##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294638_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2589567 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.911673264294766 31.0 30.0 33.0 25.0 34.0 2 30.12486913835402 31.0 30.0 34.0 25.0 34.0 3 30.063383955696068 31.0 30.0 34.0 25.0 34.0 4 33.70466375266599 35.0 35.0 37.0 28.0 37.0 5 34.021263400406326 35.0 35.0 37.0 28.0 37.0 6 34.1074334048897 37.0 35.0 37.0 29.0 37.0 7 33.99344446388141 37.0 35.0 37.0 29.0 37.0 8 33.86244881866351 37.0 35.0 37.0 28.0 37.0 9 34.98688970009272 39.0 35.0 39.0 27.0 39.0 10 35.096428476266496 38.0 35.0 39.0 27.0 39.0 11 35.32210906302096 39.0 35.0 39.0 28.0 39.0 12 35.111577340922246 38.0 35.0 39.0 27.0 39.0 13 34.971810731292145 38.0 35.0 39.0 27.0 39.0 14 35.85418527498999 39.0 35.0 41.0 27.0 41.0 15 35.84810819723915 39.0 34.0 41.0 27.0 41.0 16 35.963498530835466 39.0 35.0 41.0 27.0 41.0 17 35.94872656316674 39.0 35.0 41.0 27.0 41.0 18 35.8418747226853 39.0 35.0 41.0 27.0 41.0 19 36.13607101109954 39.0 35.0 41.0 27.0 41.0 20 36.24917563438212 39.0 35.0 41.0 27.0 41.0 21 36.30250810270597 39.0 36.0 41.0 27.0 41.0 22 36.29782855589371 39.0 35.0 41.0 27.0 41.0 23 36.280625679891656 39.0 35.0 41.0 27.0 41.0 24 36.28737391231816 39.0 35.0 41.0 27.0 41.0 25 35.031200196789655 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8839490429581041 1101 2 0.9394011295620466 1101 3 1.219347602838397 1101 4 1.0788943445139836 1101 5 0.731488996228876 1101 6 0.7955017459556686 1101 7 0.6999090026689103 1101 8 0.9394592554369297 1101 9 1.155419568449659 1101 10 1.1230041800989028 1101 11 0.9258395692125276 1101 12 1.0810907242637953 1101 13 1.1521707648349775 1101 14 1.257243557249069 1101 15 1.386742748532015 1101 16 1.1766647547429017 1101 17 1.206960128603022 1101 18 1.2028002942835698 1101 19 1.0953377435793428 1101 20 1.1451322813121152 1101 21 0.9750850188389677 1101 22 0.8873234328145898 1101 23 0.8086461471782513 1101 24 0.9480229793548176 1101 25 0.904629756212735 1102 1 0.939581963262242 1102 2 0.9262372320683845 1102 3 1.1708194886921461 1102 4 1.0419939408718193 1102 5 0.8508996082630418 1102 6 0.7059268609467466 1102 7 0.7075733929814945 1102 8 0.7990607761353274 1102 9 1.1355893563028445 1102 10 1.0254548982064762 1102 11 0.8042679461955373 1102 12 1.0724873965626784 1102 13 0.9926768429350474 1102 14 1.259752357586997 1102 15 1.4264565615424374 1102 16 1.219060324066092 1102 17 1.267682108327044 1102 18 1.3973424420353737 1102 19 1.034229253013308 1102 20 0.31632431641959613 1102 21 0.6672592889976059 1102 22 0.7408680915961554 1102 23 0.9081649447000757 1102 24 1.012022322262922 1102 25 1.0074340132295347 1103 1 0.8322920875690087 1103 2 0.8163476088674386 1103 3 1.0119411089193768 1103 4 0.9046442272410857 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-0.27650524156656786 2207 11 0.11585544565665629 2207 12 0.4730181018465629 2207 13 0.6432198226684349 2207 14 0.9188497304955519 2207 15 0.8557840234976268 