##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294640_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2950268 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.252740429005094 31.0 31.0 34.0 28.0 34.0 2 31.355797168257258 31.0 31.0 34.0 28.0 34.0 3 31.438668622647164 31.0 31.0 34.0 28.0 34.0 4 35.01500134902999 37.0 35.0 37.0 32.0 37.0 5 35.20234737996684 37.0 35.0 37.0 32.0 37.0 6 34.73676221956785 37.0 35.0 37.0 32.0 37.0 7 34.59444735190159 37.0 35.0 37.0 31.0 37.0 8 34.56905576035804 37.0 35.0 37.0 31.0 37.0 9 35.97154868642442 39.0 35.0 39.0 30.0 39.0 10 35.763642828380334 39.0 35.0 39.0 30.0 39.0 11 35.92975316140771 39.0 35.0 39.0 30.0 39.0 12 35.88886501158539 39.0 35.0 39.0 30.0 39.0 13 35.72785285946904 39.0 35.0 39.0 30.0 39.0 14 36.70435058781101 39.0 36.0 41.0 29.0 41.0 15 36.7386254401295 39.0 36.0 41.0 30.0 41.0 16 36.814092143493404 39.0 36.0 41.0 30.0 41.0 17 36.91090199263253 39.0 36.0 41.0 30.0 41.0 18 36.93118760736313 40.0 36.0 41.0 30.0 41.0 19 36.969614624840865 40.0 36.0 41.0 30.0 41.0 20 37.04181721796122 40.0 36.0 41.0 30.0 41.0 21 36.97272315599803 40.0 36.0 41.0 30.0 41.0 22 36.91700516698822 40.0 36.0 41.0 30.0 41.0 23 36.92051196704842 40.0 36.0 41.0 30.0 41.0 24 36.816931885510066 39.0 36.0 41.0 30.0 41.0 25 35.67073737029992 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4140853047186184 1101 2 0.45105846432459273 1101 3 0.2914460966792731 1101 4 0.28468759511463304 1101 5 0.2930811903284578 1101 6 0.34172530540750756 1101 7 0.4184795962633956 1101 8 0.4208918165514035 1101 9 0.6003539676795171 1101 10 0.7008592599803336 1101 11 0.5806560830130962 1101 12 0.7627884639469045 1101 13 0.6663336480758417 1101 14 0.8162191413816444 1101 15 0.7496865014645024 1101 16 0.8959417129244684 1101 17 0.8634715696481123 1101 18 0.8464708924508244 1101 19 0.9772760132228484 1101 20 0.9043199723314004 1101 21 1.116726980945863 1101 22 1.038474309505105 1101 23 1.0965235190546991 1101 24 1.1907774105635127 1101 25 1.0414839047775502 1102 1 0.3896152246297966 1102 2 0.38597376233832037 1102 3 0.3126895804172136 1102 4 0.28144828909087494 1102 5 0.30503288130891804 1102 6 0.34456492734647526 1102 7 0.3915469052858711 1102 8 0.4810307313599367 1102 9 0.5949573383869549 1102 10 0.7057091817360117 1102 11 0.6577274116133225 1102 12 0.686819428031157 1102 13 0.6757745080519086 1102 14 0.7554361207892981 1102 15 0.7912263747739772 1102 16 0.7846787457050723 1102 17 0.8068492744293678 1102 18 0.7559285240856752 1102 19 0.916394061558691 1102 20 0.8228593997165703 1102 21 1.128987727740693 1102 22 1.0306894751977254 1102 23 0.9043671244222935 1102 24 1.0222512995943944 1102 25 1.0051107370301793 1103 1 0.3391614919894579 1103 2 0.2951849811178917 1103 3 0.2147271913987474 1103 4 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0.1682401707865715 2207 2 0.1750674158287282 2207 3 0.17510069867809008 2207 4 0.2083528710909448 2207 5 0.12587702802731826 2207 6 0.2368144924925204 2207 7 0.22451398701527125 2207 8 0.18198996984926197 2207 9 0.3150193323266137 2207 10 0.548170349801687 2207 11 0.3722034432276118 2207 