FastQCFastQC Report
Wed 25 May 2016
SRR1294637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801722
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC178590.4697608083915657No Hit
CCCATGTACTCTGCGTTGATACCAC168080.44211544137104186No Hit
GAGTACATGGGAAGCAGTGGTATCA84380.22195205225421533No Hit
ATTCCATTCCATTCCATTCCATTCC82480.2169543170173937No Hit
CATGTACTCTGCGTTGATACCACTG64400.16939692065858575No Hit
GAATGGAATGGAATGGAATGGAATG60200.15834929539824322No Hit
GTATCAACGCAGAGTACTTTTTTTT55850.14690711209288843No Hit
GCGTTGATACCACTGCTTCCCATGT53450.1405941833726927No Hit
GTATCAACGCAGAGTACATGGGAAG50870.13380778499848225No Hit
ACGCAGAGTACATGGGAAGCAGTGG43850.11534246849190972No Hit
GGTATCAACGCAGAGTACTTTTTTT38670.10171706400415391No Hit
GGTATCAACGCAGAGTACATGGGAA38020.10000731247576755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGA401.2900788E-518.9930386
AACGCGG402.7698895E-416.6191275
CGCGGAG508.746522E-515.1944297
CGGAGTA701.0954424E-412.2098089
GTCCCGC3400.011.4614912
TGTCCCG3450.011.28527311
TACACCG855.3465308E-511.1725225
ATTTCGA600.005885709511.0814619
CCTACGC600.00589205611.0798553
CGCTCCA951.3670597E-510.99596910
TAGACGT700.001496338510.8535934
GCGCCTT1803.45608E-1110.56086213
CGCCTTT2001.8189894E-1210.45525414
CGCCCTA3600.010.29724716
TTAGACA5400.010.2003684
CGCGCCT1909.822543E-1110.00489512
CGCGCGC1901.0004442E-109.99633610
GCGCGCC1901.0004442E-109.9959411
GTCTAGG4350.09.8263131
TCGCGCG1856.548362E-109.7531819
Produced by FastQC (version 0.11.5)