FastQCFastQC Report
Wed 25 May 2016
SRR1294643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410350
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC122760.2783452560454386No Hit
CCCATGTACTCTGCGTTGATACCAC117990.26752978788531523No Hit
GTATCAACGCAGAGTACTTTTTTTT68220.1546816012334622No Hit
GAGTACATGGGAAGCAGTGGTATCA56400.1278810071763012No Hit
ATTCCATTCCATTCCATTCCATTCC48320.10956046572267507No Hit
CATGTACTCTGCGTTGATACCACTG44420.1007176301200585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCG700.001496468410.8535515
CGAACCG803.757501E-410.69245712
CAGGACG951.6506914E-49.9968054
CAGATCG951.6508803E-49.9966929
CCGTACA2105.456968E-129.95756217
GACAGCG1252.5842764E-69.8766197
GGACCGT800.00453057339.4968586
ATACCGT1201.7087663E-59.4968586
TATACCG1002.7665932E-49.4968575
GTCTTAC4450.09.3935611
TAGACTG6600.09.3529655
CCTACAC5650.09.0770543
CTACGCA950.00183015428.9971254
CTAATAC6150.08.9567923
TACGCCT850.0074588028.938324
TCTATAC7550.08.9312253
CCCGTAC2356.548362E-118.89784216
CTAGTAC2252.6375346E-108.8641363
CTGTGCG1401.0907253E-58.818519
GTCCTAA4100.08.8051611
Produced by FastQC (version 0.11.5)