##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294638_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2589567 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36379479658182 31.0 31.0 34.0 28.0 34.0 2 31.485225908424074 31.0 31.0 34.0 28.0 34.0 3 31.572960653267515 31.0 31.0 34.0 28.0 34.0 4 35.159549067469584 37.0 35.0 37.0 32.0 37.0 5 35.293014237515386 37.0 35.0 37.0 32.0 37.0 6 34.919161388757274 37.0 35.0 37.0 32.0 37.0 7 34.77148689336866 37.0 35.0 37.0 32.0 37.0 8 34.76321099241688 37.0 35.0 37.0 32.0 37.0 9 36.220946976849795 39.0 35.0 39.0 31.0 39.0 10 35.995938703265836 39.0 35.0 39.0 30.0 39.0 11 36.16656105055401 39.0 35.0 39.0 31.0 39.0 12 36.10719591344808 39.0 35.0 39.0 30.0 39.0 13 35.97537155825665 39.0 35.0 39.0 30.0 39.0 14 37.02628547552545 40.0 36.0 41.0 30.0 41.0 15 37.08727366389825 40.0 36.0 41.0 31.0 41.0 16 37.12775765214802 40.0 36.0 41.0 31.0 41.0 17 37.17983508439828 40.0 36.0 41.0 31.0 41.0 18 37.19703911889517 40.0 37.0 41.0 31.0 41.0 19 37.243994459305355 40.0 37.0 41.0 31.0 41.0 20 37.308047638852365 40.0 37.0 41.0 31.0 41.0 21 37.192234454640484 40.0 37.0 41.0 30.0 41.0 22 37.153298987823064 40.0 37.0 41.0 30.0 41.0 23 37.177635102702496 40.0 37.0 41.0 30.0 41.0 24 37.111213573543374 40.0 36.0 41.0 30.0 41.0 25 36.0348440492175 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48285503958006615 1101 2 0.45437323493973025 1101 3 0.40412286499011785 1101 4 0.334370676693986 1101 5 0.3947882974564436 1101 6 0.45618857956156944 1101 7 0.49783092081385405 1101 8 0.5711420075925986 1101 9 0.7237315004116951 1101 10 0.76428164807038 1101 11 0.792577134368571 1101 12 0.8296062804296369 1101 13 0.7587072593114002 1101 14 0.9968037029772177 1101 15 0.8986619518849466 1101 16 1.052059641395168 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0.17359737469023173 2207 6 0.32456857599979116 2207 7 0.3138486993999834 2207 8 0.26609788320312333 2207 9 0.34494235398844353 2207 10 0.4946109232481817 2207 11 0.43245341935642756 2207 12 0.3457096175142027 2207 13 0.4507532744754883 2207 14 0.41032876616091585 2207 15 0.5323882556289874 2207 16 0.6165224757383001 2207 17 0.6141147963560982 2207 18 0.6026672264335602 2207 19 0.6672998526766563 2207 20 0.46695634038825773 2207 21 0.8655535150218014 2207 22 0.7736742863174015 2207 23 0.6531375442227585 2207 24 0.8127802793386962 2207 25 0.8321332427385926 2208 1 0.18921271992248734 2208 2 0.20850880824646367 2208 3 0.20864435366090106 2208 4 0.1871695694089368 2208 5 0.10178771000511233 2208 6 0.2393462658812382 2208 7 0.3021244522182158 2208 8 0.22249530306380905 2208 9 0.36563226503236734 2208 10 0.4359659896439041 2208 11 0.14282951429361646 2208 12 -0.003002292997578593 2208 13 0.12138211480677796 2208 14 -0.3577240524637162 2208 15 0.22006081452687454 2208 16 0.34644123446230424 2208 17 0.30929162548578404 2208 18 0.3427072244262277 2208 19 0.6636383621395083 2208 20 0.4603628766058421 2208 21 0.7784143008187954 2208 22 0.768202370481319 2208 23 0.6769286007482336 2208 24 0.7047309406652431 2208 25 0.6801405846635262 2209 1 0.008824999916978271 2209 2 0.003349182995162181 2209 3 0.12209695882699023 2209 4 -0.5441758350986703 2209 5 -0.03291601371977748 2209 6 0.11735463083170572 2209 7 0.12757738499947635 2209 8 0.11890703663383562 2209 9 0.23710924808872846 2209 10 0.25506653132450907 2209 11 0.3459591515656726 2209 12 0.22778334649478893 2209 13 0.4541874811375308 2209 14 0.30687642650595137 2209 15 -0.21398608569438693 2209 16 -0.14644402406337775 