035 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5037622100858634 1101 2 0.4973304436592656 1101 3 0.38974256065070634 1101 4 0.33637349142878037 1101 5 0.35990516765497205 1101 6 0.4345345986909379 1101 7 0.44279238931466125 1101 8 0.43393070890098073 1101 9 0.5648469791972559 1101 10 0.5753378172806691 1101 11 0.5982595667141766 1101 12 0.6289814731676557 1101 13 0.6272177492429591 1101 14 0.840385614457638 1101 15 0.8458377331635418 1101 16 0.881910537994429 1101 17 0.849687197554637 1101 18 0.8605622268655253 1101 19 0.9767192639488229 1101 20 0.8711403675356735 1101 21 1.1686820297835823 1101 22 1.0453679597419026 1101 23 0.9484644179968242 1101 24 1.1378771668729115 1101 25 1.0020462617246082 1102 1 0.38644510725816517 1102 2 0.3767115319312708 1102 3 0.31501578501127625 1102 4 0.3258813655182351 1102 5 0.36236050581812407 1102 6 0.4323423671239084 1102 7 0.4813281826128062 1102 8 0.5199092936067018 1102 9 0.6252911869933158 1102 10 0.7311910165945719 1102 11 0.6994479592988725 1102 12 0.7069798601768724 1102 13 0.7504355738386366 1102 14 0.9060468701118864 1102 15 0.8560728465579217 1102 16 0.8849328833029162 1102 17 0.8025860384500163 1102 18 0.7328324182388855 1102 19 0.953817135433745 1102 20 0.8179075028416349 1102 21 1.1083929481570394 1102 22 1.074268330463589 1102 23 0.8992128066394685 1102 24 1.0500170274024683 1102 25 0.9815296168442771 1103 1 0.3463872018090619 1103 2 0.33168827585451766 1103 3 0.2788626909378955 1103 4 0.22887576150369426 1103 5 0.28164553767437184 1103 6 0.3181742743437468 1103 7 0.3342922794947043 1103 8 0.3378616035388404 1103 9 0.3345825031906884 1103 10 0.49376203697217846 1103 11 0.5555084751322781 1103 12 0.6595364227783236 1103 13 0.703131933849825 1103 14 0.7937125143185568 1103 15 0.7239925898790531 1103 16 0.7284516742114491 1103 17 0.7420232341811825 1103 18 0.7287854899048298 1103 19 0.8787371091484957 1103 20 0.759344759089231 1103 21 1.001091403196412 1103 22 0.9160063920183106 1103 23 0.8575421755519983 1103 24 1.0692872423986017 1103 25 0.9155765830940297 1104 1 0.1561844919935318 1104 2 0.19658319328639706 1104 3 0.07411572306011749 1104 4 0.13841779634084617 1104 5 0.13349067414508653 1104 6 0.11949777845381249 1104 7 0.1326363738678893 1104 8 0.14100818916521263 1104 9 -0.06790923755947631 1104 10 0.3851527150584175 1104 11 0.4631709594960185 1104 12 0.40167069375441145 1104 13 -0.10460845019375142 1104 14 0.3547253830206287 1104 15 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5 0.1393332030218417 2207 6 0.30871540421045296 2207 7 0.23784015530210922 2207 8 0.2843677244197096 2207 9 0.36193085547633785 2207 10 0.525243652422354 2207 11 0.43554201358799105 2207 12 0.41749771689836734 2207 13 0.472074177081673 2207 14 0.4696682690849059 2207 15 0.43614130586662014 2207 16 0.5022298173128519 2207 17 0.48667744229162935 2207 18 0.5418564711596616 2207 19 0.6632230727215358 2207 20 0.5259084853601479 2207 21 0.8925023900907618 2207 22 0.7375579421786682 2207 23 0.5766755996746298 2207 24 0.7464223234387148 2207 25 0.7433884947120433 2208 1 0.1653831892240234 2208 2 0.17603226360487767 2208 3 0.18687216972630338 2208 4 0.12223215511069441 2208 5 0.11406581916418901 2208 6 0.22795822518653353 2208 7 0.23273960774906755 2208 8 0.20183096969792302 2208 9 0.3008083874084875 2208 10 0.3688743723050152 2208 11 -0.023654612286264864 2208 12 -0.19345238422130961 2208 13 0.0342092014231028 2208 14 -0.4715985088610495 2208 15 0.18780372108846421 2208 16 0.25481153625339914 2208 17 0.22422336799201048 2208 18 0.3038140803995333 2208 19 0.5032624129975289 2208 20 0.4358048052863239 2208 21 0.7078827372930903 2208 22 0.7119967991114677 2208 23 0.638217540965833 2208 24 0.6941528835998128 2208 25 