##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294643_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4410350 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.352085662135657 31.0 31.0 34.0 28.0 34.0 2 31.491164873536114 31.0 31.0 34.0 28.0 34.0 3 31.596157674560978 31.0 31.0 34.0 28.0 34.0 4 35.196352216944234 37.0 35.0 37.0 32.0 37.0 5 35.304205788656226 37.0 35.0 37.0 32.0 37.0 6 34.91297402700466 37.0 35.0 37.0 32.0 37.0 7 34.76665434716065 37.0 35.0 37.0 32.0 37.0 8 34.75102973686896 37.0 35.0 37.0 32.0 37.0 9 36.24229755008106 39.0 35.0 39.0 31.0 39.0 10 35.99179543573639 39.0 35.0 39.0 30.0 39.0 11 36.17769451404084 39.0 35.0 39.0 31.0 39.0 12 36.116658315099706 39.0 35.0 39.0 30.0 39.0 13 35.985421111703154 39.0 35.0 39.0 30.0 39.0 14 37.052222159239065 40.0 36.0 41.0 30.0 41.0 15 37.11414853696419 40.0 36.0 41.0 31.0 41.0 16 37.15242191662793 40.0 36.0 41.0 31.0 41.0 17 37.17879737435804 40.0 36.0 41.0 31.0 41.0 18 37.177387508927865 40.0 36.0 41.0 31.0 41.0 19 37.192662033625446 40.0 37.0 41.0 30.0 41.0 20 37.262072851361005 40.0 37.0 41.0 31.0 41.0 21 37.14161120999467 40.0 37.0 41.0 30.0 41.0 22 37.1002677792012 40.0 36.0 41.0 30.0 41.0 23 37.109742764179714 40.0 36.0 41.0 30.0 41.0 24 37.03828653054746 40.0 36.0 41.0 30.0 41.0 25 35.93501581507137 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4967209310878715 1101 2 0.4749033876558322 1101 3 0.42049990789914915 1101 4 0.3350014965265302 1101 5 0.3951699829958315 1101 6 0.47828195437437415 1101 7 0.5658951656027824 1101 8 0.5141650737121495 1101 9 0.5995960817357897 1101 10 0.7079010126010985 1101 11 0.6915256350131571 1101 12 0.727250426507382 1101 13 0.7318159459014666 1101 14 0.8722734876640814 1101 15 0.886989958374464 1101 16 0.9487405603509131 1101 17 0.8871390200762548 1101 18 0.8910992561561031 1101 19 1.075244135307031 1101 20 1.0061776216397789 1101 21 1.2147685634290326 1101 22 1.0843858922482212 1101 23 1.0578427590759958 1101 24 1.2269657545059545 1101 25 1.0874810145320737 1102 1 0.37992646755566994 1102 2 0.3531693103366571 1102 3 0.2972625713253869 1102 4 0.24316113089374625 1102 5 0.2996252363113143 1102 6 0.32125533729798406 1102 7 0.3678901413475302 1102 8 0.3672726537243136 1102 9 0.4409606040156362 1102 10 0.641060659137672 1102 11 0.6135846227236286 1102 12 0.6345442468818874 1102 13 0.6509262123923563 1102 14 0.8328977991826818 1102 15 0.8030784302333132 1102 16 0.7729388008450755 1102 17 0.6750118430175718 1102 18 0.6475362950645192 1102 19 0.8541882147614217 1102 20 0.7398758009005988 1102 21 1.0705536597915923 1102 22 0.9545989931599976 1102 23 0.951616308751305 1102 24 1.0400733723147084 1102 25 0.9433337152546741 1103 1 0.2828545688614703 1103 2 0.27528681379262565 1103 3 0.22500369936200215 1103 4 0.14514360394780823 1103 5 0.18751428570707418 1103 6 0.20335850784889686 1103 7 0.27843934441558105 1103 8 0.35507976225719773 1103 9 0.3441137391263993 1103 10 0.5309255283896874 1103 11 0.4926719580172758 1103 12 0.4543676254207014 1103 13 0.5434027358376312 1103 14 0.6844226393197204 1103 15 0.6574132415772098 1103 16 0.6563182498057003 1103 17 0.6342864583807994 1103 18 0.6605971379260183 1103 19 0.771325980957144 