##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294634_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401945 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.251497095373747 31.0 26.0 31.0 15.0 34.0 2 27.03248951971041 31.0 26.0 31.0 12.0 34.0 3 27.16221373571011 31.0 26.0 33.0 12.0 34.0 4 29.773891950391224 35.0 27.0 37.0 10.0 37.0 5 31.280125390289715 35.0 30.0 37.0 17.0 37.0 6 30.834146462824517 35.0 30.0 37.0 13.0 37.0 7 30.85481595740711 35.0 30.0 37.0 13.0 37.0 8 30.501058602545125 35.0 28.0 37.0 13.0 37.0 9 30.64543158889898 37.0 27.0 39.0 10.0 39.0 10 31.100911816293273 37.0 27.0 39.0 10.0 39.0 11 31.29158964534949 37.0 27.0 39.0 11.0 39.0 12 31.322593887223377 37.0 27.0 39.0 12.0 39.0 13 30.85182052270833 37.0 27.0 39.0 10.0 39.0 14 31.214158653547127 36.0 27.0 40.0 10.0 41.0 15 31.276920971774747 36.0 27.0 40.0 10.0 41.0 16 31.510482777494435 36.0 27.0 40.0 10.0 41.0 17 31.714538058689623 37.0 27.0 40.0 10.0 41.0 18 31.689320678202243 37.0 27.0 40.0 11.0 41.0 19 32.07729415716081 37.0 27.0 40.0 12.0 41.0 20 32.254395501872146 37.0 28.0 40.0 13.0 41.0 21 32.336498774707984 37.0 28.0 40.0 13.0 41.0 22 32.51593625993606 37.0 28.0 40.0 15.0 41.0 23 32.71336128077225 37.0 28.0 40.0 16.0 41.0 24 32.93879013297839 37.0 29.0 40.0 18.0 41.0 25 31.751831718269912 35.0 27.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2772253716629116 1101 2 1.1555185439787259 1101 3 1.4338549581785607 1101 4 1.33256138867063 1101 5 1.1667107905637657 1101 6 0.8659972666769988 1101 7 1.1928029380413498 1101 8 1.125811979467457 1101 9 1.5226655340831101 1101 10 1.3946578837037613 1101 11 1.0975077897269827 1101 12 0.9615033574369498 1101 13 1.6089265717289685 1101 14 1.4017572503908795 1101 15 1.5034739310794087 1101 16 1.1227207710679785 1101 17 1.5495130855559935 1101 18 1.377521649212369 1101 19 1.3273338262528398 1101 20 1.0432599953382393 1101 21 0.993000269244888 1101 22 0.7608351032555163 1101 23 1.0802892202700676 1101 24 1.0431647882013237 1101 25 1.0971248552292217 1102 1 1.014600382315411 1102 2 0.9620176349652922 1102 3 1.0967938412283011 1102 4 1.232832672368044 1102 5 0.8884852131982051 1102 6 0.7651003260753484 1102 7 0.5998030516680295 1102 8 0.545032287395756 1102 9 1.0488191999638588 1102 10 0.5788307524933529 1102 11 0.7223302480401941 1102 12 0.7864117459265678 1102 13 0.7816266967183125 1102 14 0.7852171117663289 1102 15 0.9074753656162393 1102 16 1.0699784621738466 1102 17 0.8611662117100138 1102 18 1.2933988471784517 1102 19 0.950602794806656 1102 20 0.4011360996475304 1102 21 0.7948335647026639 1102 22 0.6944345123967821 1102 23 0.9640509025130584 1102 24 0.9780904337428993 1102 25 1.0138329482894726 1103 1 0.48178524242823784 1103 2 0.35070746343526693 1103 3 0.6571926700132735 1103 4 -0.060450738759932676 1103 5 0.21173268606553108 1103 6 -0.21778604602167206 1103 7 -0.6001017334183096 1103 8 -0.2853688322743544 1103 9 -0.4239496926795354 1103 10 -0.49228154383263956 1103 11 -0.32070617703354287 1103 12 