0.6003701974718396 2209 1 0.011526805226878878 2209 2 0.022694661475942013 2209 3 0.09874438289703846 2209 4 -0.4800405623240067 2209 5 -0.04075334140146225 2209 6 0.0973258873616416 2209 7 0.06757251370337514 2209 8 0.19591532469522832 2209 9 0.23049479742131496 2209 10 0.32248568348123996 2209 11 0.29445703503263587 2209 12 0.33045057360119046 2209 13 0.34758315158075703 2209 14 0.38832392692304296 2209 15 -0.21923552952146963 2209 16 -0.20532447250263175 2209 17 0.16124711175238815 2209 18 -0.23806143925640555 2209 19 0.28626061923762336 2209 20 0.33124415325942635 2209 21 0.6022235197080477 2209 22 0.587566601744598 2209 23 0.49942774118966327 2209 24 0.6791316511698824 2209 25 0.6754613089536647 2210 1 -0.26042671529971884 2210 2 -0.21141279987036654 2210 3 -0.09483786699982133 2210 4 -0.1408857537803243 2210 5 -0.118488853472968 2210 6 -0.6301749663105269 2210 7 -0.28058605958322147 2210 8 -0.403732099139809 2210 9 -0.09552421148189438 2210 10 0.11090882236690902 2210 11 0.05286795119743459 2210 12 -0.012682377447092108 2210 13 0.06709771176368662 2210 14 -0.046845971445868884 2210 15 -0.06975536238349633 2210 16 0.009192159902006836 2210 17 -1.0042817836840214 2210 18 -1.500935684206759 2210 19 -0.35205847929529455 2210 20 -0.22811635623815363 2210 21 -0.4344190746665717 2210 22 -0.41760438119543863 2210 23 -0.31476038085832414 2210 24 0.09760684075016712 2210 25 0.09443285247679256 2211 1 -0.19394026938560316 2211 2 -0.17686236089502927 2211 3 -0.15000328844354982 2211 4 -0.06293745862971889 2211 5 -0.08584991411458986 2211 6 0.011564207636652668 2211 7 -0.0917841304507192 2211 8 -0.033151131452065385 2211 9 0.10826352198996148 2211 10 0.12044487505586687 2211 11 -0.2784515006105934 2211 12 -0.12251330432901142 2211 13 -0.03489111041943005 2211 14 -0.09543242200648905 2211 15 -0.18564961751936693 2211 16 -0.13534456593409772 2211 17 -0.2595534789783045 2211 18 -0.3572060201012306 2211 19 -0.2163508229315525 2211 20 -0.2603035125905748 2211 21 0.1110974674777978 2211 22 0.024678314395508494 2211 23 -0.6683523059519416 2211 24 0.012768139912246568 2211 25 -0.7226283896167018 2212 1 -0.11645454134501776 2212 2 -0.2566612717345329 2212 3 -0.10827152920474958 2212 4 -0.012119131488169899 2212 5 -0.0680371114868521 2212 6 -0.05678552910367074 2212 7 -0.12548789774157143 2212 8 -0.23897389411473569 2212 9 -0.016729287592141873 2212 10 -0.3841175223267115 2212 11 -0.09922960030993977 2212 12 -0.2225154932973865 2212 13 -0.06822386663798596 2212 14 -0.16735365514465883 2212 15 0.017808296589024053 2212 16 -0.26514166534506245 2212 17 -0.5134661074114462 2212 18 -0.30157493708586713 2212 19 -0.32030622777286055 2212 20 -0.4292782951409748 2212 21 0.05703356378752744 2212 22 -0.2893692414382514 2212 23 -0.15521099828053764 2212 24 -0.4105680934863898 2212 25 -0.057374510089744035 2213 1 -0.15829490989034767 2213 2 -0.1814769103962739 2213 3 -0.03214470494727806 2213 4 0.010388496041358053 2213 5 -0.023495187193077527 2213 6 -0.17764235351091173 2213 7 -0.30525525017933575 2213 8 -0.19960248138164616 2213 9 -0.24025580530084767 2213 10 -0.22783923849092957 2213 11 0.02395671707988356 2213 12 0.10669743564324108 2213 13 0.07996317610895431 2213 14 -0.618231249518324 2213 15 -0.11687096741571423 2213 16 -0.2341075824593375 2213 17 -0.16580314849461075 2213 18 -0.1227379530599535 2213 19 -0.17735941233497954 2213 20 -0.2890453962887207 2213 21 -0.05204203546868058 2213 22 -0.3396321810414733 2213 23 -0.4583296986010197 2213 24 -0.6662372998116055 2213 25 -0.4357146072606497 2214 1 -0.300700968013107 2214 2 -0.1629225932322278 2214 3 -0.1653089898276825 2214 4 -0.2041325028209684 2214 5 -0.14907003205077984 2214 6 -0.14018940361350474 2214 7 -0.18783448472672148 