2209 17 0.24214619824612527 2209 18 -0.15039170391361978 2209 19 0.23014379571476695 2209 20 0.2806596484737369 2209 21 0.5320737127032373 2209 22 0.5151153762866869 2209 23 0.5012763313164115 2209 24 0.5697357064465933 2209 25 0.5372912116178057 2210 1 -0.24025674033802247 2210 2 -0.23546024544864963 2210 3 -0.12476714069220307 2210 4 -0.09839775587056465 2210 5 -0.0919131103695463 2210 6 -0.6374249673965835 2210 7 -0.23793657802255552 2210 8 -0.3441137777622387 2210 9 -0.06652471801787385 2210 10 0.23444904334957783 2210 11 0.12678583069942562 2210 12 0.05514067084602203 2210 13 0.03672101681049611 2210 14 0.034358953318204044 2210 15 0.0334894786856097 2210 16 0.14848617526494223 2210 17 -0.9618183054086984 2210 18 -1.7749995721957958 2210 19 -0.4764087908989225 2210 20 -0.2753353896309392 2210 21 -0.46623714921776127 2210 22 -0.39052768449703734 2210 23 -0.24353581577412342 2210 24 0.16910824484558162 2210 25 0.08356267259420491 2211 1 -0.18633873745129392 2211 2 -0.14560786380129187 2211 3 -0.07690719342921781 2211 4 -0.06475830032051988 2211 5 -0.0627424396625571 2211 6 -0.015150670949338974 2211 7 -0.21643742288808454 2211 8 -0.06986397278981826 2211 9 -0.009075366644431426 2211 10 0.04482039414641292 2211 11 -0.28032959634567334 2211 12 -0.186756912679229 2211 13 -0.06659261758963453 2211 14 -0.1565347469389664 2211 15 -0.2323220873863292 2211 16 -0.15662325988789405 2211 17 -0.2574389896241982 2211 18 -0.40037255122841486 2211 19 -0.2733766420285164 2211 20 -0.2409037098915121 2211 21 0.0093240172988871 2211 22 -0.009331356142702418 2211 23 -0.6328081241958188 2211 24 -0.14222517513666588 2211 25 -0.7170586302390163 2212 1 -0.17888594911684663 2212 2 -0.31035869383810066 2212 3 -0.1247523868932916 2212 4 -0.07565798834759363 2212 5 -0.010212433097422036 2212 6 -0.12303413214926451 2212 7 -0.13264421249908764 2212 8 -0.33060718012610835 2212 9 -0.10629129917582958 2212 10 -0.43500268244270046 2212 11 -0.14110253405457485 2212 12 -0.25672618149484805 2212 13 -0.21319002586633928 2212 14 -0.19719961657545326 2212 15 -0.03665537653738227 2212 16 -0.26117843612342995 2212 17 -0.583415781272187 2212 18 -0.356110724042658 2212 19 -0.31357212284997615 2212 20 -0.5472473780869223 2212 21 -0.02070067663442643 2212 22 -0.33359164270037667 2212 23 -0.18300991196348093 2212 24 -0.45711080800013804 2212 25 -0.08540721029417853 2213 1 -0.16885782467011268 2213 2 -0.18484495980085214 2213 3 -0.08551466166233368 2213 4 0.015607350467973902 2213 5 -0.06408874589821068 2213 6 -0.27026486748889056 2213 7 -0.33351236276823215 2213 8 -0.27622117648699884 2213 9 -0.3885073055584556 2213 10 -0.3541547414107882 2213 11 -0.05839656854543307 2213 12 0.021447076449533142 2213 13 0.1653597113369898 2213 14 -0.47955734890622637 2213 15 -0.21026484271349233 2213 16 -0.34188492150462224 2213 17 -0.16561318218228394 2213 18 -0.12490776987203844 2213 19 -0.3183357545120771 2213 20 -0.3468849092443307 2213 21 -0.06706591962955599 2213 22 -0.39578789034146666 2213 23 -0.4552577548774295 2213 24 -0.7630255462824067 2213 25 -0.44555924358400745 2214 1 -0.2540583543677819 2214 2 -0.09649468544137463 2214 3 -0.08684252894082078 2214 4 0.05130977051967278 2214 5 0.008602658108578964 2214 6 -0.005474726676993669 2214 7 -0.08938677152310248 2214 8 -0.10513861140248082 2214 9 -0.13624440595283005 2214 10 0.07941819980617737 2214 11 0.07298765610145352 2214 12 -0.07190561633125014 2214 13 -0.16980540252808396 2214 14 -0.040101575219736674 2214 15 0.16768404617137378 