0.4343671309384476 1103 13 -0.13769656149307608 1103 14 -0.5386514057446234 1103 15 0.07733912954052613 1103 16 0.2604387153166563 1103 17 0.2658976054272628 1103 18 0.18979714619623778 1103 19 0.20566958842654515 1103 20 -0.44697642576697305 1103 21 -0.6045196181958801 1103 22 -0.8961413588554699 1103 23 -0.4569618834896332 1103 24 -0.7403186181170156 1103 25 -0.1689975834822377 1104 1 0.633983825951578 1104 2 0.7082744035481277 1104 3 1.2664904172825615 1104 4 1.2927653967568205 1104 5 1.0049526966527758 1104 6 0.6524752098471573 1104 7 0.36840639903990535 1104 8 0.8097693382079854 1104 9 1.1586377472482212 1104 10 0.7705970107660676 1104 11 0.8867657892533458 1104 12 1.300236063598721 1104 13 1.1026045176448562 1104 14 1.295284524116603 1104 15 1.6407761094766578 1104 16 0.8385715541171841 1104 17 0.9156362111630614 1104 18 1.0066775760522688 1104 19 0.6067895529973271 1104 20 0.7380166717637096 1104 21 -0.06467731972549728 1104 22 0.22738862277254412 1104 23 0.22513702076498987 1104 24 -0.31979216211117745 1104 25 -2.2273952418324967 1105 1 0.22799587587172 1105 2 0.1973347831839618 1105 3 0.4691601381071848 1105 4 0.3909652440489211 1105 5 0.2289831848845978 1105 6 0.07514599762652452 1105 7 0.4100142998397871 1105 8 0.6785922537285103 1105 9 0.8502428139495635 1105 10 -0.8920018909373937 1105 11 -0.08707381410622972 1105 12 0.5828973821547301 1105 13 0.30505751328701436 1105 14 0.9607542009293439 1105 15 0.06369863189103953 1105 16 0.4921246801278727 1105 17 0.1906671361794814 1105 18 0.027444849882872546 1105 19 -1.7330372002260432 1105 20 -0.5396616347123739 1105 21 -1.4732643048781533 1105 22 -0.4815044450224235 1105 23 -0.09828697037929146 1105 24 -0.3544098422181392 1105 25 -0.3831956412736801 1106 1 0.05822951031866097 1106 2 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0.505496629741419 1107 14 0.27288926121357093 1107 15 0.5683628094659312 1107 16 0.37522263195624106 1107 17 0.08185709585881895 1107 18 -0.22885617608861963 1107 19 0.20062801201084568 1107 20 0.14151489567733933 1107 21 0.3074135872972761 1107 22 -0.048350004912300903 1107 23 0.16150665472545 1107 24 -0.22498034926810107 1107 25 0.10863282455705914 1108 1 -0.7306729482091292 1108 2 -0.6032472691846991 1108 3 0.21367028951142686 1108 4 -0.5522421117061107 1108 5 -0.2492596783122245 1108 6 -0.0620536659195956 1108 7 -0.2685085135310423 1108 8 -0.4204064107293739 1108 9 0.05046497624361379 1108 10 0.04374280220896409 1108 11 -0.5130025937546208 1108 12 -0.39141108954847326 1108 13 -0.45940711410957746 1108 14 -0.3868784222551582 1108 15 -0.5807935410616629 1108 16 -0.38803449337702745 1108 17 -0.402868968403741 1108 18 -0.11643894511141539 1108 19 -0.44939983843538656 1108 20 -0.3023476795814517 1108 21 -0.14324574854781247 1108 22 -0.5230186158947703 1108 23 -0.43136988956614886 1108 24 -0.1619585445591909 1108 25 -0.49920159984618806 1109 1 -0.491142328480489 1109 2 -0.8487093153893177 1109 3 -0.500576135131034 1109 4 -0.6130170780916373 1109 5 -0.4887475159848833 