FastQCFastQC Report
Wed 25 May 2016
SRR1294642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741311
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC533061.4247946775876157No Hit
CCCATGTACTCTGCGTTGATACCAC524061.4007389388372151No Hit
GAGTACATGGGAAGCAGTGGTATCA237480.634750759827237No Hit
CATGTACTCTGCGTTGATACCACTG194080.518748641853083No Hit
GCGTTGATACCACTGCTTCCCATGT164870.4406744053087273No Hit
GTATCAACGCAGAGTACTTTTTTTT156040.41707305273472317No Hit
GTATCAACGCAGAGTACATGGGAAG147290.3936855289496115No Hit
GTACTGGTTCACTATCGGTCAGTCA134710.3600609518962738No Hit
ACGCAGAGTACATGGGAAGCAGTGG122440.3272649613998943No Hit
TATCAACGCAGAGTACATGGGAAGC116030.3101319296898868No Hit
ACTCTGCGTTGATACCACTGCTTCC107980.28861540780758405No Hit
GTTAATGATAGTGTGTCGAAACACA105290.2814254147810754No Hit
GGTATCAACGCAGAGTACATGGGAA97040.2593743209265415No Hit
GGATACCACGTGTCCCGCCCTACTC86890.23224479333581197No Hit
GGTATCAACGCAGAGTACTTTTTTT86470.2311221921941266No Hit
TATCAACGCAGAGTACTTTTTTTTT85720.22911754729825987No Hit
GCTTCCCATGTACTCTGCGTTGATA79570.2126794591521528No Hit
GTACATGGGGGTTAAGCGACTAAGC71720.19169750924208118No Hit
ATACAGGGTGACAGCCCCGTACACA71570.19129658026290783No Hit
GTGGTATCAACGCAGAGTACATGGG70670.1888910063878678No Hit
ATTCCATTCCATTCCATTCCATTCC69950.18696654728783574No Hit
ACTTAGATGTTTCAGTTCCCCCGGT66930.17889451050714575No Hit
AGCGTACACGGTGGATGCCCTGGCA65040.17384280536956162No Hit
CAGTGGTATCAACGCAGAGTACATG64080.17127685990285224No Hit
ATACCACTGCTTCCCATGTACTCTG61370.1640334096791205No Hit
GTGGATGCCCTGGCAGTCAGAGGCG59650.15943609071793283No Hit
TCATTAACCTATGGATTCAGTTAAT59580.15924899052765196No Hit
GTATCAACGCAGAGTACATGGGGGT58240.1556673583137034No Hit
GTACTCTGCGTTGATACCACTGCTT56580.15123041094418507No Hit
ACCCTGTATCGCGCGCCTTTCCAGA54590.1459114198204854No Hit
GAATGGAATGGAATGGAATGGAATG51920.13877488399119986No Hit
GCAGTGGTATCAACGCAGAGTACAT51240.13695733928561404No Hit
GGGAAGCAGTGGTATCAACGCAGAG51060.13647622451060604No Hit
CTGCTTCCCATGTACTCTGCGTTGA50510.13500615158697044No Hit
GTACTTAGATGTTTCAGTTCCCCCG50150.13404392203695442No Hit
GTTCACTATCGGTCAGTCAGGAGTA49740.13294804949388062No Hit
ATCATTAACTGAATCCATAGGTTAA48550.12976734625910544No Hit
GTATTTAGCCTTGGAGGATGGTCCC48410.12939314587854364No Hit
CTATCGGTCAGTCAGGAGTATTTAG47940.12813690174380052No Hit
CATGGGAAGCAGTGGTATCAACGCA47910.12805671594796583No Hit
TACCACTGCTTCCCATGTACTCTGC45640.12198932406314257No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45480.12156166648535768No Hit
GGTATCAACGCAGAGTACATGGGGG44870.11993122197005274No Hit
CCTATGGATTCAGTTAATGATAGTG44120.11792657707418602No Hit
CCCATATTCAGACAGGATACCACGT43990.1175791052922358No Hit
GCAGAGTACATGGGAAGCAGTGGTA43810.11709799051722777No Hit
TATTCAGACAGGATACCACGTGTCC43440.1161090323686002No Hit
CCATGTACTCTGCGTTGATACCACT41200.11012182627961162No Hit
ACATGGGAAGCAGTGGTATCAACGC40710.10881212494764536No Hit
GTCAGGAGTATTTAGCCTTGGAGGA40270.10763606660873688No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39790.10635309387538219No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39420.1053641357267546No Hit
GTTGATACCACTGCTTCCCATGTAC39290.10501666394480437No Hit
GATACCACTGCTTCCCATGTACTCT38940.10408116299339991No Hit
AAGCAGTGGTATCAACGCAGAGTAC38130.10191614650586385No Hit
GCCTTGGAGGATGGTCCCCCCATAT38120.10188941790725228No Hit
CCACTGCTTCCCATGTACTCTGCGT37830.10111428854751717No Hit
GCCCAGAGCCTGAATCAGTGTGTGT37820.10108755994890561No Hit
GTACATGGTAAGCAGTGGTATCAAC37760.10092718835723628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC5200.013.16133418
AAATCGC5600.012.55954117
ATCGCCG5600.012.55181319
GAAATCG5700.012.505609516
GGACCGT1001.4501893E-712.3462896
ACGACTA550.003059535312.09633516
ACGGAGC550.00307644312.0874388
TGTCCCG17750.011.82429711
GACCGTT907.4922154E-611.6077777
TCGCTCC1201.2821693E-711.080159
GTACAAA7000.010.8560571
GTACTGG28500.010.632271
TTAGACT3400.010.6144554
TACTGGT27250.010.5949792
TCCCGCC19800.010.5121513
GTCCCGC19900.010.45932512
GTGTCCC20150.010.41636310
CGCCCTA20150.010.37686316
CCCGCCC20350.010.32144514
ACTGGTT28300.010.2353023
Produced by FastQC (version 0.11.5)