FastQCFastQC Report
Wed 25 May 2016
SRR1294640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950268
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT449711.5243021989866685No Hit
GGTATCAACGCAGAGTACTTTTTTT285750.9685560769394509No Hit
TATCAACGCAGAGTACTTTTTTTTT264760.8974099980069608No Hit
ACGCAGAGTACTTTTTTTTTTTTTT150260.5093096627153872No Hit
GTACTTTTTTTTTTTTTTTTTTTTT87870.2978373490137167No Hit
GAGTACTTTTTTTTTTTTTTTTTTT77810.2637387518693217No Hit
GTACTGGTTCACTATCGGTCAGTCA71460.24221528349288945No Hit
GCAGAGTACTTTTTTTTTTTTTTTT54090.18333927629625513No Hit
ATACAGGGTGACAGCCCCGTACACA48880.1656798636598438No Hit
GGATACCACGTGTCCCGCCCTACTC47190.15995157050139175No Hit
ATCAACGCAGAGTACTTTTTTTTTT43500.14744423218500827No Hit
GTTAATGATAGTGTGTCGAAACACA39340.13334381825651093No Hit
ACCCTGTATCGCGCGCCTTTCCAGA35460.12019247065012399No Hit
GGTATCAACGCAGAGTACATGGGGG31350.10626153285057494No Hit
GTGGTATCAACGCAGAGTACTTTTT30060.10188904872370917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA250.00608182818.97368211
GGTATCA55900.015.6665081
CCTAGAC1657.2759576E-1211.51384453
CTATACT3100.011.0584194
TGTCCCG10350.010.63259511
GTATCAA126350.010.5811871
CGCGCCT8950.010.3818412
CTAAGAC2300.010.3249143
GCGCCTT9200.010.30584113
TATCGCG9000.010.1573637
TCGCGCG9200.010.1423539
GTGGTAT11600.010.0825761
CGCCTTT10400.010.02837814
TGGACCG1054.0020714E-59.9759825
TCTATAC3050.09.9660823
GTATAGA3450.09.9221951
CCCGAAC1151.0511629E-59.89377516
CGCGCGC9550.09.77064310
TGTATCG10450.09.75033955
ATCGCGC9700.09.7188688
Produced by FastQC (version 0.11.5)