FastQCFastQC Report
Wed 25 May 2016
SRR1294639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627187
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT219210.8343905477607799No Hit
GGTATCAACGCAGAGTACTTTTTTT137440.5231450977794881No Hit
TATCAACGCAGAGTACTTTTTTTTT127200.4841680474210629No Hit
GTACTGGTTCACTATCGGTCAGTCA106880.4068229631160629No Hit
GTACATGGGAAGCAGTGGTATCAAC89890.34215303288269927No Hit
CCCATGTACTCTGCGTTGATACCAC84250.3206852043649729No Hit
GGTCGGGAGTGGGTAATTTGCGCGC81730.3110931958783292No Hit
GGATACCACGTGTCCCGCCCTACTC77280.29415492692374007No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64150.2441775176262672No Hit
ATACAGGGTGACAGCCCCGTACACA61640.23462357266536413No Hit
GGTATCAACGCAGAGTACATGGGGG61630.23458550913962348No Hit
GTTAATGATAGTGTGTCGAAACACA59020.22465092892131394No Hit
GTACATGGGGGCGGCGACGACCCAT55780.21231834658134346No Hit
ACCCTGTATCGCGCGCCTTTCCAGA48580.18491260804807574No Hit
CCTTGGATGTGGTAGCCGTTTCTCA42950.16348284305609004No Hit
CTACCTCCCCGGGTCGGGAGTGGGT42830.16302608074720223No Hit
GTACTTTTTTTTTTTTTTTTTTTTT42200.1606280786255413No Hit
GTACATGGGGTGGCGGCGACGACCC41450.1577733141949926No Hit
GTACATGGGGGTTAAGCGACTAAGC41330.1573165518861048No Hit
GAGTACATGGGAAGCAGTGGTATCA41180.15674559899999504No Hit
GTATTTAGCCTTGGAGGATGGTCCC40990.1560223920109227No Hit
ATCATTAACTGAATCCATAGGTTAA40620.15461404155851868No Hit
AGCGTACACGGTGGATGCCCTGGCA40290.15335794520907722No Hit
GTATCAACGCAGAGTACATGGGGGC40270.15328181815759595No Hit
ACTTAGATGTTTCAGTTCCCCCGGT39940.1520257218081545No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39350.14977997378945618No Hit
GTTCACTATCGGTCAGTCAGGAGTA38930.1481813057083489No Hit
CTATCGGTCAGTCAGGAGTATTTAG38000.14464139781446847No Hit
GTGGATGCCCTGGCAGTCAGAGGCG37330.14209114158984495No Hit
CCCATATTCAGACAGGATACCACGT37170.14148212517799455No Hit
GTATCAACGCAGAGTACATGGGGGT36290.1381325349128174No Hit
GTGGTATCAACGCAGAGTACATGGG34600.13169979906264762No Hit
TATTCAGACAGGATACCACGTGTCC34340.13071014739339074No Hit
TCATTAACCTATGGATTCAGTTAAT34270.13044370271320618No Hit
GCCCAGAGCCTGAATCAGTGTGTGT33510.12755087475691682No Hit
GTCAGGAGTATTTAGCCTTGGAGGA32970.12549544436692173No Hit
GCCTTGGAGGATGGTCCCCCCATAT32220.12264067993637301No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32200.12256455288489172No Hit
CATGTACTCTGCGTTGATACCACTG31880.12134652006119091No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG31630.12039493191767468No Hit
ATTCGAACGTCTGCCCTATCAACTT30590.11643632524064712No Hit
GGTTAATGAGGCGAACCGGGGGAAC29190.11110743163695617No Hit
GTACTTAGATGTTTCAGTTCCCCCG29070.11065066932806839No Hit
GTATCAACGCAGAGTACATGGGAAG28740.10939457297862694No Hit
CTCCGGAATCGAACCCTGATTCCCC27850.10600691918770913No Hit
GCGTTGATACCACTGCTTCCCATGT26880.10231475719086613No Hit
GGTATCAACGCAGAGTACATGGGAA26720.10170574077901573No Hit
CCTATGGATTCAGTTAATGATAGTG26460.10071608910975885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG402.760421E-416.6262661
CGTCACT2700.013.72326851
AATCGCC3100.012.26367118
ATCGCCG3150.012.06096519
CCCTAAT951.0397107E-612.0009151
GTATTAA3250.011.6931991
GTGCACG907.4596046E-611.6119961
GTCCTGT1157.07314E-811.5660981
AAATCGC3350.011.06433917
GGTATCA73050.011.0158981
TGTCCCG16400.011.002954511
AGTGACG1303.279456E-810.95924219
CGACCCA14750.010.88972418
GTACTGG25650.010.8526781
TACTGGT23650.010.8026162
GACCCAT15350.010.51896819
GAAATCG3800.010.50440216
ACTGGTT24600.010.4240483
GTGTCCC17550.010.28196210
TGGTTCA24550.010.0972965
Produced by FastQC (version 0.11.5)