1109 6 -0.16253954784112423 1109 7 -0.31669944373517467 1109 8 -0.28222342576421156 1109 9 -0.09432172464869737 1109 10 -0.046750413779300004 1109 11 -0.3383540177859139 1109 12 -0.1491149154866207 1109 13 -0.2317729618054578 1109 14 -0.20479799198990634 1109 15 -0.9293022808245297 1109 16 -0.5856723127145145 1109 17 -0.3652390943052204 1109 18 -0.033952085314322034 1109 19 -0.03744225378803634 1109 20 -0.14419158619433858 1109 21 -0.22029317551272953 1109 22 -0.21074016582753785 1109 23 -0.3888811962523704 1109 24 -0.14905364295791657 1109 25 -0.07273784235933078 1110 1 -0.23888321296134762 1110 2 -0.32067999240527456 1110 3 -0.24371254450289115 1110 4 -0.27024321323929 1110 5 -0.21207973133952152 1110 6 -0.2090298932965382 1110 7 -0.1415920379248803 1110 8 -0.05396890992884096 1110 9 0.4196130812222876 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-0.31787407981822113 2207 11 0.20635348228881867 2207 12 -0.058070763990492935 2207 13 0.5108749322548647 2207 14 0.6256716220955241 2207 15 0.5952007532830237 2207 16 0.6824501277243051 2207 17 0.5357772569936188 2207 18 0.5171315245263486 2207 19 -0.6840598964508757 2207 20 -0.0336334897605326 2207 21 -0.5472115385892877 2207 22 0.3783250899043864 2207 23 -0.30819486019880316 2207 24 -1.295037560693249 2207 25 -0.21963647626361293 2208 1 0.2292280418898791 2208 2 0.04194109111474731 2208 3 0.7121609922402392 2208 4 0.5229208923991777 2208 5 0.5752392349937381 2208 6 -0.017372429503272002 2208 7 0.35806719282008004 2208 8 0.3754098172348854 2208 9 -0.3613196168498405 2208 10 -0.18527796572148603 2208 11 0.31601213698674613 2208 12 0.6909784298912776 2208 13 0.5202294471075213 2208 14 0.5861836400486347 2208 15 0.3451180744901343 2208 16 0.5284047725007817 2208 17 0.15064467564359063 2208 18 0.48586122392988074 2208 19 -1.5492609830864374 2208 20 -0.025959126091954943 2208 21 -0.3986731815350133 2208 22 -0.019758533788994725 2208 23 0.17898992690330573 2208 24 0.3486729454190751 2208 25 0.9771567676978812 2209 1 0.20919150108598217 2209 2 -1.0567888931400304 2209 3 -0.26563229969999114 2209 4 0.10368943759985783 2209 5 -0.08958058616427778 2209 6 0.09256488667865881 2209 7 0.03511481469702815 2209 8 0.12104720174514938 2209 9 -2.125298702438503 2209 10 -0.6321972491652339 2209 11 -0.15000045919281035 2209 12 0.06375256019043718 2209 13 0.150816773687243 2209 14 0.4951599033800882 2209 15 0.23236295729219236 2209 16 0.5455061658046034 2209 17 0.3896051937505973 2209 18 0.3674749608955068 2209 19 0.25395787553333093 2209 20 0.5584219083395823 2209 21 0.3590018726820361 2209 22 0.2694856954377087 2209 23 0.6346507807103805 2209 24 0.5359029742358103 2209 25 0.3392048351943906 2210 1 -0.06400236191059605 2210 2 0.00600169015304175 2210 3 0.14933149221127096 2210 4 0.13437156153769791 2210 5 0.27360549419077174 2210 6 0.08201926275094351 2210 7 0.2854236611591112 2210 8 0.18028207260827145 2210 9 0.36909577043942576 2210 10 -1.0881949461977065 2210 11 -0.5724908466179137 2210 12 -2.222268195449086 