##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294640_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2950268 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.803934083276502 31.0 30.0 33.0 25.0 34.0 2 29.85526535216462 31.0 30.0 34.0 25.0 34.0 3 29.83901665882557 31.0 30.0 34.0 25.0 34.0 4 33.33009780806354 35.0 33.0 37.0 26.0 37.0 5 33.87264546814052 35.0 35.0 37.0 28.0 37.0 6 34.00657194532835 37.0 35.0 37.0 29.0 37.0 7 33.92279142098277 37.0 35.0 37.0 28.0 37.0 8 33.73074107165858 37.0 35.0 37.0 27.0 37.0 9 34.770775061791 39.0 35.0 39.0 26.0 39.0 10 34.92434856765555 38.0 35.0 39.0 27.0 39.0 11 35.15469069250658 39.0 35.0 39.0 27.0 39.0 12 34.9642140985158 38.0 35.0 39.0 27.0 39.0 13 34.79403057620528 38.0 35.0 39.0 27.0 39.0 14 35.64587522218321 39.0 35.0 41.0 25.0 41.0 15 35.662263224900244 39.0 34.0 41.0 26.0 41.0 16 35.809269530768056 39.0 35.0 41.0 27.0 41.0 17 35.83789676056548 39.0 35.0 41.0 27.0 41.0 18 35.725556796874045 39.0 34.0 41.0 26.0 41.0 19 36.02838386207626 39.0 35.0 41.0 27.0 41.0 20 36.151449631016575 39.0 35.0 41.0 27.0 41.0 21 36.214223589179014 39.0 35.0 41.0 27.0 41.0 22 36.24054424886146 39.0 35.0 41.0 27.0 41.0 23 36.24299182311573 39.0 35.0 41.0 27.0 41.0 24 36.26577043170315 39.0 35.0 41.0 27.0 41.0 25 35.15894250962963 38.0 33.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0071402273254648 1101 2 0.9574136381550389 1101 3 1.2345689572596612 1101 4 1.157582819544693 1101 5 0.7973007867026283 1101 6 0.8371744349410406 1101 7 0.7512977328647779 1101 8 0.9027560447854768 1101 9 1.2395455944136486 1101 10 1.1383011769505416 1101 11 0.8636857971515468 1101 12 0.9850757194954411 1101 13 1.0924775039449202 1101 14 1.2681695630203365 1101 15 1.3577361493936166 1101 16 1.2142524433972923 1101 17 1.2149353588138112 1101 18 1.2657389747314411 1101 19 1.117246813760751 1101 20 1.0955036484145069 1101 21 0.9792275296870727 1101 22 0.9686103518463369 1101 23 1.0608232666779145 1101 24 0.943800461158034 1101 25 1.0710364466084172 1102 1 0.9228428429505939 1102 2 0.9161780305822198 1102 3 1.198029414146216 1102 4 1.0396080592944656 1102 5 0.8135456646725245 1102 6 0.7254693754821275 1102 7 0.676621059917359 1102 8 0.7204567359489857 1102 9 1.1248324938832042 1102 10 0.9088512171041643 1102 11 0.828143995292919 1102 12 0.9923932338206498 1102 13 1.058223658183124 1102 14 1.279335553519637 1102 15 1.3244272901640386 1102 16 1.239818237797195 1102 17 1.262441944531993 1102 18 1.403370808847093 1102 19 1.1664548350843873 1102 20 0.36560980803292153 1102 21 0.5846571402614202 1102 22 0.7435088884201164 1102 23 0.8176872883786217 1102 24 0.9919040182952941 1102 25 0.9750205522364013 1103 1 0.7662537949046602 1103 2 0.7232362620481432 1103 3 1.0113230442312187 1103 4 0.8691717702690269 1103 5 0.6215447855546969 1103 6 0.5782256842088884 1103 7 0.4922314555802956 1103 8 0.6316143115135446 1103 9 0.7068797915576113 1103 10 0.777859349574733 1103 11 0.6594590363746491 1103 12 0.862818675768132 1103 13 0.7683044405349264 1103 14 0.704500459060192 1103 15 0.9983063597496198 1103 16 0.9338700975507805 1103 17 0.9724976903143272 1103 18 1.047840233803754 1103 19 0.8828881581907737 1103 20 