##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4410350 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.03201197183897 31.0 30.0 34.0 25.0 34.0 2 30.12548346503112 31.0 30.0 34.0 25.0 34.0 3 30.12595009466369 31.0 30.0 34.0 25.0 34.0 4 33.77396215719841 35.0 35.0 37.0 28.0 37.0 5 34.09463897423107 35.0 35.0 37.0 28.0 37.0 6 34.10686249390638 37.0 35.0 37.0 29.0 37.0 7 34.04400194995862 37.0 35.0 37.0 29.0 37.0 8 33.89559400047615 37.0 35.0 37.0 28.0 37.0 9 35.085742401396715 39.0 35.0 39.0 27.0 39.0 10 35.163517634654845 38.0 35.0 39.0 27.0 39.0 11 35.401437300894486 39.0 35.0 39.0 28.0 39.0 12 35.16568435611686 38.0 35.0 39.0 27.0 39.0 13 35.03334655979684 38.0 35.0 39.0 27.0 39.0 14 35.942990465609306 39.0 35.0 41.0 27.0 41.0 15 35.91323182967338 39.0 35.0 41.0 27.0 41.0 16 36.02353690750167 39.0 35.0 41.0 27.0 41.0 17 35.98867323455055 39.0 35.0 41.0 27.0 41.0 18 35.872971306132165 39.0 35.0 41.0 27.0 41.0 19 36.14410307571961 39.0 35.0 41.0 27.0 41.0 20 36.258702824038906 39.0 35.0 41.0 27.0 41.0 21 36.28524448173047 39.0 36.0 41.0 27.0 41.0 22 36.274306120829415 39.0 35.0 41.0 27.0 41.0 23 36.23833595973109 39.0 35.0 41.0 27.0 41.0 24 36.28748806784042 39.0 35.0 41.0 27.0 41.0 25 34.97146825081909 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9893850272754747 1101 2 1.0189499758195737 1101 3 1.2446335875944747 1101 4 1.0541374865527047 1101 5 0.8249422258150716 1101 6 0.8450802942176381 1101 7 0.8031219033157342 1101 8 0.9694889397737114 1101 9 1.2100161676534853 1101 10 1.178622199981831 1101 11 0.9826024760228762 1101 12 1.1376970662690056 1101 13 1.2267890429738486 1101 14 1.404272630645245 1101 15 1.5262819263558853 1101 16 1.3899311329752138 1101 17 1.346361804468799 1101 18 1.4309936636066851 1101 19 1.224618937002731 1101 20 1.210308752948663 1101 21 1.0261524718328445 1101 22 0.9067516240389963 1101 23 1.0170522565384061 1101 24 1.038295816226558 1101 25 0.9160684729543789 1102 1 0.833414137752996 1102 2 0.7643590141245014 1102 3 1.0656456039616593 1102 4 0.8625539172698495 1102 5 0.7418381417013862 1102 6 0.6385533552545795 1102 7 0.6160754856925337 1102 8 0.6968718238100706 1102 9 0.9974586105297334 1102 10 0.8913463627679761 1102 11 0.6954087891304823 1102 12 0.9858901586381847 1102 13 0.8989755154416272 1102 14 1.087297762830957 1102 15 1.2089049972872346 1102 16 1.0992468591667262 1102 17 1.1695533670781515 1102 18 1.2266972962858773 1102 19 0.9021925120074741 1102 20 0.15221056524659815 1102 21 0.4675348673196069 1102 22 0.5623930818959479 1102 23 0.7756171290781353 1102 24 0.83214188954571 1102 25 0.7762997084995078 1103 1 0.6645180595502893 1103 2 0.6859810834253004 1103 3 0.9767377017868846 1103 4 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0.3696250203128031 2207 7 0.3670389930318194 2207 8 0.36134073354443075 2207 9 -1.2503126081024476 2207 10 -0.16614371635167657 2207 11 0.240543499944728 2207 12 0.5490561305787409 2207 13 0.6189336544775159 2207 14 0.8982252991223092 2207 