2210 13 -0.6179317254780763 2210 14 -0.18230891542323846 2210 15 -0.4085433587987808 2210 16 -0.29002115878941126 2210 17 -0.31953955470227413 2210 18 0.1280314342305573 2210 19 -0.04006974271797503 2210 20 0.46820980965915737 2210 21 0.5254679458620117 2210 22 0.17184392261561499 2210 23 0.21360553434203666 2210 24 0.1179707844782314 2210 25 0.44902398280708766 2211 1 -0.5956162918585619 2211 2 -0.14364497333867376 2211 3 -1.3936161240614346 2211 4 -0.732105988504749 2211 5 -0.3850468871700983 2211 6 -0.41263410067540107 2211 7 -0.6052623943985829 2211 8 -0.05156524579096455 2211 9 -0.0962435302234681 2211 10 -0.20439074402237267 2211 11 -0.11096444473423972 2211 12 -0.5665664475500272 2211 13 -0.38401378710564416 2211 14 -0.5502817488891907 2211 15 -0.5568455983221838 2211 16 0.0070048393233683726 2211 17 -0.24682973434334698 2211 18 -0.3966828941638525 2211 19 -0.22737506746513958 2211 20 0.37727113846473515 2211 21 -0.1681261475466158 2211 22 0.02260546706147437 2211 23 -0.2639223228916734 2211 24 -0.22688061895841116 2211 25 -0.3006824328609987 2212 1 -0.4740356656819209 2212 2 -0.806498697460686 2212 3 -2.5626591058301926 2212 4 -1.9637536283930253 2212 5 -0.9937921602810498 2212 6 -0.4389925021008665 2212 7 -0.14353759607376304 2212 8 -0.4928510246424622 2212 9 -0.8296632454558406 2212 10 -0.10600967303856024 2212 11 -0.2855512585427711 2212 12 0.2254845752138266 2212 13 -0.6670532377297711 2212 14 -0.2513537731845048 2212 15 0.0016263850040516559 2212 16 -1.0044583746685518 2212 17 -0.9356159115053622 2212 18 -0.5082163408666567 2212 19 -0.24887570638771805 2212 20 -0.2047434348227739 2212 21 -0.2795685518464168 2212 22 -0.22648654754432584 2212 23 0.18516709050261682 2212 24 -0.17046034121405285 2212 25 0.2173146993033086 2213 1 -3.694875543767491 2213 2 -1.806876646066609 2213 3 -2.783887334776896 2213 4 -1.5093969905406048 2213 5 -1.4729096329491256 2213 6 -1.2475476635265288 2213 7 -1.0248790709055058 2213 8 -0.7471341532247386 2213 9 -0.598773055129243 2213 10 -1.0130481856547604 2213 11 -1.3135298576363894 2213 12 -0.9374234099040137 2213 13 -1.1766059754299718 2213 14 -0.8972922126381775 2213 15 -1.2613616974789004 2213 16 -2.1338745654320412 2213 17 -1.5445760674004916 2213 18 -1.014705614407827 2213 19 -0.6311464493514585 2213 20 -0.6435521685562478 2213 21 -0.5161123678790673 2213 22 0.03939509559590704 2213 23 -0.06209451581363368 2213 24 0.10303922015751965 2213 25 -0.18244223466664522 2214 1 -0.6047498586626077 2214 2 -0.44079034669686834 2214 3 -5.434584729360623 2214 4 -1.1882029453585368 2214 5 -0.9683532727545447 2214 6 -0.2836977225904569 2214 7 -0.4653932572540356 2214 8 0.08513896425011325 2214 9 0.3372654794983596 2214 10 0.11498480209955986 2214 11 -0.34939009990855396 2214 12 -0.3774826939464795 2214 13 -4.317299187847336 2214 14 -2.619442286376376 2214 15 -1.6190983383922237 2214 16 -2.3098043637342265 2214 17 -2.8699522789447407 2214 18 -0.7948960864056538 2214 19 0.12822857357708273 2214 20 0.12656074993552835 