##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294636_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4182915
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.96421108246283	31.0	30.0	33.0	25.0	34.0
2	30.11363415225985	31.0	30.0	34.0	25.0	34.0
3	30.045397288732858	31.0	30.0	34.0	25.0	34.0
4	33.64291959076386	35.0	35.0	37.0	28.0	37.0
5	34.040338137399395	35.0	35.0	37.0	28.0	37.0
6	34.129607223670575	37.0	35.0	37.0	29.0	37.0
7	34.04570712051285	37.0	35.0	37.0	29.0	37.0
8	33.93009300930093	37.0	35.0	37.0	28.0	37.0
9	34.975972975783634	39.0	35.0	39.0	27.0	39.0
10	35.15070375563453	39.0	35.0	39.0	27.0	39.0
11	35.34841922439256	39.0	35.0	39.0	28.0	39.0
12	35.14259194843787	39.0	35.0	39.0	27.0	39.0
13	34.977022483124806	38.0	35.0	39.0	27.0	39.0
14	35.873018218156474	39.0	35.0	41.0	27.0	41.0
15	35.8678875855713	39.0	35.0	41.0	27.0	41.0
16	35.9926223698067	39.0	35.0	41.0	27.0	41.0
17	36.00627839676398	39.0	35.0	41.0	27.0	41.0
18	35.90922359168188	39.0	35.0	41.0	27.0	41.0
19	36.20037198939018	39.0	35.0	41.0	27.0	41.0
20	36.31615583869144	39.0	36.0	41.0	27.0	41.0
21	36.36764074813856	39.0	36.0	41.0	27.0	41.0
22	36.383765866626504	39.0	36.0	41.0	27.0	41.0
23	36.37555365098263	39.0	36.0	41.0	27.0	41.0
24	36.39308018451248	39.0	36.0	41.0	27.0	41.0
25	35.224451369439734	38.0	33.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.0432954645153245
1101	2	1.0158704412976043
1101	3	1.2939685427804797
1101	4	1.0758701466593479
1101	5	0.8335569156073106
1101	6	0.8125771830856436
1101	7	0.7999999758840559
1101	8	0.9535108559550309
1101	9	1.1756103828570872
1101	10	1.1613767247785631
1101	11	0.8696385581219488
1101	12	1.1888940076609202
1101	13	1.2697064905434132
1101	14	1.3303492067631808
1101	15	1.4081834912384252
1101	16	1.4366486411532762
1101	17	1.3059823585108603
1101	18	1.346592314710179
1101	19	1.30040332514303
1101	20	1.1799138500977904
1101	21	1.0512039581770836
1101	22	1.0209569269510865
1101	23	1.0922844092863997
1101	24	1.0977152486153798
1101	25	1.1646187886393662
1102	1	0.8315513565201549
1102	2	0.8365897416379511
1102	3	1.0801474587888258
1102	4	0.9712718125018966
1102	5	0.7569915544039816
1102	6	0.6441055135132601
1102	7	0.5965564600502589
1102	8	0.7138856681174701
1102	9	0.9835320135964452
1102	10	0.8764972565908664
1102	11	0.629000128168343
1102	12	0.897052364746969
1102	13	0.9457268395922398
1102	14	1.144104886082303
1102	15	1.1452096870150186
1102	16	1.0505725406473445
1102	17	1.077829921592219
1102	18	1.1145733367489825
1102	19	0.9055182758637415
1102	20	0.13999556254960766
1102	21	0.34070138192399213
1102	22	0.5202552007016195
1102	23	0.7512303945081626
1102	24	0.8518813414511257
1102	25	0.8880298849679136
1103	1	0.7599751071274063
1103	2	0.7838148224906831
1103	3	1.0284830156583453
1103	4	0.8772257317664014
1103	5	0.5936213450018428
1103	6	0.4773533262240477
1103	7	0.47247325965781073
1103	8	0.5863343231772689
1103	9	0.7034836624075709
1103	10	0.7289521644525934
1103	11	0.6398363165136729
1103	12	0.8669201186216853
1103	13	0.7768667781254166
1103	14	0.6865958841762776
1103	15	0.8966953470335284
1103	16	0.9002615686003566
1103	17	0.9252819206936991
1103	18	1.0357581664602122
1103	19	0.8100200708436418
1103	20	-0.11624149616795165
1103	21	0.38292847873344726
1103	22	-0.12330366666431303
1103	23	0.4034468189793614
1103	24	0.5463855797260919
1103	25	0.6398040380833976
1104	1	0.3146237003912091
1104	2	0.3073417143455508
1104	3	0.5651623799683279
1104	4	0.454039103960163
1104	5	0.19261676234572178
1104	6	0.23601066091281098
1104	7	0.2246360109862806
1104	8	0.3291602951829091
1104	9	0.5980212121749346
1104	10	0.5369557116701529
1104	11	0.32834414366239173
1104	12	0.458688930915514
1104	13	0.41720032564584386
1104	14	0.6772427192419528
1104	15	0.6455516171984854
1104	16	0.5349165110527352
1104	17	0.5031022689121585
1104	18	0.5791373745785151
1104	19	-0.2747729683473068
1104	20	0.2702699973949976
1104	21	-0.43649978671057
1104	22	-0.09901897959065309
1104	23	-0.44055462723169114
1104	24	-0.8628966301639025
1104	25	-1.9384490821254374
1105	1	-0.2148610540187761