2207 16 0.8086805705609876 2207 17 0.8210162380027128 2207 18 0.7051985088466992 2207 19 -0.3236163056717345 2207 20 0.215471900807934 2207 21 -0.3558798445819349 2207 22 0.2742194174762034 2207 23 -0.5384874536674857 2207 24 -1.6387959344538956 2207 25 -0.2669497794675877 2208 1 0.4833459760699057 2208 2 0.1378828714066067 2208 3 0.6524388763376159 2208 4 0.46313850759196384 2208 5 0.3469504537245811 2208 6 0.30462532274041365 2208 7 0.28955241317410696 2208 8 0.46519984103904477 2208 9 -0.2528558182735665 2208 10 0.038994044929062 2208 11 0.18572247120341956 2208 12 0.18075470957471396 2208 13 0.5022664661668799 2208 14 0.7644082033259849 2208 15 0.7081163199465763 2208 16 0.5280463487439846 2208 17 0.43324055302493747 2208 18 0.7510966200510154 2208 19 -1.379854046671582 2208 20 -0.2114561301130209 2208 21 -0.251525237556919 2208 22 0.1751152975943171 2208 23 0.33286447734084135 2208 24 0.607585538251719 2208 25 0.6510326394873687 2209 1 0.07773904491339323 2209 2 -1.415213721000029 2209 3 -0.3015159737158939 2209 4 0.12173274412405988 2209 5 0.09525985146475335 2209 6 0.17073357995067795 2209 7 0.1518126890972198 2209 8 0.29883046470292385 2209 9 -2.0600968120552636 2209 10 -0.439874723276759 2209 11 -0.062113734328626435 2209 12 0.35895365944548985 2209 13 0.4353649325950002 2209 14 0.5661362307715265 2209 15 0.7287809531799354 2209 16 0.6799948481528162 2209 17 0.4056116025792633 2209 18 0.7237859111017002 2209 19 0.3778616344723247 2209 20 0.29843200634368827 2209 21 0.248748862143799 2209 22 0.27517147190526003 2209 23 0.2836766525212937 2209 24 0.48121447394188266 2209 25 0.3895944523825392 2210 1 -0.07023214598907046 2210 2 0.0032741882213045415 2210 3 -0.023882849072698775 2210 4 0.12904496885544603 2210 5 0.05804846976229783 2210 6 0.1274019921895828 2210 7 -0.004330351545291933 2210 8 0.09740840389780203 2210 9 0.29024146051982314 2210 10 -1.5471936351104887 2210 11 -0.6379345961192229 2210 12 -2.353977173286907 2210 13 -0.8943186933647453 2210 14 -0.6053062534830005 2210 15 -0.8102050169451687 2210 16 -0.27620314223671727 2210 17 -0.03539081040768366 2210 18 0.24409913773610015 2210 19 0.16937383821616692 2210 20 0.11676937040272861 2210 21 0.054368418180501976 2210 22 -0.009823204383955897 2210 23 -0.0013209296244554025 2210 24 -0.017054598129867315 2210 25 -0.239533947937268 2211 1 -0.26221451312716937 2211 2 -0.10415110322441379 2211 3 -1.4718627699799072 2211 4 -0.549712771147874 2211 5 -0.3633971297052767 2211 6 -0.38310901150927634 2211 7 -0.4604336277964265 2211 8 -0.08342703904348525 2211 9 0.17377690457604444 2211 10 0.14676354419794535 2211 11 -0.181428205645787 2211 12 -0.12541191077637137 2211 13 -0.23895924753511366 2211 14 -0.5676810634466065 2211 15 -0.24477287359930955 2211 16 0.023277546801963922 2211 17 -0.06140211342338375 2211 18 0.08242973329540604 2211 19 -0.015684639965726888 2211 20 0.0067926425428623816 2211 21 -0.10922747796316656 2211 22 -0.11618509833486002 2211 