2214 21 0.7713308610468488 2214 22 0.29781995967550046 2214 23 0.5445067036180333 2214 24 0.5286150017457203 2214 25 1.000388138133136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 149.0 3 32.0 4 21.0 5 41.0 6 7.0 7 4.0 8 13.0 9 53.0 10 163.0 11 564.0 12 1699.0 13 3523.0 14 6115.0 15 8823.0 16 11473.0 17 12650.0 18 12042.0 19 11167.0 20 9998.0 21 8660.0 22 7682.0 23 6482.0 24 6016.0 25 5460.0 26 5407.0 27 5325.0 28 5939.0 29 6851.0 30 8081.0 31 10111.0 32 12946.0 33 16437.0 34 21643.0 35 28475.0 36 38124.0 37 54599.0 38 75170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.04942397633148 20.85763523979818 12.221513848973716 17.871426934896625 2 17.815120611933498 26.689201803142804 34.2614557067578 21.234221878165894 3 20.387532640812903 35.429315370617914 23.00502089758813 21.178131090981054 4 11.565602885426753 25.142441809813736 28.615557707290485 34.67639759746903 5 12.970679012345679 45.52245121465552 24.637096774193548 16.869772998805256 6 25.100391108322818 42.24990601948322 15.75661162271366 16.893091249480307 7 23.436115232566245 43.83806498511297 14.719984465710045 18.005835316610735 8 23.519878916722885 40.229072433390506 17.529194163905533 18.721854485981076 9 23.72815108581728 26.504746327291194 16.374020050836613 33.393082536054905 10 18.643308280410174 37.4810484884847 19.632645643455163 24.24299758764996 11 27.87710415079685 38.11892279428238 14.837468198134953 19.16650485678581 12 20.651137548948586 38.54945394165254 20.777601027640237 20.02180748175864 13 27.582275329848144 33.10580034851879 20.73661936768733 18.575304953945732 14 20.829101192965684 35.53205472904344 22.03501224831212 21.603831829678754 15 25.729180151561927 34.29486860318061 21.10813475269117 18.8678164925663 16 19.103003266151518 35.74568828168565 20.217527682625665 24.93378076953716 17 19.923025410078893 33.777953700730166 20.80454877007451 25.494472119116434 18 19.741189015730964 29.87961352753292 26.185552626727194 24.19364483000892 19 21.873233931421147 31.534920544030037 27.214945639319936 19.37689988522888 20 23.923742758773493 25.75698356705245 28.72442387594506 21.594849798228996 21 26.986651929520594 27.174102453037797 26.090474127844743 19.74877148959687 22 25.92982613371572 27.585339865766468 25.112027245025992 21.372806755491823 23 25.098771425370625 27.68388164855546 27.004991473418226 20.21235545265569 24 24.66443412551197 26.309958046484994 26.58732431187521 22.43828351612783 25 23.37739935768168 29.774939627057034 27.62889934523365 19.218761670027636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2302.0 1 2302.0 2 2439.0 3 2576.0 4 2576.0 5 2576.0 6 3337.0 7 4098.0 8 4098.0 9 4098.0 10 4623.5 11 5149.0 12 5149.0 13 5149.0 14 5376.5 15 5604.0 16 5604.0 17 5604.0 18 7084.5 19 8565.0 20 8565.0 21 8565.0 22 10678.5 23 12792.0 24 12792.0 25 12792.0 26 17588.0 27 22384.0 28 22384.0 29 22384.0 30 25330.5 31 28277.0 32 28277.0 33 28277.0 34 29953.5 35 31630.0 36 31630.0 37 31630.0 38 28890.0 39 26150.0 40 26150.0 41 26150.0 