1105	2	-0.08199749811731039
1105	3	0.4907099825860257
1105	4	0.15871274863152962
1105	5	0.016426032899794052
1105	6	0.04352620223684056
1105	7	0.03781747198203078
1105	8	0.17653017383912584
1105	9	0.5135987745956498
1105	10	-1.5702634033143923
1105	11	-0.5749169769909912
1105	12	0.13831369677558314
1105	13	0.046738233599612045
1105	14	0.33175897470335514
1105	15	-0.40603770639633296
1105	16	-0.041488369301568184
1105	17	-0.22104032859067502
1105	18	-0.6338987209568003
1105	19	-1.7409039456435949
1105	20	-0.4934245970538882
1105	21	-1.6526455491234628
1105	22	-0.8897047727879581
1105	23	-0.4360974354000575
1105	24	-0.6491248829921403
1105	25	-0.6487464100679148
1106	1	-0.38712202173753596
1106	2	-0.34577377452438185
1106	3	0.06619152486861424
1106	4	0.1517870637161849
1106	5	-0.21425853097291991
1106	6	-0.48392375562179524
1106	7	-0.18613116616257486
1106	8	-0.10356279322966344
1106	9	0.19584162310504638
1106	10	-0.24239597524383072
1106	11	-0.3062380443922237
1106	12	-0.07604211945411521
1106	13	-0.12448738233100443
1106	14	-0.4953741666142548
1106	15	-0.7446614550766313
1106	16	-0.45123796515763814
1106	17	-0.27614864203966505
1106	18	-0.3067375112079702
1106	19	-0.41771352016986896
1106	20	-0.3700515912744464
1106	21	-0.3865269552174695
1106	22	-0.5746599443231304
1106	23	-0.7743231377759088
1106	24	-0.4967483109456765
1106	25	-0.6642925600719138
1107	1	0.47783032211590637
1107	2	0.5316770741778285
1107	3	0.6883871168749458
1107	4	0.5165994336966548
1107	5	0.37947854969446126
1107	6	0.3337560105951809
1107	7	0.2372910788307223
1107	8	0.44031896264182535
1107	9	0.6275131051714382
1107	10	-1.269424684919251
1107	11	-0.21284082412622496
1107	12	0.2312635892411663
1107	13	0.41777246759738773
1107	14	0.5237692593790158
1107	15	0.7209639786771334
1107	16	0.6291373673735876
1107	17	0.12958428482574647
1107	18	-0.13814745641027315
1107	19	0.2614623119604147
1107	20	0.3447334662107906
1107	21	0.4389972112237217
1107	22	0.40826393611509104
1107	23	0.4777147453585968
1107	24	0.4115021734193576
1107	25	0.25404926230902447
1108	1	-0.05729516100772969
1108	2	0.11149324623853474
1108	3	0.5198945898705709
1108	4	0.05042892831652068
1108	5	0.1432445539565279
1108	6	0.249270085249492
1108	7	0.14258435352348897
1108	8	0.23554376521537534
1108	9	0.4695400128101994
1108	10	0.48003701855565595
1108	11	0.19602104369496232
1108	12	0.04208002075879591
1108	13	0.29504769389166086
1108	14	0.5799440984674078
1108	15	0.0324993368113482
1108	16	0.32887171270675
1108	17	0.3225652681179909
1108	18	0.42036119886998335
1108	19	0.3246431359965385
1108	20	0.22209582619890256
1108	21	0.2568302911093525
1108	22	0.15138874007900682
1108	23	0.304934639695297
1108	24	0.3660336439415417
1108	25	0.03130154752122394
1109	1	-0.16728539309797696
1109	2	-0.201548603667991
1109	3	0.24748099860723372
1109	4	0.1630704585673044
1109	5	0.16726452429597316
1109	6	0.029198334383167435
1109	7	-0.04137762457578731
1109	8	-0.12041271447236568
1109	9	0.20694795783381892
1109	10	0.15023468704213627
1109	11	0.05701903275122788
1109	12	-0.050972473597155954
1109	13	0.07113903120118437
1109	14	0.3214712380049676
1109	15	-0.47800079997220024
1109	16	-0.1087902764840436
1109	17	0.008331417995378843
1109	18	0.20624181744684478
1109	19	0.1932977958273696
1109	20	0.20274956718646564
1109	21	0.19529859719818177
1109	22	0.048750616380161205
1109	23	0.013936122137167217
1109	24	0.20235009785105262
1109	25	-0.011037296469417868
1110	1	0.16962128013756583
1110	2	0.14231969862800398
1110	3	0.24914538992947755
1110	4	0.07293167761181962
1110	5	-0.03428514946118355
1110	6	0.00460968396757977
1110	7	-0.1391309695822187
1110	8	0.12498211209555876
1110	9	0.43630607231048657
1110	10	0.3208507472943509
1110	11	-0.13998276227959394
1110	12	0.14577573166153002
1110	13	0.17631758170001888
1110	14	0.21111852356686
1110	15	0.20852492272197765
1110	16	-0.1704322773610727
1110	17	0.14425542777587452
1110	18	-5.211910717140775
1110	19	-1.9683558639413476
1110	20	-0.6902127438056596
1110	21	-0.27973168063933684
1110	22	-0.262275139271118