1103 20 0.6405726495868933 1103 21 0.8988468795702715 1103 22 0.825668128879812 1103 23 0.8628264186212604 1103 24 0.8982562026963663 1103 25 0.955140681897646 1104 1 0.15532859352121164 1104 2 0.16195349548693017 1104 3 0.10578976054958744 1104 4 0.09091040624446123 1104 5 0.12345554579058415 1104 6 0.11222846766639094 1104 7 0.18210526134230065 1104 8 0.1187034910408542 1104 9 -0.10782822536368286 1104 10 0.27374933772147614 1104 11 0.33304416698503303 1104 12 0.41820574571042357 1104 13 -0.10748617811587735 1104 14 0.22869647926754055 1104 15 0.3903027670442256 1104 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0.23628844910292912 2207 5 0.23342393818859364 2207 6 0.3057468203512812 2207 7 0.26665144475114744 2207 8 0.30920999526588133 2207 9 0.44721142940641556 2207 10 0.5607761780369955 2207 11 0.5002369632412353 2207 12 0.5086789668274747 2207 13 0.4881847955211711 2207 14 0.5829818417134973 2207 15 0.5824486146827823 2207 16 0.5671041715250311 2207 17 0.6252334429677191 2207 18 0.654807175736515 2207 19 0.6812220691483901 2207 20 0.6027073284207063 2207 21 0.8938722248902309 2207 22 0.8182931709485004 2207 23 0.6725993704352788 2207 24 0.8856423206380413 2207 25 0.8273119002795397 2208 1 0.17021866077966763 2208 2 0.18932554133844448 2208 3 0.1693926663360834 2208 4 0.1694711779383411 2208 5 0.10300053245148888 2208 6 0.25253975415105145 2208 7 0.305874776714262 2208 8 0.19549144780235395 2208 9 0.2738564976495894 2208 10 0.38669811806029486 2208 11 0.024914421962911604 2208 12 -0.16726047737170546 2208 13 -0.003533261706152757 2208 14 -0.45762445433280874 2208 15 0.1501762446367536 2208 16 0.2509197866514441 2208 17 0.29688443862582403 2208 18 0.2792928787461122 2208 19 0.5589635637813615 2208 20 0.4299694412284154 2208 21 0.668066827789211 2208 22 0.7254675869404608 2208 23 0.6432243738831858 2208 24 0.7210794725207563 2208 25 0.705053506736462 2209 1 0.05468521425009598 2209 2 0.08060065135951788 2209 3 0.15216918021248915 2209 4 -0.46302509509394696 2209 5 -0.013713373299694354 2209 6 0.1396057519981042 2209 7 0.2189234301352414 2209 8 0.22035669125441615 2209 9 0.2833077424999644 2209 10 0.34458657660617575 2209 11 0.3703939584748639 2209 12 0.36121625636188526 2209 13 0.40939558619533756 2209 14 0.44169233607288305 2209 15 -0.19631400605962313 2209 16 -0.13990527583460732 2209 17 0.2534570710302759 2209 18 -0.07832923570539663 2209 19 0.3693389363239987 2209 20 0.40257738148842037 2209 21 0.655200916010763 2209 22 0.6650177120358691 2209 23 0.6446168972591977 2209 24 0.7311871692485497 2209 25 0.7420073934489793 2210 1 -0.13497490347035423 2210 2 -0.08921739457986888 2210 3 -0.07174777313785086 2210 4 -0.035118740289703965 2210 5 -0.041105673913001795 2210 6 -0.5252351771658113 2210 7 -0.14790046476868923 2210 8 -0.2532896780023819 2210 9 0.020732654024335773 2210 10 0.2582636421182869 2210 11 0.17270414506115372 2210 12 0.11131038330973553 2210 13 0.12775528207389186 2210 14 0.16976075722303108 2210 15 0.09784808499151865 2210 16 0.1615473920220225 2210 17 -0.9349176950420244 2210 18 -1.4966595323535117 2210 19 -0.32797819860982 2210 20 -0.10941571339821365 2210 21 -0.31679112890335404 2210 