FastQCFastQC Report
Wed 25 May 2016
SRR1294641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384087
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116820.3452038910347163No Hit
GTACATGGGAAGCAGTGGTATCAAC103960.3072025039545378No Hit
CCCATGTACTCTGCGTTGATACCAC101970.30132204047945577No Hit
GGTATCAACGCAGAGTACTTTTTTT66530.19659660050111005No Hit
TATCAACGCAGAGTACTTTTTTTTT62600.18498342388951586No Hit
ATTCCATTCCATTCCATTCCATTCC50780.15005524385159127No Hit
GAGTACATGGGAAGCAGTGGTATCA45790.13530976006231518No Hit
GTACTGGTTCACTATCGGTCAGTCA39820.11766836963706903No Hit
CATGTACTCTGCGTTGATACCACTG38640.11418146164681936No Hit
GAATGGAATGGAATGGAATGGAATG38380.11341315988625587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA550.003077271612.0869474
AAATCGC1001.9179515E-611.40672217
TTAACGC600.00589113711.08002953
TAACGCT600.005892433711.0797024
GTTCTAA3150.010.8559941
CGCAGAA1803.6379788E-1110.552412
CGACTAA4250.010.51207617
AGCGACT4300.010.16803115
TCCGTAC1502.4594556E-810.1303133
TATACCG750.00265681110.1297135
ATCGCCG1054.117552E-59.95029719
GCGACTA4450.09.82572416
ACCGTGG1451.627759E-79.8239318
GTATAAT5750.09.7467941
TAGGACC1951.6552804E-109.7403984
GTACTGG12150.09.6944471
CTAGGAC2201.6370905E-119.4971693
CCGTACC1606.6766006E-89.4968874
GGACCGT1106.8678666E-59.4966076
CCGACCA800.00453165369.4964679
Produced by FastQC (version 0.11.5)