42 30389.5 43 34629.0 44 34629.0 45 34629.0 46 46662.5 47 58696.0 48 58696.0 49 58696.0 50 55784.5 51 52873.0 52 52873.0 53 52873.0 54 46116.0 55 39359.0 56 39359.0 57 39359.0 58 32537.5 59 25716.0 60 25716.0 61 25716.0 62 24591.5 63 23467.0 64 23467.0 65 23467.0 66 15929.5 67 8392.0 68 8392.0 69 8392.0 70 7177.0 71 5962.0 72 5962.0 73 5962.0 74 4202.0 75 2442.0 76 2442.0 77 2442.0 78 1587.0 79 732.0 80 732.0 81 732.0 82 416.5 83 101.0 84 101.0 85 101.0 86 67.5 87 34.0 88 34.0 89 34.0 90 21.5 91 9.0 92 9.0 93 9.0 94 6.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09926731269203498 2 0.050255631989451294 3 0.055729017651668766 4 0.050255631989451294 5 0.046026197614101434 6 0.06617820846135665 7 0.06294393511550088 8 0.05921208125489806 9 0.06617820846135665 10 0.06518304743186257 11 0.05946087151227158 12 0.06169998382863327 13 0.06095361305651271 14 0.06393909614499496 15 0.06493425717448904 16 0.06194877408600679 17 0.0631927253728744 18 0.14205923696028064 19 0.06866611103509188 20 0.06269514485812736 21 0.05946087151227158 22 0.06393909614499496 23 0.06468546691711552 24 0.0771249797857916 25 0.06841732077771834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 401945.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.00584879523578 #Duplication Level Percentage of deduplicated Percentage of total 1 86.5516904667237 32.89470460850351 2 6.800334350144322 5.169049581372661 3 2.249647946844111 2.564993391308097 4 1.1711498681433723 1.7804217922086931 5 0.6517038246910706 1.2384278510242839 6 0.41160175931003434 0.9385964536914119 7 0.30547160762821796 0.8126795411528951 8 0.22301389769216953 0.6780665979939822 9 0.15258002672983573 0.5219040082560453 >10 1.091559413001543 8.02217763834105 >50 0.14795048537079739 3.9720757394965673 >100 0.19134929441289794 15.908545699580534 >500 0.028274981648641277 7.371106954602445 >1k 0.02169940452105028 13.1397884881874 >5k 0.0019726731382772983 4.98746165428042 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8613 2.1428304867581383 No Hit GGTATCAACGCAGAGTACTTTTTTT 5932 1.475823806739728 No Hit TATCAACGCAGAGTACTTTTTTTTT 5412 1.346452872905497 No Hit GTACTGGTTCACTATCGGTCAGTCA 4816 1.1981738795108783 No Hit ATACAGGGTGACAGCCCCGTACACA 3460 0.8608142905123837 No Hit GGATACCACGTGTCCCGCCCTACTC 3025 0.7525905285549018 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2350 0.5846571048277749 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 2187 0.544104292875891 No Hit GTTAATGATAGTGTGTCGAAACACA 1999 0.497331724489669 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1810 0.4503103658460734 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1781 0.44309544838224135 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1655 0.4117478759531777 No Hit CCCATATTCAGACAGGATACCACGT 1540 0.38313699635522275 No Hit GCCTTGGAGGATGGTCCCCCCATAT 1497 0.3724390152881613 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1457 0.36248740499322046 No Hit GGTATCAACGCAGAGTACATGGGGG 1403 0.3490527310950503 