FastQCFastQC Report
Wed 25 May 2016
SRR1294638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589567
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79630.3075031462788953No Hit
GTATCAACGCAGAGTACTTTTTTTT72400.2795834207031523No Hit
CCCATGTACTCTGCGTTGATACCAC68520.26460022080911594No Hit
GGTATCAACGCAGAGTACTTTTTTT51890.2003809903354499No Hit
TATCAACGCAGAGTACTTTTTTTTT40610.1568215844579422No Hit
GAGTACATGGGAAGCAGTGGTATCA37450.14461877217310848No Hit
ATTCCATTCCATTCCATTCCATTCC32170.12422926303895594No Hit
CATGTACTCTGCGTTGATACCACTG26740.10326050648621951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG508.737123E-515.1959955
CCCGTAC853.9271345E-612.299437516
CCCCGTA951.0354688E-612.00495915
TATTCCG658.044349E-411.6892275
GTCTAGG2100.010.858061
CGAGTAC700.001495266110.8542823
TAGGACC1252.249435E-710.6374024
CGCCCTA1002.3886334E-510.45452116
CCGTACA1106.01793E-610.36812117
TCTACAC3000.010.1306643
CTAGGAC1901.0568328E-99.4974973
CGTTCCG1304.2571373E-69.49712910
AATCCGA800.00453204759.49621211
TGTCCCG1201.7092745E-59.49621111
AACAGTC2451.8189894E-129.3034917
GTATTAG3900.09.2571921
GTCTAAG2501.8189894E-129.120771
AGCGTAC950.001822919.000761
CTAAGAC1804.2391548E-88.9698583
GTCTAAT2451.6370905E-118.9191211
Produced by FastQC (version 0.11.5)