23 -0.49427337499310653 2211 24 -0.2762333554262071 2211 25 -0.07608349543586002 2212 1 -0.25558998280889966 2212 2 -0.21852565317333372 2212 3 -2.2622355231542883 2212 4 -1.2153155693647548 2212 5 -0.5111265758158225 2212 6 -0.3655959559005737 2212 7 -0.20252528690161853 2212 8 -0.308336668808316 2212 9 -0.9431599194899007 2212 10 -0.0321020550469413 2212 11 -0.20960768217053527 2212 12 0.08394112470191573 2212 13 -0.34615245111569237 2212 14 0.0619034607739124 2212 15 0.1733016506199263 2212 16 -0.9784197004373567 2212 17 -1.2470604509416745 2212 18 -0.07619484201465809 2212 19 0.08088883872689223 2212 20 -0.04582121236465042 2212 21 0.08683150508510806 2212 22 -0.1702556903736081 2212 23 0.07801226818950369 2212 24 0.020430601081386612 2212 25 0.2711784620812381 2213 1 -3.064750317383048 2213 2 -1.4449911945317702 2213 3 -2.3124079053350215 2213 4 -1.0042613058058478 2213 5 -0.7223134202601074 2213 6 -0.5489474367024698 2213 7 -0.4477669035144629 2213 8 -0.1720261745559668 2213 9 -0.2973736085226264 2213 10 -0.21517634584282064 2213 11 -0.5163494855138424 2213 12 -0.20396968796079307 2213 13 -0.48669206387859276 2213 14 -0.13807982583551137 2213 15 -0.08528283782192858 2213 16 -1.6621696446885181 2213 17 -0.9714620462370576 2213 18 -0.4095095848919783 2213 19 -0.14324079231262488 2213 20 -0.18204611243344004 2213 21 0.01593789730378603 2213 22 0.02713864248055131 2213 23 0.024722906240086218 2213 24 0.0024934839745753834 2213 25 0.03954091470598087 2214 1 -1.0365716331853925 2214 2 -0.7464696017431933 2214 3 -6.424066507578086 2214 4 -2.2792042872211375 2214 5 -1.3775719014915282 2214 6 -0.8681643754339845 2214 7 -0.7322795870925631 2214 8 -0.5360766588742649 2214 9 -0.15605610331894582 2214 10 -0.3880447384350205 2214 11 -0.8776614473698103 2214 12 -0.5221402745230677 2214 13 -5.142976607902014 2214 14 -4.274372449378799 2214 15 -2.2608926513589296 2214 16 -2.9937902768113105 2214 17 -3.2819248433088717 2214 18 -1.0812290914978036 2214 19 -0.8464747741210275 2214 20 -0.5204283606775917 2214 21 -0.5390318226122304 2214 22 -0.3885655166606341 2214 23 -0.4000714373713876 2214 24 -0.238846665623349 2214 25 -0.15877995817201196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1007.0 3 132.0 4 110.0 5 275.0 6 40.0 7 47.0 8 73.0 9 267.0 10 670.0 11 1615.0 12 3368.0 13 5838.0 14 9198.0 15 12590.0 16 15615.0 17 17735.0 18 19094.0 19 19629.0 20 19804.0 21 20000.0 22 20433.0 23 20927.0 24 22230.0 25 24009.0 26 26297.0 27 30233.0 28 34787.0 29 42322.0 30 52528.0 31 65644.0 32 85673.0 33 113990.0 34 152859.0 35 207880.0 36 290533.0 37 433274.0 38 818841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34782761634436 21.340341716669947 13.001947818261868 22.30988284872383 2 14.842109736920397 21.87285322947697 41.33448471711891 21.95055231648372 3 18.312412001353884 28.157287099301886 29.67553904242018 23.854761856924053 4 11.573221029856368 17.488201373528188 39.45325464362642 31.48532295298902 5 12.308953599470852 