FastQCFastQC Report
Wed 25 May 2016
SRR1294635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772056
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC205500.7413270150386573No Hit
CCCATGTACTCTGCGTTGATACCAC182990.6601237493037658No Hit
GAGTACATGGGAAGCAGTGGTATCA91380.3296470201179197No Hit
GTATCAACGCAGAGTACTTTTTTTT74610.26915040677388913No Hit
CATGTACTCTGCGTTGATACCACTG66470.23978592063075205No Hit
GCGTTGATACCACTGCTTCCCATGT57960.2090866851174724No Hit
GTATCAACGCAGAGTACATGGGAAG57430.20717474683051135No Hit
ACGCAGAGTACATGGGAAGCAGTGG49290.1778102606873743No Hit
GGTATCAACGCAGAGTACTTTTTTT47440.1711365138366613No Hit
TATCAACGCAGAGTACTTTTTTTTT45420.16384950376182877No Hit
ATTCCATTCCATTCCATTCCATTCC43110.15551633877526283No Hit
GCTTCCCATGTACTCTGCGTTGATA42620.15374869771750643No Hit
TATCAACGCAGAGTACATGGGAAGC41930.15125957051372702No Hit
GGTATCAACGCAGAGTACATGGGAA41000.14790465993471993No Hit
ACTCTGCGTTGATACCACTGCTTCC36890.1330781196339468No Hit
GTGGTATCAACGCAGAGTACATGGG31420.1133454735402171No Hit
GAATGGAATGGAATGGAATGGAATG30950.11164998109706298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT604.1049303E-412.6625986
CGCCCTA1251.4006218E-912.16619916
ACTCGAC1302.6338967E-911.68791911
GTCCTAG2000.011.400251
TAGGACT2550.011.1728814
TGTCCCG1451.2259989E-911.1337511
CCGTGCA600.00589169511.0797739
TAGGACG700.001496043510.8536564
CTCGACT1451.4346369E-810.48753312
ACACCGT1002.4087543E-510.4466456
TACACCG750.002655757410.1300785
CCCGCCC1606.1281753E-910.09871314
CTAGGAC1901.0004442E-109.9969693
TATACTG7000.09.903965
GTCCCGC1651.0155418E-89.79233712
GTCTAGG2401.8189894E-129.5002081
GTCTTAC1804.1836756E-99.5002081
CCTAGAC2303.6379788E-129.497123
GTTACAC1502.661891E-79.497123
TATTCCG1701.6749254E-89.4969495
Produced by FastQC (version 0.11.5)