FastQCFastQC Report
Wed 25 May 2016
SRR1294642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741311
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC585821.5658147638621864No Hit
GTACATGGGAAGCAGTGGTATCAAC510721.3650829882893991No Hit
GTATCAACGCAGAGTACTTTTTTTT430491.1506394416288837No Hit
GGTATCAACGCAGAGTACTTTTTTT268170.7167808289661031No Hit
TATCAACGCAGAGTACTTTTTTTTT256290.6850272538155743No Hit
GAGTACATGGGAAGCAGTGGTATCA242990.6494782176622045No Hit
CATGTACTCTGCGTTGATACCACTG217980.5826299925347024No Hit
GCGTTGATACCACTGCTTCCCATGT180640.4828254053191515No Hit
ACGCAGAGTACTTTTTTTTTTTTTT160630.4293414794974275No Hit
ACGCAGAGTACATGGGAAGCAGTGG148700.39745426135384093No Hit
GTATCAACGCAGAGTACATGGGAAG147210.3934717001607191No Hit
GTACTGGTTCACTATCGGTCAGTCA128530.3435426779543321No Hit
TATCAACGCAGAGTACATGGGAAGC122800.3282271909499103No Hit
ACTCTGCGTTGATACCACTGCTTCC120680.3225607280442604No Hit
GTTAATGATAGTGTGTCGAAACACA106150.28372407426166923No Hit
GGTATCAACGCAGAGTACATGGGAA104570.27950095568104333No Hit
GTACTTTTTTTTTTTTTTTTTTTTT102610.27426215035317836No Hit
GGATACCACGTGTCCCGCCCTACTC91690.24507452066935895No Hit
GCTTCCCATGTACTCTGCGTTGATA89920.24034355871511348No Hit
GAGTACTTTTTTTTTTTTTTTTTTT85170.22764747437462432No Hit
AGCGTACACGGTGGATGCCCTGGCA79620.2128131021452106No Hit
CAGTGGTATCAACGCAGAGTACATG78460.20971258470627008No Hit
ATACAGGGTGACAGCCCCGTACACA77150.2062111382881562No Hit
GTGGTATCAACGCAGAGTACATGGG73410.19621464240743416No Hit
ACTTAGATGTTTCAGTTCCCCCGGT70940.1896126785503798No Hit
ATTCCATTCCATTCCATTCCATTCC66620.1780659239501875No Hit
ATACCACTGCTTCCCATGTACTCTG65660.17549997848347812No Hit
GTACATGGGGGTTAAGCGACTAAGC65570.17525942109597412No Hit
CATGGGAAGCAGTGGTATCAACGCA61240.16368593789717026No Hit
GTGGATGCCCTGGCAGTCAGAGGCG61110.16333846611522004No Hit
GGGAAGCAGTGGTATCAACGCAGAG59970.16029140587350263No Hit
GAATGGAATGGAATGGAATGGAATG59870.16002411988738707No Hit
ACCCTGTATCGCGCGCCTTTCCAGA59380.15871441855542082No Hit
GCAGTGGTATCAACGCAGAGTACAT59130.1580462035901319No Hit
TCATTAACCTATGGATTCAGTTAAT58910.15745817442067767No Hit
GTATCAACGCAGAGTACATGGGGGT58020.15507932914424916No Hit
GTACTCTGCGTTGATACCACTGCTT57250.15302122705115934No Hit
GCAGAGTACTTTTTTTTTTTTTTTT53970.14425424670656892No Hit
CTATCGGTCAGTCAGGAGTATTTAG52950.14152792964819016No Hit
ACATGGGAAGCAGTGGTATCAACGC52250.13965692774538124No Hit
CTGCTTCCCATGTACTCTGCGTTGA50720.13556745215781313No Hit
TACCACTGCTTCCCATGTACTCTGC50460.13487250859391267No Hit
GCAGAGTACATGGGAAGCAGTGGTA50060.13380336464945042No Hit
CCCATATTCAGACAGGATACCACGT49970.13356280726194641No Hit
CCTATGGATTCAGTTAATGATAGTG49910.13340243567027707No Hit
ATCATTAACTGAATCCATAGGTTAA49280.13171853395774902No Hit
GGTATCAACGCAGAGTACATGGGGG48670.1300880894424441No Hit
GTACTTAGATGTTTCAGTTCCCCCG48390.12933968868132054No Hit
CCATGTACTCTGCGTTGATACCACT47700.12749541537712314No Hit
GTTCACTATCGGTCAGTCAGGAGTA45620.12193586686591946No Hit
GTCAGGAGTATTTAGCCTTGGAGGA45550.12174876667563857No Hit
GATACCACTGCTTCCCATGTACTCT44100.11787311987696292No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG42900.11466568804357616No Hit
GTATTTAGCCTTGGAGGATGGTCCC42730.11421130186717972No Hit
AAGCAGTGGTATCAACGCAGAGTAC42370.11324907231716368No Hit
TATTCAGACAGGATACCACGTGTCC42300.11306197212688279No Hit
CCACTGCTTCCCATGTACTCTGCGT41770.11164535640047031No Hit
ATCAACGCAGAGTACTTTTTTTTTT41670.11137807041435475No Hit
GCCCAGAGCCTGAATCAGTGTGTGT41140.10996145468794227No Hit
CTTCCCATGTACTCTGCGTTGATAC40820.10910613953237247No Hit
GCCTTGGAGGATGGTCCCCCCATAT40550.10838446736986046No Hit
ATGGGAAGCAGTGGTATCAACGCAG40110.107208409030952No Hit
GTTGATACCACTGCTTCCCATGTAC39120.10456227776840792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC601.4684592E-615.833023
CCGAACA1500.013.90903817
CCCGAAC1351.8189894E-1213.34705616
AATCGCC4050.013.11289718
ATCGCCG4100.012.95350319
AAATCGC4300.012.57106517
TACGGCC1251.4515535E-912.13928319
AGACCGT802.8106508E-511.898826
CGAACAC1800.011.59086418
GAAATCG4600.011.54505216
CGAGAAC600.00587913911.0831143
CTGTCGC1303.178502E-810.9829379
TAACGCC951.3874742E-510.98125919
TGTCGCC1053.3897231E-610.87833810
CCGTGCT700.001468169210.8783379
AACCGAT909.691018E-510.5372915
TTAACGC909.6923315E-510.53714918
GTACTGG30600.010.4115511
AGGGTGA15550.010.3454695
CCCCGTA17550.010.3211415
Produced by FastQC (version 0.11.5)