FastQCFastQC Report
Wed 25 May 2016
SRR1294638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589567
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT182580.7050599579002976No Hit
GGTATCAACGCAGAGTACTTTTTTT148480.5733777114088958No Hit
TATCAACGCAGAGTACTTTTTTTTT111020.4287203227412151No Hit
GTACATGGGAAGCAGTGGTATCAAC79340.3063832679362998No Hit
CCCATGTACTCTGCGTTGATACCAC77470.2991619834512874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT73150.2824796577960717No Hit
GAGTACATGGGAAGCAGTGGTATCA40510.15643541951221962No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32080.12388171458780561No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31880.12310938469636044No Hit
ATTCCATTCCATTCCATTCCATTCC31540.12179642388090363No Hit
CATGTACTCTGCGTTGATACCACTG30820.11901603627170103No Hit
GAATGGAATGGAATGGAATGGAATG26900.10387837039937564No Hit
GGTATCAACGCAGAGTACATGGGAA26060.10063458485530592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA550.003022776212.11563910
CGCAGCG700.00146605310.8799838
CGCGGTG700.001513902810.83817614
TTCCGTA1303.73484E-710.2522927
ACTATCG1651.0417352E-89.7770111
GTCGCCC2054.1836756E-119.720911
TATTCCG1304.1288113E-69.5199855
TGAACCG800.00443952679.5199835
CCGTACC1106.688202E-59.5194319
CGGTCAG1701.7131242E-89.4837716
CGCCCTA1106.968529E-59.4837716
TCTAGTA1651.06960215E-79.2102562
GGTATCA59450.09.1156411
TAGACAG4100.09.0555945
GTCTAGT2002.6211637E-99.032081
CTAGGAC2651.8189894E-128.9604493
TCCGTAT850.00731042968.9599868
CAGCGTA850.0073139188.95946510
CTATCGG1804.3424734E-88.95654812
CGCGCCT850.00754496978.92555612
Produced by FastQC (version 0.11.5)