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1398 0.3478087798081827 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 1395 0.34706240903606217 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1350 0.33586684745425366 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1332 0.3313886228215303 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1306 0.32492007612981877 No Hit TATTCAGACAGGATACCACGTGTCC 1268 0.31546604634962494 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 1228 0.3055144360546841 No Hit ATCATTAACTGAATCCATAGGTTAA 1218 0.3030265334809489 No Hit AGCGTACACGGTGGATGCCCTGGCA 1182 0.2940700842155021 No Hit CGGTACTGGTTCACTATCGGTCAGT 1179 0.29332371344338154 No Hit GGTTAATGAGGCGAACCGGGGGAAC 1160 0.2885966985532846 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1134 0.2821281518615731 No Hit TCACGGTACTGGTTCACTATCGGTC 1111 0.2764059759419821 No Hit GTACATGGGGGTTAAGCGACTAAGC 1099 0.27342049285349984 No Hit CACACACACTGATTCAGGCTCTGGG 1097 0.2729229123387528 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1064 0.2647128338454266 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 1031 0.25650275535210043 No Hit TCATTAACCTATGGATTCAGTTAAT 1019 0.25351727226361814 No Hit CATCTAAGTACCCCGAGGAAAAGAA 1015 0.25252211123412405 No Hit GGTTCACTATCGGTCAGTCAGGAGT 1012 0.2517757404620035 No Hit AGCTCACAGCATGTGCATTTTTGTG 934 0.23237010038686884 No Hit GTATCAACGCAGAGTACATGGGGGT 920 0.22888703678363956 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 902 0.22440881215091615 No Hit GGGTGACAGCCCCGTACACAAAAAT 891 0.22167211931980743 No Hit GTCCCGCCCTACTCATCGAGCTCAC 884 0.21993058751819278 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 858 0.21346204082648124 No Hit GGGTTTCCCCATTCGGAAATCGCCG 857 0.21321325056910773 No Hit GCCCTACTCATCGAGCTCACAGCAT 830 0.20649591362002262 No Hit TTCCAGACGCTTCCACTAACACACA 829 0.2062471233626491 No Hit GTGCTAATCTGCGATAAGCGTCGGT 818 0.2035104305315404 No Hit CCTATGGATTCAGTTAATGATAGTG 805 0.20027615718568462 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 802 0.19952978641356403 No Hit GGAGTATTTAGCCTTGGAGGATGGT 785 0.1953003520382142 No Hit GGGTACTTAGATGTTTCAGTTCCCC 774 0.19256365920710544 No Hit ACGCAGAGTACATGGGGGTTAAGCG 749 0.18634390277276744 No Hit ATCAACGCAGAGTACTTTTTTTTTT 733 0.18236325865479108 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 730 0.18161688788267052 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 700 0.17415318016146486 No Hit ATGATAGTGTGTCGAAACACACTGG 695 0.17290922887459725 No Hit GCTCTGGGCTGCTCCCCGTTCGCTC 688 0.17116769707298263 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 679 0.16892858475662093 No Hit GTTATAACCGGCGATTTCCGAATGG 677 0.1684310042418739 No Hit GTCCCCCCATATTCAGACAGGATAC 673 0.1674358432123798 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 668 0.1661918919255122 No Hit GGTATCAACGCAGAGTACATGGGGA 668 0.1661918919255122 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 640 0.15922576471905361 No Hit