0.01376761788175429 1103 21 0.4252117160413462 1103 22 -0.13819382533590385 1103 23 0.3781169587945783 1103 24 0.541475196095611 1103 25 0.6155544113587155 1104 1 0.37868838112968817 1104 2 0.32350857411245926 1104 3 0.6272250254697127 1104 4 0.457298229621081 1104 5 0.28810736740149423 1104 6 0.17597974245965275 1104 7 0.1680513820510967 1104 8 0.3161833511258152 1104 9 0.5159930163643054 1104 10 0.5348969202745479 1104 11 0.3705638402502345 1104 12 0.4644121427479746 1104 13 0.3436723295963162 1104 14 0.7441652948279582 1104 15 0.6757385205699222 1104 16 0.5341439529843157 1104 17 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2207 8 0.4528073249602258 2207 9 -1.4120679657470845 2207 10 -0.14018383454766337 2207 11 0.1661136845739719 2207 12 0.5561959974397936 2207 13 0.7026255434984279 2207 14 0.9056842889178895 2207 15 0.8951725006009568 2207 16 0.8560637561512294 2207 17 0.8187774585667071 2207 18 0.8529406532939277 2207 19 -0.31115741643961314 2207 20 0.28303635062470534 2207 21 -0.3573314650552817 2207 22 0.21596022840978435 2207 23 -0.6094911295532626 2207 24 -1.4604581102665222 2207 25 -0.16682233748252884 2208 1 0.5536312383978625 2208 2 0.2268572546845462 2208 3 0.6272260223025761 2208 4 0.5591047129194919 2208 5 0.36422501213493064 2208 6 0.2838440645070719 2208 7 0.31710113546027685 2208 8 0.6021613418536731 2208 9 -0.04822395347910913 2208 10 0.11689217637543692 2208 11 0.317820502098499 2208 12 0.2669553026204312 2208 13 0.5069239607993694 2208 14 0.7380847567706112 2208 15 0.6967571117306619 2208 16 0.4652330680764152 2208 17 0.46698314336622104 2208 18 0.6934980105481543 2208 19 -1.3720650487569657 2208 20 -0.06860121288696774 2208 21 -0.10840831238687088 2208 22 0.1981666101618984 2208 23 0.4607971448375352 2208 24 0.5330070738896069 2208 25 0.6164055205311101 2209 1 0.060940517073809986 2209 2 -1.6051464006503338 2209 3 -0.3356498573560103 2209 4 -0.00255429120506534 2209 5 0.022391384445128892 2209 6 0.08774861703619052 2209 7 0.07447143447330973 2209 8 0.24736090591004256 2209 9 -2.068807308971664 2209 10 -0.4230391469874064 2209 11 -0.1511824507400803 2209 12 0.1915288156743955 2209 13 0.360016469342483 2209 14 0.4881947324855389 2209 15 0.5761960670496507 2209 16 0.48299573775495475 2209 17 0.35812131042337114 2209 18 0.5486863977975034 2209 19 0.3083104791184397 2209 20 0.23965121923225752 2209 21 0.27353903088770437 2209 22 0.2956140286083908 2209 23 0.20442269986578765 2209 24 0.3289653927993825 2209 25 0.34064041217921925 2210 1 0.036139806120218054 2210 2 0.041886474352736514 2210 3 -0.023142956528069192 2210 4 0.11396553182747482 2210 5 -0.08183724226172728 2210 6 -0.02698030418519437 2210 7 -0.027715260017565413 2210 8 0.043851206203981974 2210 9 0.022108125622743557 2210 10 -1.9421816410647708 2210 11 -0.8814606039399138 2210 12 -2.56424080452463 2210 13 -0.9918866014500409 2210 14 -0.7271501214571714 2210 15 -0.8710790992896804 2210 16 -0.3372335162639004 2210 17 -0.06693745387233463 2210 18 0.16060389993408108 2210 19 0.011451395411100407 2210 20 0.03733903594098109 2210 21 0.0599946626893626 2210 22 -0.036300680194493395 2210 23 -0.12884883513972767 2210 24 0.031822777800769586 2210 25 -0.02103782165238499 2211 1 -0.33350466692767355 2211 2 -0.08111034880965562 2211 3 -1.5297295958437296 2211 4 -0.7950424968046264 2211 5 -0.5028831830106668 2211 6 -0.5054634645394671 2211 7 -0.4374076989524198 2211 8 -0.17339315135818367 2211 9 0.1037599733808463 2211 10 -0.019545430429730004 2211 11 -0.2794783511329797 2211 12 -0.2960787921764947 2211 13 -0.36238325270682026 2211 14 -0.8903109842290107 2211 15 -0.4083849051481039 2211 16 -0.1843251699153683 2211 17 -0.19931776495970865 2211 18 -0.11955883956256486 2211 19 -0.06489876329467137 2211 20 -0.032634853460251634 2211 21 -0.2275850896761753 2211 22 -0.26239878960290497 2211 23 -0.5000109231100041 2211 24 -0.3543827531556616 2211 25 -0.09034363382626509 2212 1 -0.42335615230921064 2212 2 -0.5031194630785727 2212 3 -2.4834687266673576 2212 4 -1.556641207730781 2212 5 -0.7515900964022819 2212 6 -0.5324629505291512 2212 7 -0.4562290314158872 2212 8 -0.4210977564104539 2212 9 -1.040029292276344 2212 10 -0.3299459371911766 2212 11 -0.5774085770574615 2212 12 -0.20730657546937437 2212 13 -0.6017541949319636 2212 14 -0.261965680857017 2212 15 -0.059124371584687196 2212 16 -1.289290417734712 2212 17 -1.5489620999494562 2212 18 -0.3984040203157946 2212 19 -0.27038073974904364 2212 20 -0.36145900544914156 2212 21 -0.19464571323846513 2212 22 -0.49787105540460175 2212 23 -0.23932232110037432 2212 24 -0.25254393069115366 2212 25 -0.08982003289219875 2213 1 -3.101861605813241 2213 2 -1.422807766958087 2213 3 -2.5960607173333123 2213 4 -1.1529386222387288 2213 5 -0.9901916738965397 2213 6 -0.7521692269720148 2213 7 -0.39917226149465534 2213 8 -0.31873505839261895 2213 9 -0.3782580486699416 2213 10 -0.2829753552088903 2213 11 -0.6347232459568133 2213 12 -0.352754658505539 2213 13 -0.5876819883322355 2213 14 -0.24337951154816295 2213 15 -0.5185506705590086 2213 16 -1.8206453261805748 2213 17 -1.1829864637316732 2213 18 -0.3699817995060499 2213 19 -0.23848857220676223 2213 20 -0.24764035080509927 2213 21 -0.07032068892801391 2213 22 -0.07730795892189235 2213 23 -0.06681575855767363 2213 24 -0.025894107823184243 2213 25 -0.20515466594870446 2214 1 -0.6738638128434218 2214 2 -0.5668779294095074 2214 3 -6.269469884577969 2214 4 -1.8219288087531993 2214 5 -1.1340473698936933 2214 6 -0.5259261226343668 2214 7 -0.32638442577115967 2214 8 -0.3600941176137198 2214 9 -0.03519764373488954 2214 10 0.07463764730117362 2214 11 -0.6045892022702404 2214 12 -0.28430763119695257 2214 13 -5.276848935306003 2214 14 -3.966660340270657 2214 15 -2.0439154957110333 2214 16 -2.740095367028836 2214 17 -3.459402724975554 2214 18 -1.113818379881188 2214 19 -0.6073246221049331 2214 20 -0.2344642256265601 2214 21 -0.3767414037181993 2214 22 -0.25547816459257433 2214 23 -0.14938817266084925 2214 24 -0.015285798665765071 2214 25 0.026432878464518694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1131.0 3 167.0 4 146.0 5 295.0 6 51.0 7 50.0 8 97.0 9 275.0 10 662.0 11 1763.0 12 4175.0 13 7625.0 14 12543.0 15 18368.0 16 22985.0 17 25979.0 18 26675.0 19 26256.0 20 25082.0 21 24092.0 22 23557.0 23 23067.0 24 23878.0 25 25138.0 26 27179.0 27 30385.0 28 35527.0 29 44010.0 30 55127.0 31 70328.0 32 93501.0 33 126342.0 34 