2214 8 -0.2129211035699896 2214 9 -0.3106140689889685 2214 10 -0.17185085632773678 2214 11 -0.08588471844706902 2214 12 -0.357636049532303 2214 13 -0.47868912124845764 2214 14 -0.23357944507583284 2214 15 -0.0675974065021876 2214 16 -0.4293360363164709 2214 17 -0.5812756791701688 2214 18 -0.34795905897485113 2214 19 -0.6336359131884279 2214 20 -0.588612531529769 2214 21 -0.6662933808746132 2214 22 -0.9422620652675135 2214 23 -1.251340340527321 2214 24 -0.6499711074420205 2214 25 -1.0689740796585596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 23.0 9 42.0 10 119.0 11 216.0 12 646.0 13 1573.0 14 3431.0 15 6074.0 16 8854.0 17 11531.0 18 13840.0 19 15610.0 20 17189.0 21 19084.0 22 21027.0 23 23510.0 24 26682.0 25 30133.0 26 35334.0 27 39886.0 28 47196.0 29 58659.0 30 75347.0 31 97138.0 32 130671.0 33 176507.0 34 240480.0 35 333840.0 36 474281.0 37 746603.0 38 1611824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.15943817143704 21.825171099968074 12.11289489863249 24.902495829962394 2 14.054815206418214 22.14211797656441 40.517781970850336 23.28528484616704 3 17.469136265978605 27.862069871758173 30.130471020988185 24.53832284127504 4 11.43261254483689 17.819869669815354 39.7401599070041 31.00735787834365 5 12.850466468264617 38.24531146832439 35.63893483554483 13.265287227866168 6 30.081960086087268 39.915787657965524 17.549268977717198 12.452983278230013 7 24.93445376417981 33.82832675566173 24.42025625964794 16.816963220510523 8 23.38786512703551 38.637446581709916 21.58217281685516 16.392515474399417 9 24.083757855286734 16.610335831449454 22.870709660728476 36.43519665253533 10 14.12477590028092 30.54275657947364 34.23719043652979 21.095277083715654 11 32.117829130174805 26.55270655270655 23.091601889955797 18.23786242716284 12 20.064537144008753 28.124485917357774 33.073247549392946 18.737729389240528 13 26.109164550602294 24.35053461675098 27.634043187397534 21.906257645249188 14 19.5631964706646 22.92340928812681 29.596826485200662 27.916567756007932 15 22.66982650198216 30.49208271907063 24.8103313773006 22.02775940164661 16 20.936337320271992 31.972879662347907 26.530229174961235 20.56055384241887 17 19.065655204606884 30.889703549318853 29.937169496601406 20.107471749472857 18 19.52672636191952 30.508717255077578 30.505834898125084 19.45872148487782 19 19.99763786945198 29.01570395005406 30.99715185171819 19.989506328775768 20 21.548353110257963 28.608102411170215 29.803013441372357 20.040531037199457 21 21.689653620937406 29.5740352087526 28.871327109952126 19.864984060357862 22 19.951200387509793 29.22382979002622 30.564623983565987 20.260345838898004 23 19.841789475065568 29.699542011064473 30.192189442070866 20.266479071799093 24 19.97220760869438 28.756548259987746 30.316765303097586 20.954478828220292 25 20.87429524729705 28.504336606224534 29.18113822936735 21.440229917111076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1579.0 1 1579.0 2 1936.5 3 2294.0 4 2294.0 5 2294.0 6 4971.5 7 7649.0 8 7649.0 9 7649.0 10 11901.5 11 16154.0 12 16154.0 13 16154.0 14 26624.0 15 37094.0 16 37094.0 17 37094.0 18 62269.0 19 87444.0 20 87444.0 21 87444.0 22 129909.0 23 172374.0 24 172374.0 25 172374.0 26 227682.5 27 282991.0 28 282991.0 29 282991.0 30 342303.5 31 401616.0 32 401616.0 33 401616.0 34 448786.5 35 495957.0 36 495957.0 37 495957.0 38 524274.0 39 552591.0 40 552591.0 41 552591.0 42 538330.0 43 524069.0 44 524069.0 45 524069.0 46 520355.0 47 516641.0 48 516641.0 49 516641.0 50 468995.5 51 421350.0 52 421350.0 53 421350.0 54 363414.0 55 305478.0 56 305478.0 57 305478.0 58 256421.0 59 207364.0 60 207364.0 61 207364.0 62 167817.0 63 128270.0 64 128270.0 65 128270.0 66 96167.0 67 64064.0 68 64064.0 69 64064.0 70 45792.0 71 27520.0 72 27520.0 73 27520.0 74 18828.5 75 10137.0 76 10137.0 77 10137.0 78 6769.5 79 3402.0 80 3402.0 81 3402.0 82 2200.0 83 998.0 84 998.0 85 998.0 86 609.5 87 221.0 88 221.0 89 221.0 90 148.5 91 76.0 92 76.0 93 76.0 94 46.5 95 17.0 96 17.0 97 17.0 98 11.