##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294635_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2772056 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.59183688929805 31.0 30.0 33.0 25.0 34.0 2 29.788865376457043 31.0 30.0 34.0 25.0 34.0 3 29.752264745012365 31.0 30.0 34.0 25.0 34.0 4 33.39973254508567 35.0 33.0 37.0 27.0 37.0 5 33.76608733734095 35.0 35.0 37.0 28.0 37.0 6 33.82626469306536 36.0 35.0 37.0 28.0 37.0 7 33.777699296118115 36.0 35.0 37.0 28.0 37.0 8 33.57584334515609 36.0 35.0 37.0 27.0 37.0 9 34.66652405290514 38.0 34.0 39.0 26.0 39.0 10 34.80699668404967 38.0 34.0 39.0 27.0 39.0 11 35.00372972263187 38.0 35.0 39.0 27.0 39.0 12 34.79329710510899 38.0 34.0 39.0 27.0 39.0 13 34.62005493395516 38.0 34.0 39.0 26.0 39.0 14 35.46079696802662 39.0 34.0 41.0 25.0 41.0 15 35.44391274923739 39.0 34.0 41.0 25.0 41.0 16 35.54420762062527 39.0 34.0 41.0 26.0 41.0 17 35.53414324963132 39.0 34.0 41.0 26.0 41.0 18 35.43008474576271 39.0 34.0 41.0 25.0 41.0 19 35.693931147134116 39.0 34.0 41.0 26.0 41.0 20 35.81726920379675 39.0 34.0 41.0 27.0 41.0 21 35.86657340255753 39.0 34.0 41.0 27.0 41.0 22 35.87428212128471 39.0 34.0 41.0 27.0 41.0 23 35.85814283694125 39.0 34.0 41.0 27.0 41.0 24 35.88257993345012 39.0 34.0 41.0 27.0 41.0 25 34.53099865226388 38.0 32.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0496487199296247 1101 2 1.0847319854394044 1101 3 1.3711857167972354 1101 4 1.1158714057357955 1101 5 0.8622996730679233 1101 6 0.848709453302888 1101 7 0.8596808228352515 1101 8 1.0217128481347402 1101 9 1.3228513377504711 1101 10 1.2204616768970737 1101 11 0.9899119683111977 1101 12 1.282099842492542 1101 13 1.2764464112960567 1101 14 1.3917191788154142 1101 15 1.579592869190833 1101 16 1.4208403196258672 1101 17 1.3895259314291337 1101 18 1.4971213449725482 1101 19 1.347392662261349 1101 20 1.2663648312275768 1101 21 1.1042943472718179 1101 22 1.2037493886085642 1101 23 1.2025418625877577 1101 24 1.2271883305705344 1101 25 1.2107872271115738 1102 1 0.8403697903571157 1102 2 0.832057074559831 1102 3 1.0241039519650919 1102 4 0.9910640383293767 1102 5 0.72819332751898 1102 6 0.6811999817208161 1102 7 0.6640020719553092 1102 8 0.7404564023135265 1102 9 1.064848602463698 1102 10 0.964210768489508 1102 11 0.7204292459936852 1102 12 1.0523254730178238 1102 13 0.9511466978085394 1102 14 1.1800397168204526 1102 15 1.2683319314791959 1102 16 1.1331702005691398 1102 17 1.197090015980514 1102 18 1.291380752630765 1102 19 0.9216227523473464 1102 20 0.1378691430720025 1102 21 0.48141961424715163 1102 22 0.6049526004919414 1102 23 0.7336448201983572 1102 24 0.8003174800179096 1102 25 0.7494704272025672 1103 1 0.7459946614751729 1103 2 0.7824150422320209 1103 3 1.115765511373855 1103 4 0.9071209553401687 1103 5 0.7333838999256272 1103 6 0.5576286590871362 1103 7 0.47826945244445085 1103 8 0.7778423876685565 1103 9 0.8303587170295401 1103 10 0.8534382890430479 1103 11 0.7698622546165481 1103 12 1.0644085798001939 1103 13 0.9596802774976112 1103 14 0.8106422484014146 1103 15 1.0200765625472172 1103 16 0.9831093250733147 1103 17 1.016492231508309 1103 18 1.1265617882880008 1103 19 0.8883015817652051 1103 20 0.07326263928604959 1103 21 0.4629455408011509 1103 22 -0.1742874285559779 1103 