FastQCFastQC Report
Wed 25 May 2016
SRR1294644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298968
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT165105.522330149046051No Hit
GGTATCAACGCAGAGTACTTTTTTT119854.008790238420165No Hit
TATCAACGCAGAGTACTTTTTTTTT103613.4655882903855932No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58021.9406759251826282No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35501.1874180514302535No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28630.9576275721816382No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21650.72415776939338No Hit
GTACTGGTTCACTATCGGTCAGTCA20260.6776644992106179No Hit
ATACAGGGTGACAGCCCCGTACACA17330.5796606994728534No Hit
ATCAACGCAGAGTACTTTTTTTTTT17040.5699606646865216No Hit
GGATACCACGTGTCCCGCCCTACTC13910.4652671857857697No Hit
GTGGTATCAACGCAGAGTACTTTTT12270.4104118166492735No Hit
GTTAATGATAGTGTGTCGAAACACA11630.389004843327714No Hit
ACCCTGTATCGCGCGCCTTTCCAGA11300.37796687270878493No Hit
GGTATCAACGCAGAGTACATGGGGG10730.35890128709427094No Hit
ACTTAGATGTTTCAGTTCCCCCGGT8630.2886596558829039No Hit
AGCGTACACGGTGGATGCCCTGGCA7690.25721816381686335No Hit
GTTCACTATCGGTCAGTCAGGAGTA7360.24618019319793422No Hit
CAACGCAGAGTACTTTTTTTTTTTT7320.24484225736533674No Hit
CCCATATTCAGACAGGATACCACGT7290.24383880549088865No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG6890.23045944716491396No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6710.22443873591822533No Hit
GTATCAACGCAGAGTACATGGGGGT6460.21607663696449117No Hit
GCCTTGGAGGATGGTCCCCCCATAT6370.21306628134114686No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6340.21206282946669877No Hit
AACGCAGAGTACTTTTTTTTTTTTT6260.20938695780150385No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6110.20436969842926334No Hit
CACACACACTGATTCAGGCTCTGGG6050.2023627946803671No Hit
CTATCGGTCAGTCAGGAGTATTTAG6020.20135934280591902No Hit
GTATTTAGCCTTGGAGGATGGTCCC5910.19768001926627599No Hit
GTACTTAGATGTTTCAGTTCCCCCG5850.19567311551737981No Hit
TCATTAACCTATGGATTCAGTTAAT5830.19500414760108103No Hit
ATCATTAACTGAATCCATAGGTTAA5790.1936662117684836No Hit
GGTTAATGAGGCGAACCGGGGGAAC5730.1916593080195874No Hit
CGGTACTGGTTCACTATCGGTCAGT5710.19099034010328864No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5710.19099034010328864No Hit
GTGGATGCCCTGGCAGTCAGAGGCG5680.18998688822884055No Hit
GGGTTTCCCCATTCGGAAATCGCCG5570.1863075646891975No Hit
GTGCTAATCTGCGATAAGCGTCGGT5550.1856385967728988No Hit
ACGCAGAGTACATGGGGGTTAAGCG5410.1809558213588076No Hit
GTACATGGGGGTTAAGCGACTAAGC5410.1809558213588076No Hit
TCAACGCAGAGTACTTTTTTTTTTT5390.18028685344250892No Hit
CATCTAAGTACCCCGAGGAAAAGAA5360.1792834015680608No Hit
CCTATGGATTCAGTTAATGATAGTG5290.17694201386101524No Hit
TCACGGTACTGGTTCACTATCGGTC5280.17660752990286585No Hit
GGTATCAACGCAGAGTACATGGGGA4950.16556955928393674No Hit
TATTCAGACAGGATACCACGTGTCC4830.16155575178614434No Hit
GTGGTATCAACGCAGAGTACATGGG4820.16122126782799495No Hit
AGCTCACAGCATGTGCATTTTTGTG4810.16088678386984562No Hit
GGGTACTTAGATGTTTCAGTTCCCC4580.15319365283241015No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4550.15219020095796207No Hit
CCCCAGTAGCGGCGAGCGAACGGGG4480.1498488132509165No Hit
GGGTGACAGCCCCGTACACAAAAAT4340.14516603783682533No Hit
CTCTACTTGTGCGCTATCGGTCTCT4310.14416258596237724No Hit
GGTTCACTATCGGTCAGTCAGGAGT4220.14115223033903296No Hit
ATGATAGTGTGTCGAAACACACTGG4200.1404832624227342No Hit
GTTATAACCGGCGATTTCCGAATGG4130.13814187471568865No Hit
GATATGAACCGTTATAACCGGCGAT3900.13044874367825318No Hit
GCCCTACTCATCGAGCTCACAGCAT3880.12977977576195446No Hit
GTCCCGCCCTACTCATCGAGCTCAC3870.1294452918038051No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3760.12576596826416206No Hit
GGAGTATTTAGCCTTGGAGGATGGT3680.12309009659896711No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA3610.12074870889192155No Hit
GGTGATATGAACCGTTATAACCGGC3610.12074870889192155No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3600.12041422493377217No Hit
GTATCAACGCAGAGTACATGGGGGG3430.11472799764523293No Hit
CAGTAGCGGCGAGCGAACGGGGAGC3390.11339006181263547No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC3390.11339006181263547No Hit
GGTATCAACGCAGAGTACATGGGCA3350.112052125980038No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA3320.11104867410558988No Hit
CACTATCATTAACTGAATCCATAGG3310.11071419014744054No Hit
TCGGTAAGGTGATATGAACCGTTAT3290.1100452222311418No Hit
TTCCAGACGCTTCCACTAACACACA3250.10870728639854432No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3240.10837280244039495No Hit
TAATGATAGTGTGTCGAAACACACT3160.10569693077520001No Hit
CATTAACCTATGGATTCAGTTAATG3040.10168312327740762No Hit
CTGTCACCCTGTATCGCGCGCCTTT3020.10101415536110889No Hit
CTACTGGGGGAATCTCGGTTGATTT3010.10067967140295951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAGA250.00602674718.9968173
GTACAAA453.5082834E-516.897381
TACATAA402.75527E-416.6222152
GCTTGGT402.7871295E-416.59719515
GGTATCA19650.015.865481
GAATATA456.714054E-414.7852091
CTCTACT653.3326742E-614.6227341
TCTACTT653.3559536E-614.6129362
TGTGCGC602.5025493E-514.2811038
ACTTGTG707.0548176E-613.6010515
GTACATA1051.3405952E-913.5782521
GCTAAAA500.001488948213.3066871
CCGAACA500.001513584313.27775617
CAGTAGT500.001513584313.27775618
CTTGTGC655.3138487E-513.1825576
TTGTGCG655.3138487E-513.1825577
ATCGCCG957.4704076E-812.97825619
AATCGCC957.4704076E-812.97825618
GTGCGCT751.4415085E-512.6900539
CCGCTAC604.0575833E-412.6730361
Produced by FastQC (version 0.11.5)