##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294633_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2137218 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.386644694177196 31.0 30.0 33.0 23.0 34.0 2 29.39881472081931 31.0 30.0 34.0 23.0 34.0 3 29.342857396858907 31.0 28.0 34.0 22.0 34.0 4 32.89089086840931 35.0 33.0 37.0 25.0 37.0 5 33.34814745150003 35.0 33.0 37.0 27.0 37.0 6 33.450868371874094 35.0 33.0 37.0 27.0 37.0 7 33.32376528739698 35.0 33.0 37.0 26.0 37.0 8 33.13580317964756 35.0 33.0 37.0 25.0 37.0 9 34.11379653362455 37.0 33.0 39.0 23.0 39.0 10 34.26400348490421 37.0 33.0 39.0 25.0 39.0 11 34.5033244151977 38.0 34.0 39.0 25.0 39.0 12 34.27851019409344 37.0 33.0 39.0 25.0 39.0 13 34.079068677130735 37.0 33.0 39.0 23.0 39.0 14 34.837041892778366 38.0 33.0 40.0 22.0 41.0 15 34.849116936129114 38.0 33.0 40.0 22.0 41.0 16 34.959170753755586 38.0 33.0 40.0 24.0 41.0 17 34.961671668496145 38.0 33.0 40.0 24.0 41.0 18 34.86558928476178 38.0 33.0 40.0 23.0 41.0 19 35.17237829739409 39.0 33.0 40.0 24.0 41.0 20 35.30314127992558 39.0 34.0 40.0 25.0 41.0 21 35.35007472330852 39.0 34.0 40.0 25.0 41.0 22 35.37099678179764 39.0 34.0 40.0 25.0 41.0 23 35.357502603852296 39.0 34.0 40.0 25.0 41.0 24 35.38473754198215 39.0 34.0 41.0 25.0 41.0 25 34.05773440051506 38.0 31.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0060051815750342 1101 2 0.9583680939791037 1101 3 1.2999376869376391 1101 4 1.0546120366393126 1101 5 0.7436590185417415 1101 6 0.7388248085757851 1101 7 0.7422713533399232 1101 8 0.9331821406001453 1101 9 1.2428199404754992 1101 10 1.107517893071666 1101 11 0.7933827990771078 1101 12 1.0987698095867344 1101 13 1.1955558773605475 1101 14 1.2717006666533806 1101 15 1.6234613126859685 1101 16 1.429368047294247 1101 17 1.3850564312905718 1101 18 1.3262823349088109 1101 19 1.2465414438388578 1101 20 1.121378183695299 1101 21 1.0840287110367228 1101 22 1.1087887743770892 1101 23 1.0346486592739197 1101 24 1.0841852206210518 1101 25 1.078147654749337 1102 1 1.0818333911740048 1102 2 0.9780865911588883 1102 3 1.284337461905622 1102 4 1.133679536935233 1102 5 1.0286349393177048 1102 6 0.9196089095050439 1102 7 0.8471102067406022 1102 8 0.8969041063585763 1102 9 1.262640180855449 1102 10 1.2436991050260247 1102 11 1.0174862286874387 1102 12 1.255611009065916 1102 13 1.2541369520613372 1102 14 1.4386967684923349 1102 15 1.5316353480872849 1102 16 1.3934814251285914 1102 17 1.2962363465178655 1102 18 1.4953323194145867 1102 19 1.1797016142354408 1102 20 0.42881315117072205 1102 21 0.8715326510920249 1102 22 0.8369252246432808 1102 23 0.976823676197867 1102 24 1.1216348311374418 1102 25 1.0668121322747268 1103 1 0.6280578173402311 1103 2 0.7728853894614325 1103 3 1.0133098866413768 1103 4 0.7438545886735497 1103 5 0.5677917112598507 1103 6 0.45907514307175035 1103 7 0.4699921633333517 1103 8 0.6393033183094445 1103 9 0.5264269738027707 1103 10 0.6931215595201934 1103 11 0.5634024842663337 1103 12 0.7677221002376768 1103 13 0.5525332256114694 1103 14 0.4810597022969887 1103 15 0.8347632730935146 1103 16 0.8312770565168037 