2214 16 -0.19562369214951758 2214 17 -0.3256291600668604 2214 18 -0.14286386873202872 2214 19 -0.4299032357127004 2214 20 -0.3310863682679397 2214 21 -0.4385906844448755 2214 22 -0.663660748408212 2214 23 -1.0347124054019616 2214 24 -0.42742113116899816 2214 25 -0.8737922743278546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 10.0 9 31.0 10 76.0 11 173.0 12 434.0 13 975.0 14 2151.0 15 3662.0 16 5568.0 17 7228.0 18 8879.0 19 9996.0 20 10764.0 21 11651.0 22 12682.0 23 13918.0 24 15367.0 25 17556.0 26 20064.0 27 22843.0 28 26867.0 29 34003.0 30 43089.0 31 57027.0 32 76853.0 33 104518.0 34 144586.0 35 201938.0 36 291649.0 37 463804.0 38 981199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47613908520662 21.52515040145709 12.071659278660542 23.92705123467575 2 14.859009247491956 22.11049183125982 40.06399525480514 22.966503666443078 3 18.12615013520312 27.86340405605487 29.976772054078154 24.03367375466386 4 11.519538646860697 17.7151479596625 39.20397118396696 31.561342209509846 5 12.536283243948931 38.684784309029915 35.158294402675935 13.62063804434522 6 29.89761835608481 39.35208501986763 17.50162988734466 13.2486667367029 7 24.90519102781501 34.4158693494123 23.446535001487124 17.23240462128557 8 24.072884555292433 37.52212751707145 21.226850358694634 17.17813756894148 9 24.586107292358548 16.196342235746613 22.720423546857383 36.49712692503746 10 14.415372448069721 30.770715897372273 33.841212365570556 20.97269928898745 11 31.588479668855978 26.37710370315127 22.83655676061884 19.19785986737391 12 20.221438380181066 28.933039431185037 32.31064859796627 18.534873590667626 13 26.61044360329105 24.363001367796766 27.31141048345553 21.715144545456653 14 19.96787086559441 23.718870073951074 29.493425498638757 26.81983356181576 15 23.041344922557435 30.375218379228368 25.272443051485748 21.310993646728445 16 20.63602212573236 31.885058643076597 26.902212109837798 20.576707121353248 17 18.637756288119324 30.873822099919444 29.734557837104752 20.75386377485648 18 18.87016283467571 29.983715373930465 31.22570290925984 19.920418882133987 19 19.60417833214265 28.841227240254096 32.02035102624008 19.53424340136317 20 21.508154660904044 28.100466410922003 30.935849383023072 19.455529545150878 21 21.843873802450535 28.865880252197385 29.726953508300063 19.563292437052024 22 20.010210532372678 28.640620540856997 31.429941806916673 19.919227119853655 23 19.84843014174195 29.12543226371997 30.99555330721569 20.03058428732239 24 19.762377262299065 28.369414655036923 31.321413966118662 20.546794116545353 25 20.503877166424665 28.376803423028207 30.14191819333313 20.977401217214002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1380.0 1 1380.0 2 1742.0 3 2104.0 4 2104.0 5 2104.0 6 4455.5 7 6807.0 8 6807.0 9 6807.0 10 9975.0 11 13143.0 12 13143.0 13 13143.0 14 19664.0 15 26185.0 16 26185.0 17 26185.0 18 42116.5 19 58048.0 20 58048.0 21 58048.0 22 84210.5 23 110373.0 24 110373.0 25 110373.0 26 142903.0 27 175433.0 28 175433.0 29 175433.0 30 208636.5 31 241840.0 32 241840.0 33 241840.0 34 266059.0 35 290278.0 36 290278.0 37 290278.0 38 300991.0 39 311704.0 40 311704.0 41 311704.0 42 305766.0 43 299828.0 44 299828.0 45 299828.0 46 303219.5 47 306611.0 48 306611.0 49 306611.0 50 283615.5 51 260620.0 52 260620.0 53 260620.0 54 228966.0 55 197312.0 56 197312.0 57 197312.0 58 167332.5 59 137353.0 60 137353.0 61 137353.0 62 111034.0 63 84715.0 64 84715.0 65 84715.0 66 62926.0 67 41137.0 68 41137.0 69 41137.0 70 28823.5 71 16510.0 72 16510.0 73 16510.0 74 11175.0 75 5840.0 76 5840.0 77 5840.0 78 3796.0 79 1752.0 80 1752.0 81 1752.0 82 1104.0 83 456.0 84 456.0 85 456.0 86 281.5 87 107.0 88 107.0 89 107.0 90 66.5 91 26.0 92 26.0 93 26.0 94 15.