##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4182915 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.334606369003435 31.0 31.0 34.0 28.0 34.0 2 31.44850469110656 31.0 31.0 34.0 28.0 34.0 3 31.546594898533677 31.0 31.0 34.0 28.0 34.0 4 35.125313328145566 37.0 35.0 37.0 32.0 37.0 5 35.27596233726958 37.0 35.0 37.0 32.0 37.0 6 34.87118146077556 37.0 35.0 37.0 32.0 37.0 7 34.72723782338393 37.0 35.0 37.0 32.0 37.0 8 34.713612636164015 37.0 35.0 37.0 32.0 37.0 9 36.16195978163553 39.0 35.0 39.0 31.0 39.0 10 35.93582776604354 39.0 35.0 39.0 30.0 39.0 11 36.120858779105 39.0 35.0 39.0 30.0 39.0 12 36.07090509847797 39.0 35.0 39.0 30.0 39.0 13 35.927366441823466 39.0 35.0 39.0 30.0 39.0 14 36.96367031125423 40.0 36.0 41.0 30.0 41.0 15 37.01828127035811 40.0 36.0 41.0 30.0 41.0 16 37.054608329358835 40.0 36.0 41.0 31.0 41.0 17 37.12848145372306 40.0 36.0 41.0 31.0 41.0 18 37.143839881996165 40.0 36.0 41.0 31.0 41.0 19 37.178105459948384 40.0 37.0 41.0 30.0 41.0 20 37.25191068907687 40.0 37.0 41.0 31.0 41.0 21 37.13189438465759 40.0 37.0 41.0 30.0 41.0 22 37.10342237410992 40.0 36.0 41.0 30.0 41.0 23 37.1156413649333 40.0 36.0 41.0 30.0 41.0 24 37.030622902927746 40.0 36.0 41.0 30.0 41.0 25 35.968184866295395 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4583444435679951 1101 2 0.4495970980994741 1101 3 0.39017967253003505 1101 4 0.31493295726657067 1101 5 0.3407952906204912 1101 6 0.36623853375500204 1101 7 0.4354357571730958 1101 8 0.45633921969302804 1101 9 0.609159529640138 1101 10 0.6894633486036597 1101 11 0.7324842691963624 1101 12 0.6966308014107838 1101 13 0.6673052819171303 1101 14 0.8320064514652259 1101 15 0.9066604257726496 1101 16 0.8754052798384251 1101 17 0.9327954561590133 1101 18 0.8854046371162809 1101 19 0.9294094763281464 1101 20 0.9104970376100923 1101 21 1.2276268415072593 1101 22 1.1166865666670631 1101 23 1.025633815886124 1101 24 1.1850209381026744 1101 25 1.1523583881205468 1102 1 0.3250791289514332 1102 2 0.32697044002273046 1102 3 0.2702195645592944 1102 4 0.24948569938558762 1102 5 0.2637038315868807 1102 6 0.2838572026590356 1102 7 0.35921814956031284 1102 8 0.39531090718339357 1102 9 0.483381491243577 1102 10 0.5959763844619701 1102 11 0.5116635192988497 1102 12 0.6093245145433173 1102 13 0.6255278143249825 1102 14 0.7296386094803466 1102 15 0.7302648917386776 1102 16 0.6865264203802042 1102 17 0.6266912236089226 1102 18 0.6276766250985304 1102 19 0.7789344568152217 1102 20 0.7292523175911043 1102 21 1.0486977040149057 1102 22 0.9125738983605132 1102 23 0.9458647666949034 1102 24 1.0102679773359995 1102 25 0.8459419486962787 1103 1 0.3001251115103116 1103 2 0.29901031180216364 1103 3 0.29286795660743437 1103 4 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0.17849221770601176 2207 4 0.22471142668755562 2207 5 0.15656912830513647 2207 6 0.356930759463026 2207 7 0.25025952479501967 2207 8 0.3089664551752236 2207 9 0.3621106858066696 2207 10 0.6078672573739468 2207 11 0.4219613222447123 2207 12 0.38925344878130375 2207 13 0.5162249320472867 2207 14 0.5017398370478787 2207 15 0.5148638190197019 2207 16 