1110	23	-0.3340202035403479
1110	24	-0.05448954208420531
1110	25	-0.13708080085711316
1111	1	-0.6088541486842871
1111	2	-0.5020649489265026
1111	3	-1.6590365357209507
1111	4	-0.9584689924922429
1111	5	-0.6649496007161346
1111	6	-0.7785885485040183
1111	7	-0.8789264579976503
1111	8	-2.235782941659348
1111	9	-0.6627959549792166
1111	10	-0.38611635673575506
1111	11	-0.7145374979452797
1111	12	-0.3837547994156907
1111	13	-1.0172731787152216
1111	14	-0.42917549334232774
1111	15	-0.3012570991372172
1111	16	-2.5027136400587082
1111	17	-2.132905354716904
1111	18	-0.9162881703379355
1111	19	-0.47568075192614145
1111	20	-0.40581862143231007
1111	21	-0.4540418744222734
1111	22	-0.44671071735788104
1111	23	-0.5503272512330781
1111	24	-0.5526822044989999
1111	25	-0.45049023736623184
1112	1	-0.3098745865624757
1112	2	-0.35185956993759504
1112	3	0.03531786718145469
1112	4	-0.4457969752359787
1112	5	-1.8816687978895175
1112	6	-0.9385964023249613
1112	7	-0.6304264247794222
1112	8	-0.247664247264062
1112	9	-0.4213530230279474
1112	10	-0.14438691422545702
1112	11	-0.7507341353536603
1112	12	-1.3878285806964215
1112	13	-1.6029904443392908
1112	14	-2.713805781077525
1112	15	-4.042255124665779
1112	16	-1.3101402287135144
1112	17	-0.686644474631521
1112	18	-0.24864504311955216
1112	19	-0.233597933894913
1112	20	-0.2996320448349792
1112	21	-0.16635209956400843
1112	22	-0.19676018760973335
1112	23	-0.13207568410673787
1112	24	-0.23845585987184847
1112	25	-0.14639978180301938
1113	1	-0.0620372727709082
1113	2	0.257420694424102
1113	3	-0.3369491752187592
1113	4	-2.268220553539141
1113	5	-0.3592771050339252
1113	6	-0.3083076015796138
1113	7	-0.052126894758643516
1113	8	-0.5628720874013453
1113	9	-2.288298313156446
1113	10	-0.46826229801381913
1113	11	-0.6209245047208896
1113	12	-1.6865636459016784
1113	13	-0.7379651459664487
1113	14	-0.2639660805616728
1113	15	0.4984908914300732
1113	16	0.23182846669033097
1113	17	0.6061538239067232
1113	18	0.5643014985208339
1113	19	0.5330482403682666
1113	20	0.7776178821547788
1113	21	0.5879963712267298
1113	22	0.6456733666065162
1113	23	0.713807281117127
1113	24	0.6860022813519322
1113	25	0.78833955870126
1201	1	1.0349193999254531
1201	2	1.1041671618637565
1201	3	1.3797347588598825
1201	4	1.2478223857882966
1201	5	0.9696961295508189
1201	6	0.9436905067275276
1201	7	0.9437522481846372
1201	8	1.1131158927500877
1201	9	1.42626302662174
1201	10	1.3214261842826005
1201	11	1.0839442258803018
1201	12	1.3163250160499658
1201	13	1.3523621070138034
1201	14	1.6037062352361247
1201	15	1.6500249419015773
1201	16	1.5284444555106873
1201	17	1.530233585140735
1201	18	1.5977262966249413
1201	19	1.3049784713747314
1201	20	1.2320916863478857
1201	21	1.1903396115446228
1201	22	1.1062455944300922
1201	23	1.1917466950506608
1201	24	1.2157587583303666
1201	25	1.2260771014527734
1202	1	0.7885370097865767
1202	2	0.8361253614509501
1202	3	1.0435775110631944
1202	4	0.9927941846965496
1202	5	0.7611698241758162
1202	6	0.6505387773799498
1202	7	0.6723274485916235
1202	8	0.8359655973133329
1202	9	1.1232777484053784
1202	10	0.8991532053641009
1202	11	0.7863604085435227
1202	12	0.9628330197428525
1202	13	1.0494302979815302
1202	14	1.1501129987366525
1202	15	1.3357664472776705
1202	16	1.1698920107298179
1202	17	1.1577037297241404
1202	18	1.2175685937547627
1202	19	1.1082090443516748
1202	20	1.1027082621462085
1202	21	0.8740464583516996
1202	22	0.8796671948486363
1202	23	0.8225975946696806
1202	24	0.913619620195071
1202	25	0.8514227241121546
1203	1	0.7477144809784875
1203	2	0.67713661725913
1203	3	0.9227737021397324
1203	4	0.8249386143370074
1203	5	0.6817609331857213
1203	6	0.5058440977430791
1203	7	0.608542402532116
1203	8	0.6324544728308794
1203	9	1.0195881164516791
1203	10	0.7183668747002585
1203	11	0.5723580303541027
1203	12	0.8243487573397346
1203	13	0.9107175765782998
1203	14	0.9141199136328098
1203	15	1.0289961526782605
1203	16	1.0610993000236633
1203	17	0.974205499039762
1203	18	1.004593134950447
1203	19	0.8395670478186759
1203	20	0.10430012090659346
1203	21	0.4896996247342287
