FastQCFastQC Report
Wed 25 May 2016
SRR1294640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950268
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT160660.5445606975366305No Hit
GGTATCAACGCAGAGTACTTTTTTT94020.31868291287435585No Hit
TATCAACGCAGAGTACTTTTTTTTT89100.3020064617858445No Hit
GTACTGGTTCACTATCGGTCAGTCA76180.2582138300656076No Hit
ATACAGGGTGACAGCCCCGTACACA47110.1596804086950745No Hit
GGATACCACGTGTCCCGCCCTACTC46830.1587313423729641No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40830.13839420689916984No Hit
GTTAATGATAGTGTGTCGAAACACA39610.13425898935283168No Hit
ACCCTGTATCGCGCGCCTTTCCAGA33730.11432859658851331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.014.1224881
GAAATCG2700.012.67214316
CGATCAA550.003076801312.0871110
ATCGCCG2450.012.01887419
AAATCGC2750.011.75053217
AATCGCC2700.011.61770818
TGTCCCG8800.011.22336411
CGGACAG600.00589005511.0802275
GTATCAA70200.011.0150111
CCGAATG2800.010.86331218
CGCCCTA9400.010.71739816
GTACTGG20450.010.2194381
CCGTACA14500.010.15956317
TTATCGT750.002654569210.1306654
ACAGCGT750.002655689810.130158
GTCCCGC9700.010.09164712
AGGATCG856.613703E-410.0560055
ACCGTTT856.615765E-410.0556638
TACACCG1054.120274E-59.9495925
CCGCCCT10150.09.92513415
Produced by FastQC (version 0.11.5)