0.5224824044913987 2207 17 0.5971250239814552 2207 18 0.5780055473675461 2207 19 0.6532430617569105 2207 20 0.599440305233145 2207 21 0.904453357483284 2207 22 0.7803037586975918 2207 23 0.6899234445725 2207 24 0.8826911702084743 2207 25 0.7869542243715912 2208 1 0.16918439559821508 2208 2 0.14459770272058847 2208 3 0.1379619448961371 2208 4 0.11312767228292842 2208 5 0.12016130967605676 2208 6 0.19960508851915648 2208 7 0.23780046843795333 2208 8 0.17438456234894772 2208 9 0.3024756604315826 2208 10 0.31245122785463053 2208 11 6.322605688495742E-4 2208 12 -0.24196959711546384 2208 13 -0.03308085435875796 2208 14 -0.34757002156081285 2208 15 0.12581208819506884 2208 16 0.22377564740521194 2208 17 0.17317196789032607 2208 18 0.19779972107053823 2208 19 0.4355493412596019 2208 20 0.35438758821521077 2208 21 0.6053594641251294 2208 22 0.5398677282918101 2208 23 0.5236363792178054 2208 24 0.5935074113715189 2208 25 0.5110747394082722 2209 1 -0.04421079692281893 2209 2 0.004813993308498965 2209 3 0.07426982076053434 2209 4 -0.5997239153890774 2209 5 -0.09903329218608548 2209 6 0.09658676258010956 2209 7 0.12519009557679084 2209 8 0.0810668164613304 2209 9 0.2052924335264592 2209 10 0.2876803159045309 2209 11 0.2857247587501064 2209 12 0.3036674097590577 2209 13 0.31726033026501455 2209 14 0.32574959610202825 2209 15 -0.3060620063032857 2209 16 -0.3353551019501637 2209 17 0.15557902110177935 2209 18 -0.16638591527557622 2209 19 0.20072572016347578 2209 20 0.20156805276020862 2209 21 0.49865012783408247 2209 22 0.5347932727419433 2209 23 0.4522500600604644 2209 24 0.582527886205753 2209 25 0.5899878545637733 2210 1 -0.16531593838749714 2210 2 -0.10013682412432345 2210 3 -0.07830955949506091 2210 4 -0.0036598692703506686 2210 5 -0.05317662408300805 2210 6 -0.5111121977754749 2210 7 -0.23626755548426104 2210 8 -0.3044743533800869 2210 9 -0.021535231791098397 2210 10 0.17435382228681817 2210 11 0.08506249979098612 2210 12 0.06439130368428181 2210 13 0.14184611063382135 2210 14 0.16465286451757066 2210 15 0.007312220099926492 2210 16 0.16824467510393504 2210 17 -0.9107472212131569 2210 18 -1.4864884280248418 2210 19 -0.3105738416372148 2210 20 -0.15346862963703956 2210 21 -0.3427717916091524 2210 22 -0.3179755427408395 2210 23 -0.1891182848861348 2210 24 0.2869513336113272 2210 25 0.29016268551311697 2211 1 -0.22182051549654602 2211 2 -0.22498418988692137 2211 3 -0.16171275138543706 2211 4 -0.06973409209372505 2211 5 -0.15371867814744888 2211 6 -0.032203046274993596 2211 7 -0.16568022959001638 2211 8 -0.15646647661173319 2211 9 -0.1355948709749839 2211 10 -0.09303053197631783 2211 11 -0.38671607644792516 2211 12 -0.14657611458823538 2211 13 -0.13867877661940042 2211 14 -0.19071984237160677 2211 15 -0.23725686199642837 2211 16 -0.19729652334599734 2211 17 -0.3906006400725346 2211 18 -0.4165417802055842 2211 19 -0.2010861123352612 2211 20 -0.29079825745273524 2211 21 -0.03134287828005 2211 22 -0.0412963258435326 2211 23 -0.7372993867068516 2211 24 -0.21261001113870037 2211 25 -0.7505595908413483 2212 1 -0.22954419192435438 2212 2 -0.31929298919340354 2212 3 -0.23532393779034777 2212 4 -0.08605173368869146 2212 5 -0.08674141649790101 2212 6 -0.14608425149904747 2212 7 -0.17656221192673627 2212 8 -0.38627029015115966 2212 9 -0.12869199105983142 2212 10 -0.5263956318973726 2212 11 -0.18023462768339016 2212 12 -0.31783980542081736 2212 13 -0.3323034396729341 2212 14 -0.30178219906801473 2212 15 -0.17311532947507402 2212 16 -0.3637347977057317 2212 17 -0.555118988135753 2212 18 -0.4526695628424946 2212 19 -0.5090444744886398 2212 20 -0.691717784302206 2212 21 -0.09374859800835367 2212 22 -0.4804731926685406 2212 23 -0.29876502639569225 2212 24 -0.5115237747454344 2212 25 -0.19550717264389306 2213 1 -0.1958390126414855 2213 2 -0.2153358178786604 2213 3 -0.17286577045966922 2213 4 -0.03729227153729653 2213 5 -0.012302173470011724 2213 6 -0.2794871399452603 2213 7 -0.28921310834107317 2213 8 -0.3015143140555452 2213 9 -0.2775505567968821 2213 10 -0.21063865978581475 2213 11 -0.06031530497422466 2213 12 0.06304117067480064 2213 13 0.06922345275734898 2213 14 -0.6710227369166191 2213 15 -0.1687796721045558 2213 16 -0.32556049491688555 2213 17 -0.21607615945049474 2213 18 -0.14746338754369503 2213 19 -0.22358829918983503 2213 20 -0.3347875054177436 2213 21 0.006804688844233908 2213 22 -0.44881532414264314 2213 23 -0.48712907022039786 2213 24 -0.8270033564187429 2213 25 -0.4564215152733766 2214 1 -0.3125986966374086 2214 2 -0.18078255590108938 2214 3 -0.11815068381338989 2214 4 -0.09775085971262598 2214 5 -0.11888907459131559 2214 6 -0.11907518785555737 2214 7 -0.15113423474270604 2214 8 -0.08099825900967517 2214 9 -0.17637853844825457 2214 10 -0.16103876172261522 2214 11 -0.0662197392249908 2214 12 -0.15403693839757437 2214 13 -0.289905752135617 2214 14 -0.24540665638654957 2214 15 -0.09189205742566031 2214 16 -0.4298640117364556 2214 17 -0.4976877802455988 2214 18 -0.39348453942952233 2214 19 -0.5456163318096117 2214 20 -0.4739816950201927 2214 21 -0.4498965089131559 2214 22 -0.6112693251620129 2214 23 -0.9636791466729946 2214 24 -0.5893857468366832 2214 25 -0.8113404755180227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 2.0 8 15.0 9 66.0 10 140.0 11 314.0 12 770.0 13 1856.0 14 3708.0 15 6751.0 16 9807.0 17 13170.0 18 15625.0 19 17485.0 20 18074.0 21 19692.0 22 20894.0 23 22980.0 24 25551.0 25 28998.0 26 32975.0 27 37138.0 28 44132.0 29 55347.0 30 70466.0 31 93205.0 32 124169.0 33 169822.0 34 232245.0 35 325589.0 36 468778.0 37 741799.0 38 1581350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93154402822891 21.929955122998972 11.86005286459991 24.278447984172207 2 14.4205416557592 22.459743982366366 40.132084921639574 22.987629440234862 3 17.33618046063136 29.246596493915938 30.255164208545693 23.162058836907004 4 11.348980800984696 18.124776145382484 39.754404287297106 30.77183876633571 5 11.646035880971931 39.64221851208698 35.86550063630786 12.846244970633233 6 28.65097293606147 40.356762350348795 17.852869840990515 13.139394872599222 7 23.976016403536565 35.00524267549812 24.345550301588347 16.673190619376975 8 24.003115160511587 37.8481242085369 21.437435678678415 16.711324952273102 9 24.831678065039455 