##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294637_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3801722 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34987487249199 31.0 31.0 34.0 28.0 34.0 2 31.47945799298318 31.0 31.0 34.0 28.0 34.0 3 31.583177833623814 31.0 31.0 34.0 28.0 34.0 4 35.180809380591214 37.0 35.0 37.0 32.0 37.0 5 35.29291436880445 37.0 35.0 37.0 32.0 37.0 6 34.9017434730893 37.0 35.0 37.0 32.0 37.0 7 34.75581986268328 37.0 35.0 37.0 32.0 37.0 8 34.74660851056442 37.0 35.0 37.0 32.0 37.0 9 36.24340785570328 39.0 35.0 39.0 31.0 39.0 10 35.9916153785048 39.0 35.0 39.0 30.0 39.0 11 36.18126101803341 39.0 35.0 39.0 31.0 39.0 12 36.11420088054834 39.0 35.0 39.0 30.0 39.0 13 35.99669202535062 39.0 35.0 39.0 30.0 39.0 14 37.06126039726208 40.0 36.0 41.0 30.0 41.0 15 37.1248515804154 40.0 36.0 41.0 31.0 41.0 16 37.149317072631824 40.0 36.0 41.0 31.0 41.0 17 37.18402502865806 40.0 36.0 41.0 31.0 41.0 18 37.19421330649637 40.0 36.0 41.0 31.0 41.0 19 37.20708247473119 40.0 37.0 41.0 31.0 41.0 20 37.286967852988724 40.0 37.0 41.0 31.0 41.0 21 37.15985808536237 40.0 37.0 41.0 30.0 41.0 22 37.12241189650374 40.0 36.0 41.0 30.0 41.0 23 37.14167290506776 40.0 37.0 41.0 30.0 41.0 24 37.08295346161555 40.0 36.0 41.0 30.0 41.0 25 35.9905866341621 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5080434170328161 1101 2 0.48913656165125374 1101 3 0.3989965002278524 1101 4 0.3946896354049727 1101 5 0.43638031147226997 1101 6 0.5426825393932617 1101 7 0.5558042133141683 1101 8 0.604028719217574 1101 9 0.7216415134948946 1101 10 0.735916953868589 1101 11 0.7389698883029752 1101 12 0.8328205563322513 1101 13 0.793656120442904 1101 14 1.0481731171121496 1101 15 1.0173285896586464 1101 16 1.0018525965441896 1101 17 0.9671574675636379 1101 18 0.9875021528798626 1101 19 1.1214861195806876 1101 20 1.004168750666416 1101 21 1.2329231949277002 1101 22 1.1585361554893794 1101 23 1.1508633354620272 1101 24 1.2450818740818335 1101 25 1.1392020038721427 1102 1 0.37669708118124845 1102 2 0.35762931071848314 1102 3 0.360593533638216 1102 4 0.354242763021162 1102 5 0.3243964576034557 1102 6 0.4002566400356926 1102 7 0.4677978213011684 1102 8 0.4582189211779806 1102 9 0.5767960911861678 1102 10 0.7036788686633031 1102 11 0.6499157925208792 1102 12 0.7140007377476962 1102 13 0.7063931037115267 1102 14 0.9335040381127087 1102 15 0.8454341615453842 1102 16 0.8967902649420694 1102 17 0.7699129223452132 1102 18 0.7976493341626565 1102 19 0.8946839208352344 1102 20 0.8570260941797017 1102 21 1.0799810741929008 1102 22 1.0662458105057269 1102 23 0.9909467972710289 1102 24 0.9662953532698921 1102 25 0.997458335409604 1103 1 0.22594817314399407 1103 2 0.21265684436639276 1103 3 0.11065937246671709 1103 4 0.13194036541544563 1103 5 0.13801940623774556 1103 6 0.17224980750106056 1103 7 0.23698594973078713 1103 8 0.20556422019902953 1103 9 0.12514795548033675 1103 10 0.3781654358932087 1103 11 0.3062755801378003 1103 12 0.3167620899822552 1103 13 0.48278240525428373 1103 14 0.6227522483311603 1103 15 0.547988918141634 1103 16 0.5651135107884855 1103 17 0.5419645169917686 1103 18 0.5722604299652687 1103 19 0.7022421796536449 1103 20 0.543238191480583 1103 21 0.7944349288345123 1103 22 0.6934255050426756 1103 23 0.7435336648638184 1103 24 