12 0.309742544737297 2207 13 0.32290810848601126 2207 14 0.35138927538694276 2207 15 0.3184417866588731 2207 16 0.3813828728029165 2207 17 0.4937739764889315 2207 18 0.3494332022249367 2207 19 0.5285482954946588 2207 20 0.475669341384652 2207 21 0.7481336170961441 2207 22 0.6692368716830259 2207 23 0.5705494961867288 2207 24 0.7684086832368209 2207 25 0.6905004214004506 2208 1 0.15549937717687357 2208 2 0.1419135211879805 2208 3 0.12008946294583112 2208 4 0.13477251162269255 2208 5 0.09565579269762736 2208 6 0.25645690906916485 2208 7 0.26127033780109343 2208 8 0.15816510226017755 2208 9 0.3168792163091183 2208 10 0.36848048226898555 2208 11 -0.008719612039577385 2208 12 -0.1913853592477608 2208 13 0.09730143587031392 2208 14 -0.3698806731380344 2208 15 0.07717702306030105 2208 16 0.2763208092120095 2208 17 0.23697224697441754 2208 18 0.2934720594296749 2208 19 0.4998958027396938 2208 20 0.3965665831487044 2208 21 0.6949729773662199 2208 22 0.7339929418138738 2208 23 0.6703784775066666 2208 24 0.7200199135542675 2208 25 0.6970925993740593 2209 1 -0.03515394437863151 2209 2 -0.09723114543124822 2209 3 0.022607374461259155 2209 4 -0.5136398627987404 2209 5 -0.11969404564262476 2209 6 0.062164250075205985 2209 7 0.10068086154873157 2209 8 0.15157618993096378 2209 9 0.22767657903208516 2209 10 0.2905048379707367 2209 11 0.2808230377250922 2209 12 0.2448536050597525 2209 13 0.2903210410278021 2209 14 0.29630860279854687 2209 15 -0.2651220819925939 2209 16 -0.19953659206532848 2209 17 0.10633805814362773 2209 18 -0.2489670098510146 2209 19 0.20026866615847183 2209 20 0.23450154365535525 2209 21 0.426487988476147 2209 22 0.5713188760189141 2209 23 0.5634980800424998 2209 24 0.627892740858421 2209 25 0.629281001069252 2210 1 -0.2515776640588818 2210 2 -0.18196760970971226 2210 3 -0.14114500205055336 2210 4 -0.08948204102874513 2210 5 -0.11863706717954159 2210 6 -0.6526740019104267 2210 7 -0.2920177390650096 2210 8 -0.41501366445925214 2210 9 -0.18593822975524432 2210 10 -0.010412764229506877 2210 11 -0.02145458532218214 2210 12 -0.10135119699241812 2210 13 0.11161155063695105 2210 14 0.07785818486777174 2210 15 -0.06677260426414477 2210 16 -0.06166542916987794 2210 17 -0.9134317215235939 2210 18 -1.5757302357448424 2210 19 -0.4630323678238355 2210 20 -0.20855906825369885 2210 21 -0.36390938568456477 2210 22 -0.27957735374624804 2210 23 -0.3462536908069751 2210 24 0.07129953605188177 2210 25 0.04135453914321374 2211 1 -0.26795709816899915 2211 2 -0.18649922147882592 2211 3 -0.12253177488366163 2211 4 -0.027763867918068286 2211 5 -0.045569366636463826 2211 6 -0.0736267080206332 2211 7 -0.10016077891275899 2211 8 -0.1338021781913099 2211 9 -0.026299104700669318 2211 10 0.08306899393024025 2211 11 -0.2598950825946389 2211 12 -0.16200943517515753 2211 13 -0.08842335314517413 2211 14 -0.15185917071803345 2211 15 -0.3517363696085951 2211 16 -0.2859014863175702 2211 17 -0.42539654312803066 2211 18 -0.40569518986496433 2211 19 -0.19826877847371804 2211 20 -0.2887552851105539 2211 21 -0.015563433507921332 2211 22 -0.11574128163746167 2211 23 -0.7229629811745966 