15.928372301846538 22.936680160472132 36.30326947264187 10 14.381035204102085 30.93344170066948 34.172580103536255 20.512942991692185 11 31.089556044952893 26.58256857689516 23.328009821544338 18.999865556607606 12 20.052084018172266 28.74413349535975 32.940786644152546 18.262995842315433 13 26.916146714024414 24.529269434960757 27.371523146668363 21.183060704346467 14 19.816638253222244 23.972627465437082 29.87750781220303 26.333226469137642 15 23.048536169032673 30.513428611046027 25.73550154678856 20.702533673132738 16 19.829477523895452 32.024985482591184 27.760871680450137 20.38466531306323 17 18.782456440653572 30.570530496204324 29.93798103427419 20.709032028867913 18 18.967611974838533 29.89855851341556 31.482768908872877 19.651060602873027 19 19.534384789868074 29.107178375938584 32.51883384143426 18.839602992759083 20 21.22144631369071 28.13849688374839 31.426781833699508 19.21327496886139 21 21.022561826634405 29.13350192426031 30.620028276992116 19.223907972113167 22 19.953552633057434 28.75024594774934 31.262152448635472 20.034048970557752 23 19.86070472897715 28.97442523906187 31.410407053648974 19.754462978312006 24 19.573383633184037 28.94703813010783 31.434537876098368 20.045040360609768 25 20.132527245855563 28.582770836451232 30.52367794306162 20.76102397463158 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2848.0 1 2848.0 2 3402.5 3 3957.0 4 3957.0 5 3957.0 6 9592.5 7 15228.0 8 15228.0 9 15228.0 10 19948.0 11 24668.0 12 24668.0 13 24668.0 14 33959.0 15 43250.0 16 43250.0 17 43250.0 18 70432.0 19 97614.0 20 97614.0 21 97614.0 22 142568.0 23 187522.0 24 187522.0 25 187522.0 26 245831.5 27 304141.0 28 304141.0 29 304141.0 30 357374.0 31 410607.0 32 410607.0 33 410607.0 34 445642.0 35 480677.0 36 480677.0 37 480677.0 38 492560.5 39 504444.0 40 504444.0 41 504444.0 42 495339.5 43 486235.0 44 486235.0 45 486235.0 46 495771.0 47 505307.0 48 505307.0 49 505307.0 50 460614.0 51 415921.0 52 415921.0 53 415921.0 54 354736.0 55 293551.0 56 293551.0 57 293551.0 58 244513.5 59 195476.0 60 195476.0 61 195476.0 62 157646.5 63 119817.0 64 119817.0 65 119817.0 66 88302.0 67 56787.0 68 56787.0 69 56787.0 70 40021.5 71 23256.0 72 23256.0 73 23256.0 74 15766.0 75 8276.0 76 8276.0 77 8276.0 78 5345.5 79 2415.0 80 2415.0 81 2415.0 82 1529.0 83 643.0 84 643.0 85 643.0 86 398.0 87 153.0 88 153.0 89 153.0 90 126.0 91 99.0 92 99.0 93 99.0 94 59.5 95 20.0 96 20.0 97 20.0 98 11.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0941209658814487 2 0.0 3 0.13399746349136904 4 0.012264174624633778 5 0.023356917365043278 6 0.012503242356108122 7 0.021277028101216497 8 0.018814630467030767 9 1.9125418517947413E-4 10 0.4025422462564982 11 0.0649547026415789 12 0.0013865928425511873 13 4.781354629486853E-5 14 8.606438333076335E-4 15 0.0013148725231088847 16 1.434406388846056E-4 17 2.3906773147434266E-5 18 2.6297450462177695E-4 19 4.54228689801251E-4 20 1.1953386573717132E-4 21 2.1516095832690838E-4 22 0.0027731856851023746 23 4.781354629486853E-5 24 0.0 25 0.0018408215323524385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4182915.