1203	22	0.5314038939408121
1203	23	0.49969352930290256
1203	24	0.664047455517391
1203	25	0.800023460491289
1204	1	0.41689163643464155
1204	2	0.42280333944301773
1204	3	0.7503794029653506
1204	4	0.538084616700452
1204	5	0.3534553981186619
1204	6	0.2985078933762537
1204	7	0.24575496211399184
1204	8	0.37281517615477355
1204	9	-0.4558345678853257
1204	10	-0.3412919323139434
1204	11	0.05476906400546966
1204	12	0.3300288036569583
1204	13	0.49160807282295593
1204	14	-1.6109632432350622
1204	15	0.11160958625983142
1204	16	0.31114553793875643
1204	17	0.35646711205300363
1204	18	0.6511847740567731
1204	19	0.6548680701286713
1204	20	-0.634706755297934
1204	21	-0.552581339996479
1204	22	-1.6075737960668803
1204	23	-1.1005172775209573
1204	24	-0.1566482545875374
1204	25	-2.8168661474778105
1205	1	-0.30253958026807837
1205	2	-0.2096020366183211
1205	3	0.18492661752604178
1205	4	0.22726202071055468
1205	5	0.22015165525956348
1205	6	0.2189362930658234
1205	7	0.22839469473126428
1205	8	0.3992225151042206
1205	9	0.6375946833701107
1205	10	0.5685430136202569
1205	11	0.370913882665441
1205	12	0.4980602149850242
1205	13	0.5602639231316289
1205	14	0.7326763403101992
1205	15	0.15260230988224777
1205	16	-0.6035937491899332
1205	17	-0.8652790074362144
1205	18	-0.5217061289037446
1205	19	-0.541189870898954
1205	20	0.13059756593497696
1205	21	-0.19470557136391164
1205	22	0.18755468417275978
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2111	22	-0.08167360641020593
2111	23	-0.040829853463712595
2111	24	-0.10899750892065896
2111	25	-0.034399148094898635
2112	1	-1.0616984600229706
2112	2	-0.47030939687047635
2112	3	-0.26914491484652103
2112	4	-0.1568128323989697
2112	5	-0.7400860115371373
2112	6	-1.0439911706682068
2112	7	-0.5618313711259546
2112	8	-0.27330447522807333
2112	9	-2.433654108485584
2112	10	-0.8150381552830837
2112	11	-1.2330468240456085
2112	12	-0.9463433104257888
2112	13	-0.3408442154477669
2112	14	-0.79286412044992
2112	15	-0.949887623150218
2112	16	-1.0691087909676753
2112	17	-0.9978426596488248
2112	18	-0.1373128253822884
2112	19	-0.13088788449627486
2112	20	-0.09632754180700687
2112	21	-0.0778353029960357
2112	22	-0.041465605962855534
2112	23	-0.2264863317421586
2112	24	-0.2666228106137467
2112	25	-0.04763110797534864
2113	1	0.1061184725433435
2113	2	-0.2772314107015852
2113	3	-0.06787112948156349
2113	4	-0.05831533847928938
2113	5	-0.1777159149353622
2113	6	-0.6697069500118218
2113	7	-0.32826169030388286
2113	8	0.026670100078987957
2113	9	-0.8906260040795004
2113	10	-0.12855253755663654
2113	11	-0.25517956809966336
2113	12	-0.4991609051000978
2113	13	-0.13973286385852646
2113	14	0.034497519126155396
2113	15	-2.104926220524412
2113	16	-0.4616072946416949
2113	17	-1.5177163369318691
2113	18	-0.12246910666149802
2113	19	0.058679628596301825
2113	20	0.11555190009845973
2113	21	0.03898186554340555
2113	22	0.1268123593434467
2113	23	0.19881599019155516
2113	24	0.22503478736253157
2113	25	0.47241985054572666
2114	1	-0.584063971087847
2114	2	-0.4813055458636839
2114	3	-3.0040584786975515
2114	4	-1.2446530316499818
2114	5	-1.09411790982724
2114	6	-1.4992965528366256
2114	7	-3.3227556285325015
2114	8	-1.3147206355574923
2114	9	-0.7191838850913825
2114	10	-0.5854147709604618
2114	11	-0.6897931932493293
2114	12	-0.5591750209280875
2114	13	-3.0498832567502348
2114	14	-2.458549981174336
2114	15	-1.3783440860352414
2114	16	-1.7456304324552292
2114	17	-1.4878398983650598
2114	18	-0.5336240563370325
2114	19	-0.4894640686150922
2114	20	-0.12552167882970622
2114	21	-0.20186368194104176
2114	22	-0.28597436466739623
2114	23	-0.15631570601932765
2114	24	-0.28046967646595533
2114	25	-0.0882027319726717
2201	1	1.2283233676420053
2201	2	1.208924209342399
2201	3	1.4820938303780196
2201	4	1.238425925374976
2201	5	1.0787327788220011
2201	6	0.9735096498788423
2201	7	0.9537059316637695
2201	8	1.1140916230172806
2201	9	1.5419192816308538
2201	10	1.3417041551657505
2201	11	1.188648311470594
2201	12	1.360142728115136
2201	13	1.4303034045545289
2201	14	1.7397136089790735
2201	15	1.7472886660205944
2201	16	1.6146154195893345