2211 24 -0.14912092413034372 2211 25 -0.7976581220935088 2212 1 -0.1786329793648349 2212 2 -0.2962497193674736 2212 3 -0.14658470382455846 2212 4 -0.06147596612981232 2212 5 -0.09129569097790835 2212 6 -0.0718413812263421 2212 7 -0.10821731392782397 2212 8 -0.3037999682293986 2212 9 -0.011370859033867475 2212 10 -0.480340850214489 2212 11 -0.21944277705598836 2212 12 -0.24738109946240883 2212 13 -0.09931600595145085 2212 14 -0.22214937472646312 2212 15 -0.09317159042356593 2212 16 -0.3297786444430528 2212 17 -0.4780907197018678 2212 18 -0.21924665750091066 2212 19 -0.37745185510785006 2212 20 -0.5520265888845302 2212 21 -0.024024010282090558 2212 22 -0.391541469072358 2212 23 -0.2623051279960862 2212 24 -0.47887007109445534 2212 25 -0.21071830272200032 2213 1 -0.21015217968804834 2213 2 -0.23594574195153584 2213 3 -0.1250145227911652 2213 4 -0.07551748786529089 2213 5 -0.11874348469964247 2213 6 -0.32364840922920024 2213 7 -0.44585958758860045 2213 8 -0.3590991164321764 2213 9 -0.29768758308853904 2213 10 -0.381510655869711 2213 11 0.005648419091158985 2213 12 -0.01958406449700334 2213 13 0.10487012185977562 2213 14 -0.5856820939241558 2213 15 -0.19779209171196044 2213 16 -0.3770773535600327 2213 17 -0.2670740132544651 2213 18 -0.25411744865168373 2213 19 -0.3240570862927328 2213 20 -0.5540183653409088 2213 21 -0.19051076866728067 2213 22 -0.42621244330949537 2213 23 -0.5558162655059178 2213 24 -0.7872861987668713 2213 25 -0.4829882995731509 2214 1 -0.2509903566604521 2214 2 -0.11514269885275752 2214 3 -0.1056688736586544 2214 4 0.03896792826205342 2214 5 0.006132514388369259 2214 6 0.011820055437617327 2214 7 -0.08904552951241129 2214 8 0.049816389996806265 2214 9 -0.03034626043387334 2214 10 0.05503996334913097 2214 11 0.236579340657876 2214 12 -0.13060621949062323 2214 13 -0.16172668791019618 2214 14 -0.08511382240015308 2214 15 0.18238761604659004 2214 16 -0.07642306173693925 2214 17 -0.23108325788417972 2214 18 -0.04267609692380603 2214 19 -0.18462326174081056 2214 20 -0.07524682328650556 2214 21 -0.30333285505869867 2214 22 -0.596866482348787 2214 23 -0.7486348313754476 2214 24 -0.3152630002251229 2214 25 -0.6819295229102238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 38.0 10 70.0 11 217.0 12 499.0 13 1443.0 14 2991.0 15 5497.0 16 8549.0 17 11219.0 18 13177.0 19 14448.0 20 15181.0 21 15533.0 22 16709.0 23 17632.0 24 19430.0 25 21836.0 26 24404.0 27 27283.0 28 32546.0 29 40482.0 30 51746.0 31 68165.0 32 90937.0 33 123048.0 34 169139.0 35 234609.0 36 334017.0 37 524953.0 38 1064458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85527198357625 20.512892718051162 12.461869439876448 24.16996585849614 2 15.462900319564188 21.979664220335238 38.90494016136839 23.65249529873218 3 18.101745743907106 27.255183658286676 30.24945841109712 24.393612186709106 4 11.927035309432119 18.317005206920868 37.89109535173835 31.86486413190866 5 12.574716517117798 38.819534120839464 34.122670188893665 14.483079173149077 6 29.12253914184903 39.40142735874898 17.353609620229822 14.12242387917217 7 25.50046024203815 35.49362865672047 21.44453278228602 