15 0.8913941059504751 2207 16 0.8648777139604249 2207 17 0.8533330118062281 2207 18 0.8751350304147749 2207 19 -0.26854228781924405 2207 20 0.3473640194459051 2207 21 -0.10650665260700976 2207 22 0.3844822547118909 2207 23 -0.5507433592949553 2207 24 -1.6128037461901314 2207 25 -0.2632590715323957 2208 1 0.4432637238427297 2208 2 0.21498681463867797 2208 3 0.6611200698070228 2208 4 0.5127700438856451 2208 5 0.42236841917424073 2208 6 0.3653949438744206 2208 7 0.40042116361225055 2208 8 0.5379661431369271 2208 9 -0.19971991133962774 2208 10 0.024356660047743617 2208 11 0.22574859476323894 2208 12 0.20537263321458 2208 13 0.5387556203097859 2208 14 0.7879843274526834 2208 15 0.7548638823964993 2208 16 0.545419571081915 2208 17 0.46229438199852524 2208 18 0.6620086802337468 2208 19 -1.3100219289269859 2208 20 -0.1095499883826676 2208 21 -0.24114682773226548 2208 22 0.2366579238776012 2208 23 0.39014694722344245 2208 24 0.4611241491295317 2208 25 0.5554214475871859 2209 1 0.16778482852952337 2209 2 -1.3599190956107599 2209 3 -0.1696887160954823 2209 4 0.1670863595576506 2209 5 0.1071860782809253 2209 6 0.161977453205715 2209 7 0.18759705305972574 2209 8 0.34644848735953815 2209 9 -1.9301661363436722 2209 10 -0.22155122188783594 2209 11 0.07497791086311878 2209 12 0.3620365131328498 2209 13 0.48922258562709686 2209 14 0.7232721825319004 2209 15 0.7925616706307537 2209 16 0.7518743955475955 2209 17 0.5731171632967289 2209 18 0.769417546779323 2209 19 0.5300221288252942 2209 20 0.4566858667113536 2209 21 0.516625314895002 2209 22 0.4245778134927676 2209 23 0.4295439737875668 2209 24 0.44260066954122834 2209 25 0.4329354460655708 2210 1 0.08031841220924463 2210 2 0.10822862934584876 2210 3 0.07438178029446618 2210 4 0.15592700461135678 2210 5 0.09347782240880065 2210 6 0.09542883639009858 2210 7 0.02886152878374304 2210 8 0.16200210080906885 2210 9 0.27305629106925977 2210 10 -1.6038163831821421 2210 11 -0.4908368986903966 2210 12 -2.381463662891953 2210 13 -0.8680670029689637 2210 14 -0.6423611053026264 2210 15 -0.8359281600766195 2210 16 -0.16077629094913704 2210 17 -0.021940739599337178 2210 18 0.20863896127759318 2210 19 0.0745630129021535 2210 20 0.17834661441530386 2210 21 0.13939928051766515 2210 22 0.08089531282881524 2210 23 0.03625107468992894 2210 24 0.040999523218296474 2210 25 -0.02902293796496025 2211 1 -0.4874630958014272 2211 2 -0.15534120523379258 2211 3 -1.6186471154967599 2211 4 -0.7776117822243407 2211 5 -0.6074967587184901 2211 6 -0.4889125489202186 2211 7 -0.5320663122673324 2211 8 -0.1491977653415617 2211 9 0.005205765383784922 2211 10 -0.08790287019015608 2211 11 -0.37871642251948856 2211 12 -0.3954887359105612 2211 13 -0.44239783909104347 2211 14 -0.9131410279997638 2211 15 -0.4196104316974143 2211 16 -0.14204769146937934 2211 17 -0.2588728656678825 2211 18 -0.0834860811884397 2211 19 -0.16847633508501758 2211 20 -0.12153810513785146 2211 21 -0.34423522239810467 2211 22 -0.3609058519465407 2211 23 -0.6001771636466842 