2201	17	1.6687198079870953
2201	18	1.7271596567834635
2201	19	1.5512993009508733
2201	20	1.4252177698080004
2201	21	1.3584160632345075
2201	22	1.351691235669911
2201	23	1.236850312714509
2201	24	1.375021653612805
2201	25	1.5239571455069836
2202	1	1.049506407999285
2202	2	1.049679823880247
2202	3	1.3107045401543367
2202	4	1.1751765784030397
2202	5	0.8983420734230094
2202	6	0.8597452110463735
2202	7	0.9045611307639945
2202	8	0.989430613641062
2202	9	1.3155110061117554
2202	10	1.2124898952633316
2202	11	0.9587989599851099
2202	12	1.2260536668298911
2202	13	1.2742717027694184
2202	14	-0.6882122315282473
2202	15	0.7548760515345094
2202	16	1.1949184498448133
2202	17	1.2940619138358826
2202	18	1.3906788057812065
2202	19	1.355154926264099
2202	20	1.2564636460583287
2202	21	1.1301440670858938
2202	22	1.118338990226718
2202	23	1.0565377311948723
2202	24	1.1530884262714025
2202	25	1.2636858877216284
2203	1	0.7975437711851363
2203	2	0.8593790157080825
2203	3	1.1675895152574327
2203	4	0.854403313821571
2203	5	0.6664344093289074
2203	6	0.6885839662823514
2203	7	0.7031359619022339
2203	8	0.8251749532772763
2203	9	1.074306516224695
2203	10	0.8176953846543924
2203	11	0.7810949670354219
2203	12	0.8896289389257745
2203	13	1.0273601263489525
2203	14	0.816859782172088
2203	15	0.944699562145324
2203	16	1.006111831351987
2203	17	1.062864410469281
2203	18	1.1808829160068584
2203	19	1.0194535565433185
2203	20	0.19070197459742388
2203	21	0.6850545214098744
2203	22	-0.1920121934474679
2203	23	0.031649362281577
2203	24	0.417139619890591
2203	25	0.8081565290116473
2204	1	0.5922495812821751
2204	2	0.4471207259596355
2204	3	0.7119247730062739
2204	4	0.7024454958027491
2204	5	0.4478487146536949
2204	6	0.40795461991557147
2204	7	0.375829841916115
2204	8	0.5753267730675091
2204	9	0.8678282612579622
2204	10	-1.530429226098633
2204	11	-0.1083091106566556
2204	12	0.315651001821621
2204	13	0.4507423135452342
2204	14	0.6666607678693452
2204	15	0.7037855833031159
2204	16	0.5911660403913501
2204	17	0.6427848693982057
2204	18	0.8059867331593722
2204	19	0.7722265035281524
2204	20	-0.45058266434300265
2204	21	0.2551339547309297
2204	22	0.2922427258800866
2204	23	0.43318016693724104
2204	24	0.5360001848886355
2204	25	-0.07144498983275582
2205	1	0.3729513871440808
2205	2	0.21166208711367318
2205	3	0.4610474647338414
2205	4	0.28118407700556247
2205	5	0.15259175416911575
2205	6	0.09177274151343084
2205	7	0.19385857422073371
2205	8	0.2500542902000973
2205	9	0.4281812710924058
2205	10	0.38736600626266693
2205	11	0.23573706060663824
2205	12	0.21097177462297623
2205	13	0.18703085112147022
2205	14	0.5188095236831529
2205	15	-0.04439378992013587
2205	16	0.031468099657772086
2205	17	0.3619539235747311
2205	18	-0.1681061526483134
2205	19	-0.8649520126997317
2205	20	-0.19889469388555625
2205	21	-1.0552654534055463
2205	22	-1.6524539301954775
2205	23	-2.033055605201369
2205	24	-1.1323799005020092
2205	25	-1.8961495617735054
2206	1	0.033703220800678224
2206	2	-0.22971872865281995
2206	3	0.4108243729398815
2206	4	0.14087728912810604
2206	5	0.003205870559888524
2206	6	-0.08336786797752183
2206	7	-0.04453775496784118
2206	8	-0.06773153390385289
2206	9	0.4426711246295625
2206	10	-0.1318736512498191
2206	11	-0.043701798248889645
2206	12	0.20875430190830713
2206	13	0.1928766087731475
2206	14	0.01610719248256487
2206	15	-0.013632528705031177
2206	16	-0.5700527301935239
2206	17	-0.293798848244073
2206	18	0.17123485818525097
2206	19	-0.6566084114077242
2206	20	-0.4246575999764062
2206	21	-2.5685607490609144
2206	22	-1.364650233306321
2206	23	-1.944341961637285
2206	24	-1.1429930062924285
2206	25	-1.7811506624798241
2207	1	0.5379664254539449
2207	2	0.37613925951285765
2207	3	0.7276458691599395
2207	4	0.442667020425354
2207	5	0.3401718594631191
2207	6	0.3197378865860685
2207	7	0.2651826466614722
2207	8	0.23973146289451108
2207	9	-1.415140029132779
2207	10	-0.23484513825050612
2207	11	0.1373299331489335
2207	12	0.4848139428557232
2207	13	0.575150608471418
2207	14	0.7129052212134752
2207	15	0.7220005068146875
2207	16	0.5990085702406276