23 0.47568041336126043 1103 24 0.6418083630309042 1103 25 0.6197368497635765 1104 1 0.2937086232413506 1104 2 0.36919667167683556 1104 3 0.6084309947661275 1104 4 0.43238633187439746 1104 5 0.2835787603343931 1104 6 0.19972832059321632 1104 7 0.19830729043256667 1104 8 0.33518589169307944 1104 9 0.7030580828454802 1104 10 0.6316453585753763 1104 11 0.38762432177195905 1104 12 0.4880160876101627 1104 13 0.4532633582519594 1104 14 0.774926763316266 1104 15 0.7222346023489479 1104 16 0.6144872598502715 1104 17 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0.4168519962844641 2207 7 0.29732756658318493 2207 8 0.3110698342981735 2207 9 -1.2104296372572563 2207 10 -0.2452651999943214 2207 11 0.11951101258292596 2207 12 0.4450459895643206 2207 13 0.596723128030689 2207 14 0.7902381210587706 2207 15 0.7608577029395533 2207 16 0.8016537601756255 2207 17 0.8113091727175572 2207 18 0.8075297934223045 2207 19 -0.40023553994065963 2207 20 0.27701038183459303 2207 21 -0.4037118390128782 2207 22 0.2688223072164817 2207 23 -0.5605781127157243 2207 24 -1.724291805624972 2207 25 -0.30041155745828263 2208 1 0.5028007057389168 2208 2 0.1880302966174483 2208 3 0.6410706541126139 2208 4 0.48242059505388823 2208 5 0.4186067562545972 2208 6 0.3076418016057758 2208 7 0.36877200811402133 2208 8 0.4942031724646725 2208 9 -0.2055197114359899 2208 10 0.056525486093427446 2208 11 0.2638242384247036 2208 12 0.2837280174557719 2208 13 0.48474128014877493 2208 14 0.7308453100255861 2208 15 0.7093720458584798 2208 16 0.40714579394399664 2208 17 0.38997380123002046 2208 18 0.589320325260239 2208 19 -1.4453907538157367 2208 20 -0.22451200212712763 2208 21 -0.44948682863957856 2208 22 0.1654492031395094 2208 23 0.31755629748697345 2208 24 0.4154264725152075 2208 25 0.33426512721962354 2209 1 0.14759155454722972 2209 2 -1.4204069758235036 2209 3 -0.3287070122313587 2209 4 -0.01745093105528639 2209 5 0.018793593600705094 2209 6 0.11234870361444393 2209 7 0.09399552962345581 2209 8 0.17949980693160938 2209 9 -2.2831734417212957 2209 10 -0.5615280284436537 2209 11 -0.10582131695286279 2209 12 0.1736614744496805 2209 13 0.3908994372193604 2209 14 0.4882217249060261 2209 15 0.6561862385012205 2209 16 0.5062958480745152 2209 17 0.28284366279672923 2209 18 0.5322139361566016 2209 19 0.2570889114494932 2209 20 0.2495373707868751 2209 21 0.313364731472376 2209 22 0.20314017088793435 2209 23 0.29115540044484334 2209 24 0.36231962839020326 2209 25 0.36880087773790393 2210 1 0.20993053469442913 2210 2 0.2154995887059883 2210 3 0.1528289155915168 2210 4 0.27666647120919663 2210 5 0.1886651383821274 2210 6 0.07473460471700122 2210 7 0.2045129328589539 2210 8 0.2033587485987738 2210 9 0.29343065326779794 2210 10 -1.5891028462131374 2210 11 -0.43196946718176576 2210 12 -2.349374703236826 2210 13 -0.6702549015124788 2210 14 -0.4832234231793535 2210 15 -0.7289044111892835 2210 16 0.053820048086095085 2210 17 0.1410560264164502 2210 18 0.3891560007410604 2210 19 0.24687311320197125 2210 20 0.28449885780315753 2210 21 0.23394381859797164 2210 22 0.20514740288795252 2210 23 0.2109049611460989 2210 24 0.22623845409706433 2210 25 0.185164118758685 2211 1 -0.39677316017523623 2211 2 -0.10986468993825937 2211 3 -1.576131494659876 2211 4 -0.7884125874289438 