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.71905703591248 #Duplication Level Percentage of deduplicated Percentage of total 1 85.78489905388598 58.950573709039844 2 10.423257668583368 14.32552876454785 3 2.1408411435968215 4.413497539549742 4 0.6374025361600828 1.7520680494887995 5 0.27373983431793497 0.9405571643747702 6 0.1546443037345364 0.6376206437167548 7 0.09263639702160968 0.4456120095370599 8 0.07631819805046572 0.4195611683766414 9 0.05417777621638953 0.33507411245036767 >10 0.29896521313316327 3.8431271361142145 >50 0.032305795696189916 1.5367912582452437 >100 0.02513565485054411 3.3572455922930367 >500 0.0030134108433938238 1.373269193604521 >1k 0.002172458715669535 3.0459113552297694 >5k 2.8031725363477457E-4 1.278109162554013 >10k+ 2.102379402260809E-4 3.345453140877296 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 37281 0.8912684097094968 No Hit GGTATCAACGCAGAGTACTTTTTTT 27321 0.6531569491610515 No Hit TATCAACGCAGAGTACTTTTTTTTT 23801 0.569005107682083 No Hit CCCATGTACTCTGCGTTGATACCAC 18528 0.442944692875662 No Hit GTACATGGGAAGCAGTGGTATCAAC 17335 0.414423912510773 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 14671 0.3507362688460081 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 8862 0.21186182363256245 No Hit GAGTACATGGGAAGCAGTGGTATCA 8458 0.20220348728099904 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7781 0.18601860186018604 No Hit CATGTACTCTGCGTTGATACCACTG 7153 0.17100514832359728 No Hit GCGTTGATACCACTGCTTCCCATGT 5420 0.12957471045909372 No Hit ACAGTATACCATGTGACATGAGGAC 5394 0.1289531343572604 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5010 0.11977293346864568 No Hit GTATACGGTGCTACATTCTAAAGCA 5002 0.1195816792834662 No Hit GTATCAACGCAGAGTACATGGGAAG 4970 0.11881666254274831 No Hit GGTATCAACGCAGAGTACATGGGAA 4935 0.1179799254825881 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4759 0.11377233340863968 No Hit ATAGTATACGGTGCTACATTCTAAA 4701 0.1123857405660885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.3906773147434266E-5 0.0 6 0.0 0.0 0.0 4.781354629486853E-5 0.0 7 0.0 0.0 0.0 4.781354629486853E-5 0.0 8 0.0 0.0 0.0 4.781354629486853E-5 0.0 9 0.0 0.0 0.0 4.781354629486853E-5 0.0 10 0.0 0.0 0.0 7.17203194423028E-5 0.0 11 0.0 2.3906773147434266E-5 0.0 7.17203194423028E-5 0.0 12 0.0 2.3906773147434266E-5 0.0 7.17203194423028E-5 0.0 13 0.0 2.3906773147434266E-5 0.0 7.17203194423028E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGTCG 60 4.018414E-4 12.694898 8 GTCGCGT 75 1.5090995E-5 12.642696 15 GCGCTAT 330 0.0 12.077412 11 TCGCGTC 80 2.9245126E-5 11.852811 16 TGTGCGC 355 0.0 11.800891 8 ATCGCCG 65 8.1553427E-4 11.67046 19 GTGCGCT 360 0.0 11.636291 9 TGCGCTA 345 0.0 11.590298 10 CGCGCCG 90 7.6259967E-6 11.589415 17 TCGTCGA 140 7.039489E-10 11.514159 18 CGTCGAA 140 7.039489E-10 11.514159 19 CGACTCG 150 1.8189894E-10 11.378427 14 TTGTGCG 335 0.0 11.368565 7 TCGGGAA 200 0.0 10.904325 13 CGCCCTA 280 0.0 10.836856 16 TGTCCCG 320 0.0 10.675569 11 CGCAGGA 495 0.0 10.53558 15 TCCGCGC 100 2.4517469E-5 10.430223 15 GTCCCGC 295 0.0 10.28546 12 TGCGCAC 85 6.467801E-4 10.080637 10 >>END_MODULE