1103 17 0.7346382489903363 1103 18 0.7629869308059227 1103 19 0.6493845742223243 1103 20 -0.26185548992221896 1103 21 0.33368279614005303 1103 22 -0.3703506738863567 1103 23 0.17506425065779752 1103 24 0.35077108849797156 1103 25 0.4740913875011685 1104 1 0.37586063023026384 1104 2 0.3990235883198494 1104 3 0.6061186897553412 1104 4 0.4628798001708816 1104 5 0.35496332197813274 1104 6 0.25053301116268756 1104 7 0.20977005328791876 1104 8 0.5012471140667145 1104 9 0.6438388667060266 1104 10 0.6074717434815113 1104 11 0.35630866762399194 1104 12 0.5189592497870521 1104 13 0.4758986527969 1104 14 0.7605590211858768 1104 15 0.7832614875387733 1104 16 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0.5196455313438619 2207 7 0.42432316512694257 2207 8 0.3879428860135832 2207 9 -1.1765589497947602 2207 10 -0.16826103700925898 2207 11 0.1441688286465066 2207 12 0.49423735500825217 2207 13 0.7737256864177695 2207 14 1.078119699268683 2207 15 0.8924097683312624 2207 16 0.9140298670847713 2207 17 1.0507142040684911 2207 18 0.9857705487845863 2207 19 -0.17706319337424503 2207 20 0.31042192700796534 2207 21 -0.21895117605768633 2207 22 0.35480314466644813 2207 23 -0.2737602985163079 2207 24 -1.5966934417598821 2207 25 -0.015199364110358715 2208 1 0.5344634036205669 2208 2 0.17848316353128624 2208 3 0.6321219034691339 2208 4 0.5504623950326746 2208 5 0.3321207998832776 2208 6 0.36796697518241217 2208 7 0.4257314148334572 2208 8 0.5225362575431163 2208 9 -0.08905116325549045 2208 10 0.18899646960203142 2208 11 0.3908367064330349 2208 12 0.4263307685108302 2208 13 0.6431830372767493 2208 14 0.8154251111233393 2208 15 0.7067329324192073 2208 16 0.49670870780199294 2208 17 0.5314964655248744 2208 18 0.6438446142169099 2208 19 -1.4194635956267518 2208 20 -0.07818374542400619 2208 21 -0.2951884447700266 2208 22 0.23822611378418657 2208 23 0.46795120756720365 2208 24 0.5331015489393209 2208 25 0.7268525541872606 2209 1 0.3000859663192621 2209 2 -1.3265672173819958 2209 3 -0.18499939271070787 2209 4 0.2373028140830442 2209 5 0.31619993544559577 2209 6 0.37784141712354113 2209 7 0.24874761516624488 2209 8 0.5885680368132356 2209 9 -1.8123170602855865 2209 10 -0.2660490273983527 2209 11 0.0961043735889433 2209 12 0.46387294945139956 2209 13 0.5330414378516721 2209 14 0.7004083770714402 2209 15 0.8973493396851921 2209 16 0.7372847775651223 2209 17 0.48018830040988547 2209 18 0.6916429590492541 2209 19 0.45683753090787604 2209 20 0.40697894846148586 2209 21 0.4525841610965031 2209 22 0.33698840895511495 2209 23 0.49322183911991857 2209 24 0.5123714667384931 2209 25 0.4363315587883534 2210 1 0.13313131925211863 2210 2 0.058245773296803804 2210 3 0.12231268720080735 2210 4 0.20518402443110517 2210 5 0.14557053359883554 2210 6 -0.07044370251553289 2210 7 0.060647246270377764 2210 8 0.04782076123269263 2210 9 0.23093525456175001 2210 10 -1.5643742457717806 2210 11 -0.6621859499886469 2210 12 -2.568018265445037 2210 13 -1.0257694668812931 2210 14 -0.7594619833613905 2210 15 -0.96467870414277 2210 16 -0.16258966610200787 2210 17 -0.004689684869866539 