39.31142741347543 35.08442378060442 13.295195206449304 6 29.621739387491935 39.33273540382847 18.05381288258568 12.991712326093904 7 24.85060424857753 34.12567984002627 23.822560545610866 17.201155365785333 8 23.567940475721276 37.730265928795596 21.773919951653525 16.927873643829603 9 24.46668413279513 17.16244195024008 22.50089455945555 35.86997935750924 10 14.285570757799238 31.046092720247586 34.00152018143253 20.66681634052064 11 31.12488997388739 27.157149359626775 23.08512060562082 18.632840060865018 12 19.914575093392436 29.23909700294126 32.2202361356474 18.626091768018906 13 25.829681836799356 25.206531735175698 27.206006228796202 21.757780199228744 14 19.649433020006015 24.31232471457795 29.154008505665935 26.8842337597501 15 22.65552546648366 31.1404891741168 24.864599176466225 21.339386182933314 16 20.636378530348672 32.37503419644913 26.477827563342643 20.510759709859553 17 18.77701010850413 31.20660607746762 29.32652477665461 20.689859037373644 18 19.075059564484135 30.712650719124408 30.466116983448018 19.746172732943442 19 19.837151625740105 29.494405685743057 30.881217525579586 19.787225162937254 20 21.766153774817035 28.917826533845453 29.675803921265565 19.64021577007195 21 22.092572607197603 29.645738158524672 28.629625722710305 19.63206351156742 22 20.289927875465875 29.25686419605199 30.34512739167312 20.108080536809016 23 20.171321898220047 29.776260618259677 29.7421013440747 20.31031613944558 24 20.10542465499318 28.979104431468176 30.087686415754956 20.827784497783686 25 20.820011051986846 29.025531635346262 29.035888042600384 21.118569270066505 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2556.0 1 2556.0 2 2672.0 3 2788.0 4 2788.0 5 2788.0 6 4627.0 7 6466.0 8 6466.0 9 6466.0 10 10084.0 11 13702.0 12 13702.0 13 13702.0 14 22147.5 15 30593.0 16 30593.0 17 30593.0 18 47130.0 19 63667.0 20 63667.0 21 63667.0 22 88560.0 23 113453.0 24 113453.0 25 113453.0 26 144237.0 27 175021.0 28 175021.0 29 175021.0 30 206821.5 31 238622.0 32 238622.0 33 238622.0 34 263123.0 35 287624.0 36 287624.0 37 287624.0 38 300082.0 39 312540.0 40 312540.0 41 312540.0 42 306787.0 43 301034.0 44 301034.0 45 301034.0 46 303129.5 47 305225.0 48 305225.0 49 305225.0 50 281144.5 51 257064.0 52 257064.0 53 257064.0 54 226068.0 55 195072.0 56 195072.0 57 195072.0 58 164997.5 59 134923.0 60 134923.0 61 134923.0 62 109018.0 63 83113.0 64 83113.0 65 83113.0 66 62070.0 67 41027.0 68 41027.0 69 41027.0 70 28691.0 71 16355.0 72 16355.0 73 16355.0 74 11195.0 75 6035.0 76 6035.0 77 6035.0 78 3973.5 79 1912.0 80 1912.0 81 1912.0 82 1241.5 83 571.0 84 571.0 85 571.0 86 351.0 87 131.0 88 131.0 89 131.0 90 88.5 91 46.0 92 46.0 93 46.0 94 28.0 95 10.0 96 10.0 97 10.0 98 13.5 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09924439105070462 2 0.0531749130260001 3 0.05634146558092531 4 0.051823335715971044 5 0.048502317182756806 6 0.06750163251230805 7 0.06340828408764863 8 0.061052677918740854 9 0.06568665726741188 10 