FastQCFastQC Report
Wed 25 May 2016
SRR1294634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401945
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT240275.977683513913595No Hit
GGTATCAACGCAGAGTACTTTTTTT177624.419012551468485No Hit
TATCAACGCAGAGTACTTTTTTTTT160033.9813904887484606No Hit
ACGCAGAGTACTTTTTTTTTTTTTT84822.110238963042207No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50601.2588787023100174No Hit
GTACTGGTTCACTATCGGTCAGTCA48251.20041299182724No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41861.04143601736556No Hit
ATACAGGGTGACAGCCCCGTACACA39450.9814775653385412No Hit
GGATACCACGTGTCCCGCCCTACTC32220.8016022092574855No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31740.7896602769035566No Hit
ACCCTGTATCGCGCGCCTTTCCAGA24930.6202341116321884No Hit
ATCAACGCAGAGTACTTTTTTTTTT24580.6115264526241153No Hit
GTTAATGATAGTGTGTCGAAACACA20820.5179813158516713No Hit
CCCATATTCAGACAGGATACCACGT17790.44259786786749433No Hit
GTTCACTATCGGTCAGTCAGGAGTA17030.4236898083071067No Hit
GTGGTATCAACGCAGAGTACTTTTT16980.42244585702023907No Hit
GTATTTAGCCTTGGAGGATGGTCCC16200.4030402169451044No Hit
ACTTAGATGTTTCAGTTCCCCCGGT16180.40254263643035737No Hit
GGTATCAACGCAGAGTACATGGGGG15700.3906007040764284No Hit
CTATCGGTCAGTCAGGAGTATTTAG15490.38537610867158445No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG15340.3816442548109816No Hit
GCCCAGAGCCTGAATCAGTGTGTGT15290.380400303524114No Hit
GCCTTGGAGGATGGTCCCCCCATAT15030.37393175683240243No Hit
GTCAGGAGTATTTAGCCTTGGAGGA14950.37194143477341424No Hit
AGCGTACACGGTGGATGCCCTGGCA14390.35800918036049706No Hit
CACACACACTGATTCAGGCTCTGGG13610.3386035402853624No Hit
CGGTACTGGTTCACTATCGGTCAGT13290.3306422520494097No Hit
TCACGGTACTGGTTCACTATCGGTC13190.3281543494756745No Hit
TATTCAGACAGGATACCACGTGTCC12760.31745636840861313No Hit
GGTTAATGAGGCGAACCGGGGGAAC12710.3162124171217455No Hit
TCTAAGTACCCCGAGGAAAAGAAAT12460.3099926606874075No Hit
ATCATTAACTGAATCCATAGGTTAA12340.3070071775989252No Hit
CATCTAAGTACCCCGAGGAAAAGAA11130.27690355645672915No Hit
GTGGATGCCCTGGCAGTCAGAGGCG11000.27366928311087335No Hit
AGCTCACAGCATGTGCATTTTTGTG10810.26894226822077644No Hit
GTACTTAGATGTTTCAGTTCCCCCG10720.2667031559044148No Hit
TCATTAACCTATGGATTCAGTTAAT10540.2622249312716914No Hit
GCCTGAATCAGTGTGTGTGTTAGTG10530.2619761410143179No Hit
GTATCAACGCAGAGTACATGGGGGT10350.25749791638159447No Hit
GGTTCACTATCGGTCAGTCAGGAGT10000.24879025737352128No Hit
CCCCAGTAGCGGCGAGCGAACGGGG10000.24879025737352128No Hit
CAACGCAGAGTACTTTTTTTTTTTT9940.2472975158292801No Hit
GTACATGGGGGTTAAGCGACTAAGC9720.24182413016706267No Hit
GGGTGACAGCCCCGTACACAAAAAT9270.2306285685852542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9170.228140666011519No Hit
ACGCAGAGTACATGGGGGTTAAGCG9120.2268967147246514No Hit
GGAGTATTTAGCCTTGGAGGATGGT8810.21918421674607222No Hit
CCTATGGATTCAGTTAATGATAGTG8800.2189354264886987No Hit
GGGTTTCCCCATTCGGAAATCGCCG8720.21694510442971052No Hit
GGGTACTTAGATGTTTCAGTTCCCC8500.21147171876749307No Hit
AACGCAGAGTACTTTTTTTTTTTTT8490.21122292851011953No Hit
GTCCCGCCCTACTCATCGAGCTCAC8470.21072534799537251No Hit
GGTATCAACGCAGAGTACATGGGGA8320.20699349413476967No Hit
GTGCTAATCTGCGATAAGCGTCGGT8310.20674470387739616No Hit
GCCCTACTCATCGAGCTCACAGCAT8280.2059983331052756No Hit
GAATCAGTGTGTGTGTTAGTGGAAG8180.2035104305315404No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG8180.2035104305315404No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA7690.19131970792023786No Hit
ATGATAGTGTGTCGAAACACACTGG7590.18883180534650265No Hit
TTCCAGACGCTTCCACTAACACACA7540.18758785405963505No Hit
TCAACGCAGAGTACTTTTTTTTTTT7180.17863140479418826No Hit
CAGTAGCGGCGAGCGAACGGGGAGC7180.17863140479418826No Hit