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09619368797949619 2 0.0 3 0.13353583823087026 4 0.012125579295689201 5 0.021702469949609338 6 0.01664370916064346 7 0.026143366825419075 8 0.02301543076506613 9 2.7031546200581027E-4 10 0.3918415704247081 11 0.06526187582711704 12 0.0016605092666071202 13 3.8616494572258606E-5 14 6.564804077283963E-4 15 0.0013515773100290511 16 1.158494837167758E-4 17 3.8616494572258606E-5 18 3.0893195657806885E-4 19 6.564804077283963E-4 20 1.93082472861293E-4 21 3.0893195657806885E-4 22 0.0032437855440697228 23 7.723298914451721E-5 24 0.0 25 0.0018149752448961545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2589567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.7541671896209 #Duplication Level Percentage of deduplicated Percentage of total 1 91.51130545539012 72.98407955029651 2 6.630190586822546 10.57570657120992 3 0.9355627338387178 2.238450800728557 4 0.3012025138552396 0.9608862259177955 5 0.14077245388826476 0.5613594911548934 6 0.09258781181944478 0.4430558294141495 7 0.06349410583133737 0.3544743672419575 8 0.046493895112786315 0.29664655072974866 9 0.03724679335484029 0.26735282860492743 >10 0.20763302761217026 3.1215931317494947 >50 0.01777730536685501 0.9896468087060226 >100 0.012817103452792796 1.8902360126346958 >500 0.0016525210711254013 0.929041574522816 >1k 9.720712182801224E-4 1.7824170600732547 >5k 1.4581068274201835E-4 0.8914023468627849 >10k+ 1.4581068274201835E-4 1.7136508501526346 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 18258 0.7050599579002976 No Hit GGTATCAACGCAGAGTACTTTTTTT 14848 0.5733777114088958 No Hit TATCAACGCAGAGTACTTTTTTTTT 11102 0.4287203227412151 No Hit GTACATGGGAAGCAGTGGTATCAAC 7934 0.3063832679362998 No Hit CCCATGTACTCTGCGTTGATACCAC 7747 0.2991619834512874 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 7315 0.2824796577960717 No Hit GAGTACATGGGAAGCAGTGGTATCA 4051 0.15643541951221962 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3208 0.12388171458780561 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3188 0.12310938469636044 No Hit ATTCCATTCCATTCCATTCCATTCC 3154 0.12179642388090363 No Hit CATGTACTCTGCGTTGATACCACTG 3082 0.11901603627170103 No Hit GAATGGAATGGAATGGAATGGAATG 2690 0.10387837039937564 No Hit GGTATCAACGCAGAGTACATGGGAA 2606 0.10063458485530592 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.8616494572258606E-5 0.0 10 0.0 0.0 0.0 7.723298914451721E-5 0.0 11 0.0 0.0 0.0 7.723298914451721E-5 0.0 12 0.0 0.0 0.0 7.723298914451721E-5 0.0 13 0.0 0.0 0.0 7.723298914451721E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCTA 55 0.0030227762 12.115639 10 CGCAGCG 70 0.001466053 10.879983 8 CGCGGTG 70 0.0015139028 10.838176 14 TTCCGTA 130 3.73484E-7 10.252292 7 ACTATCG 165 1.0417352E-8 9.77701 11 GTCGCCC 205 4.1836756E-11 9.7209 11 TATTCCG 130 4.1288113E-6 9.519985 5 TGAACCG 80 0.0044395267 9.519983 5 CCGTACC 110 6.688202E-5 9.519431 9 CGGTCAG 170 1.7131242E-8 9.48377 16 CGCCCTA 110 6.968529E-5 9.48377 16 TCTAGTA 165 1.06960215E-7 9.210256 2 GGTATCA 5945 0.0 9.115641 1 TAGACAG 410 0.0 9.055594 5 GTCTAGT 200 2.6211637E-9 9.03208 1 CTAGGAC 265 1.8189894E-12 8.960449 3 TCCGTAT 85 0.0073104296 8.959986 8 CAGCGTA 85 0.007313918 8.959465 10 CTATCGG 180 4.3424734E-8 8.956548 12 CGCGCCT 85 0.0075449697 8.925556 12 >>END_MODULE