##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294637_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3801722 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.060759308544917 31.0 30.0 34.0 25.0 34.0 2 30.258419474122515 31.0 30.0 34.0 25.0 34.0 3 30.16217045854484 31.0 30.0 34.0 25.0 34.0 4 33.72055505373618 35.0 35.0 37.0 28.0 37.0 5 34.10962795280665 35.0 35.0 37.0 29.0 37.0 6 34.22712286695345 37.0 35.0 37.0 30.0 37.0 7 34.10216133636284 37.0 35.0 37.0 29.0 37.0 8 33.93055462761349 37.0 35.0 37.0 28.0 37.0 9 35.09327983476961 39.0 35.0 39.0 27.0 39.0 10 35.187766754118265 39.0 35.0 39.0 27.0 39.0 11 35.450656833929465 39.0 35.0 39.0 28.0 39.0 12 35.210091900459844 39.0 35.0 39.0 27.0 39.0 13 35.09401239753985 38.0 35.0 39.0 27.0 39.0 14 35.984565678395214 39.0 35.0 41.0 27.0 41.0 15 35.975583696019854 39.0 35.0 41.0 27.0 41.0 16 36.08303474057283 39.0 35.0 41.0 27.0 41.0 17 36.04704999471292 39.0 35.0 41.0 27.0 41.0 18 35.93525013138783 39.0 35.0 41.0 27.0 41.0 19 36.21016791864319 39.0 35.0 41.0 27.0 41.0 20 36.32116840736908 39.0 36.0 41.0 27.0 41.0 21 36.35477449429495 39.0 36.0 41.0 27.0 41.0 22 36.34325418849669 39.0 36.0 41.0 27.0 41.0 23 36.31387539646508 39.0 36.0 41.0 27.0 41.0 24 36.32174788161786 39.0 36.0 41.0 27.0 41.0 25 35.05283526780759 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0062037648503868 1101 2 0.9940667336242441 1101 3 1.2654687090170746 1101 4 1.0437227455069618 1101 5 0.7860854948468372 1101 6 0.78110416889254 1101 7 0.7769550496734396 1101 8 0.9275024300148829 1101 9 1.2163667576493253 1101 10 1.1567468347720649 1101 11 0.9370888358564358 1101 12 1.1391220616766233 1101 13 1.162848322667628 1101 14 1.2642253427724626 1101 15 1.4174629779376673 1101 16 1.2430263385703029 1101 17 1.251513923607888 1101 18 1.2723076328283511 1101 19 1.1041590968056525 1101 20 1.0619702162298736 1101 21 0.9806915151944722 1101 22 0.9338892021130292 1101 23 0.9743686758375247 1101 24 1.0896533108625377 1101 25 0.95470417447887 1102 1 0.8772848109823492 1102 2 0.7649613924920757 1102 3 1.1027223060571743 1102 4 0.9933165282877638 1102 5 0.7963337287940604 1102 6 0.6610723314507752 1102 7 0.6186155015733803 1102 8 0.7070433621964511 1102 9 1.0398087462854946 1102 10 0.941501649683488 1102 11 0.73904735131309 1102 12 1.016585645373091 1102 13 0.9981068959801433 1102 14 1.172265021410503 1102 15 1.322996071152481 1102 16 1.1508040494626997 1102 17 1.2385269004867254 1102 18 1.3401190985434681 1102 19 1.0130787641177648 1102 20 0.19349864753356627 1102 21 0.6129059118270135 1102 22 0.6453776025156799 1102 23 0.7610078191695493 1102 24 0.9146745191023697 1102 25 0.8053006248716557 1103 1 0.6235653202293108 1103 2 0.6329188926599763 1103 3 0.9022085301126062 1103 4 0.6890035944426813 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8 0.36891967543461135 2207 9 -1.3044569155476466 2207 10 -0.12370763362488901 2207 11 0.1915292206385928 2207 12 0.4995116688271608 2207 13 0.6919470405853758 2207 14 0.824006399634321 2207 15 0.8608644661384588 2207 16 0.8402690212196546 2207 17 