2210 18 0.35695338631227 2210 19 0.2502307142166913 2210 20 0.12737526568383828 2210 21 0.20898739359454765 2210 22 0.21042547142391754 2210 23 -0.03887743966478041 2210 24 0.08039243733077939 2210 25 -0.04345440231540465 2211 1 -0.3520716473201979 2211 2 0.005339511727289192 2211 3 -1.4802155871951896 2211 4 -0.5687979579812392 2211 5 -0.4487510998471578 2211 6 -0.40578142280900664 2211 7 -0.42437060810006244 2211 8 -0.056787537367220864 2211 9 0.09528383875197477 2211 10 0.12365198044727066 2211 11 -0.34594862420311046 2211 12 -0.17259869769198133 2211 13 -0.21586377458156392 2211 14 -0.585149983711716 2211 15 -0.14604885290928848 2211 16 0.019676186867151557 2211 17 -0.061381789535886355 2211 18 0.10152641407113805 2211 19 -0.033944382021751096 2211 20 0.12195924093851573 2211 21 -0.09855563006398427 2211 22 -0.08448215161598682 2211 23 -0.43919153327183835 2211 24 -0.20598808169994953 2211 25 -0.03066772720921307 2212 1 -0.295728187950953 2212 2 -0.25874034179236105 2212 3 -2.234131629945935 2212 4 -1.315592809795458 2212 5 -0.6303308932535856 2212 6 -0.5806113914343527 2212 7 -0.3516544208994645 2212 8 -0.35476123181926056 2212 9 -0.833661770800596 2212 10 -0.16470827322163473 2212 11 -0.44270030581496655 2212 12 0.14045462229473316 2212 13 -0.24901877943069906 2212 14 0.14261780735430563 2212 15 0.24270516465802672 2212 16 -1.0817645490612335 2212 17 -1.1997564258508646 2212 18 -0.18195186635216487 2212 19 -0.20084062926433432 2212 20 -0.14711524246622787 2212 21 -0.016490166314930832 2212 22 -0.2992815159297493 2212 23 0.025744421651261007 2212 24 -0.10914556232345518 2212 25 0.28711783956055115 2213 1 -3.270918794037428 2213 2 -1.5049873927563127 2213 3 -2.2787476149747903 2213 4 -1.1669079793703965 2213 5 -0.9661544484122473 2213 6 -0.7275772776990195 2213 7 -0.3079478772577957 2213 8 -0.2237205919230476 2213 9 -0.20696304021296896 2213 10 -0.1155496547188335 2213 11 -0.3444310054665678 2213 12 -0.3634267789146506 2213 13 -0.5526718689002621 2213 14 -0.2313501711845305 2213 15 -0.3764327298510679 2213 16 -1.7383379238786887 2213 17 -1.0913487765832315 2213 18 -0.492439740692852 2213 19 -0.28765425425129365 2213 20 -0.24684703432831157 2213 21 -0.02165750081997686 2213 22 0.0361867207556088 2213 23 0.13393909439238172 2213 24 0.1548746959676066 2213 25 0.08446124535107202 2214 1 -0.8679682958825836 2214 2 -0.6474072481456936 2214 3 -6.252631082479805 2214 4 -2.1565393723800064 2214 5 -1.0802754203172924 2214 6 -0.7167674891901967 2214 7 -0.3761748659903361 2214 8 -0.2599715114718606 2214 9 -0.1364611566943097 2214 10 0.11681233892357312 2214 11 -0.6374196182047669 2214 12 -0.35794179221066713 2214 13 -4.95172038406028 2214 14 -3.859926820668335 2214 15 -2.300886031578166 2214 16 -2.6810082196082803 2214 17 -3.1674827073120113 2214 18 -0.7550824011621131 2214 19 -0.3298912430982526 2214 20 -0.07967726593009417 2214 21 -0.23645670097235438 2214 22 -0.01739479148406531 2214 23 -0.0460249380403468 2214 24 0.0988109908370518 2214 25 0.24104602253589746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 793.0 3 94.0 4 129.0 