0.06634313767514029 11 0.05966248411413955 12 0.060357581016440204 13 0.061284376886174406 14 0.06352413357136541 15 0.06545495829997834 16 0.06097544492959634 17 0.06360136656050992 18 0.14342166084136845 19 0.0680036469417474 20 0.06282903666906475 21 0.06047343050015698 22 0.06495294387053897 23 0.06526187582711704 24 0.07480014998646492 25 0.06939384074634872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2589567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.62168711490074 #Duplication Level Percentage of deduplicated Percentage of total 1 92.3428915483751 76.29525492795058 2 5.966502054563938 9.85924931845188 3 0.8340810281873853 2.0673954521811857 4 0.26663774304480964 0.8812024071548623 5 0.1448527072756938 0.5983987529139339 6 0.08575081011400945 0.42509259618533723 7 0.06372557095320312 0.3685579929159768 8 0.04714519080062822 0.31161721626414407 9 0.03228872742624303 0.2400974221274429 >10 0.18622420298791997 2.8761213161327905 >50 0.015836199322248397 0.9086813951131333 >100 0.011521974482643558 1.7803934440797304 >500 0.0015535926182448385 0.8797358012196943 >1k 8.003355911878431E-4 1.44848925590071 >5k 1.8831425675008072E-4 1.05971270140871 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 7963 0.3075031462788953 No Hit GTATCAACGCAGAGTACTTTTTTTT 7240 0.2795834207031523 No Hit CCCATGTACTCTGCGTTGATACCAC 6852 0.26460022080911594 No Hit GGTATCAACGCAGAGTACTTTTTTT 5189 0.2003809903354499 No Hit TATCAACGCAGAGTACTTTTTTTTT 4061 0.1568215844579422 No Hit GAGTACATGGGAAGCAGTGGTATCA 3745 0.14461877217310848 No Hit ATTCCATTCCATTCCATTCCATTCC 3217 0.12422926303895594 No Hit CATGTACTCTGCGTTGATACCACTG 2674 0.10326050648621951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.8616494572258606E-5 0.0 2 0.0 0.0 0.0 3.8616494572258606E-5 0.0 3 0.0 0.0 0.0 3.8616494572258606E-5 0.0 4 0.0 0.0 0.0 7.723298914451721E-5 0.0 5 0.0 0.0 0.0 7.723298914451721E-5 0.0 6 0.0 0.0 0.0 7.723298914451721E-5 0.0 7 0.0 0.0 0.0 7.723298914451721E-5 0.0 8 0.0 0.0 0.0 7.723298914451721E-5 0.0 9 0.0 0.0 0.0 1.1584948371677582E-4 0.0 10 0.0 0.0 0.0 1.5446597828903442E-4 0.0 11 0.0 0.0 0.0 1.9308247286129303E-4 0.0 12 0.0 0.0 0.0 1.9308247286129303E-4 0.0 13 0.0 0.0 0.0 1.9308247286129303E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 50 8.737123E-5 15.195995 5 CCCGTAC 85 3.9271345E-6 12.2994375 16 CCCCGTA 95 1.0354688E-6 12.004959 15 TATTCCG 65 8.044349E-4 11.689227 5 GTCTAGG 210 0.0 10.85806 1 CGAGTAC 70 0.0014952661 10.854282 3 TAGGACC 125 2.249435E-7 10.637402 4 CGCCCTA 100 2.3886334E-5 10.454521 16 CCGTACA 110 6.01793E-6 10.368121 17 TCTACAC 300 0.0 10.130664 3 CTAGGAC 190 1.0568328E-9 9.497497 3 CGTTCCG 130 4.2571373E-6 9.497129 10 AATCCGA 80 0.0045320475 9.496212 11 TGTCCCG 120 1.7092745E-5 9.496211 11 AACAGTC 245 1.8189894E-12 9.303491 7 GTATTAG 390 0.0 9.257192 1 GTCTAAG 250 1.8189894E-12 9.12077 1 AGCGTAC 95 0.00182291 9.00076 1 CTAAGAC 180 4.2391548E-8 8.969858 3 GTCTAAT 245 1.6370905E-11 8.919121 1 >>END_MODULE