2211 24 -0.45342431319960497 2211 25 -0.2997301930141134 2212 1 -0.5200541269055279 2212 2 -0.45920616975212525 2212 3 -2.5700929222433864 2212 4 -1.550849934052927 2212 5 -0.8243286502878391 2212 6 -0.6077307695010816 2212 7 -0.43650751941606103 2212 8 -0.5240006415655998 2212 9 -1.1378641092503017 2212 10 -0.4165317852248691 2212 11 -0.4542983493898092 2212 12 -0.24668625527530708 2212 13 -0.6185243364344899 2212 14 -0.2772998398761146 2212 15 -0.14651074683673926 2212 16 -1.3471611980268037 2212 17 -1.5790474661282445 2212 18 -0.47654859606284816 2212 19 -0.40804879473085975 2212 20 -0.3425201658700203 2212 21 -0.2737787792260278 2212 22 -0.5282139819224341 2212 23 -0.3082645169270535 2212 24 -0.4555502148450543 2212 25 -0.2583547542846105 2213 1 -2.8382398736630776 2213 2 -1.331352685711213 2213 3 -2.310785090124103 2213 4 -0.9342457129471029 2213 5 -0.7805624424350484 2213 6 -0.6054745905169412 2213 7 -0.3493477494567756 2213 8 -0.17469726171201216 2213 9 -0.22115427072861138 2213 10 -0.15164001560699347 2213 11 -0.4403244312504455 2213 12 -0.2399352475969181 2213 13 -0.5111629985142017 2213 14 -0.17175186146017296 2213 15 -0.21106531439068732 2213 16 -1.5088262445807672 2213 17 -1.0679616587177065 2213 18 -0.23382937578308116 2213 19 -0.06376181735030428 2213 20 -0.05641483130131064 2213 21 0.06031550583983858 2213 22 -0.0019217133630817784 2213 23 0.05225487971024023 2213 24 0.12070747941596949 2213 25 0.0029057370142169248 2214 1 -0.8512470724660126 2214 2 -0.7538278911385277 2214 3 -6.492666747742625 2214 4 -2.0416825444875712 2214 5 -1.2561664466039986 2214 6 -0.7816972668496547 2214 7 -0.585508201444469 2214 8 -0.3723475976464883 2214 9 -0.2598583537555825 2214 10 -0.15707206647203975 2214 11 -0.8472314588976957 2214 12 -0.546333502917058 2214 13 -5.251521131140549 2214 14 -3.971102975266959 2214 15 -2.4251163694781894 2214 16 -2.775691364009255 2214 17 -3.5015944440260967 2214 18 -1.1215365111365543 2214 19 -0.7535196102195982 2214 20 -0.3756491711362955 2214 21 -0.43751251342121833 2214 22 -0.394578716782938 2214 23 -0.3730047761702977 2214 24 -0.2647621030681222 2214 25 -0.035184871135072626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1628.0 3 214.0 4 213.0 5 509.0 6 76.0 7 121.0 8 267.0 9 845.0 10 1995.0 11 3504.0 12 6489.0 13 10410.0 14 15504.0 15 20160.0 16 24277.0 17 27209.0 18 29071.0 19 30192.0 20 31268.0 21 32611.0 22 33629.0 23 35540.0 24 38808.0 25 41796.0 26 46559.0 27 53094.0 28 61854.0 29 75070.0 30 91968.0 31 113903.0 32 147701.0 33 193637.0 34 258353.0 35 350095.0 36 488388.0 37 729183.0 38 1414209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16684831430837 21.2599234764862 13.17470448024338 23.398523728962047 2 14.612362011574684 21.302071269755533 41.377551031982485 22.7080156866873 3 18.262763793536056 27.095681433644792 29.707447858366958 24.934106914452194 4 11.778573329283235 17.48494767500816 39.21882397564678 31.517655020061824 5 12.567994252629768 38.63584343724317 