22 -0.29957258251230456 2210 23 -0.18298156854567083 2210 24 0.255768261096442 2210 25 0.22936665080959528 2211 1 -0.2876079507359748 2211 2 -0.261927706417751 2211 3 -0.22604218354849337 2211 4 -0.13165385647754135 2211 5 -0.10151091792902633 2211 6 -0.12804278503719502 2211 7 -0.2865433817659948 2211 8 -0.20130123386854137 2211 9 -0.19976642493173102 2211 10 -0.02156395152586299 2211 11 -0.4691780976150923 2211 12 -0.31566111907793015 2211 13 -0.19830195307908127 2211 14 -0.34048031457244576 2211 15 -0.3836962084540687 2211 16 -0.30970608279073275 2211 17 -0.47198341523405674 2211 18 -0.47416538571086164 2211 19 -0.3810324732006194 2211 20 -0.5151689772527348 2211 21 -0.12369205408222683 2211 22 -0.22975971127748807 2211 23 -0.8634291216215146 2211 24 -0.23462580072899897 2211 25 -0.8993964524027191 2212 1 -0.24702718903611398 2212 2 -0.37271711682787867 2212 3 -0.23995865167866626 2212 4 -0.08760696795926037 2212 5 -0.13024486306603933 2212 6 -0.13699767220220593 2212 7 -0.2851520944306074 2212 8 -0.4742015175808092 2212 9 -0.21200119137979812 2212 10 -0.6680892775822755 2212 11 -0.2994967889618607 2212 12 -0.3666872792722202 2212 13 -0.34754503890145116 2212 14 -0.4473445425156868 2212 15 -0.33509699879830634 2212 16 -0.4497906591609251 2212 17 -0.7075051475763274 2212 18 -0.5253766224149246 2212 19 -0.5279402208121482 2212 20 -0.712861490387759 2212 21 -0.21358478514238755 2212 22 -0.5837194485309354 2212 23 -0.407593017382915 2212 24 -0.7432151508939455 2212 25 -0.48064813115640703 2213 1 -0.24544253133639415 2213 2 -0.2513755173798913 2213 3 -0.1331208981647265 2213 4 -0.09451556919172077 2213 5 -0.13022234970117097 2213 6 -0.2957857372756294 2213 7 -0.3786620208988367 2213 8 -0.377033126843493 2213 9 -0.3026993543288725 2213 10 -0.36691287355964164 2213 11 -0.11471051668856802 2213 12 -0.0626425592906159 2213 13 0.07509566948738211 2213 14 -0.7505083810789444 2213 15 -0.2298213613005231 2213 16 -0.3935828295959922 2213 17 -0.2770268430688816 2213 18 -0.25141015435860936 2213 19 -0.35620000453998557 2213 20 -0.4169997696836347 2213 21 -0.13784651243910417 2213 22 -0.36553753046134574 2213 23 -0.5446162090938742 2213 24 -0.7223388049982731 2213 25 -0.45942077499967837 2214 1 -0.2290118642265888 2214 2 -0.12407692300631012 2214 3 -0.1501413596849126 2214 4 -0.06290252966805099 2214 5 -0.13492879084149223 2214 6 -0.12757746528929204 2214 7 -0.18232765080843194 2214 8 -0.12816406871176156 2214 9 -0.168892415591273 2214 10 -0.12408600048050289 2214 11 0.01967684478882603 2214 12 -0.2784824209139103 2214 13 -0.1680888643825753 2214 14 -0.1000693286594938 2214 15 0.011808746489059274 2214 16 -0.23492842883982235 2214 17 -0.40626927565183735 2214 18 -0.284913728867501 2214 19 -0.3860489822858426 2214 20 -0.4135284969877162 2214 21 -0.5054698916648519 2214 22 -0.86962428836177 2214 23 -1.1883713091895203 2214 24 -0.5751702586661835 2214 25 -0.789530982889346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 20.0 9 50.0 10 96.0 11 271.0 12 705.0 13 1591.0 14 3518.0 15 6155.0 16 9043.0 17 11840.0 18 13814.0 19 15683.0 20 17547.0 21 19147.0 22 21301.0 23 24058.0 24 27185.0 25 30957.0 26 36188.0 27 40757.0 28 48189.0 29 