2207	17	0.6033930990546494
2207	18	0.6983455073965743
2207	19	-0.5613119667323261
2207	20	0.08182831114886113
2207	21	-0.46802803705729445
2207	22	0.11037750861834894
2207	23	-0.6464848786461843
2207	24	-1.6747780440918518
2207	25	-0.32821739396146654
2208	1	0.5449788969208242
2208	2	0.17484587808875318
2208	3	0.7179138865789945
2208	4	0.5404981154323352
2208	5	0.46557974457714124
2208	6	0.3420835861184557
2208	7	0.3548861238238956
2208	8	0.4720151929213259
2208	9	-0.01918458512417942
2208	10	0.17189003560761762
2208	11	0.23957138983399773
2208	12	0.3674351116609884
2208	13	0.6184410590177052
2208	14	0.950930563056879
2208	15	0.7604335952618229
2208	16	0.5072556670648609
2208	17	0.47156769959096323
2208	18	0.700179577604672
2208	19	-1.4446103954715497
2208	20	-0.11655211568446333
2208	21	-0.36146433894325014
2208	22	0.1984409407926293
2208	23	0.3282690716673997
2208	24	0.5015678604357703
2208	25	0.5548288629363469
2209	1	0.08667354629555746
2209	2	-1.3527892974515012
2209	3	-0.23572594828932836
2209	4	0.08787319929759008
2209	5	0.012340092820146253
2209	6	0.0964994950144984
2209	7	0.09368464302207968
2209	8	0.2057867967873932
2209	9	-2.2379208009471583
2209	10	-0.5594155541275398
2209	11	-0.14005769039290072
2209	12	0.2874452285287106
2209	13	0.38250246650427755
2209	14	0.5357657915902507
2209	15	0.6424416752756841
2209	16	0.5383819685784559
2209	17	0.4667277684157156
2209	18	0.46178472209157917
2209	19	0.2976940007030464
2209	20	0.19385672245361008
2209	21	0.3112888291523248
2209	22	0.2396690275422273
2209	23	0.24113776939802278
2209	24	0.3329009964260976
2209	25	0.38384294235442695
2210	1	0.12078821122346994
2210	2	0.07896736896457313
2210	3	0.062308790892736
2210	4	0.20763310933742218
2210	5	0.13826205269130298
2210	6	0.027186718826449408
2210	7	0.014287626629631234
2210	8	0.1357969280615663
2210	9	0.2866880077745293
2210	10	-1.4588615524489512
2210	11	-0.4927216007546278
2210	12	-2.338719517225009
2210	13	-0.771108112255888
2210	14	-0.4969779001670034
2210	15	-0.7370151340330153
2210	16	-0.16181996900247242
2210	17	0.02509102141023334
2210	18	0.3131733923712616
2210	19	0.1465608807177361
2210	20	0.21913861094888176
2210	21	0.17966309349936438
2210	22	0.1832474337607337
2210	23	0.09996823371415076
2210	24	0.1969263525097844
2210	25	0.12222144494478471
2211	1	-0.36548276612155917
2211	2	-0.10911977348333934
2211	3	-1.4083683906846822
2211	4	-0.6491023979734081
2211	5	-0.5059679396366903
2211	6	-0.4874891286162395
2211	7	-0.5469603486104049
2211	8	-0.16141605345836751
2211	9	-3.995509722116708E-4
2211	10	0.015958354678161868
2211	11	-0.3342327764515858
2211	12	-0.34750167158787093
2211	13	-0.36777057490301956
2211	14	-0.8920971259529225
2211	15	-0.5236219523686998
2211	16	-0.19307718723993617
2211	17	-0.3043270008131458
2211	18	-0.13761040757058396
2211	19	-0.17255011318256663
2211	20	-0.14619682689543367
2211	21	-0.11469873428433175
2211	22	-0.28108452803195405
2211	23	-0.5302496904163831
2211	24	-0.3799819959580475
2211	25	-0.2494139096594452
2212	1	-0.4597876175456541
2212	2	-0.37304542187991174
2212	3	-2.384166555696847
2212	4	-1.3921574980687126
2212	5	-0.6596860461923697
2212	6	-0.40868254411715554
2212	7	-0.38119763104427307
2212	8	-0.4064209687988338
2212	9	-0.980222922411734
2212	10	-0.3556263035302152
2212	11	-0.49323334627774074
2212	12	-0.10928861142420487
2212	13	-0.387638088810462
2212	14	-0.1334113763017868
2212	15	0.01863627144846447
2212	16	-1.1907001687678331
2212	17	-1.4360499571230037
2212	18	-0.31172085437527386
2212	19	-0.1462873020773401
2212	20	-0.247351950054842
2212	21	-0.08606220217198768
2212	22	-0.3695446076503046
2212	23	-0.12083013077376847
2212	24	-0.23356464325731707
2212	25	-0.052226063425621305
2213	1	-3.041027657386291
2213	2	-1.3448437286528225
2213	3	-2.313261675218758
2213	4	-1.0639513227699098
2213	5	-0.8342194127262346
2213	6	-0.6028508708103786
2213	7	-0.48236247168172497
2213	8	-0.3018143950936505
2213	9	-0.3686925439256825
2213	10	-0.2504161441676658
2213	11	-0.5161168124131947
2213	12	-0.26428960744014063
