FastQCFastQC Report
Wed 25 May 2016
SRR1294639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627187
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT618762.355218718728435No Hit
GGTATCAACGCAGAGTACTTTTTTT421911.6059382145237473No Hit
TATCAACGCAGAGTACTTTTTTTTT368191.4014609542449776No Hit
ACGCAGAGTACTTTTTTTTTTTTTT227500.8659452105997784No Hit
GTACTTTTTTTTTTTTTTTTTTTTT125380.4772404857362647No Hit
GAGTACTTTTTTTTTTTTTTTTTTT116510.4434781384043085No Hit
GTACTGGTTCACTATCGGTCAGTCA101730.38722024735962834No Hit
CCCATGTACTCTGCGTTGATACCAC95730.3643821319152386No Hit
GTACATGGGAAGCAGTGGTATCAAC90290.3436755739123252No Hit
GGTCGGGAGTGGGTAATTTGCGCGC84800.3227786982807086No Hit
GGATACCACGTGTCCCGCCCTACTC80990.3082764949735211No Hit
GCAGAGTACTTTTTTTTTTTTTTTT77740.29590584910781No Hit
GGTATCAACGCAGAGTACATGGGGG65440.24908771244681097No Hit
ATACAGGGTGACAGCCCCGTACACA64940.2471845361597785No Hit
GTTAATGATAGTGTGTCGAAACACA60840.23157849060611216No Hit
ATCAACGCAGAGTACTTTTTTTTTT57560.2190936541631791No Hit
GTACATGGGGGCGGCGACGACCCAT53470.2035256721352534No Hit
ACCCTGTATCGCGCGCCTTTCCAGA52550.20002382776711367No Hit
GTGGTATCAACGCAGAGTACTTTTT51860.19739744449100882No Hit
CCTTGGATGTGGTAGCCGTTTCTCA50380.1917640426813927No Hit
AGCGTACACGGTGGATGCCCTGGCA47800.1819436530403051No Hit
GAGTACATGGGAAGCAGTGGTATCA44290.16858335550533707No Hit
CTATCGGTCAGTCAGGAGTATTTAG43480.16550020992034445No Hit
ACTTAGATGTTTCAGTTCCCCCGGT43320.16489119350849407No Hit
GTACATGGGGTGGCGGCGACGACCC42160.16047582452257872No Hit
CTACCTCCCCGGGTCGGGAGTGGGT42140.16039969747109742No Hit
GTATCAACGCAGAGTACATGGGGGC40690.1548804862387032No Hit
ATCATTAACTGAATCCATAGGTTAA40580.15446178745555608No Hit
CCCATATTCAGACAGGATACCACGT40000.1522541029625984No Hit
GTGGATGCCCTGGCAGTCAGAGGCG38400.14616393884409445No Hit
GTACATGGGGGTTAAGCGACTAAGC37070.14110148992058807No Hit
GTGGTATCAACGCAGAGTACATGGG36470.13881767837614908No Hit
GTCAGGAGTATTTAGCCTTGGAGGA36050.1372190102950418No Hit
GTATTTAGCCTTGGAGGATGGTCCC35990.13699062914059792No Hit
CATGTACTCTGCGTTGATACCACTG35870.13653386683171012No Hit
GTTCACTATCGGTCAGTCAGGAGTA35740.13603904099708167No Hit
GCCCAGAGCCTGAATCAGTGTGTGT35590.13546808811097194No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG35000.1332223400922736No Hit
TCATTAACCTATGGATTCAGTTAAT34960.133070085989311No Hit
GCCTTGGAGGATGGTCCCCCCATAT34910.13287976836060778No Hit
GTATCAACGCAGAGTACATGGGGGT34830.13257526015468254No Hit
ATTCGAACGTCTGCCCTATCAACTT34240.13032951213598423No Hit
TATTCAGACAGGATACCACGTGTCC33110.12602833372729083No Hit
CTCCGGAATCGAACCCTGATTCCCC31760.12088975775230314No Hit
GGTTAATGAGGCGAACCGGGGGAAC30490.11605568998324063No Hit
GCGTTGATACCACTGCTTCCCATGT30320.11540861004564959No Hit
GTATCAACGCAGAGTACATGGGAAG30100.1145712124793553No Hit
GTACTTAGATGTTTCAGTTCCCCCG29440.11205901978047243No Hit
GGTATCAACGCAGAGTACATGGGAA29150.11095517753399357No Hit
CCTATGGATTCAGTTAATGATAGTG28970.11027003407066188No Hit
CAACGCAGAGTACTTTTTTTTTTTT27160.10338053591160432No Hit
GCCTGAATCAGTGTGTGTGTTAGTG26710.1016676772532751No Hit
CACACACACTGATTCAGGCTCTGGG26700.10162961372753444No Hit
GAGTACATGGGGGCGGCGACGACCC26470.1007541526354995No Hit
ACGCAGAGTACATGGGAAGCAGTGG26340.10025932680087105No Hit
GGTATCAACGCAGAGTACATGGGGT26300.10010707269790846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG250.00595988719.0402375
CGTCACT3050.016.51981
GACCCGA852.6327143E-713.4401686
CGCCTAA1205.2750693E-1113.43498214
CGACCGC1301.4551915E-1113.18019210
TCGACCG1403.6379788E-1212.9186799
CCGCCTA1251.05501385E-1012.89758513
AGACCCG1051.9375875E-812.6934915
GGTATCA106900.012.3168831
ACATCGA1551.8189894E-1212.2840246
ATCGACC1551.8189894E-1212.2840248
AAATCGC3750.012.13867217
ATCGCCG3900.011.91541619
GAAATCG3850.011.82338216
ACCGCCT1552.5465852E-1111.62495712
CGAACAC1251.8819264E-811.38000518
AATCGCC4000.011.38000418
TGTCCCG16050.011.35038511
GTGTCCC16900.010.92716810
TCCCGCC17100.010.75907913
Produced by FastQC (version 0.11.5)