60312.0 30 76768.0 31 99800.0 32 134098.0 33 181120.0 34 246704.0 35 343685.0 36 490361.0 37 770768.0 38 1678616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.304875261599875 21.366953385348047 12.238913623756003 25.089257729296076 2 14.425816545172152 21.684129377487046 40.184611198657706 23.705442878683098 3 17.859317510935053 26.962985868090982 29.823513466020625 25.35418315495334 4 11.727194740292534 17.78296518208084 39.104731319391895 31.385108758234733 5 12.906288937038966 38.2632906982915 35.47764960100804 13.3527707636615 6 30.516618981284218 39.42585242392694 17.387540996692145 12.669987598096697 7 25.740428786031526 33.50614313913321 23.499843065110742 17.25358500972452 8 23.962849589094905 38.1325328254641 21.084994468803277 16.81962311663772 9 24.258758457687023 16.745636444561946 22.404750475984034 36.590854621767 10 14.217010968627989 30.497853192074942 33.96748452752583 21.31765131177124 11 32.35655534994568 26.092800336881538 23.046254960880177 18.5043893522926 12 20.604868899420854 27.666804677618963 32.41861397399438 19.3097124489658 13 26.59082684631689 23.752479396183706 27.261726285544814 22.394967471954594 14 19.932453574586763 22.943133120195906 29.06663945763327 28.057773847584066 15 22.753464567304064 30.430826446599557 24.602957038435786 22.212751947660596 16 21.20918802779282 31.49717437263454 26.334316475706682 20.959321123865962 17 19.501345358993294 30.467217205358217 29.319597755006576 20.71183968064191 18 19.87160170680757 30.071497905263065 30.096280580699126 19.960619807230238 19 20.36213633090328 28.957710174844138 30.490634710162144 20.189518784090435 20 21.669407950675932 28.468948666565996 29.62253267433682 20.23911070842125 21 21.80648613595808 29.132717959880555 28.750751077583764 20.310044826577602 22 20.387523320874372 28.860177243180146 30.07696682154962 20.67533261439586 23 20.306316033636357 29.223913673912733 29.77330355185204 20.69646674059887 24 20.473862618613033 28.507918872651832 29.86440985409321 21.153808654641924 25 21.096381108380367 28.423727964232597 28.959431763067727 21.520459164319306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1731.0 1 1731.0 2 1991.0 3 2251.0 4 2251.0 5 2251.0 6 4622.5 7 6994.0 8 6994.0 9 6994.0 10 11031.0 11 15068.0 12 15068.0 13 15068.0 14 25185.5 15 35303.0 16 35303.0 17 35303.0 18 59776.0 19 84249.0 20 84249.0 21 84249.0 22 125853.0 23 167457.0 24 167457.0 25 167457.0 26 223492.5 27 279528.0 28 279528.0 29 279528.0 30 338120.5 31 396713.0 32 396713.0 33 396713.0 34 444234.5 35 491756.0 36 491756.0 37 491756.0 38 518800.0 39 545844.0 40 545844.0 41 545844.0 42 539382.5 43 532921.0 44 532921.0 45 532921.0 46 533827.5 47 534734.0 48 534734.0 49 534734.0 50 493305.5 51 451877.0 52 451877.0 53 451877.0 54 397779.0 55 343681.0 56 343681.0 57 343681.0 58 292334.0 59 240987.0 60 240987.0 61 240987.0 62 196636.5 63 152286.0 64 152286.0 65 152286.0 66 114442.5 67 76599.0 68 76599.0 69 76599.0 70 54568.5 71 32538.0 72 32538.0 73 32538.0 74 22411.0 75 12284.0 76 12284.0 77 12284.0 78 8119.5 79 3955.0 80 3955.0 81 3955.0 82 2570.5 83 1186.0 84 1186.0 85 1186.0 86 740.5 87 295.0 88 295.0 89 295.0 90 189.5 91 84.0 92 84.0 93 84.0 94 52.0 95 20.0 96 20.0 97 20.0 98 14.