2213	13	-0.6394891844279869
2213	14	-0.35115838825397816
2213	15	-0.4769802624160775
2213	16	-1.6512340333096702
2213	17	-1.1806490607086602
2213	18	-0.40136677070993443
2213	19	-0.23503829809327215
2213	20	-0.2627234639990661
2213	21	-0.13227285954250334
2213	22	-0.18031701800887134
2213	23	-0.03522156503672136
2213	24	0.014271512121162289
2213	25	-0.036246979760278464
2214	1	-0.9412213373047429
2214	2	-0.8432253545447672
2214	3	-6.500358642598982
2214	4	-2.1534044318422865
2214	5	-1.2611494585977923
2214	6	-0.7489594335015113
2214	7	-0.6562782464013992
2214	8	-0.43886205054727156
2214	9	-0.22501173352812742
2214	10	-0.43145233173137854
2214	11	-0.892046985705754
2214	12	-0.5500118296623597
2214	13	-5.5794895666909845
2214	14	-4.065128062056221
2214	15	-2.3341109963610336
2214	16	-2.9411535032383114
2214	17	-3.499098984256065
2214	18	-1.341978518926041
2214	19	-0.8939039963603506
2214	20	-0.603377643407832
2214	21	-0.4758672785027471
2214	22	-0.5520154125042822
2214	23	-0.40877049470032745
2214	24	-0.27383437472898464
2214	25	-0.04779336100391163
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1647.0
3	202.0
4	205.0
5	499.0
6	68.0
7	82.0
8	135.0
9	320.0
10	869.0
11	2302.0
12	5130.0
13	9455.0
14	15524.0
15	21933.0
16	27714.0
17	31414.0
18	33119.0
19	33268.0
20	32573.0
21	32174.0
22	32081.0
23	32462.0
24	34057.0
25	36028.0
26	39916.0
27	44986.0
28	52856.0
29	64385.0
30	80408.0
31	100936.0
32	132758.0
33	178135.0
34	240653.0
35	332040.0
36	467271.0
37	706779.0
38	1358531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71696888634257	21.902688064351	12.663240146240963	22.717102903065463
2	14.527856388181323	22.421669792611258	41.11607823081982	21.93439558838761
3	17.726870859929136	29.608126714514395	29.708832757164004	22.956169668392466
4	11.458209001991774	18.291806366259024	39.73435530008513	30.515629331664073
5	11.509215308498463	40.48930651081189	35.52737923108293	12.474098949606717
6	28.300330408042328	40.436815173650224	18.475003367238575	12.787851051068868
7	24.0708626814263	34.903894390165306	24.498934366089564	16.52630856231883
8	23.359991809168946	38.62749516540024	21.742446207004118	16.27006681842669
9	24.534833343341163	17.412760244407917	22.53677637718684	35.515630035064085
10	14.161009216671443	31.658713780796997	34.08589499511248	20.094382007419078
11	30.616125557461572	27.739847519324417	23.24915263927539	18.394874283938627
12	19.594461085937564	29.45606478365022	32.56331940614987	18.38615472426234
13	26.059279764875082	25.717720918816738	26.96762343651546	21.25537587979272
14	19.395892925450337	24.99430044785442	29.08606455313956	26.523742073555677
15	22.675343124132336	31.67653378060103	24.848955047634632	20.799168047632
16	19.95756737477017	32.82421200141426	27.020453683179618	20.19776694063595
17	18.97764312003531	31.284106173350445	29.26654458158748	20.471706125026763
18	19.26309153823704	31.021755645049886	30.203641495010174	19.5115113217029
19	19.868873245333347	30.088697763715857	30.896367757794696	19.146061233156097
20	21.65934126134126	29.370956085604494	29.53128330431958	19.438419348734666
21	21.45280934060269	30.08446549244107	28.98956459209619	19.473160574860053
22	20.471006691757168	29.61006854433947	29.556361284074807	20.36256347982856
23	20.29832971816521	29.96531400288942	29.6399608332199	20.09639544572547
24	20.06832587023492	29.79644833714384	29.672347808777523	20.462877983843725
25	20.62598028473354	29.443136069023527	28.821017113295316	21.109866532947617
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5697.0
1	5697.0
2	5754.0
3	5811.0
4	5811.0
5	5811.0
6	9089.0
7	12367.0
8	12367.0
9	12367.0
10	18315.5
11	24264.0
12	24264.0
13	24264.0
14	38237.0
15	52210.0
16	52210.0
17	52210.0
18	79498.0
19	106786.0
20	106786.0
21	106786.0
22	149285.5
23	191785.0
24	191785.0
25	191785.0
26	245625.0
27	299465.0
28	299465.0
29	299465.0
30	350363.0
31	401261.0
32	401261.0
33	401261.0
34	437445.5
35	473630.0
36	473630.0
37	473630.0
38	492027.0
39	510424.0
40	510424.0
41	510424.0
42	500842.0
43	491260.0
44	491260.0
45	491260.0
46	497470.0
47	503680.0
