FastQCFastQC Report
Wed 25 May 2016
SRR1294644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298968
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62172.0794867678146156No Hit
GGTATCAACGCAGAGTACTTTTTTT40341.349308287174547No Hit
TATCAACGCAGAGTACTTTTTTTTT34941.168686949773889No Hit
GTACTGGTTCACTATCGGTCAGTCA21110.7060956356533141No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15890.5314950094993445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA14990.5013914532659014No Hit
ATACAGGGTGACAGCCCCGTACACA14910.4987155816007064No Hit
GGATACCACGTGTCCCGCCCTACTC13720.45891199058093174No Hit
GTTAATGATAGTGTGTCGAAACACA11250.37629445291803804No Hit
GGTATCAACGCAGAGTACATGGGGG10580.3538840277220305No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10520.35187712397313425No Hit
ACCCTGTATCGCGCGCCTTTCCAGA9880.33047015065157476No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8150.2726044258917342No Hit
ACTTAGATGTTTCAGTTCCCCCGGT8030.2685906183939418No Hit
GTTCACTATCGGTCAGTCAGGAGTA7980.266918198603195No Hit
GTATTTAGCCTTGGAGGATGGTCCC7450.24919054882127853No Hit
GCCTTGGAGGATGGTCCCCCCATAT7000.234138770704557No Hit
CCCATATTCAGACAGGATACCACGT6540.21875250862968612No Hit
AGCGTACACGGTGGATGCCCTGGCA6500.21741457279708865No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG6420.21473870113189372No Hit
CTATCGGTCAGTCAGGAGTATTTAG6280.2100559257178026No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6200.20738005405260765No Hit
GTACTTAGATGTTTCAGTTCCCCCG6190.2070455700944583No Hit
GTATCAACGCAGAGTACATGGGGGT6160.20604211822001015No Hit
ATCATTAACTGAATCCATAGGTTAA6110.20436969842926334No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6010.2010248588477697No Hit
GCCCAGAGCCTGAATCAGTGTGTGT5980.20002140697332155No Hit
GTGGATGCCCTGGCAGTCAGAGGCG5940.1986834711407241No Hit
GGGTTTCCCCATTCGGAAATCGCCG5800.19400069572663295No Hit
GTACATGGGGGTTAAGCGACTAAGC5660.1893179203125418No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5640.18864895239624307No Hit
TCATTAACCTATGGATTCAGTTAAT5630.1883144684380937No Hit
ATCAACGCAGAGTACTTTTTTTTTT5540.1853041128147494No Hit
TATTCAGACAGGATACCACGTGTCC5470.18296272510770384No Hit
GGTTAATGAGGCGAACCGGGGGAAC5390.18028685344250892No Hit
CACACACACTGATTCAGGCTCTGGG5210.1742661421958203No Hit
GTGGTATCAACGCAGAGTACATGGG5170.17292820636322281No Hit
GTGCTAATCTGCGATAAGCGTCGGT5120.17125578657247598No Hit
CGGTACTGGTTCACTATCGGTCAGT4880.16322817157689118No Hit
TCACGGTACTGGTTCACTATCGGTC4760.15921436407909875No Hit
GGTATCAACGCAGAGTACATGGGGA4730.15821091220465067No Hit
AGCTCACAGCATGTGCATTTTTGTG4690.1568729763720532No Hit
GGGTACTTAGATGTTTCAGTTCCCC4590.15352813679055952No Hit
CCTATGGATTCAGTTAATGATAGTG4570.1528591688742608No Hit
CATCTAAGTACCCCGAGGAAAAGAA4560.15252468491611143No Hit
GGTTCACTATCGGTCAGTCAGGAGT4440.148510877418319No Hit
ACGCAGAGTACATGGGGGTTAAGCG4390.1468384576275722No Hit
GTGGTATCAACGCAGAGTACTTTTT4300.14382810200422788No Hit
GTTATAACCGGCGATTTCCGAATGG4250.14215568221348104No Hit
GGGTGACAGCCCCGTACACAAAAAT4230.14148671429718232No Hit
ATGATAGTGTGTCGAAACACACTGG4090.13680393888309117No Hit
CCCCAGTAGCGGCGAGCGAACGGGG4050.1354660030504937No Hit
TTCCAGACGCTTCCACTAACACACA3990.1334590993015975No Hit
CTCTACTTGTGCGCTATCGGTCTCT3980.13312461534344813No Hit
GTCCCGCCCTACTCATCGAGCTCAC3950.13212116346900002No Hit
GCCCTACTCATCGAGCTCACAGCAT3890.13011425972010382No Hit
GGAGTATTTAGCCTTGGAGGATGGT3840.12844183992935698No Hit
GATATGAACCGTTATAACCGGCGAT3820.12777287201305826No Hit
TAATGATAGTGTGTCGAAACACACT3690.12342458055711648No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC3600.12041422493377217No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3530.11807283722672661No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3520.11773835326857723No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA3460.11573144951968105No Hit
GGTGATATGAACCGTTATAACCGGC3380.11305557785448611No Hit
GTATCAACGCAGAGTACATGGGGGG3330.11138315806373926No Hit
CTACTGGGGGAATCTCGGTTGATTT3320.11104867410558988No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3140.1050279628589013No Hit
GGTATCAACGCAGAGTACATGGGCA3100.10369002702630382No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3070.10268657515185571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA555.8801015E-717.2699892
TCTCTCG350.002164556916.28585819
GGTATCA9850.015.9111831
TTGTGCG601.4595644E-615.8308237
TGCGCTA601.4621164E-615.82817310
ACTTGTG551.1239219E-515.5429915
TGTGCGC551.1257071E-515.5403898
GTGCGCT551.1257071E-515.5403899
GTACAAA807.6470315E-915.4350521
CTACTTG508.688195E-515.197593
GCGCTAT653.3615015E-614.61062111
AATCGCC1101.8189894E-1013.82524618
ATCGCCG1101.8371793E-1013.81830419
GTACAAG551.9544513E-413.8159911
TCTACTT551.9544513E-413.8159912
ATCATAA551.9544513E-413.8159911
CTCTACT551.9544513E-413.8159911
TACAAGA500.001496395313.2978922
ACAGGGT1950.013.1517613
TGGGCAT957.323797E-812.9979376
Produced by FastQC (version 0.11.5)