0.8430619091082576 2207 18 0.7774294736475511 2207 19 -0.27710951666040984 2207 20 0.31007084369247195 2207 21 -0.3336141909861752 2207 22 0.26796440185204773 2207 23 -0.41533800661458287 2207 24 -1.6266873357774614 2207 25 -0.20502515143975586 2208 1 0.45905556187316776 2208 2 0.13712721670616546 2208 3 0.6246123545641495 2208 4 0.5020887635835365 2208 5 0.3858628699145399 2208 6 0.29641594305726926 2208 7 0.33858954207441627 2208 8 0.5302985708045895 2208 9 -0.09683908391546225 2208 10 0.09374646042185475 2208 11 0.2898451310372465 2208 12 0.22420966529647757 2208 13 0.5779558349120038 2208 14 0.7893081535753055 2208 15 0.7038949100101206 2208 16 0.5412140723619316 2208 17 0.4761694930597713 2208 18 0.7574902419925635 2208 19 -1.2095336127241083 2208 20 -0.1263598415391698 2208 21 -0.25139256613027783 2208 22 0.24284787044725675 2208 23 0.32688681906050476 2208 24 0.45034746107339174 2208 25 0.5277858423791884 2209 1 0.1684353241407699 2209 2 -1.373988269884638 2209 3 -0.22610834088538212 2209 4 0.04446954000341918 2209 5 0.021109786325197888 2209 6 0.16930894729459567 2209 7 0.13279080275201238 2209 8 0.27004133755226434 2209 9 -2.0265086348024397 2209 10 -0.3372908474353977 2209 11 -0.09957518383546216 2209 12 0.1942086268692762 2209 13 0.301775534280587 2209 14 0.46205678945957374 2209 15 0.6852567600828152 2209 16 0.5661158937902115 2209 17 0.3871153923512125 2209 18 0.582860029964607 2209 19 0.2806997771607982 2209 20 0.20792808060846113 2209 21 0.3468782502892793 2209 22 0.2721499259936664 2209 23 0.32559195428530785 2209 24 0.4201503352241076 2209 25 0.4565323393731475 2210 1 0.30640113873885966 2210 2 0.2832262862338766 2210 3 0.2329201428613601 2210 4 0.41928012077096355 2210 5 0.2989627261389387 2210 6 0.3000110112828622 2210 7 0.33119934051730127 2210 8 0.4438772269096276 2210 9 0.5782246656437167 2210 10 -1.4028476854696876 2210 11 -0.2262414598533553 2210 12 -2.009306326348259 2210 13 -0.42567110809317654 2210 14 -0.19243980508242942 2210 15 -0.645367900938524 2210 16 0.10812669477464709 2210 17 0.2481759765255802 2210 18 0.5215802509446235 2210 19 0.3600863313932976 2210 20 0.4528049035982278 2210 21 0.35490846742640514 2210 22 0.32334728720660877 2210 23 0.25754425334420716 2210 24 0.22209419899488125 2210 25 0.1551093103552077 2211 1 -0.44951552567455977 2211 2 -0.201192852247015 2211 3 -1.6372850972946686 2211 4 -0.8374331976387452 2211 5 -0.6603079469468724 2211 6 -0.5457884462296363 2211 7 -0.578339196445711 2211 8 -0.24019915681062542 2211 9 0.00958982149502674 2211 10 0.07344798018041132 2211 11 -0.2733010409767189 2211 12 -0.38662634274242436 2211 13 -0.3040144079029119 2211 14 -0.926726594828672 2211 15 -0.5000857419748854 2211 16 -0.19627080539967778 2211 17 -0.2651989304389488 2211 18 -0.21794388419908728 2211 19 -0.2582518665861784 2211 20 -0.2694619882984739 2211 21 -0.3682234942237699 2211 22 -0.39458820772784975 2211 23 -0.6814329470958214 2211 24 -0.3989041729733671 2211 25 -0.17130703457120688 2212 1 -0.3278332706949669 2212 2 -0.36973905428690657 2212 3 -2.2626988228009424 2212 4 -1.3469490279676677 