0.7659051754115467 1103 25 0.7780840221616003 1104 1 0.13268798448982722 1104 2 0.19016680870560165 1104 3 0.07157684014704557 1104 4 0.07437615965420719 1104 5 0.10950244707445478 1104 6 0.08623433550901183 1104 7 0.1474309165931018 1104 8 0.1276892422070901 1104 9 -0.1448277410996326 1104 10 0.2438180334686635 1104 11 0.2680353627836922 1104 12 0.3216945451391382 1104 13 -0.1665734417234006 1104 14 0.21307038502683184 1104 15 0.3963937390059016 1104 16 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3 0.24258521959716006 2207 4 0.20978721445098358 2207 5 0.2015495848525788 2207 6 0.3099812114625493 2207 7 0.3008453611304063 2207 8 0.3028762796470019 2207 9 0.3713002118361928 2207 10 0.549145552618107 2207 11 0.43334330469085103 2207 12 0.42043531198255124 2207 13 0.4501515052760112 2207 14 0.43324818951017363 2207 15 0.42647232158497417 2207 16 0.5914974505605954 2207 17 0.61311144793887 2207 18 0.493994085182905 2207 19 0.6961222660241191 2207 20 0.4928884370783422 2207 21 0.8311887207843682 2207 22 0.7727015264774479 2207 23 0.757669562016261 2207 24 0.8571503942262595 2207 25 0.8189690981180888 2208 1 0.1722155500632674 2208 2 0.15243996114815062 2208 3 0.1632970325424985 2208 4 0.1628580277693601 2208 5 0.14316477083400514 2208 6 0.2847212165757611 2208 7 0.2344201668720487 2208 8 0.19602868518573757 2208 9 0.2824222321905694 2208 10 0.33251589718005903 2208 11 -0.04471106606317221 2208 12 -0.1920328231709405 2208 13 0.05777039935082229 2208 14 -0.3665972672490909 2208 15 0.17473242844983616 2208 16 0.24909821565420032 2208 17 0.24815486980009638 2208 18 0.36988876305368024 2208 19 0.6127981461482079 2208 20 0.45846441926438786 2208 21 0.6996932332985963 2208 22 0.7583748587629557 2208 23 0.7350362625686699 2208 24 0.7570756859595136 2208 25 0.6727463345935334 2209 1 0.02053114022707092 2209 2 -0.02867246410531976 2209 3 0.06657407952526384 2209 4 -0.4372945670848978 2209 5 -0.03289091853331172 2209 6 0.13421130836194095 2209 7 0.13463783200220547 2209 8 0.16972384072055036 2209 9 0.200063896587082 2209 10 0.2930851980353424 2209 11 0.26236284837582247 2209 12 0.33342880492107696 2209 13 0.3703334804317535 2209 14 0.3591400834938554 2209 15 -0.27884373951104635 2209 16 -0.29231028272612747 2209 17 0.07506571071034784 2209 18 -0.3028574453384323 2209 19 0.19384718688422709 2209 20 0.21983600627784483 2209 21 0.5271288845273148 2209 22 0.513675759901993 2209 23 0.46297400009203216 2209 24 0.6065740042468875 2209 25 0.5741563029552168 2210 1 -0.09663058291560134 2210 2 -0.14093347563127523 2210 3 -0.015431638482645837 2210 4 0.01856700424984581 2210 5 0.052163631480176775 2210 6 -0.4339431623407677 2210 7 -0.06422074678120282 2210 8 -0.11545369552120377 2210 9 0.05341075726991562 2210 10 0.2857196516221805 2210 11 0.18397013188599232 2210 12 0.20097100180260696 2210 13 0.26625926352356544 2210 14 0.3423805696062914 2210 15 0.1252329303080657 2210 16 0.2006302396348829 2210 17 -0.834790766746039 2210 18 -1.2638028789722 2210 19 -0.16697764529578762 2210 20 -0.05944445955719857 2210 21 -0.21648898193076604 2210 22 -0.1685064314196083 2210 23 -0.025006327562358877 2210 24 0.3265477853545704 2210 25 0.3048679741779381 2211 1 -0.21530886733445342 2211 2 -0.203966410542737 2211 3 -0.1477526937432252 2211 4 -0.15693471130283854 2211 5 -0.05176257200386658 2211 6 -0.029742121766631158 2211 7 -0.1956308015849686 2211 8 -0.12326764308549087 2211 9 -0.13649672669524904 2211 10 -0.03256197794652138 2211 11 -0.32184986814247196 2211 12 -0.18288832287730372 2211 13 -0.14806766489000012 2211 14 -0.21804836837338115 2211 15 -0.27337808395286345 2211 16 -0.27292698126938575 2211 17 -0.47314692430649075 2211 18 -0.42825322613858674 2211 19 -0.1478302699988916 2211 20 -0.37068111617200117 2211 21 -0.020952371851485907 2211 22 -0.15711006688280804 2211 23 -0.7728031480618966 2211 24 -0.22649730287080416 2211 25 -0.7917172150024783 2212 1 -0.19021091848418692 2212 2 -0.3100169279108371 2212 3 -0.14221623781221737 2212 4 -0.08409718213582096 2212 5 -0.035140291752639996 2212 6 -0.09719132427098742 2212 7 -0.18095194208377308 2212 8 -0.3176414819700426 2212 9 -0.09046231886403433 2212 10 -0.4721739742868891 2212 11 -0.1598473704056005 2212 12 -0.24888401172552932 2212 13 -0.18198647027993786 2212 14 -0.2784703965519668 2212 15 -0.1718391105139787 2212 16 -0.4586457948282785 2212 17 -0.5618908011383468 2212 18 -0.37508761631384147 2212 19 -0.4747844785904931 2212 20 -0.6335952794374293 2212 21 -0.14769841782305804 2212 22 -0.4248460562334415 2212 23 -0.33810669849746233 2212 24 -0.4608266937790404 2212 25 -0.13577805513375552 2213 1 -0.1844964757133596 2213 2 -0.22092489140923277 2213 3 -0.1315750027243361 2213 4 -0.05576133837661956 2213 5 -0.12894798660700957 2213 6 -0.2798209957140543 2213 7 -0.4066724315314474 2213 8 -0.31299849201327845 2213 9 -0.255659904024526 2213 10 -0.2806804659275244 2213 11 -0.007011408169844913 2213 12 0.03359819909277917 2213 13 0.10086895209418145 2213 14 -0.6804430207639101 2213 15 -0.1721053808429147 2213 16 -0.35361701689415526 2213 17 -0.22062177357474866 2213 18 -0.19396636586773042 2213 19 -0.2971403588379786 2213 20 -0.33542893835571164 2213 21 -0.15100934819090384 2213 22 -0.4073829036910297 2213 23 -0.4650544301399435 2213 24 -0.6439425979966771 2213 25 -0.33460436683994743 2214 1 -0.3116519992297313 2214 2 -0.21162700802515033 2214 3 -0.1594560491566952 2214 4 -0.13507335410742627 2214 5 -0.19599364485969772 2214 6 -0.208389115200724 2214 7 -0.23196813992225174 2214 8 -0.15107288206348812 2214 9 -0.19603783678452658 2214 10 -0.18676061110875253 2214 11 -0.0905505667778641 2214 12 -0.2747412906183726 2214 13 -0.26599987085889865 2214 14 -0.0832845208961217 2214 15 -0.025631079870343854 2214 16 -0.18544515665748662 2214 17 -0.4424675117077683 2214 18 -0.3506143384359035 2214 19 -0.4784761232131345 2214 20 -0.5366967192877325 2214 21 -0.636474108232683 2214 22 -0.9543084263515382 2214 23 -1.2617193836658558 2214 24 -0.771477808574609 2214 25 -1.0395805368675397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 22.0 9 55.0 10 136.0 11 253.0 12 615.0 13 1560.0 14 3048.0 15 5364.0 16 8059.0 17 10400.0 18 12290.0 19 13749.0 20 15234.0 21 16692.0 22 18367.0 23 20512.0 24 23701.0 25 26349.0 26 30541.0 27 34677.0 28 41271.0 29 51448.0 30 65176.0 31 85494.0 32 114735.0 33 154565.0 34 210635.0 35 294272.0 36 421559.0 37 667197.0 38 1453744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.680377819606775 22.1465333229068 11.759390395426228 24.413698462060193 2 14.035560727480862 22.634742887565164 40.292109733431325 23.037586651522652 3 17.3862482806795 