FastQCFastQC Report
Wed 25 May 2016
SRR1294636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182915
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC174630.4174839794736446No Hit
CCCATGTACTCTGCGTTGATACCAC166560.3981912135436651No Hit
GTATCAACGCAGAGTACTTTTTTTT132930.3177927354488437No Hit
GGTATCAACGCAGAGTACTTTTTTT88060.21052304433630617No Hit
GAGTACATGGGAAGCAGTGGTATCA79110.18912648236935248No Hit
TATCAACGCAGAGTACTTTTTTTTT77280.184751542883372No Hit
CATGTACTCTGCGTTGATACCACTG63500.1518080094862076No Hit
GCGTTGATACCACTGCTTCCCATGT52150.12467382196386971No Hit
GTATACGGTGCTACATTCTAAAGCA51380.12283300043151725No Hit
ACAGTATACCATGTGACATGAGGAC49550.11845806094553679No Hit
GTATCAACGCAGAGTACATGGGAAG48590.1161630107233831No Hit
ATAGTATACGGTGCTACATTCTAAA44190.10564403053851203No Hit
GGTATCAACGCAGAGTACATGGGAA44070.1053571492607428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC551.9661197E-413.8142668
GTCGCGT1051.366061E-913.57667815
CGCGTCA1102.732122E-912.96048717
TCGCGTC1155.298716E-912.3968416
GCGCTAT3300.011.79841611
ATCGCCG907.4845066E-611.608932519
GTGCGCT3550.011.5037829
TTGTGCG3750.011.1436417
ACTCGCC600.005863141711.08722312
TGCGCTA3700.010.78072810
TGTGCGC4050.010.5525658
CTGCGTG1252.5822155E-69.87729
CGTCGCG1451.6105878E-79.8312714
CGCCAGT5300.09.6802091
CCGTCCC1002.7651968E-49.4973099
GTATTAG5850.09.419741
TCGTCGC1554.241938E-79.19710413
GGCGCCC1151.11491856E-49.0843828
TAAGACT5250.09.045384
GCGTGCC1054.5130937E-49.04408211
Produced by FastQC (version 0.11.5)