2212 5 -0.614933760383849 2212 6 -0.386978620837489 2212 7 -0.3364010724856499 2212 8 -0.3211219538743677 2212 9 -0.7917205133070055 2212 10 -0.2954116765719661 2212 11 -0.2675561465512004 2212 12 0.02943428772424994 2212 13 -0.44809146332295313 2212 14 -0.10036103340456748 2212 15 0.11257779315884164 2212 16 -1.0068847004461503 2212 17 -1.1988321137859757 2212 18 -0.220548522430704 2212 19 -0.11844408366138026 2212 20 -0.1280576201479633 2212 21 0.12054423808725545 2212 22 -0.1551086234737582 2212 23 0.012229135635415389 2212 24 -0.12686071017546396 2212 25 0.14403251365214942 2213 1 -3.074522361149004 2213 2 -1.2700237311665745 2213 3 -2.357666213639064 2213 4 -0.9471267636204459 2213 5 -0.7623669002540581 2213 6 -0.6058310931662376 2213 7 -0.33594532295799695 2213 8 -0.20904063523240524 2213 9 -0.2967971815880617 2213 10 -0.09761305006711751 2213 11 -0.4046830755431472 2213 12 -0.10968816955860206 2213 13 -0.4680270903679187 2213 14 -0.16456312400561046 2213 15 -0.19651033715620514 2213 16 -1.434428812661146 2213 17 -0.872440076742997 2213 18 -0.3761779910685661 2213 19 -0.10498864521276374 2213 20 -0.19401545415502142 2213 21 0.08856847809867929 2213 22 0.013056081007405851 2213 23 0.09088544910313345 2213 24 0.06348967642456671 2213 25 0.05999361669593384 2214 1 -0.8123101017320877 2214 2 -0.683977624545065 2214 3 -6.334643347680899 2214 4 -1.9811783528601055 2214 5 -1.2450523271603657 2214 6 -0.6640902128730488 2214 7 -0.5887573363468945 2214 8 -0.24113157958741027 2214 9 -0.15785654127651583 2214 10 -0.09935636594401842 2214 11 -0.7285841993606397 2214 12 -0.38581595765260346 2214 13 -4.869221122506556 2214 14 -3.717645478670029 2214 15 -2.2625321560688576 2214 16 -2.8037049886925303 2214 17 -3.415450398593464 2214 18 -1.0679052670087046 2214 19 -0.695698639153612 2214 20 -0.2646053976639564 2214 21 -0.3858199966510867 2214 22 -0.1837048907223675 2214 23 -0.21163972320802316 2214 24 -0.14399267517408276 2214 25 -0.015487592051897536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1531.0 3 178.0 4 185.0 5 438.0 6 61.0 7 82.0 8 265.0 9 619.0 10 1481.0 11 3065.0 12 5590.0 13 9208.0 14 13554.0 15 17979.0 16 21449.0 17 23636.0 18 25301.0 19 25845.0 20 26849.0 21 27557.0 22 28458.0 23 30256.0 24 32032.0 25 34946.0 26 38858.0 27 43985.0 28 51035.0 29 62513.0 30 76904.0 31 95719.0 32 124759.0 33 164563.0 34 220190.0 35 299164.0 36 417027.0 37 629035.0 38 1247405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.471050604107496 22.104415306198216 12.711403394671805 22.71313069502248 2 14.156566576377315 22.221154868518987 41.7436564976224 21.878622057481294 3 17.727870527382677 28.791797526909203 30.096403851311358 23.383928094396765 4 11.490042940051309 17.408274723689875 40.088943489460796 31.012738846798023 5 12.615952738863696 38.47957168542543 35.66980997014599 13.234665605564889 6 29.668606871710885 40.00481168745195 17.973337250067978 12.353244190769193 7 24.226385018565708 33.733311328752905 25.327008858949323 16.713294793732064 8 23.111115322377724 38.2757811109953 22.52359098915283 16.089512577474146 