17.561378318955363 8 25.02371499961012 36.0029292008096 20.6123860442567 18.360969755323588 9 25.74856834894493 16.317991773620335 21.608397889681097 36.32504198775364 10 15.582886969020429 30.253104813860894 32.16443220792019 21.999576009198492 11 31.84820916485126 25.986569541913457 23.175866825533948 18.98935446770134 12 21.745852098838498 27.93405388805675 30.52388850078316 19.796205512321592 13 27.73093834187267 23.508101636868243 27.083742900644957 21.677217120614127 14 21.25565547976501 23.928628082634745 28.840675796783717 25.975040640816534 15 23.11930318487789 29.787070333327343 25.9347513730318 21.158875108762963 16 20.611152501032112 30.693612974034167 26.784807586580445 21.91042693835327 17 19.578465271109362 29.321244483973825 28.51491068435501 22.585379560561808 18 19.754516301461194 27.74792162174346 31.31977993781559 21.177782138979754 19 20.569212971515284 27.76009753752988 32.148371205277925 19.522318285676903 20 21.720937516100253 26.305035752732636 31.928430811615506 20.045595919551605 21 22.11184776934914 26.725915682007688 31.14718702758401 20.01504952105916 22 21.528783367500786 26.735320197845823 31.36277293286909 20.373123501784303 23 21.475494574216214 26.936122687284026 31.30051029314316 20.2878724453566 24 21.185913003806096 26.63094560594007 31.311674502680603 20.871466887573227 25 21.050687020520282 27.112473277262616 31.51301791775007 20.323821784467036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2080.0 1 2080.0 2 2566.5 3 3053.0 4 3053.0 5 3053.0 6 7968.0 7 12883.0 8 12883.0 9 12883.0 10 15242.0 11 17601.0 12 17601.0 13 17601.0 14 21591.0 15 25581.0 16 25581.0 17 25581.0 18 42816.0 19 60051.0 20 60051.0 21 60051.0 22 90702.5 23 121354.0 24 121354.0 25 121354.0 26 160778.0 27 200202.0 28 200202.0 29 200202.0 30 236016.0 31 271830.0 32 271830.0 33 271830.0 34 286224.0 35 300618.0 36 300618.0 37 300618.0 38 296809.5 39 293001.0 40 293001.0 41 293001.0 42 301693.0 43 310385.0 44 310385.0 45 310385.0 46 324987.5 47 339590.0 48 339590.0 49 339590.0 50 322369.0 51 305148.0 52 305148.0 53 305148.0 54 276281.5 55 247415.0 56 247415.0 57 247415.0 58 214352.0 59 181289.0 60 181289.0 61 181289.0 62 156846.5 63 132404.0 64 132404.0 65 132404.0 66 100393.0 67 68382.0 68 68382.0 69 68382.0 70 52241.0 71 36100.0 72 36100.0 73 36100.0 74 25254.0 75 14408.0 76 14408.0 77 14408.0 78 9697.0 79 4986.0 80 4986.0 81 4986.0 82 3185.0 83 1384.0 84 1384.0 85 1384.0 86 879.5 87 375.0 88 375.0 89 375.0 90 244.5 91 114.0 92 114.0 93 114.0 94 69.5 95 25.0 96 25.0 97 25.0 98 17.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09588959375893986 2 0.0 3 0.13032714316123145 4 0.011795538574800661 5 0.02098114476379773 6 0.016405289282194025 7 0.024845200503818637 8 0.022303058569594355 9 1.0168567736897122E-4 10 0.39064247722579776 11 0.06423145287140015 12 0.00138970425737594 13 0.0 14 0.0010846472252690263 15 0.0015252851605345684 16 2.0337135473794243E-4 17 3.389522578965707E-5 18 3.7284748368622783E-4 19 6.440092900034844E-4 20 1.355809031586283E-4 21 2.711618063172566E-4 22 0.003118360772648451 23 1.0168567736897122E-4 24 3.389522578965707E-5 25 0.00220318967632771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2950268.