28.570141691500567 30.241840108856493 23.80176991896344 4 11.258087725598054 17.64455966863837 40.05469203177346 31.042660573990112 5 12.968095299985634 37.99844351916214 35.663880120456255 13.369581060395982 6 30.16434990985786 39.89191362034126 17.47023783686233 12.473498632938538 7 24.35707603114618 34.02937607671414 24.89849815149822 16.715049740641465 8 23.139226732425026 38.61625940841155 22.047957543659056 16.196556315504367 9 23.867641736144137 16.13867646996932 23.280774556305158 36.71290723758138 10 14.21835065801392 30.500504661317063 34.244030022991005 21.037114657678007 11 31.846126189011624 26.881381655260526 22.89529906937361 18.377193086354243 12 19.598307583650847 28.712629860863597 33.458050617295314 18.23101193819024 13 25.88958465620017 24.79297517595992 27.75376816481391 21.563672003026 14 19.238651383929454 23.22452603128884 29.89548028171793 27.64134230306378 15 23.129667020545675 30.298768227487034 24.724752977371033 21.846811774596254 16 20.84334414773095 32.285238110519394 26.703793701906665 20.167624039842995 17 18.88983962789484 31.250215362989554 30.286362413233377 19.573582595882232 18 19.21980857025316 30.803075880630498 30.88880050756134 19.088315041555006 19 19.72296121793742 29.044092310136556 31.39869311303925 19.834253358886773 20 21.56631579833396 28.49572205777872 29.934889843020684 20.003072300866638 21 21.653282338737043 30.103716485476273 28.81629582477359 19.426705351013098 22 19.55343060455534 29.65345680402272 30.731843716494406 20.06126887492753 23 19.35454427565229 30.289314012589575 30.46334064318833 19.892801068569806 24 19.734930574863334 28.769128507852106 30.541957182023616 20.95398373526095 25 20.866845115591975 28.499666460440316 29.085175692226105 21.548312731741603 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1712.0 1 1712.0 2 2005.5 3 2299.0 4 2299.0 5 2299.0 6 4608.5 7 6918.0 8 6918.0 9 6918.0 10 10797.5 11 14677.0 12 14677.0 13 14677.0 14 24392.5 15 34108.0 16 34108.0 17 34108.0 18 56923.5 19 79739.0 20 79739.0 21 79739.0 22 116783.5 23 153828.0 24 153828.0 25 153828.0 26 202399.5 27 250971.0 28 250971.0 29 250971.0 30 303775.0 31 356579.0 32 356579.0 33 356579.0 34 402485.0 35 448391.0 36 448391.0 37 448391.0 38 477043.5 39 505696.0 40 505696.0 41 505696.0 42 490735.0 43 475774.0 44 475774.0 45 475774.0 46 479201.0 47 482628.0 48 482628.0 49 482628.0 50 432339.5 51 382051.0 52 382051.0 53 382051.0 54 321940.0 55 261829.0 56 261829.0 57 261829.0 58 215851.0 59 169873.0 60 169873.0 61 169873.0 62 136207.5 63 102542.0 64 102542.0 65 102542.0 66 74139.0 67 45736.0 68 45736.0 69 45736.0 70 31870.0 71 18004.0 72 18004.0 73 18004.0 74 11868.5 75 5733.0 76 5733.0 77 5733.0 78 3748.0 79 1763.0 80 1763.0 81 1763.0 82 1137.5 83 512.0 84 512.0 85 512.0 86 308.0 87 104.0 88 104.0 89 104.0 90 123.0 91 142.0 92 142.0 93 142.0 94 109.5 95 77.0 96 77.0 97 77.0 98 56.5 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09782409129336653 2 0.0 3 0.1358331829628784 4 0.01149479104468975 5 0.0220952505206851 6 0.013230846442743579 7 0.0212009189519907 8 0.019333344205599463 9 2.6303869667482265E-4 10 0.39518933788425353 11 0.06452339229433399 12 0.001499320571046489 13 1.0521547866992904E-4 14 0.0012362818743716663 15 0.001209978004704184 16 0.0 17 2.630386966748226E-5 18 3.682541753447517E-4 