FastQCFastQC Report
Wed 25 May 2016
SRR1294643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410350
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT168020.38096749691067605No Hit
CCCATGTACTCTGCGTTGATACCAC133800.3033772829820763No Hit
GTACATGGGAAGCAGTGGTATCAAC119570.27111227000124705No Hit
GGTATCAACGCAGAGTACTTTTTTT113420.25716779847404403No Hit
TATCAACGCAGAGTACTTTTTTTTT99640.22592311267813212No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63230.1433673064496015No Hit
GAGTACATGGGAAGCAGTGGTATCA57630.13066990148174182No Hit
CATGTACTCTGCGTTGATACCACTG50100.11359642658745905No Hit
ATTCCATTCCATTCCATTCCATTCC45740.10371058986248258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG2250.011.4270615
TACACCG1158.5630745E-710.7646235
CGCCCTA2500.09.8610116
TAAACCG1002.6925263E-49.5225525
CCGCCCT2950.09.32071615
GCGTGCC1451.7384464E-69.16060111
TAGACTG6800.09.1024395
CAGCGTA1054.3913114E-49.0688910
AGACCGT950.00178589039.0213646
CAGGACT6350.08.9976864
TGTCCCG2850.08.98840811
TAGACAG8500.08.96245
CCCCCGG1804.3590262E-88.95497818
CGAGACA850.0075575848.92399217
GCACTGT7650.08.8379236
CCAGGAC6650.08.7121553
TGTACCG1106.982229E-48.6568645
GTATTAA7250.08.6547141
CCCCGTA2850.08.64971515
GTCCCGC2751.8189894E-128.61956912
Produced by FastQC (version 0.11.5)