2211 5 -0.4766956845479484 2211 6 -0.5440481952284273 2211 7 -0.505262842072824 2211 8 -0.20451065544341418 2211 9 0.04638261678660882 2211 10 0.005167046276170595 2211 11 -0.2600239125613584 2211 12 -0.24331138634995852 2211 13 -0.3615094358745239 2211 14 -0.796306210836832 2211 15 -0.4065366899977363 2211 16 -0.09714856044475795 2211 17 -0.24355997729829681 2211 18 -0.05018858814496241 2211 19 -0.05074479789053754 2211 20 -0.012153931790287231 2211 21 -0.33882137538959967 2211 22 -0.15972395257871597 2211 23 -0.4961519055569781 2211 24 -0.3033526054699607 2211 25 -0.1266304371141942 2212 1 -0.3182155628602743 2212 2 -0.2959009356198763 2212 3 -2.478674706943554 2212 4 -1.4398991098156806 2212 5 -0.6284350355361354 2212 6 -0.391798009659837 2212 7 -0.3379898163748578 2212 8 -0.2769130118478387 2212 9 -0.8249183535310394 2212 10 -0.12700769278492174 2212 11 -0.27686470985546663 2212 12 -0.026967580577256456 2212 13 -0.3524502369690481 2212 14 -0.08743007445296058 2212 15 0.10138387140624161 2212 16 -1.1032345126223149 2212 17 -1.3708578837834722 2212 18 -0.1937254309691454 2212 19 -0.1001496473307455 2212 20 -0.19575813192860636 2212 21 -0.02760499093590596 2212 22 -0.21190317870031805 2212 23 -0.05693389518643954 2212 24 -0.06350871992525242 2212 25 0.24381679834063874 2213 1 -2.956098393043277 2213 2 -1.4367213572422024 2213 3 -2.3000808411986817 2213 4 -1.0589530699293945 2213 5 -0.8091030867875801 2213 6 -0.6043833506940572 2213 7 -0.42364564393965765 2213 8 -0.1876540986690074 2213 9 -0.44864940887287474 2213 10 -0.17277835702112299 2213 11 -0.607779599388067 2213 12 -0.32655581271571066 2213 13 -0.596520071332904 2213 14 -0.07394564430255457 2213 15 -0.23138994562332726 2213 16 -1.594409999677211 2213 17 -0.9379265859679577 2213 18 -0.30858924326774684 2213 19 -0.036487034914230776 2213 20 -0.05231263177617507 2213 21 0.02000878729637634 2213 22 0.05214176154951389 2213 23 0.14605979118922363 2213 24 0.09482917271827063 2213 25 0.0369722815038358 2214 1 -0.9221216256631877 2214 2 -0.7822431892154 2214 3 -6.150833062330658 2214 4 -2.06126862026408 2214 5 -1.2497131411752918 2214 6 -0.6920083019638454 2214 7 -0.6618922505446392 2214 8 -0.4919781932756706 2214 9 -0.23338080062304556 2214 10 -0.2979659198684601 2214 11 -0.8068265785862181 2214 12 -0.5939100437760985 2214 13 -5.3306547831717275 2214 14 -4.160811217962337 2214 15 -2.2750297835842375 2214 16 -2.9166674981223295 2214 17 -3.528228257754691 2214 18 -1.4144386487629177 2214 19 -0.9745985582432084 2214 20 -0.5242289692778073 2214 21 -0.4016236715067194 2214 22 -0.4621076912194795 2214 23 -0.40909786959615246 2214 24 -0.24028998454217998 2214 25 -0.11779754532460629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1090.0 3 162.0 4 136.0 5 319.0 6 43.0 7 43.0 8 59.0 9 259.0 10 683.0 11 1761.0 12 3939.0 13 7205.0 14 11309.0 15 16261.0 16 20196.0 17 22815.0 18 23980.0 19 24021.0 20 24324.0 21 24163.0 22 24570.0 23 25508.0 24 26957.0 25 28688.0 26 31611.0 27 35586.0 28 41134.0 29 50259.0 30 61465.0 31 75730.0 32 96960.0 33 126355.0 34 167434.0 35 223701.0 36 307783.0 37 450711.0 38 814836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.660737642107726 21.637172008398085 13.449506392094184 22.252583957400002 2 14.037645328009706 