9 23.941125437205592 16.74215767407679 23.098616314059438 36.21810057465819 10 14.323850535381505 30.601632992556247 34.213668284568165 20.860848187494078 11 31.618420796355593 27.386390016418567 23.030154041814434 17.96503514541141 12 19.54324434016842 28.79200951328633 33.53930889701783 18.125437249527415 13 25.545628954150086 25.255735812436452 27.741357623223667 21.45727761018979 14 19.184787229198346 23.643060210374262 29.565946143283284 27.606206417144108 15 22.90187048481202 30.794904717458405 24.545329157631684 21.757895640097896 16 20.8305718669969 32.62368693262724 26.60943697671419 19.936304223661665 17 19.084135632176043 31.275138602021958 30.200540273484826 19.440185492317173 18 19.413028806970274 31.01955780386562 30.557152167121416 19.010261222042686 19 19.856195097914313 29.355973462741602 30.80981596383925 19.978015475504836 20 21.732665722788962 29.003472027555123 29.20456588337451 20.059296366281405 21 21.79814566524378 30.44236406888191 28.343564127629357 19.415926138244956 22 19.795357495289032 29.830963174825282 30.341108569289727 20.03257076059596 23 19.612072652806603 30.50783527433002 29.885803854267976 19.9942882185954 24 19.941980991140518 29.062679746390597 29.834537894652325 21.16080136781656 25 21.054781716246517 28.88811149603464 28.420280687955824 21.636826099763017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3704.0 1 3704.0 2 3614.0 3 3524.0 4 3524.0 5 3524.0 6 5375.0 7 7226.0 8 7226.0 9 7226.0 10 11517.0 11 15808.0 12 15808.0 13 15808.0 14 27096.5 15 38385.0 16 38385.0 17 38385.0 18 61670.0 19 84955.0 20 84955.0 21 84955.0 22 121131.5 23 157308.0 24 157308.0 25 157308.0 26 205337.5 27 253367.0 28 253367.0 29 253367.0 30 304886.5 31 356406.0 32 356406.0 33 356406.0 34 401161.5 35 445917.0 36 445917.0 37 445917.0 38 475552.0 39 505187.0 40 505187.0 41 505187.0 42 489697.0 43 474207.0 44 474207.0 45 474207.0 46 476263.0 47 478319.0 48 478319.0 49 478319.0 50 426921.5 51 375524.0 52 375524.0 53 375524.0 54 316263.0 55 257002.0 56 257002.0 57 257002.0 58 212727.5 59 168453.0 60 168453.0 61 168453.0 62 134731.5 63 101010.0 64 101010.0 65 101010.0 66 74068.0 67 47126.0 68 47126.0 69 47126.0 70 32915.5 71 18705.0 72 18705.0 73 18705.0 74 12466.0 75 6227.0 76 6227.0 77 6227.0 78 4198.5 79 2170.0 80 2170.0 81 2170.0 82 1449.0 83 728.0 84 728.0 85 728.0 86 460.5 87 193.0 88 193.0 89 193.0 90 183.0 91 173.0 92 173.0 93 173.0 94 114.5 95 56.0 96 56.0 97 56.0 98 49.0 99 42.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10226944526717105 2 0.054869872126368 3 0.05747395522344874 4 0.052765562552969415 5 0.049924744628881336 6 0.06941591205248569 7 0.06610162447438292 8 0.06286624850528261 9 0.06823223791744898 10 0.06739051408808955 11 0.06218234789392807 12 0.06323450268062736 13 0.06289255237495009 14 0.06557554708103329 15 0.06839006113545389 16 0.06344493363796722 17 0.06668030960706754 18 0.14435563673514265 19 0.07065219392685736 20 0.06578597803837313 21 0.06333971815929729 22 0.06862679596246123 23 0.06725899473975215 24 0.07804358130341987 25 