FastQCFastQC Report
Wed 25 May 2016
SRR1294634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401945
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86132.1428304867581383No Hit
GGTATCAACGCAGAGTACTTTTTTT59321.475823806739728No Hit
TATCAACGCAGAGTACTTTTTTTTT54121.346452872905497No Hit
GTACTGGTTCACTATCGGTCAGTCA48161.1981738795108783No Hit
ATACAGGGTGACAGCCCCGTACACA34600.8608142905123837No Hit
GGATACCACGTGTCCCGCCCTACTC30250.7525905285549018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23500.5846571048277749No Hit
ACCCTGTATCGCGCGCCTTTCCAGA21870.544104292875891No Hit
GTTAATGATAGTGTGTCGAAACACA19990.497331724489669No Hit
GTTCACTATCGGTCAGTCAGGAGTA18100.4503103658460734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17810.44309544838224135No Hit
GTATTTAGCCTTGGAGGATGGTCCC16550.4117478759531777No Hit
CCCATATTCAGACAGGATACCACGT15400.38313699635522275No Hit
GCCTTGGAGGATGGTCCCCCCATAT14970.3724390152881613No Hit
ACTTAGATGTTTCAGTTCCCCCGGT14570.36248740499322046No Hit
GGTATCAACGCAGAGTACATGGGGG14030.3490527310950503No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG13980.3478087798081827No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13950.34706240903606217No Hit
GTCAGGAGTATTTAGCCTTGGAGGA13500.33586684745425366No Hit
CTATCGGTCAGTCAGGAGTATTTAG13320.3313886228215303No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13060.32492007612981877No Hit
TATTCAGACAGGATACCACGTGTCC12680.31546604634962494No Hit
TCTAAGTACCCCGAGGAAAAGAAAT12280.3055144360546841No Hit
ATCATTAACTGAATCCATAGGTTAA12180.3030265334809489No Hit
AGCGTACACGGTGGATGCCCTGGCA11820.2940700842155021No Hit
CGGTACTGGTTCACTATCGGTCAGT11790.29332371344338154No Hit
GGTTAATGAGGCGAACCGGGGGAAC11600.2885966985532846No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11340.2821281518615731No Hit
TCACGGTACTGGTTCACTATCGGTC11110.2764059759419821No Hit
GTACATGGGGGTTAAGCGACTAAGC10990.27342049285349984No Hit
CACACACACTGATTCAGGCTCTGGG10970.2729229123387528No Hit
GTACTTAGATGTTTCAGTTCCCCCG10640.2647128338454266No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10310.25650275535210043No Hit
TCATTAACCTATGGATTCAGTTAAT10190.25351727226361814No Hit
CATCTAAGTACCCCGAGGAAAAGAA10150.25252211123412405No Hit
GGTTCACTATCGGTCAGTCAGGAGT10120.2517757404620035No Hit
AGCTCACAGCATGTGCATTTTTGTG9340.23237010038686884No Hit
GTATCAACGCAGAGTACATGGGGGT9200.22888703678363956No Hit
CCCCAGTAGCGGCGAGCGAACGGGG9020.22440881215091615No Hit
GGGTGACAGCCCCGTACACAAAAAT8910.22167211931980743No Hit
GTCCCGCCCTACTCATCGAGCTCAC8840.21993058751819278No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8580.21346204082648124No Hit
GGGTTTCCCCATTCGGAAATCGCCG8570.21321325056910773No Hit
GCCCTACTCATCGAGCTCACAGCAT8300.20649591362002262No Hit
TTCCAGACGCTTCCACTAACACACA8290.2062471233626491No Hit
GTGCTAATCTGCGATAAGCGTCGGT8180.2035104305315404No Hit
CCTATGGATTCAGTTAATGATAGTG8050.20027615718568462No Hit
GCCTGAATCAGTGTGTGTGTTAGTG8020.19952978641356403No Hit
GGAGTATTTAGCCTTGGAGGATGGT7850.1953003520382142No Hit
GGGTACTTAGATGTTTCAGTTCCCC7740.19256365920710544No Hit
ACGCAGAGTACATGGGGGTTAAGCG7490.18634390277276744No Hit
ATCAACGCAGAGTACTTTTTTTTTT7330.18236325865479108No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7300.18161688788267052No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA7000.17415318016146486No Hit