AGTCAGGAGTATTTAGCCTTGGAGG7160.17813382427944122No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC7120.17713866324994715No Hit
GTTATAACCGGCGATTTCCGAATGG6870.17091890681560912No Hit
GATATGAACCGTTATAACCGGCGAT6810.16942616527136797No Hit
GTCCCCCCATATTCAGACAGGATAC6800.16917737501399444No Hit
GTGGTATCAACGCAGAGTACATGGG6790.16892858475662093No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6590.1639527796091505No Hit
CACTATCATTAACTGAATCCATAGG6510.16196245755016234No Hit
TCCCCCAGTAGCGGCGAGCGAACGG6490.1614648770354153No Hit
CTACTGGGGGAATCTCGGTTGATTT6430.15997213549117417No Hit
CTGTCACCCTGTATCGCGCGCCTTT6300.1567378621453184No Hit
AACACACACACTGATTCAGGCTCTG6150.15300600828471558No Hit
GGTGATATGAACCGTTATAACCGGC6130.1525084277699685No Hit
TAATGATAGTGTGTCGAAACACACT6120.152259637512595No Hit
CTAACACACACACTGATTCAGGCTC6010.14952294468148628No Hit
GTATCGCGCGCCTTTCCAGACGCTT5790.1440495590192688No Hit
CATTAACCTATGGATTCAGTTAATG5550.13807859284230428No Hit
TATCAACGCAGAGTACATGGGGGTT5470.13608827078331615No Hit
TCGGTAAGGTGATATGAACCGTTAT5430.13509310975382205No Hit
GTACGGGGCTGTCACCCTGTATCGC5430.13509310975382205No Hit
GGGCTGTCACCCTGTATCGCGCGCC5400.13434673898170146No Hit
CGTCTGGAAAGGCGCGCGATACAGG5340.13285399743746035No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5340.13285399743746035No Hit
CAGGGTGACAGCCCCGTACACAAAA5200.12937093383423104No Hit
GTGGTATCCTGTCTGAATATGGGGG5200.12937093383423104No Hit
CACTGATTCAGGCTCTGGGCTGCTC5110.12713182151786936No Hit
GGTATCAACGCAGAGTACATGGGCA5110.12713182151786936No Hit
TAAGTACCCCGAGGAAAAGAAATCA5050.12563907997362825No Hit
GTACACAAAAATGCACATGCTGTGA5040.1253902897162547No Hit
GAGTACATGGGGGTTAAGCGACTAA5010.12464391894413415No Hit
TTTGTGTACGGGGCTGTCACCCTGT4960.12339996765726655No Hit
CCGTTATAACCGGCGATTTCCGAAT4930.12265359688514597No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4910.12215601637039894No Hit
CTGTGAGCTCGATGAGTAGGGCGGG4890.12165843585565188No Hit
CCACGTGTCCCGCCCTACTCATCGA4890.12165843585565188No Hit
CTATCATTAACTGAATCCATAGGTT4860.12091206508353133No Hit
ATCCATAGGTTAATGAGGCGAACCG4650.11568746967868739No Hit
CTACTCATCGAGCTCACAGCATGTG4640.11543867942131388No Hit
CCCTGGCAGTCAGAGGCGATGAAGG4610.1146923086491933No Hit
GGAGGATGGTCCCCCCATATTCAGA4600.11444351839181978No Hit
CCGCTACTGGGGGAATCTCGGTTGA4510.11220440607545808No Hit
ATTCAGACAGGATACCACGTGTCCC4490.11170682556071104No Hit
CACTAACACACACACTGATTCAGGC4490.11170682556071104No Hit
CAGTTAATGATAGTGTGTCGAAACA4470.111209245045964No Hit
GTTATAACGGTTCATATCACCTTAC4470.111209245045964No Hit
GCGTACACGGTGGATGCCCTGGCAG4440.11046287427384344No Hit
GCATGTGCATTTTTGTGTACGGGGC4350.10822376195748175No Hit
AAAATGCACATGCTGTGAGCTCGAT4330.10772618144273471No Hit
ATTTAGCCTTGGAGGATGGTCCCCC4270.10623343989849357No Hit
AAGTACCCCGAGGAAAAGAAATCAA4220.10498948861162596No Hit
GTCTGGAAAGGCGCGCGATACAGGG4190.1042431178395054No Hit
AACGGGGAGCAGCCCAGAGCCTGAA4120.10250158603789077No Hit
GCGCGATACAGGGTGACAGCCCCGT4090.1017552152657702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT307.6119945E-419.035836
TACATAA307.695498E-419.0049862
TCCAAAA359.8963224E-519.0049862
TACGGCC250.00607622418.9718819
GTTACGG250.00608059318.96951917
TTACGGC250.00608059318.96951918
GGTATCA27350.016.3746431
CCGAGAG350.002142897816.3123519
TATAAAG350.002162634416.2899882
GTACATA601.4467732E-615.8473641
GTACAAA601.4467732E-615.8473641
GAATAAA508.622873E-515.21346951
CAACCCC508.8081404E-515.17561512
TACATGA456.7334645E-414.7816552
AACCCCG653.4135119E-614.59193813
CAGTTCG400.005326527614.22713913
AGTTCGC400.005326527614.22713914
AATCGCC1600.014.22713918
AAATCGC1650.013.79601517
ATCGCCG1600.013.63603919
Produced by FastQC (version 0.11.5)