172344.0 35 241399.0 36 341986.0 37 519752.0 38 898331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31252720646787 20.754001280464035 13.141483132922957 22.79198838014514 2 15.676096565747825 21.92151404717866 39.834727504200146 22.56766188287337 3 18.647281621490365 27.40263501680122 29.525136709132077 24.424946652576345 4 12.16022089329934 18.721704150295345 37.63243729385361 31.485637662551706 5 12.7498220492131 39.668053535464175 33.91163806624212 13.670486349080607 6 29.039874366575543 39.44146717046665 17.95160535366623 13.567053109291582 7 25.77104127923679 35.24135093710688 21.583830865935667 17.403776917720663 8 24.208376759855206 37.12342719114713 20.87277897293746 17.795417076060204 9 25.056515101615258 18.28796466606316 21.064251014219252 35.59126921810233 10 15.254850112709706 31.083458095256834 32.08577062845413 21.57592116357933 11 31.430835042389223 27.283382889453616 23.013597720068006 18.27218434808915 12 20.909026468955286 28.876642248377383 29.96534138179322 20.248989900874115 13 26.715370305364356 24.93616126466663 26.42698100185013 21.92148742811889 14 20.641169395949117 25.236145508368285 27.67967917449296 26.443005921189638 15 22.763865301954034 31.29128420218613 24.484985142350897 21.45986535350894 16 20.99276809646246 31.61369160813617 25.568779394552347 21.824760900849018 17 19.928367442459926 30.432271281178135 27.410645846193503 22.22871543016843 18 20.285629715320734 29.48439603500632 29.112940948043807 21.117033301629135 19 21.16899609323857 29.437844232896982 29.280495159455427 20.112664514409023 20 22.49356005555565 27.86237936233103 29.029760259395367 20.614300322717956 21 23.013434828262657 28.186990072091973 28.224602978643738 20.574972121001636 22 22.45318677263465 28.103843826569236 28.465881494224494 20.977087906571626 23 22.231530762728998 28.310814202586705 28.50495945471097 20.952695579973327 24 21.985281771479066 27.931086533618043 28.56716909002407 21.51646260487883 25 21.77224266401828 28.553955487536836 28.702011527002163 20.97179032144272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3983.0 1 3983.0 2 4062.0 3 4141.0 4 4141.0 5 4141.0 6 6684.0 7 9227.0 8 9227.0 9 9227.0 10 12612.5 11 15998.0 12 15998.0 13 15998.0 14 23689.0 15 31380.0 16 31380.0 17 31380.0 18 48410.0 19 65440.0 20 65440.0 21 65440.0 22 92848.0 23 120256.0 24 120256.0 25 120256.0 26 155502.5 27 190749.0 28 190749.0 29 190749.0 30 222779.5 31 254810.0 32 254810.0 33 254810.0 34 274492.0 35 294174.0 36 294174.0 37 294174.0 38 297895.5 39 301617.0 40 301617.0 41 301617.0 42 310352.0 43 319087.0 44 319087.0 45 319087.0 46 332307.0 47 345527.0 48 345527.0 49 345527.0 50 326839.5 51 308152.0 52 308152.0 53 308152.0 54 278188.5 55 248225.0 56 248225.0 57 248225.0 58 214835.0 59 181445.0 60 181445.0 61 181445.0 62 155941.5 63 130438.0 64 130438.0 65 130438.0 66 99259.0 67 68080.0 68 68080.0 69 68080.0 70 51904.5 71 35729.0 72 35729.0 73 35729.0 74 25147.5 75 14566.0 76 14566.0 77 14566.0 78 9830.0 79 5094.0 80 5094.0 81 5094.0 82 3316.0 83 1538.0 84 1538.0 85 1538.0 86 976.0 87 414.0 88 414.0 89 414.0 90 278.5 91 143.0 92 143.0 93 143.0 94 88.5 95 34.0 96 34.0 97 34.0 98 27.5 