FastQCFastQC Report
Wed 25 May 2016
SRR1294633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137218
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103840.48586526971043664No Hit
GGTATCAACGCAGAGTACTTTTTTT64850.30343184457551825No Hit
TATCAACGCAGAGTACTTTTTTTTT62430.2921087132898937No Hit
GTACATGGGAAGCAGTGGTATCAAC30020.14046297570018593No Hit
CCCATGTACTCTGCGTTGATACCAC29220.1367197918041117No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28980.13559683663528943No Hit
GTACTGGTTCACTATCGGTCAGTCA25270.11823782131724513No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21780.10190818157062125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG400.005290113414.2458738
GTACGAC400.00529093514.2455413
GGTATCA28550.011.379671
ACCGGGG1553.6379788E-910.423902515
GTTCTAA2100.010.4044281
TCCGACG750.002638183310.13799416
TGTCCCG3200.010.090590511
AATCGCC1252.5533245E-69.88523918
GAACCGG1252.556384E-69.88431213
GTACTAG1451.6183367E-79.8272711
CTTAGAC1451.624976E-79.8245113
GTCTTAG1554.0665327E-89.8061371
CTACACT3300.09.7850454
CCCGTAC4200.09.73066316
CCCCGTA3850.09.62757815
GTATCAA53900.09.5878191
CGACGCC900.00110848539.50503718
AAATCGC1106.803594E-59.50436917
AACCGGG1606.5921995E-89.50414714
TCTGTCG1106.859601E-59.497258
Produced by FastQC (version 0.11.5)