FastQCFastQC Report
Wed 25 May 2016
SRR1294641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384087
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT327860.9688285200705536No Hit
GGTATCAACGCAGAGTACTTTTTTT208820.6170645140033338No Hit
TATCAACGCAGAGTACTTTTTTTTT199120.588400948320773No Hit
ACGCAGAGTACTTTTTTTTTTTTTT116400.3439627881907291No Hit
CCCATGTACTCTGCGTTGATACCAC113500.3353932685536749No Hit
GTACATGGGAAGCAGTGGTATCAAC98860.2921319694204079No Hit
GTACTTTTTTTTTTTTTTTTTTTTT73680.21772489891660587No Hit
GAGTACTTTTTTTTTTTTTTTTTTT61190.18081686434184463No Hit
GAGTACATGGGAAGCAGTGGTATCA47890.14151527428225102No Hit
ATTCCATTCCATTCCATTCCATTCC47750.1411015733342553No Hit
GAATGGAATGGAATGGAATGGAATG44070.1302271484155106No Hit
CATGTACTCTGCGTTGATACCACTG43380.12818819374324597No Hit
GCAGAGTACTTTTTTTTTTTTTTTT39830.117697919704783No Hit
GTACTGGTTCACTATCGGTCAGTCA37780.11164015582341706No Hit
GCGTTGATACCACTGCTTCCCATGT34120.10082483104010033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG400.00520189114.2823734
AGAACCG957.180461E-813.0295335
CGCTAAG550.003042904412.1050851
GACCGTT803.694843E-410.711787
TATTCCG1855.820766E-1110.2936025
GCGCCTT3850.010.0967213
TCTGTCG1553.9281076E-89.8287318
CGCGCGC3850.09.64495310
TATCGCG3400.09.5215837
CTGCCCG1401.0277236E-69.5212999
TGTCCCG4600.09.48947811
AAATCGC1304.3505224E-69.48120517
TCGCGCG3450.09.383319
GTACTGG11700.09.3485561
GTATTAA4300.09.2899481
CGCGCCT4000.09.24431312
ACACCGT1252.6832495E-59.1407186
CCGTACA5100.09.10939417
GGACCGT1151.08418164E-49.1076016
ATCGCCG1151.1353596E-49.06911319
Produced by FastQC (version 0.11.5)