5 219.0 6 45.0 7 66.0 8 152.0 9 422.0 10 941.0 11 2024.0 12 3953.0 13 7095.0 14 11127.0 15 15636.0 16 19669.0 17 21984.0 18 23181.0 19 22811.0 20 22696.0 21 22288.0 22 21627.0 23 22001.0 24 22561.0 25 23967.0 26 25971.0 27 29152.0 28 33599.0 29 40201.0 30 49745.0 31 61327.0 32 78138.0 33 101687.0 34 133761.0 35 178611.0 36 240563.0 37 344545.0 38 554437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63390891701965 21.135652154364088 13.349910168478596 22.88052876013766 2 15.24799752821718 22.29515333948158 39.93380490705067 22.523044225250572 3 18.30711287864779 28.142861489989723 29.386228350861753 24.163797280500738 4 12.064885472955101 19.041042856413902 37.35699041211385 31.53708125851714 5 12.164040973729962 40.361614419637135 34.23254773199862 13.241796874634279 6 28.94679794606333 39.67293769410165 17.931071293627074 13.449193066207949 7 25.218163609949357 35.27431332128843 22.142921685830355 17.364601382931856 8 24.18534098330723 37.878232453399065 20.576603907853862 17.35982265543984 9 24.870811528803983 18.50664354376021 21.2739479163399 35.348597011095904 10 14.989868135943022 31.75159515992801 32.36545315277016 20.893083551358814 11 30.95414774278091 27.533967019184267 23.14527973018335 18.36660550785147 12 20.668942324663767 29.33042563481448 30.50171376617029 19.498918274351468 13 26.33339544499887 25.547464392693048 26.278993116841402 21.840147045466676 14 20.33854977468251 25.35761690173816 27.858605957745652 26.445227365833674 15 22.44046782968602 31.94229850830126 24.438295361968706 21.178938300044013 16 20.73829892922872 31.759504142678363 26.165753016123606 21.33644391196931 17 19.720123284714557 31.091794487395997 27.728245505693465 21.459836722195977 18 20.17990486043949 29.92889836036077 29.308551314635302 20.58264546456445 19 20.908175338530192 29.891884780105787 29.51675198300197 19.683187898362046 20 22.080891730480754 28.616849541081663 29.223434518224707 20.078824210212876 21 22.356735073770867 28.433315948309364 28.85107151986621 20.358877458053556 22 21.875159479011042 28.590671396687334 28.744660818283585 20.789508306018043 23 21.535179839071397 28.57709375266287 28.908202526951747 20.979523881313984 24 21.43374220603963 28.498923969904872 28.863125975123012 21.20420784893249 25 21.400682380362205 28.576679297617382 29.01839580214809 21.004242519872328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3530.0 1 3530.0 2 3861.5 3 4193.0 4 4193.0 5 4193.0 6 6557.0 7 8921.0 8 8921.0 9 8921.0 10 12495.5 11 16070.0 12 16070.0 13 16070.0 14 23445.5 15 30821.0 16 30821.0 17 30821.0 18 44811.0 19 58801.0 20 58801.0 21 58801.0 22 78901.0 23 99001.0 24 99001.0 25 99001.0 26 122861.0 27 146721.0 28 146721.0 29 146721.0 30 167830.0 31 188939.0 32 188939.0 33 188939.0 34 201154.0 35 213369.0 36 213369.0 37 213369.0 38 213734.0 39 214099.0 40 214099.0 41 214099.0 42 215734.5 43 217370.0 44 217370.0 45 217370.0 46 224265.5 47 231161.0 48 231161.0 49 231161.0 50 219859.5 51 208558.0 52 208558.0 53 208558.0 54 190453.5 55 172349.0 56 172349.0 57 172349.0 58 152639.0 59 132929.0 60 132929.0 61 132929.0 62 114193.0 63 95457.0 64 95457.0 65 95457.0 66 73609.5 67 51762.0 68 51762.0 69 51762.0 70 38417.5 71 25073.0 72 25073.0 73 25073.0 74 18294.0 75 11515.0 76 11515.0 77 11515.0 78 7888.0 79 4261.0 80 4261.0 81 4261.0 82 2947.0 83 1633.0 84 1633.0 85 1633.0 86 1045.0 87 457.0 88 457.0 89 457.0 90 311.5 91 166.0 92 166.0 93 166.0 94 102.5 95 39.0 96 39.0 97 39.0 98 31.