ATGATAGTGTGTCGAAACACACTGG6950.17290922887459725No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC6880.17116769707298263No Hit
GAATCAGTGTGTGTGTTAGTGGAAG6790.16892858475662093No Hit
GTTATAACCGGCGATTTCCGAATGG6770.1684310042418739No Hit
GTCCCCCCATATTCAGACAGGATAC6730.1674358432123798No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6680.1661918919255122No Hit
GGTATCAACGCAGAGTACATGGGGA6680.1661918919255122No Hit
AGTCAGGAGTATTTAGCCTTGGAGG6400.15922576471905361No Hit
CTACTGGGGGAATCTCGGTTGATTT6090.15151326674047444No Hit
GTGGTATCAACGCAGAGTACATGGG6080.1512644764831009No Hit
GTATCGCGCGCCTTTCCAGACGCTT5930.1475326226224981No Hit
GTGGTATCAACGCAGAGTACTTTTT5770.14355197850452175No Hit
GATATGAACCGTTATAACCGGCGAT5690.1415616564455336No Hit
TAATGATAGTGTGTCGAAACACACT5670.14106407593078654No Hit
CACTATCATTAACTGAATCCATAGG5630.14006891490129247No Hit
TTTGTGTACGGGGCTGTCACCCTGT5400.13434673898170146No Hit
GGTGATATGAACCGTTATAACCGGC5390.13409794872432795No Hit
CTGTCACCCTGTATCGCGCGCCTTT5350.13310278769483386No Hit
CAGTAGCGGCGAGCGAACGGGGAGC5230.13011730460635162No Hit
GGGCTGTCACCCTGTATCGCGCGCC5210.12961972409160458No Hit
TAAGTACCCCGAGGAAAAGAAATCA5170.12862456306211048No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5160.12837577280473697No Hit
ATTTAGCCTTGGAGGATGGTCCCCC5140.12787819228998992No Hit
AACACACACACTGATTCAGGCTCTG5100.12688303126049583No Hit
TATCAACGCAGAGTACATGGGGGTT5050.12563907997362825No Hit
CATTAACCTATGGATTCAGTTAATG4970.12364875791464006No Hit
CACTGATTCAGGCTCTGGGCTGCTC4960.12339996765726655No Hit
GTGGTATCCTGTCTGAATATGGGGG4900.12190722611302542No Hit
TCCCCCAGTAGCGGCGAGCGAACGG4890.12165843585565188No Hit
CGTCTGGAAAGGCGCGCGATACAGG4880.12140964559827837No Hit
GAGTACATGGGGGTTAAGCGACTAA4790.1191705332819167No Hit
CTATCATTAACTGAATCCATAGGTT4740.11792658199504909No Hit
GTACGGGGCTGTCACCCTGTATCGC4610.1146923086491933No Hit
CTAACACACACACTGATTCAGGCTC4600.11444351839181978No Hit
GGTATCAACGCAGAGTACATGGGCA4560.1134483573623257No Hit
CCGTTATAACCGGCGATTTCCGAAT4530.11270198659020511No Hit
TCGGTAAGGTGATATGAACCGTTAT4470.111209245045964No Hit
GTACACAAAAATGCACATGCTGTGA4450.11071166453121696No Hit
GTGCATTTTTGTGTACGGGGCTGTC4340.10797497170010822No Hit
CAGGGTGACAGCCCCGTACACAAAA4300.10697981067061414No Hit
ATCCATAGGTTAATGAGGCGAACCG4280.10648223015586711No Hit
GCCCCGTACACAAAAATGCACATGC4280.10648223015586711No Hit
CTACTCATCGAGCTCACAGCATGTG4260.10598464964112005No Hit
CTGTGAGCTCGATGAGTAGGGCGGG4170.10374553732475837No Hit
CAGTTAATGATAGTGTGTCGAAACA4120.10250158603789077No Hit
GGAGGATGGTCCCCCCATATTCAGA4110.10225279578051723No Hit
GTTCATATCACCTTACCGACGCTTA4080.10150642500839667No Hit
CCACGTGTCCCGCCCTACTCATCGA4050.1007600542362761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.016.0105231
GTACAAA1400.015.6056091
TACATGA807.679773E-915.4340622
GTACATA1250.015.1985061
TACAAAG508.704857E-515.1966162
CATAGAA953.0377123E-1014.996664
TACATAA1151.8189894E-1214.8662532
TAGGTGT400.00524744514.25924918
CATCTTA400.005256126614.25569813
GGTTATA903.6168785E-813.7208751
CCCGAAC500.001490271813.30697516
TGAACCA500.001497282213.29869419
CAACCCC1301.4551915E-1113.15910512
AGGGTGA6100.012.9217425
TGTCCCG6650.012.8526811
ACATAGA905.386137E-712.6638463
ACATGAA1207.348717E-1012.6638453
GTGTCCC6550.012.6139410
AATCGCC1600.012.47684318
AAATCGC1600.012.47528917
Produced by FastQC (version 0.11.5)