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0972485176913397 2 0.0 3 0.12942283492239845 4 0.01185846928248325 5 0.022401850193295315 6 0.012311948031335382 7 0.019907717074608592 8 0.01850193295316698 9 2.9476118675388573E-4 10 0.39790492818030315 11 0.06498350471051051 12 0.001632523495867675 13 4.534787488521319E-5 14 0.0011336968721303298 15 0.0013377623091137892 16 4.534787488521319E-5 17 6.802181232781979E-5 18 2.494133118686726E-4 19 4.988266237373452E-4 20 1.8139149954085276E-4 21 4.534787488521319E-4 22 0.00312900336707971 23 6.802181232781979E-5 24 0.0 25 0.001745893183080708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4410350.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.22878678029555 #Duplication Level Percentage of deduplicated Percentage of total 1 89.16833197107326 69.75530429319701 2 8.23322482243219 12.881503782965689 3 1.3736182088433713 3.2236945793141873 4 0.4403881510972349 1.3780412309101666 5 0.20738560905813153 0.811176229615515 6 0.11640816774759057 0.5463881840526684 7 0.08144907211091797 0.44601634669325424 8 0.055956812421653686 0.3501946838270832 9 0.043354675081629074 0.3052425269600778 >10 0.2459590307637911 3.509757108333642 >50 0.019348059919471663 1.0452606104381954 >100 0.012276564813901167 1.7875556923621714 >500 0.0011057785074444627 0.5891584968481818 >1k 9.602807871455195E-4 1.5372611248294632 >5k 1.1639767116907795E-4 0.6159971277635321 >10k+ 1.1639767116907795E-4 1.2174479818891892 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16802 0.38096749691067605 No Hit CCCATGTACTCTGCGTTGATACCAC 13380 0.3033772829820763 No Hit GTACATGGGAAGCAGTGGTATCAAC 11957 0.27111227000124705 No Hit GGTATCAACGCAGAGTACTTTTTTT 11342 0.25716779847404403 No Hit TATCAACGCAGAGTACTTTTTTTTT 9964 0.22592311267813212 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6323 0.1433673064496015 No Hit GAGTACATGGGAAGCAGTGGTATCA 5763 0.13066990148174182 No Hit CATGTACTCTGCGTTGATACCACTG 5010 0.11359642658745905 No Hit ATTCCATTCCATTCCATTCCATTCC 4574 0.10371058986248258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.2673937442606595E-5 0.0 5 0.0 0.0 0.0 2.2673937442606595E-5 0.0 6 0.0 0.0 0.0 2.2673937442606595E-5 0.0 7 0.0 0.0 0.0 4.534787488521319E-5 0.0 8 0.0 0.0 0.0 4.534787488521319E-5 0.0 9 0.0 0.0 0.0 6.802181232781979E-5 0.0 10 0.0 0.0 0.0 6.802181232781979E-5 0.0 11 0.0 0.0 0.0 6.802181232781979E-5 0.0 12 0.0 0.0 0.0 9.069574977042638E-5 0.0 13 0.0 0.0 0.0 9.069574977042638E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 225 0.0 11.427061 5 TACACCG 115 8.5630745E-7 10.764623 5 CGCCCTA 250 0.0 9.86101 16 TAAACCG 100 2.6925263E-4 9.522552 5 CCGCCCT 295 0.0 9.320716 15 GCGTGCC 145 1.7384464E-6 9.160601 11 TAGACTG 680 0.0 9.102439 5 CAGCGTA 105 4.3913114E-4 9.06889 10 AGACCGT 95 0.0017858903 9.021364 6 CAGGACT 635 0.0 8.997686 4 TGTCCCG 285 0.0 8.988408 11 TAGACAG 850 0.0 8.9624 5 CCCCCGG 180 4.3590262E-8 8.954978 18 CGAGACA 85 0.007557584 8.923992 17 GCACTGT 765 0.0 8.837923 6 CCAGGAC 665 0.0 8.712155 3 TGTACCG 110 6.982229E-4 8.656864 5 GTATTAA 725 0.0 8.654714 1 CCCCGTA 285 0.0 8.649715 15 GTCCCGC 275 1.8189894E-12 8.619569 12 >>END_MODULE