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09872647525895814 2 0.051843096960628256 3 0.0544165358891793 4 0.05076693159050691 5 0.04772559467494659 6 0.06630114475921502 7 0.06180932408392592 8 0.0590955157592721 9 0.06480387120078532 10 0.06466350180468253 11 0.05844045857745911 12 0.059844152538486954 13 0.05989094233718788 14 0.06283869965534634 15 0.06541213858389738 16 0.05993773213588881 17 0.06311943844755191 18 0.1445804779858676 19 0.06742409992803729 20 0.062043273077430565 21 0.05989094233718788 22 0.06349375683715933 23 0.06382128542806584 24 0.07439577993447558 25 0.06826631630465399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2137218.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.7633299066058 #Duplication Level Percentage of deduplicated Percentage of total 1 86.73216601294118 63.10921209114143 2 9.40364739570667 13.68481395558398 3 2.0813964885710448 4.543480180930374 4 0.6874489385370338 2.000842956348647 5 0.3095348527956243 1.1261393305780338 6 0.17609321714942047 0.7687877312255316 7 0.10875074952946863 0.5539146665922368 8 0.07453349859150547 0.43386444376858024 9 0.056423304377890705 0.3694992759782368 >10 0.30753445342824154 4.127759212175279 >50 0.03187334712017857 1.6173597609777082 >100 0.02658120518453144 3.695812562892499 >500 0.0024617479121983306 1.2567973937646966 >1k 0.0013604396356883658 1.622259481899622 >5k 1.2956567958936818E-4 0.5999743915623533 >10k+ 6.478283979468409E-5 0.48948256458072575 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10384 0.48586526971043664 No Hit GGTATCAACGCAGAGTACTTTTTTT 6485 0.30343184457551825 No Hit TATCAACGCAGAGTACTTTTTTTTT 6243 0.2921087132898937 No Hit GTACATGGGAAGCAGTGGTATCAAC 3002 0.14046297570018593 No Hit CCCATGTACTCTGCGTTGATACCAC 2922 0.1367197918041117 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2898 0.13559683663528943 No Hit GTACTGGTTCACTATCGGTCAGTCA 2527 0.11823782131724513 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2178 0.10190818157062125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGG 40 0.0052901134 14.245873 8 GTACGAC 40 0.005290935 14.245541 3 GGTATCA 2855 0.0 11.37967 1 ACCGGGG 155 3.6379788E-9 10.4239025 15 GTTCTAA 210 0.0 10.404428 1 TCCGACG 75 0.0026381833 10.137994 16 TGTCCCG 320 0.0 10.0905905 11 AATCGCC 125 2.5533245E-6 9.885239 18 GAACCGG 125 2.556384E-6 9.884312 13 GTACTAG 145 1.6183367E-7 9.827271 1 CTTAGAC 145 1.624976E-7 9.824511 3 GTCTTAG 155 4.0665327E-8 9.806137 1 CTACACT 330 0.0 9.785045 4 CCCGTAC 420 0.0 9.730663 16 CCCCGTA 385 0.0 9.627578 15 GTATCAA 5390 0.0 9.587819 1 CGACGCC 90 0.0011084853 9.505037 18 AAATCGC 110 6.803594E-5 9.504369 17 AACCGGG 160 6.5921995E-8 9.504147 14 TCTGTCG 110 6.859601E-5 9.49725 8 >>END_MODULE