FastQCFastQC Report
Wed 25 May 2016
SRR1294633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137218
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT282781.323121927664843No Hit
GGTATCAACGCAGAGTACTTTTTTT194810.9115120684927789No Hit
TATCAACGCAGAGTACTTTTTTTTT180080.8425906950063119No Hit
ACGCAGAGTACTTTTTTTTTTTTTT96110.4496967553146193No Hit
GTACTTTTTTTTTTTTTTTTTTTTT60900.2849498740886517No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50560.2365692222318921No Hit
CCCATGTACTCTGCGTTGATACCAC35120.16432577303765922No Hit
GCAGAGTACTTTTTTTTTTTTTTTT34150.15978716256366923No Hit
GTACATGGGAAGCAGTGGTATCAAC29950.14013544710927944No Hit
ATCAACGCAGAGTACTTTTTTTTTT28250.13218118133012166No Hit
GTACTGGTTCACTATCGGTCAGTCA25970.12151310722631008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG655.540027E-513.13201119
GGTATCA43000.012.4329031
CATACCG550.003028355312.1124975
AAATCGC951.3860288E-510.98174217
GTACTAG1408.183633E-910.8716791
AATCGCC909.679635E-510.538033518
CTACACT3800.010.5187484
TGTCCCG3900.010.46284811
CTAGGGC1303.8266808E-710.23393
ACACCGT951.6151342E-410.0178566
ACCGTGT1054.026448E-59.9701518
CGCAAAA1151.02298745E-59.9160782
GTCCAAG1951.6007107E-109.7566351
GTATCAA83600.09.7402961
CCCCGGT2054.1836756E-119.71555319
ACTGAGC3800.09.5169638
GGACTAG1002.7191307E-49.5127191
GTATGGG1106.7385285E-59.5127191
ATAGGAC1106.815045E-59.5029073
TGACGAA900.00113068339.48445212
Produced by FastQC (version 0.11.5)