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09853876733040318 2 0.0 3 0.13404084402613703 4 0.011740290709282281 5 0.021910522581195475 6 0.012255832417075115 7 0.021137210019506223 8 0.01858293519453263 9 1.6403599793408377E-4 10 0.39874807726376704 11 0.06341163005851867 12 0.0010779508435668362 13 0.0 14 8.670474176515858E-4 15 0.001054517129576253 16 1.1716856995291699E-4 17 4.686742798116679E-5 18 3.5150570985875094E-4 19 5.155417077928348E-4 20 2.1090342591525057E-4 21 1.8746971192466717E-4 22 0.00255427482497359 23 9.373485596233359E-5 24 2.3433713990583396E-5 25 0.0018512634052560884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4267356.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.404681935769 #Duplication Level Percentage of deduplicated Percentage of total 1 89.8667901233619 70.45977096210697 2 7.706286338647622 12.084178585752573 3 1.2908343589362499 3.036223720324769 4 0.38933422228325154 1.221025034593133 5 0.18906470830980718 0.7411779160154688 6 0.11584894393787176 0.5449859761226142 7 0.07568766016202987 0.4153986845026569 8 0.050174268778353286 0.31471180679412597 9 0.04067106347034279 0.286992161584353 >10 0.24013916007559022 3.4595367365538947 >50 0.018878019600232752 1.0121406525877075 >100 0.013888741600781993 2.039386236547476 >500 0.001051046300275099 0.5443096260831021 >1k 0.0010510457500751936 1.5678267368965448 >5k 1.8017927144142255E-4 0.9461961146083256 >10k+ 1.2011951429428169E-4 1.3261390489262532 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16577 0.388460676821901 No Hit CCCATGTACTCTGCGTTGATACCAC 15015 0.3518572155686097 No Hit GTACATGGGAAGCAGTGGTATCAAC 13468 0.3156052600251772 No Hit GGTATCAACGCAGAGTACTTTTTTT 11264 0.2639573543899314 No Hit TATCAACGCAGAGTACTTTTTTTTT 9897 0.23192346736480388 No Hit GAGTACATGGGAAGCAGTGGTATCA 6547 0.1534205254963495 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6426 0.1505850461034889 No Hit ATTCCATTCCATTCCATTCCATTCC 6216 0.1456639661654664 No Hit CATGTACTCTGCGTTGATACCACTG 5625 0.1318146411970316 No Hit GAATGGAATGGAATGGAATGGAATG 5476 0.1283230178124347 No Hit GCGTTGATACCACTGCTTCCCATGT 4576 0.10723267522090962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.3433713990583396E-5 0.0 4 0.0 0.0 0.0 2.3433713990583396E-5 0.0 5 0.0 2.3433713990583396E-5 0.0 2.3433713990583396E-5 0.0 6 0.0 2.3433713990583396E-5 0.0 2.3433713990583396E-5 0.0 7 0.0 2.3433713990583396E-5 0.0 2.3433713990583396E-5 0.0 8 0.0 2.3433713990583396E-5 0.0 2.3433713990583396E-5 0.0 9 0.0 2.3433713990583396E-5 0.0 2.3433713990583396E-5 0.0 10 0.0 2.3433713990583396E-5 0.0 2.3433713990583396E-5 0.0 11 0.0 2.3433713990583396E-5 0.0 7.030114197175018E-5 0.0 12 0.0 2.3433713990583396E-5 0.0 7.030114197175018E-5 0.0 13 0.0 2.3433713990583396E-5 0.0 7.030114197175018E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATTA 60 0.0059585236 11.063217 13 GCTAGAC 160 5.4205884E-10 10.690776 3 TCGGAAC 100 2.4496781E-5 10.431034 13 CTAGTAC 295 0.0 10.308243 3 GTCTTGG 610 0.0 9.980112 1 ACACCGT 115 1.0035039E-5 9.932761 6 ACAGCGT 125 2.5084028E-6 9.899652 8 CCTACAC 395 0.0 9.863782 3 GTATAGG 455 0.0 9.825887 1 TATTCCG 235 0.0 9.721425 5 TAGACAG 665 0.0 9.5904665 5 TTAGACT 510 0.0 9.518896 4 CGTGCAA 90 0.0010942818 9.518895 10 TATATCG 110 6.695067E-5 9.518895 5 TCGCTCT 215 1.0186341E-10 9.297525 4 CAGGACT 630 0.0 9.216708 4 TAGACTG 570 0.0 9.184899 5 ATTAGAC 385 0.0 9.1326685 3 ACGGTAC 115 1.1077925E-4 9.089742 3 GTACTAG 295 0.0 9.028619 1 >>END_MODULE