19 4.2086191467971616E-4 20 2.8934256634230486E-4 21 3.1564643600978713E-4 22 0.003103856620762907 23 1.3151934833741132E-4 24 2.630386966748226E-5 25 0.0019464863553936874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3801722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.85460198735404 #Duplication Level Percentage of deduplicated Percentage of total 1 88.01609343329827 65.0039354899801 2 8.911653661519216 13.163332684412962 3 1.6687041903790751 3.6972445144522936 4 0.5193674398766696 1.5343070222912985 5 0.2350696038020748 0.868048601406362 6 0.13085138684251416 0.5798386256848307 7 0.0856798628661685 0.4429496519218365 8 0.060647884108601675 0.3583300273772761 9 0.04879784733468304 0.32435510334684126 >10 0.2749900874680448 3.784829425283983 >50 0.024714016991334915 1.2616695608007096 >100 0.019638110043149137 2.817977885463079 >500 0.0018604597048964369 0.9226909916276905 >1k 0.0015026789284599243 2.1141643170564426 >5k 2.8622455780188944E-4 1.4878157234868123 >10k+ 1.4311227890094472E-4 1.6385103754074832 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 18955 0.49858984954712626 No Hit GTACATGGGAAGCAGTGGTATCAAC 17624 0.46357939901970735 No Hit GTATCAACGCAGAGTACTTTTTTTT 14417 0.3792228889960918 No Hit GGTATCAACGCAGAGTACTTTTTTT 11013 0.28968451664798217 No Hit TATCAACGCAGAGTACTTTTTTTTT 8689 0.2285543235407534 No Hit GAGTACATGGGAAGCAGTGGTATCA 8513 0.2239248424792765 No Hit ATTCCATTCCATTCCATTCCATTCC 7789 0.20488084084001934 No Hit CATGTACTCTGCGTTGATACCACTG 7398 0.19459602780003377 No Hit GAATGGAATGGAATGGAATGGAATG 6937 0.18246994388332444 No Hit GCGTTGATACCACTGCTTCCCATGT 5868 0.15435110720878592 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5835 0.153483079509759 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5277 0.13880552023530388 No Hit GTATCAACGCAGAGTACATGGGAAG 4875 0.12823136462897602 No Hit GGTATCAACGCAGAGTACATGGGAA 4257 0.111975573174472 No Hit GCTTCCCATGTACTCTGCGTTGATA 3984 0.10479461675524933 No Hit ACTCTGCGTTGATACCACTGCTTCC 3981 0.10471570514624688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6303869667482263E-5 0.0 4 0.0 0.0 0.0 2.6303869667482263E-5 0.0 5 0.0 0.0 0.0 2.6303869667482263E-5 0.0 6 0.0 0.0 0.0 2.6303869667482263E-5 0.0 7 0.0 0.0 0.0 2.6303869667482263E-5 0.0 8 0.0 0.0 2.6303869667482263E-5 2.6303869667482263E-5 0.0 9 0.0 0.0 2.6303869667482263E-5 2.6303869667482263E-5 0.0 10 0.0 0.0 2.6303869667482263E-5 5.260773933496453E-5 0.0 11 0.0 0.0 2.6303869667482263E-5 5.260773933496453E-5 0.0 12 0.0 0.0 2.6303869667482263E-5 5.260773933496453E-5 0.0 13 0.0 0.0 2.6303869667482263E-5 5.260773933496453E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGGA 55 0.0031106703 12.0696 13 AACACCG 75 2.0375328E-4 11.422575 5 CGTGCAC 95 1.3321816E-5 11.021638 10 ACACCGT 140 8.110874E-9 10.878643 6 ACTAGAC 345 0.0 10.190823 3 CTAGACA 545 0.0 10.130113 4 AAACGGG 75 0.002687829 10.115607 19 TAGACAG 660 0.0 10.09571 5 ATCGATG 85 6.4786983E-4 10.078743 8 CTGTCGC 170 1.5097612E-9 10.078609 9 TCGGAAC 95 1.6747855E-4 9.982375 13 TATTCCG 315 0.0 9.97209 5 TAGACTG 540 0.0 9.871361 5 GTACTAG 310 0.0 9.818224 1 CGCCCTA 370 0.0 9.739305 16 TGTCCCG 380 0.0 9.737427 11 TCTAGAC 355 0.0 9.636088 3 CGCAGCG 100 2.7029464E-4 9.518813 8 TAGGACA 400 0.0 9.518813 4 TAGGACC 250 0.0 9.518812 4 >>END_MODULE