FastQCFastQC Report
Wed 25 May 2016
SRR1294636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182915
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT372810.8912684097094968No Hit
GGTATCAACGCAGAGTACTTTTTTT273210.6531569491610515No Hit
TATCAACGCAGAGTACTTTTTTTTT238010.569005107682083No Hit
CCCATGTACTCTGCGTTGATACCAC185280.442944692875662No Hit
GTACATGGGAAGCAGTGGTATCAAC173350.414423912510773No Hit
ACGCAGAGTACTTTTTTTTTTTTTT146710.3507362688460081No Hit
GTACTTTTTTTTTTTTTTTTTTTTT88620.21186182363256245No Hit
GAGTACATGGGAAGCAGTGGTATCA84580.20220348728099904No Hit
GAGTACTTTTTTTTTTTTTTTTTTT77810.18601860186018604No Hit
CATGTACTCTGCGTTGATACCACTG71530.17100514832359728No Hit
GCGTTGATACCACTGCTTCCCATGT54200.12957471045909372No Hit
ACAGTATACCATGTGACATGAGGAC53940.1289531343572604No Hit
ACGCAGAGTACATGGGAAGCAGTGG50100.11977293346864568No Hit
GTATACGGTGCTACATTCTAAAGCA50020.1195816792834662No Hit
GTATCAACGCAGAGTACATGGGAAG49700.11881666254274831No Hit
GGTATCAACGCAGAGTACATGGGAA49350.1179799254825881No Hit
GCAGAGTACTTTTTTTTTTTTTTTT47590.11377233340863968No Hit
ATAGTATACGGTGCTACATTCTAAA47010.1123857405660885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG604.018414E-412.6948988
GTCGCGT751.5090995E-512.64269615
GCGCTAT3300.012.07741211
TCGCGTC802.9245126E-511.85281116
TGTGCGC3550.011.8008918
ATCGCCG658.1553427E-411.6704619
GTGCGCT3600.011.6362919
TGCGCTA3450.011.59029810
CGCGCCG907.6259967E-611.58941517
TCGTCGA1407.039489E-1011.51415918
CGTCGAA1407.039489E-1011.51415919
CGACTCG1501.8189894E-1011.37842714
TTGTGCG3350.011.3685657
TCGGGAA2000.010.90432513
CGCCCTA2800.010.83685616
TGTCCCG3200.010.67556911
CGCAGGA4950.010.5355815
TCCGCGC1002.4517469E-510.43022315
GTCCCGC2950.010.2854612
TGCGCAC856.467801E-410.08063710
Produced by FastQC (version 0.11.5)