35.540121457884794 13.25604085224227 6 30.178716923800884 39.18732774556021 17.892816131921123 12.741139198717782 7 25.590321350353495 33.077757133940636 24.015094983456823 17.316826532249046 8 23.77968737309089 37.99539077456132 21.521334796838794 16.703587055508994 9 24.3215679298786 17.2223933973493 22.37565463141659 36.08038404135551 10 14.34979409862846 30.453300661380162 34.10666031378688 21.090244926204495 11 32.18781861551788 26.390632664071532 23.20210665687783 18.219442063532757 12 20.48614049468464 27.8059948313144 32.457902618986736 19.249962055014215 13 26.312547516612522 24.18282770325993 27.219625611632814 22.28499916849473 14 19.877056944542446 23.23305658969831 28.90060257706686 27.989283888692384 15 22.62316320152287 30.74270584111275 24.4893606642367 22.14477029312768 16 21.237800254737532 31.742615266011054 26.21634449371709 20.80323998553432 17 19.68256561222001 30.511046448970795 29.207956846529516 20.598431092279682 18 20.04861751602003 30.280343147205357 29.80336380896849 19.86767552780612 19 20.49843645840471 29.181685303162798 30.04341431560624 20.276463922826256 20 21.8616387817439 28.838938564685968 29.026501850562408 20.27292080300773 21 21.982795096849898 29.348614480532575 28.357121644250956 20.31146877836657 22 20.630896746818824 29.05872815524949 29.640090130051465 20.670284967880225 23 20.557243847842237 29.46247264308523 29.210650626049638 20.769632883022897 24 20.692494534579915 28.705713647156788 29.32938983562331 21.272401982639987 25 21.272415063721496 28.68874032742012 28.43026031333441 21.608584295523976 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3692.0 1 3692.0 2 3462.5 3 3233.0 4 3233.0 5 3233.0 6 5138.5 7 7044.0 8 7044.0 9 7044.0 10 11524.5 11 16005.0 12 16005.0 13 16005.0 14 27583.5 15 39162.0 16 39162.0 17 39162.0 18 64508.5 19 89855.0 20 89855.0 21 89855.0 22 130730.5 23 171606.0 24 171606.0 25 171606.0 26 225823.0 27 280040.0 28 280040.0 29 280040.0 30 337659.5 31 395279.0 32 395279.0 33 395279.0 34 442795.0 35 490311.0 36 490311.0 37 490311.0 38 518375.0 39 546439.0 40 546439.0 41 546439.0 42 539535.5 43 532632.0 44 532632.0 45 532632.0 46 531353.0 47 530074.0 48 530074.0 49 530074.0 50 488174.5 51 446275.0 52 446275.0 53 446275.0 54 392669.5 55 339064.0 56 339064.0 57 339064.0 58 288434.5 59 237805.0 60 237805.0 61 237805.0 62 194304.0 63 150803.0 64 150803.0 65 150803.0 66 114028.5 67 77254.0 68 77254.0 69 77254.0 70 55471.0 71 33688.0 72 33688.0 73 33688.0 74 23472.0 75 13256.0 76 13256.0 77 13256.0 78 8910.5 79 4565.0 80 4565.0 81 4565.0 82 3050.5 83 1536.0 84 1536.0 85 1536.0 86 1015.0 87 494.0 88 494.0 89 494.0 90 327.0 91 160.0 92 160.0 93 160.0 94 102.5 95 45.0 96 45.0 97 45.0 98 39.0 99 33.