CTACTGGGGGAATCTCGGTTGATTT 609 0.15151326674047444 No Hit GTGGTATCAACGCAGAGTACATGGG 608 0.1512644764831009 No Hit GTATCGCGCGCCTTTCCAGACGCTT 593 0.1475326226224981 No Hit GTGGTATCAACGCAGAGTACTTTTT 577 0.14355197850452175 No Hit GATATGAACCGTTATAACCGGCGAT 569 0.1415616564455336 No Hit TAATGATAGTGTGTCGAAACACACT 567 0.14106407593078654 No Hit CACTATCATTAACTGAATCCATAGG 563 0.14006891490129247 No Hit TTTGTGTACGGGGCTGTCACCCTGT 540 0.13434673898170146 No Hit GGTGATATGAACCGTTATAACCGGC 539 0.13409794872432795 No Hit CTGTCACCCTGTATCGCGCGCCTTT 535 0.13310278769483386 No Hit CAGTAGCGGCGAGCGAACGGGGAGC 523 0.13011730460635162 No Hit GGGCTGTCACCCTGTATCGCGCGCC 521 0.12961972409160458 No Hit TAAGTACCCCGAGGAAAAGAAATCA 517 0.12862456306211048 No Hit GATTCAGGCTCTGGGCTGCTCCCCG 516 0.12837577280473697 No Hit ATTTAGCCTTGGAGGATGGTCCCCC 514 0.12787819228998992 No Hit AACACACACACTGATTCAGGCTCTG 510 0.12688303126049583 No Hit TATCAACGCAGAGTACATGGGGGTT 505 0.12563907997362825 No Hit CATTAACCTATGGATTCAGTTAATG 497 0.12364875791464006 No Hit CACTGATTCAGGCTCTGGGCTGCTC 496 0.12339996765726655 No Hit GTGGTATCCTGTCTGAATATGGGGG 490 0.12190722611302542 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 489 0.12165843585565188 No Hit CGTCTGGAAAGGCGCGCGATACAGG 488 0.12140964559827837 No Hit GAGTACATGGGGGTTAAGCGACTAA 479 0.1191705332819167 No Hit CTATCATTAACTGAATCCATAGGTT 474 0.11792658199504909 No Hit GTACGGGGCTGTCACCCTGTATCGC 461 0.1146923086491933 No Hit CTAACACACACACTGATTCAGGCTC 460 0.11444351839181978 No Hit GGTATCAACGCAGAGTACATGGGCA 456 0.1134483573623257 No Hit CCGTTATAACCGGCGATTTCCGAAT 453 0.11270198659020511 No Hit TCGGTAAGGTGATATGAACCGTTAT 447 0.111209245045964 No Hit GTACACAAAAATGCACATGCTGTGA 445 0.11071166453121696 No Hit GTGCATTTTTGTGTACGGGGCTGTC 434 0.10797497170010822 No Hit CAGGGTGACAGCCCCGTACACAAAA 430 0.10697981067061414 No Hit ATCCATAGGTTAATGAGGCGAACCG 428 0.10648223015586711 No Hit GCCCCGTACACAAAAATGCACATGC 428 0.10648223015586711 No Hit CTACTCATCGAGCTCACAGCATGTG 426 0.10598464964112005 No Hit CTGTGAGCTCGATGAGTAGGGCGGG 417 0.10374553732475837 No Hit CAGTTAATGATAGTGTGTCGAAACA 412 0.10250158603789077 No Hit GGAGGATGGTCCCCCCATATTCAGA 411 0.10225279578051723 No Hit GTTCATATCACCTTACCGACGCTTA 408 0.10150642500839667 No Hit CCACGTGTCCCGCCCTACTCATCGA 405 0.1007600542362761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1240 0.0 16.010523 1 GTACAAA 140 0.0 15.605609 1 TACATGA 80 7.679773E-9 15.434062 2 GTACATA 125 0.0 15.198506 1 TACAAAG 50 8.704857E-5 15.196616 2 CATAGAA 95 3.0377123E-10 14.99666 4 TACATAA 115 1.8189894E-12 14.866253 2 TAGGTGT 40 0.005247445 14.259249 18 CATCTTA 40 0.0052561266 14.255698 13 GGTTATA 90 3.6168785E-8 13.720875 1 CCCGAAC 50 0.0014902718 13.306975 16 TGAACCA 50 0.0014972822 13.298694 19 CAACCCC 130 1.4551915E-11 13.159105 12 AGGGTGA 610 0.0 12.921742 5 TGTCCCG 665 0.0 12.85268 11 ACATAGA 90 5.386137E-7 12.663846 3 ACATGAA 120 7.348717E-10 12.663845 3 GTGTCCC 655 0.0 12.61394 10 AATCGCC 160 0.0 12.476843 18 AAATCGC 160 0.0 12.475289 17 >>END_MODULE