FastQCFastQC Report
Wed 25 May 2016
SRR1294637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801722
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC189550.49858984954712626No Hit
GTACATGGGAAGCAGTGGTATCAAC176240.46357939901970735No Hit
GTATCAACGCAGAGTACTTTTTTTT144170.3792228889960918No Hit
GGTATCAACGCAGAGTACTTTTTTT110130.28968451664798217No Hit
TATCAACGCAGAGTACTTTTTTTTT86890.2285543235407534No Hit
GAGTACATGGGAAGCAGTGGTATCA85130.2239248424792765No Hit
ATTCCATTCCATTCCATTCCATTCC77890.20488084084001934No Hit
CATGTACTCTGCGTTGATACCACTG73980.19459602780003377No Hit
GAATGGAATGGAATGGAATGGAATG69370.18246994388332444No Hit
GCGTTGATACCACTGCTTCCCATGT58680.15435110720878592No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58350.153483079509759No Hit
ACGCAGAGTACATGGGAAGCAGTGG52770.13880552023530388No Hit
GTATCAACGCAGAGTACATGGGAAG48750.12823136462897602No Hit
GGTATCAACGCAGAGTACATGGGAA42570.111975573174472No Hit
GCTTCCCATGTACTCTGCGTTGATA39840.10479461675524933No Hit
ACTCTGCGTTGATACCACTGCTTCC39810.10471570514624688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA550.003110670312.069613
AACACCG752.0375328E-411.4225755
CGTGCAC951.3321816E-511.02163810
ACACCGT1408.110874E-910.8786436
ACTAGAC3450.010.1908233
CTAGACA5450.010.1301134
AAACGGG750.00268782910.11560719
TAGACAG6600.010.095715
ATCGATG856.4786983E-410.0787438
CTGTCGC1701.5097612E-910.0786099
TCGGAAC951.6747855E-49.98237513
TATTCCG3150.09.972095
TAGACTG5400.09.8713615
GTACTAG3100.09.8182241
CGCCCTA3700.09.73930516
TGTCCCG3800.09.73742711
TCTAGAC3550.09.6360883
CGCAGCG1002.7029464E-49.5188138
TAGGACA4000.09.5188134
TAGGACC2500.09.5188124
Produced by FastQC (version 0.11.5)