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09826225956421587 2 0.052944342683444355 3 0.05667281752030663 4 0.05131737184554081 5 0.04809732539552339 6 0.06806161338563141 7 0.06389250061350359 8 0.06104530164717239 9 0.06579063325772438 10 0.06731591841825896 11 0.0596217021640068 12 0.06084193029243445 13 0.06179099661454485 14 0.06351965312981736 15 0.06626516641877958 16 0.061180882550331016 17 0.06399418629087256 18 0.14527493773447023 19 0.06934963196563837 20 0.0634857579040277 21 0.06141814913085863 22 0.06629906164456924 23 0.06643464254772787 24 0.07534908693040768 25 0.06982416512669357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2950268.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.45437721606104 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58482305369483 46.12757336075721 2 13.704855348847895 16.29627279203909 3 4.462432710479717 7.959334730104524 4 1.8307933245608303 4.353947076923443 5 0.8270452839749501 2.4585731144105507 6 0.464376954859619 1.6565545586801722 7 0.2696832014790227 1.1223692752698162 8 0.16889647736855595 0.8033307900747234 9 0.11139236176351787 0.5960487145748513 >10 0.4968441150902723 5.218884921370216 >50 0.036734311822349194 1.5245598913457514 >100 0.033679893588071864 4.456541962080697 >500 0.0046262808058656225 1.9698238925458342 >1k 0.003585367624290378 4.01229606451646 >5k 1.7348553020759893E-4 0.8915143827531669 >10k+ 5.782851006919965E-5 0.5523744725535048 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16066 0.5445606975366305 No Hit GGTATCAACGCAGAGTACTTTTTTT 9402 0.31868291287435585 No Hit TATCAACGCAGAGTACTTTTTTTTT 8910 0.3020064617858445 No Hit GTACTGGTTCACTATCGGTCAGTCA 7618 0.2582138300656076 No Hit ATACAGGGTGACAGCCCCGTACACA 4711 0.1596804086950745 No Hit GGATACCACGTGTCCCGCCCTACTC 4683 0.1587313423729641 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4083 0.13839420689916984 No Hit GTTAATGATAGTGTGTCGAAACACA 3961 0.13425898935283168 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 3373 0.11432859658851331 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.389522578965707E-5 0.0 3 0.0 0.0 0.0 3.389522578965707E-5 0.0 4 0.0 0.0 0.0 6.779045157931414E-5 0.0 5 0.0 0.0 0.0 1.0168567736897123E-4 0.0 6 0.0 0.0 0.0 1.0168567736897123E-4 0.0 7 0.0 0.0 0.0 1.0168567736897123E-4 0.0 8 0.0 0.0 0.0 2.0337135473794246E-4 0.0 9 0.0 0.0 0.0 4.40637935265542E-4 0.0 10 0.0 0.0 0.0 5.084283868448562E-4 0.0 11 0.0 0.0 0.0 5.084283868448562E-4 0.0 12 0.0 0.0 0.0 6.440092900034844E-4 0.0 13 0.0 0.0 0.0 6.779045157931415E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 14.122488 1 GAAATCG 270 0.0 12.672143 16 CGATCAA 55 0.0030768013 12.08711 10 ATCGCCG 245 0.0 12.018874 19 AAATCGC 275 0.0 11.750532 17 AATCGCC 270 0.0 11.617708 18 TGTCCCG 880 0.0 11.223364 11 CGGACAG 60 0.005890055 11.080227 5 GTATCAA 7020 0.0 11.015011 1 CCGAATG 280 0.0 10.863312 18 CGCCCTA 940 0.0 10.717398 16 GTACTGG 2045 0.0 10.219438 1 CCGTACA 1450 0.0 10.159563 17 TTATCGT 75 0.0026545692 10.130665 4 ACAGCGT 75 0.0026556898 10.13015 8 GTCCCGC 970 0.0 10.091647 12 AGGATCG 85 6.613703E-4 10.056005 5 ACCGTTT 85 6.615765E-4 10.055663 8 TACACCG 105 4.120274E-5 9.949592 5 CCGCCCT 1015 0.0 9.925134 15 >>END_MODULE