21.68589334292953 41.86020574306192 22.416255585998847 3 18.43961314643737 28.84899009332044 28.94142981619619 23.769966944045994 4 11.51301981350509 18.51092819747454 38.30205534210284 31.67399664691753 5 12.328279140709055 39.917450553162865 34.17793245974036 13.57633784638772 6 29.537215597115196 39.10982339278482 18.134639085492157 13.218321924607832 7 24.882897387211127 34.56826533632708 23.53083870595115 17.017998570510645 8 23.69097398697038 38.7874038372684 21.10670886660814 16.414913309153075 9 24.543950523960987 18.180863195445443 22.128268190628013 35.14691808996556 10 14.915945379387422 31.612832891060922 32.38091043740026 21.0903112921514 11 31.303035159684832 28.059741576099906 21.921127142883957 18.716096121331304 12 20.416245380897085 29.546736820598863 31.580098682845005 18.45691911565905 13 25.907263559184067 25.354431554055346 26.809951770235585 21.928353116525003 14 19.729524165691718 24.460504889667966 28.390107821411153 27.41986312322916 15 23.577035025913766 30.621746339276335 24.038345951788152 21.762872683021744 16 20.46985898166177 32.47009866879786 26.722805436855072 20.3372369126853 17 19.760616734808508 31.33666786754179 29.078529291365758 19.824186106283946 18 20.104789440276413 30.638935241599885 29.94764863204008 19.308626686083628 19 20.41932862510997 29.666644645379662 30.41276916962059 19.50125755988978 20 22.017161029950582 28.89301610335604 29.04863495088855 20.041187915804827 21 21.914244480910693 29.738287705203852 28.737058232808504 19.610409581076954 22 20.535049349544806 29.302160867855992 29.320391013152058 20.842398769447144 23 19.84696229505297 30.117281737888003 29.526378734567267 20.50937723249176 24 20.240077895886817 29.571121334685515 29.174601810711113 21.014198958716552 25 21.209139119228034 28.45801730642186 28.06607775254777 22.266765821802338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3401.0 1 3401.0 2 3744.5 3 4088.0 4 4088.0 5 4088.0 6 5922.0 7 7756.0 8 7756.0 9 7756.0 10 11327.0 11 14898.0 12 14898.0 13 14898.0 14 22957.0 15 31016.0 16 31016.0 17 31016.0 18 46649.0 19 62282.0 20 62282.0 21 62282.0 22 86649.5 23 111017.0 24 111017.0 25 111017.0 26 143139.5 27 175262.0 28 175262.0 29 175262.0 30 208853.0 31 242444.0 32 242444.0 33 242444.0 34 269436.5 35 296429.0 36 296429.0 37 296429.0 38 313990.5 39 331552.0 40 331552.0 41 331552.0 42 330975.5 43 330399.0 44 330399.0 45 330399.0 46 353710.0 47 377021.0 48 377021.0 49 377021.0 50 339104.0 51 301187.0 52 301187.0 53 301187.0 54 251546.0 55 201905.0 56 201905.0 57 201905.0 58 166977.0 59 132049.0 60 132049.0 61 132049.0 62 106306.5 63 80564.0 64 80564.0 65 80564.0 66 60679.0 67 40794.0 68 40794.0 69 40794.0 70 29244.5 71 17695.0 72 17695.0 73 17695.0 74 12266.5 75 6838.0 76 6838.0 77 6838.0 78 4640.5 79 2443.0 80 2443.0 81 2443.0 82 1578.0 83 713.0 84 713.0 85 713.0 86 462.5 87 212.0 88 212.0 89 212.0 90 132.5 91 53.0 92 53.0 93 53.0 94 36.5 95 20.0 96 20.0 97 20.0 98 19.0 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10295607303748554 2 0.05458042694664177 3 0.057791040296444225 4 0.05306530603999342 5 0.048808537778457575 6 0.07048919646644945 7 0.06623242820491361 8 0.0632021863916169 9 0.06879370402329535 10 0.06828866372107924 11 0.062192105787184676 12 0.06374330100113418 13 0.06403189545954338 14 0.06590775943920324 15 0.06879370402329535 16 0.06414011838144684 17 0.06688176573633432 18 0.1453433841163382 19 0.07247328336801277 20 0.0658716851319021 21 0.06356292946462842 22 0.06922659571090915 23 0.06814436649187462 24 0.0795438475990384 25 0.07164357430008629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2772056.