48	503680.0
49	503680.0
50	457675.5
51	411671.0
52	411671.0
53	411671.0
54	350989.5
55	290308.0
56	290308.0
57	290308.0
58	241615.0
59	192922.0
60	192922.0
61	192922.0
62	155455.5
63	117989.0
64	117989.0
65	117989.0
66	87055.5
67	56122.0
68	56122.0
69	56122.0
70	39628.5
71	23135.0
72	23135.0
73	23135.0
74	15868.0
75	8601.0
76	8601.0
77	8601.0
78	5551.5
79	2502.0
80	2502.0
81	2502.0
82	1583.0
83	664.0
84	664.0
85	664.0
86	426.0
87	188.0
88	188.0
89	188.0
90	143.0
91	98.0
92	98.0
93	98.0
94	68.5
95	39.0
96	39.0
97	39.0
98	37.5
99	36.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10356414127468525
2	0.05527245951686802
3	0.0581173655214127
4	0.0530252228410092
5	0.050204223609611964
6	0.07009465886827727
7	0.06631738871098265
8	0.06289872015089955
9	0.06822993056277739
10	0.06887541343775812
11	0.06172728826667527
12	0.06218151695647653
13	0.06380717753050205
14	0.06603050743321344
15	0.06856462538684147
16	0.0631377878823739
17	0.06622176161839292
18	0.1461660110234131
19	0.07148125171082846
20	0.06488298232213659
21	0.06282699983145724
22	0.06806258315074536
23	0.06794304928500818
24	0.07743403822453958
25	0.07188766685433483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4182915.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.83398056807174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62024462575089	62.222769692478096
2	9.815322209405029	14.101473297195275
3	2.0174380715702154	4.347618216913889
4	0.5886475377282453	1.6913958314645618
5	0.25250284899987196	0.9069142374219775
6	0.1448995570164482	0.6245227177825045
7	0.09694401382018282	0.4874712083464923
8	0.06523757495584195	0.37490197533488595
9	0.049581493986090755	0.3205472467979528
>10	0.2927660345793638	3.8984248751238466
>50	0.02870183997643624	1.4415028696573855
>100	0.022832497935913264	3.235472734952453
>500	0.002424013894557905	1.1663093828560585
>1k	0.0021546786901909206	3.038919264825985
>5k	2.0200112720538822E-4	0.9951331792821425
>10k+	1.0100056360269411E-4	1.146623269566563
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	17463	0.4174839794736446	No Hit
CCCATGTACTCTGCGTTGATACCAC	16656	0.3981912135436651	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13293	0.3177927354488437	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8806	0.21052304433630617	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7911	0.18912648236935248	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7728	0.184751542883372	No Hit
CATGTACTCTGCGTTGATACCACTG	6350	0.1518080094862076	No Hit
GCGTTGATACCACTGCTTCCCATGT	5215	0.12467382196386971	No Hit
GTATACGGTGCTACATTCTAAAGCA	5138	0.12283300043151725	No Hit
ACAGTATACCATGTGACATGAGGAC	4955	0.11845806094553679	No Hit
GTATCAACGCAGAGTACATGGGAAG	4859	0.1161630107233831	No Hit
ATAGTATACGGTGCTACATTCTAAA	4419	0.10564403053851203	No Hit
GGTATCAACGCAGAGTACATGGGAA	4407	0.1053571492607428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3906773147434266E-5	0.0
4	0.0	0.0	0.0	2.3906773147434266E-5	0.0
5	0.0	0.0	0.0	2.3906773147434266E-5	0.0
6	0.0	0.0	0.0	2.3906773147434266E-5	0.0
7	0.0	0.0	0.0	2.3906773147434266E-5	0.0
8	0.0	0.0	0.0	2.3906773147434266E-5	0.0
9	0.0	0.0	0.0	9.562709258973706E-5	0.0
10	0.0	0.0	0.0	1.1953386573717133E-4	0.0
11	0.0	0.0	0.0	1.1953386573717133E-4	0.0
12	0.0	0.0	0.0	1.1953386573717133E-4	0.0
13	0.0	0.0	0.0	1.1953386573717133E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	55	1.9661197E-4	13.814266	8
GTCGCGT	105	1.366061E-9	13.576678	15
CGCGTCA	110	2.732122E-9	12.960487	17
TCGCGTC	115	5.298716E-9	12.39684	16
GCGCTAT	330	0.0	11.798416	11
ATCGCCG	90	7.4845066E-6	11.6089325	19
GTGCGCT	355	0.0	11.503782	9
TTGTGCG	375	0.0	11.143641	7
ACTCGCC	60	0.0058631417	11.087223	12
TGCGCTA	370	0.0	10.780728	10
TGTGCGC	405	0.0	10.552565	8
CTGCGTG	125	2.5822155E-6	9.8772	9
CGTCGCG	145	1.6105878E-7	9.83127	14
CGCCAGT	530	0.0	9.680209	1
CCGTCCC	100	2.7651968E-4	9.497309	9
GTATTAG	585	0.0	9.41974	1
TCGTCGC	155	4.241938E-7	9.197104	13
GGCGCCC	115	1.11491856E-4	9.084382	8
TAAGACT	525	0.0	9.04538	4
GCGTGCC	105	4.5130937E-4	9.044082	11
>>END_MODULE