FastQCFastQC Report
Wed 25 May 2016
SRR1294635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772056
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC207610.7489386938792001No Hit
GTATCAACGCAGAGTACTTTTTTTT205650.7418681296481745No Hit
GTACATGGGAAGCAGTGGTATCAAC204560.737936030152349No Hit
GGTATCAACGCAGAGTACTTTTTTT142800.5151411082604392No Hit
TATCAACGCAGAGTACTTTTTTTTT128210.4625086939080596No Hit
GAGTACATGGGAAGCAGTGGTATCA99420.35865076318804523No Hit
CATGTACTCTGCGTTGATACCACTG76630.2764374168487217No Hit
ACGCAGAGTACTTTTTTTTTTTTTT73800.26622838788249586No Hit
GCGTTGATACCACTGCTTCCCATGT60440.21803311332815786No Hit
GTATCAACGCAGAGTACATGGGAAG59300.21392064229582664No Hit
ACGCAGAGTACATGGGAAGCAGTGG58370.21056573171681958No Hit
GCTTCCCATGTACTCTGCGTTGATA48180.1738060125769465No Hit
GGTATCAACGCAGAGTACATGGGAA46730.1685752380182796No Hit
TATCAACGCAGAGTACATGGGAAGC45980.16586966497069322No Hit
ATTCCATTCCATTCCATTCCATTCC42310.15263039419117075No Hit
ACTCTGCGTTGATACCACTGCTTCC41850.15097097605531778No Hit
GTGGTATCAACGCAGAGTACATGGG36240.13073328965937198No Hit
GAATGGAATGGAATGGAATGGAATG34640.12496140049118779No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34200.12337413096993712No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34180.12330198235533481No Hit
CAGTGGTATCAACGCAGAGTACATG30590.11035130603422154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC350.002137937816.3229186
ACACCGT1251.7731509E-811.4260426
ACCGTAT600.005778633511.1086528
TACACCG1053.3752458E-610.8819455
CTTAGAC1355.6943463E-810.5553853
ATCGCAA1451.490298E-810.4619815
TTAGACA3250.010.254144
GCCCCGT1303.9497354E-710.21067214
CCTATAC3450.010.1882413
CCGTGTC856.462689E-410.0812559
GTCGCCC1701.5934347E-910.04667711
CTAGGAC2400.09.8956733
TCTCCGG1356.6217035E-79.83267719
CTAGACT2054.0017767E-119.7539394
AGCCCCG1401.0880467E-69.48133913
CTTACAC3850.09.3764723
GTCTTAG2850.09.3444981
CCCTTAG2650.09.3319041
CTGTCGC2054.0563464E-109.28895959
TGTCGCC1951.6170816E-99.27705110
Produced by FastQC (version 0.11.5)