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09940254174838732 2 0.05171925130658565 3 0.05559649460927138 4 0.05049485868468489 5 0.04700307231852348 6 0.06777239901595111 7 0.06332830727720022 8 0.0604940650968744 9 0.06593581008309998 10 0.06575441858355913 11 0.059133628850318004 12 0.060153956035235295 13 0.06078882628362828 14 0.06305622002788894 15 0.06541430952192002 16 0.06067545659641525 17 0.06405387327536363 18 0.14003423764553832 19 0.0691101613250649 20 0.06282948065346287 21 0.060153956035235295 22 0.066684050018706 23 0.06511954833516614 24 0.07017583638486741 25 0.06906481345017969 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4410350.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.1972117907487 #Duplication Level Percentage of deduplicated Percentage of total 1 90.0385755037959 72.20842709015238 2 7.563487701212676 12.13141250101752 3 1.2655422755504284 3.0447888570739123 4 0.3990716327610851 1.280177290088825 5 0.18164821813852788 0.728384031073382 6 0.11702929017144773 0.5631253661760347 7 0.07374706423112408 0.41400162503625837 8 0.05396429479422346 0.3462228783000589 9 0.04207687912949391 0.3037003548337582 >10 0.2343598257440408 3.3940769438818417 >50 0.016794658367088454 0.9352718192587598 >100 0.011712340399289173 1.7347880688162354 >500 9.66725080464912E-4 0.5338303319753916 >1k 9.098583325971248E-4 1.5486567420633084 >5k 5.686614578725186E-5 0.2841651498848788 >10k+ 5.686614578725186E-5 0.5489709503673935 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 12276 0.2783452560454386 No Hit CCCATGTACTCTGCGTTGATACCAC 11799 0.26752978788531523 No Hit GTATCAACGCAGAGTACTTTTTTTT 6822 0.1546816012334622 No Hit GAGTACATGGGAAGCAGTGGTATCA 5640 0.1278810071763012 No Hit ATTCCATTCCATTCCATTCCATTCC 4832 0.10956046572267507 No Hit CATGTACTCTGCGTTGATACCACTG 4442 0.1007176301200585 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.2673937442606595E-5 0.0 0.0 0.0 3 0.0 2.2673937442606595E-5 0.0 0.0 0.0 4 0.0 2.2673937442606595E-5 0.0 2.2673937442606595E-5 0.0 5 0.0 2.2673937442606595E-5 0.0 2.2673937442606595E-5 0.0 6 0.0 2.2673937442606595E-5 0.0 2.2673937442606595E-5 0.0 7 0.0 4.534787488521319E-5 0.0 2.2673937442606595E-5 0.0 8 0.0 4.534787488521319E-5 0.0 2.2673937442606595E-5 0.0 9 0.0 6.802181232781979E-5 0.0 2.2673937442606595E-5 0.0 10 0.0 6.802181232781979E-5 0.0 4.534787488521319E-5 0.0 11 0.0 9.069574977042638E-5 0.0 6.802181232781979E-5 0.0 12 0.0 9.069574977042638E-5 0.0 6.802181232781979E-5 0.0 13 0.0 9.069574977042638E-5 0.0 6.802181232781979E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCCG 70 0.0014964684 10.853551 5 CGAACCG 80 3.757501E-4 10.692457 12 CAGGACG 95 1.6506914E-4 9.996805 4 CAGATCG 95 1.6508803E-4 9.996692 9 CCGTACA 210 5.456968E-12 9.957562 17 GACAGCG 125 2.5842764E-6 9.876619 7 GGACCGT 80 0.0045305733 9.496858 6 ATACCGT 120 1.7087663E-5 9.496858 6 TATACCG 100 2.7665932E-4 9.496857 5 GTCTTAC 445 0.0 9.393561 1 TAGACTG 660 0.0 9.352965 5 CCTACAC 565 0.0 9.077054 3 CTACGCA 95 0.0018301542 8.997125 4 CTAATAC 615 0.0 8.956792 3 TACGCCT 85 0.007458802 8.93832 4 TCTATAC 755 0.0 8.931225 3 CCCGTAC 235 6.548362E-11 8.897842 16 CTAGTAC 225 2.6375346E-10 8.864136 3 CTGTGCG 140 1.0907253E-5 8.81851 9 GTCCTAA 410 0.0 8.805161 1 >>END_MODULE