0.07099414423253463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3801722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.91668831022052 #Duplication Level Percentage of deduplicated Percentage of total 1 88.88161899523296 67.47598165768876 2 8.262738322727627 12.54559459670855 3 1.5436718938182765 3.5157137404874987 4 0.4785242812167429 1.4531191482401513 5 0.21372888359986247 0.8112794519571082 6 0.12003586638271711 0.5467635272534408 7 0.08629666164203069 0.4585949734863425 8 0.06038358750014367 0.3667297593041068 9 0.042659206076805775 0.29146910861648967 >10 0.2656388789702007 3.7217731627353063 >50 0.023720510079371596 1.2642811381199348 >100 0.01752182785038863 2.589362121197279 >500 0.001817943640458855 0.935245379537872 >1k 0.0013284971908005371 1.9053387598818021 >5k 2.4472316672641335E-4 1.1986629485372626 >10k+ 6.992090477897523E-5 0.9200905262480633 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 17859 0.4697608083915657 No Hit CCCATGTACTCTGCGTTGATACCAC 16808 0.44211544137104186 No Hit GAGTACATGGGAAGCAGTGGTATCA 8438 0.22195205225421533 No Hit ATTCCATTCCATTCCATTCCATTCC 8248 0.2169543170173937 No Hit CATGTACTCTGCGTTGATACCACTG 6440 0.16939692065858575 No Hit GAATGGAATGGAATGGAATGGAATG 6020 0.15834929539824322 No Hit GTATCAACGCAGAGTACTTTTTTTT 5585 0.14690711209288843 No Hit GCGTTGATACCACTGCTTCCCATGT 5345 0.1405941833726927 No Hit GTATCAACGCAGAGTACATGGGAAG 5087 0.13380778499848225 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4385 0.11534246849190972 No Hit GGTATCAACGCAGAGTACTTTTTTT 3867 0.10171706400415391 No Hit GGTATCAACGCAGAGTACATGGGAA 3802 0.10000731247576755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.6303869667482263E-5 0.0 5.260773933496453E-5 0.0 5 0.0 2.6303869667482263E-5 0.0 5.260773933496453E-5 0.0 6 0.0 2.6303869667482263E-5 0.0 5.260773933496453E-5 0.0 7 0.0 2.6303869667482263E-5 0.0 5.260773933496453E-5 0.0 8 0.0 2.6303869667482263E-5 0.0 5.260773933496453E-5 0.0 9 0.0 2.6303869667482263E-5 0.0 5.260773933496453E-5 0.0 10 2.6303869667482263E-5 2.6303869667482263E-5 0.0 7.891160900244678E-5 0.0 11 2.6303869667482263E-5 2.6303869667482263E-5 5.260773933496453E-5 7.891160900244678E-5 0.0 12 5.260773933496453E-5 5.260773933496453E-5 5.260773933496453E-5 7.891160900244678E-5 0.0 13 5.260773933496453E-5 5.260773933496453E-5 5.260773933496453E-5 7.891160900244678E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGGA 40 1.2900788E-5 18.993038 6 AACGCGG 40 2.7698895E-4 16.619127 5 CGCGGAG 50 8.746522E-5 15.194429 7 CGGAGTA 70 1.0954424E-4 12.209808 9 GTCCCGC 340 0.0 11.46149 12 TGTCCCG 345 0.0 11.285273 11 TACACCG 85 5.3465308E-5 11.172522 5 ATTTCGA 60 0.0058857095 11.08146 19 CCTACGC 60 0.005892056 11.079855 3 CGCTCCA 95 1.3670597E-5 10.995969 10 TAGACGT 70 0.0014963385 10.853593 4 GCGCCTT 180 3.45608E-11 10.560862 13 CGCCTTT 200 1.8189894E-12 10.455254 14 CGCCCTA 360 0.0 10.297247 16 TTAGACA 540 0.0 10.200368 4 CGCGCCT 190 9.822543E-11 10.004895 12 CGCGCGC 190 1.0004442E-10 9.996336 10 GCGCGCC 190 1.0004442E-10 9.99594 11 GTCTAGG 435 0.0 9.826313 1 TCGCGCG 185 6.548362E-10 9.753181 9 >>END_MODULE