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.07343945865162 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62511291756734 41.64935077819338 2 14.862975590733555 16.371103727433624 3 4.860508679267753 8.0305479145771 4 1.9913707491438595 4.386865455708163 5 0.9177764585785138 2.527255311404973 6 0.4909558430801808 1.6223176140448565 7 0.29281657818685203 1.1288491263884188 8 0.17310130815029875 0.7626627531703101 9 0.13215048219654546 0.6550183422614758 >10 0.5653538273614596 5.452614759004608 >50 0.040619849949252995 1.5480247174052681 >100 0.03712379139268804 4.554552744761321 >500 0.005471075745347233 2.0804152112124545 >1k 0.004165478124159974 4.294023562073961 >5k 2.4868526114387904E-4 0.9971680743025275 >10k+ 2.4868526114387904E-4 3.939229908057452 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 44971 1.5243021989866685 No Hit GGTATCAACGCAGAGTACTTTTTTT 28575 0.9685560769394509 No Hit TATCAACGCAGAGTACTTTTTTTTT 26476 0.8974099980069608 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 15026 0.5093096627153872 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 8787 0.2978373490137167 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7781 0.2637387518693217 No Hit GTACTGGTTCACTATCGGTCAGTCA 7146 0.24221528349288945 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 5409 0.18333927629625513 No Hit ATACAGGGTGACAGCCCCGTACACA 4888 0.1656798636598438 No Hit GGATACCACGTGTCCCGCCCTACTC 4719 0.15995157050139175 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4350 0.14744423218500827 No Hit GTTAATGATAGTGTGTCGAAACACA 3934 0.13334381825651093 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 3546 0.12019247065012399 No Hit GGTATCAACGCAGAGTACATGGGGG 3135 0.10626153285057494 No Hit GTGGTATCAACGCAGAGTACTTTTT 3006 0.10188904872370917 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.389522578965707E-5 0.0 2 0.0 0.0 0.0 3.389522578965707E-5 0.0 3 0.0 0.0 0.0 3.389522578965707E-5 0.0 4 0.0 0.0 0.0 3.389522578965707E-5 0.0 5 0.0 0.0 0.0 3.389522578965707E-5 0.0 6 0.0 0.0 0.0 3.389522578965707E-5 0.0 7 0.0 0.0 0.0 6.779045157931414E-5 0.0 8 0.0 0.0 0.0 1.6947612894828537E-4 0.0 9 0.0 0.0 0.0 1.6947612894828537E-4 0.0 10 0.0 0.0 0.0 2.3726658052759952E-4 0.0 11 0.0 0.0 0.0 2.3726658052759952E-4 0.0 12 0.0 0.0 0.0 3.7284748368622783E-4 0.0 13 0.0 0.0 0.0 5.084283868448562E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCGTA 25 0.006081828 18.973682 11 GGTATCA 5590 0.0 15.666508 1 CCTAGAC 165 7.2759576E-12 11.5138445 3 CTATACT 310 0.0 11.058419 4 TGTCCCG 1035 0.0 10.632595 11 GTATCAA 12635 0.0 10.581187 1 CGCGCCT 895 0.0 10.38184 12 CTAAGAC 230 0.0 10.324914 3 GCGCCTT 920 0.0 10.305841 13 TATCGCG 900 0.0 10.157363 7 TCGCGCG 920 0.0 10.142353 9 GTGGTAT 1160 0.0 10.082576 1 CGCCTTT 1040 0.0 10.028378 14 TGGACCG 105 4.0020714E-5 9.975982 5 TCTATAC 305 0.0 9.966082 3 GTATAGA 345 0.0 9.922195 1 CCCGAAC 115 1.0511629E-5 9.893775 16 CGCGCGC 955 0.0 9.770643 10 TGTATCG 1045 0.0 9.7503395 5 ATCGCGC 970 0.0 9.718868 8 >>END_MODULE