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.78673449680862 #Duplication Level Percentage of deduplicated Percentage of total 1 90.75130951457375 69.68496708933276 2 6.930717784427292 10.64374372770256 3 1.1522306759550973 2.6542809298092713 4 0.3848742290712339 1.1821294096942694 5 0.18915892802568537 0.7262448192004617 6 0.12061652994497098 0.5557049676486511 7 0.08414548609418572 0.45228799698733985 8 0.05557064597901089 0.3413670750885164 9 0.045381866019016955 0.31362527672747964 >10 0.2465078071565964 3.58247756301021 >50 0.019906994403254868 1.0652459044147553 >100 0.016174438145900107 2.4827460440536666 >500 0.0015133778684646565 0.8284494224468445 >1k 0.0015606709267344677 2.8127261457320167 >5k 2.364652919294648E-4 1.2632101502735587 >10k+ 9.458611677178592E-5 1.4107934778777484 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 20550 0.7413270150386573 No Hit CCCATGTACTCTGCGTTGATACCAC 18299 0.6601237493037658 No Hit GAGTACATGGGAAGCAGTGGTATCA 9138 0.3296470201179197 No Hit GTATCAACGCAGAGTACTTTTTTTT 7461 0.26915040677388913 No Hit CATGTACTCTGCGTTGATACCACTG 6647 0.23978592063075205 No Hit GCGTTGATACCACTGCTTCCCATGT 5796 0.2090866851174724 No Hit GTATCAACGCAGAGTACATGGGAAG 5743 0.20717474683051135 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4929 0.1778102606873743 No Hit GGTATCAACGCAGAGTACTTTTTTT 4744 0.1711365138366613 No Hit TATCAACGCAGAGTACTTTTTTTTT 4542 0.16384950376182877 No Hit ATTCCATTCCATTCCATTCCATTCC 4311 0.15551633877526283 No Hit GCTTCCCATGTACTCTGCGTTGATA 4262 0.15374869771750643 No Hit TATCAACGCAGAGTACATGGGAAGC 4193 0.15125957051372702 No Hit GGTATCAACGCAGAGTACATGGGAA 4100 0.14790465993471993 No Hit ACTCTGCGTTGATACCACTGCTTCC 3689 0.1330781196339468 No Hit GTGGTATCAACGCAGAGTACATGGG 3142 0.1133454735402171 No Hit GAATGGAATGGAATGGAATGGAATG 3095 0.11164998109706298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.21486146023024E-5 0.0 8 0.0 0.0 0.0 7.21486146023024E-5 0.0 9 0.0 0.0 0.0 1.442972292046048E-4 0.0 10 0.0 0.0 0.0 1.442972292046048E-4 0.0 11 0.0 0.0 0.0 1.8037153650575603E-4 0.0 12 0.0 0.0 0.0 2.525201511080584E-4 0.0 13 0.0 0.0 0.0 2.525201511080584E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCAT 60 4.1049303E-4 12.662598 6 CGCCCTA 125 1.4006218E-9 12.166199 16 ACTCGAC 130 2.6338967E-9 11.687919 11 GTCCTAG 200 0.0 11.40025 1 TAGGACT 255 0.0 11.172881 4 TGTCCCG 145 1.2259989E-9 11.13375 11 CCGTGCA 60 0.005891695 11.079773 9 TAGGACG 70 0.0014960435 10.853656 4 CTCGACT 145 1.4346369E-8 10.487533 12 ACACCGT 100 2.4087543E-5 10.446645 6 TACACCG 75 0.0026557574 10.130078 5 CCCGCCC 160 6.1281753E-9 10.098713 14 CTAGGAC 190 1.0004442E-10 9.996969 3 TATACTG 700 0.0 9.90396 5 GTCCCGC 165 1.0155418E-8 9.792337 12 GTCTAGG 240 1.8189894E-12 9.500208 1 GTCTTAC 180 4.1836756E-9 9.500208 1 CCTAGAC 230 3.6379788E-12 9.49712 3 GTTACAC 150 2.661891E-7 9.49712 3 TATTCCG 170 1.6749254E-8 9.496949 5 >>END_MODULE