##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2137218 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.241365176598737 31.0 31.0 34.0 28.0 34.0 2 31.30814872418256 31.0 31.0 34.0 28.0 34.0 3 31.463521737136784 31.0 31.0 34.0 28.0 34.0 4 35.04446153831757 37.0 35.0 37.0 32.0 37.0 5 35.2633914743372 37.0 35.0 37.0 32.0 37.0 6 34.80906767582905 37.0 35.0 37.0 32.0 37.0 7 34.62653178103497 37.0 35.0 37.0 31.0 37.0 8 34.66201295328787 37.0 35.0 37.0 31.0 37.0 9 36.02346601984449 39.0 35.0 39.0 30.0 39.0 10 35.828972991992394 39.0 35.0 39.0 30.0 39.0 11 36.00273158844816 39.0 35.0 39.0 30.0 39.0 12 35.95703713893482 39.0 35.0 39.0 30.0 39.0 13 35.80798028090724 39.0 35.0 39.0 30.0 39.0 14 36.82591574654528 40.0 36.0 41.0 30.0 41.0 15 36.8829024460771 40.0 36.0 41.0 30.0 41.0 16 36.93917092219886 40.0 36.0 41.0 30.0 41.0 17 37.0291598704484 40.0 36.0 41.0 30.0 41.0 18 37.04761095966813 40.0 36.0 41.0 30.0 41.0 19 37.06906501816848 40.0 36.0 41.0 30.0 41.0 20 37.13689197826333 40.0 37.0 41.0 30.0 41.0 21 36.97306264498989 40.0 36.0 41.0 30.0 41.0 22 36.99323559880181 40.0 36.0 41.0 30.0 41.0 23 37.00007813896383 40.0 36.0 41.0 30.0 41.0 24 36.92075258583822 40.0 36.0 41.0 30.0 41.0 25 35.82310227594939 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5600269914578817 1101 2 0.515358495804481 1101 3 0.4023041110350398 1101 4 0.34159596834001604 1101 5 0.3872406656242049 1101 6 0.4972663967362081 1101 7 0.46517496576678496 1101 8 0.4163501769928857 1101 9 0.6703649642248735 1101 10 0.7062571029303228 1101 11 0.6477429833274968 1101 12 0.7587395207661061 1101 13 0.8218698343938087 1101 14 0.9247862969905611 1101 15 0.9600046381239338 1101 16 0.947170872822106 1101 17 0.8492189557520149 1101 18 0.9737565386033324 1101 19 1.0396824641927722 1101 20 0.997421949164945 1101 21 1.301669234038684 1101 22 1.140671040138514 1101 23 1.097446599507144 1101 24 1.2056143437543838 1101 25 1.1464574916294907 1102 1 0.36156018833563053 1102 2 0.40479502467228556 1102 3 0.36944863824835394 1102 4 0.2328715886742927 1102 5 0.3185016296177494 1102 6 0.4537016873959203 1102 7 0.41498950054766937 1102 8 0.45772441508260897 1102 9 0.6088857377923844 1102 10 0.7918752693449633 1102 11 0.7233941730579474 1102 12 0.6932881866002063 1102 13 0.821339040783549 1102 14 0.9087517016425579 1102 15 0.903615098654889 1102 16 0.9747887652615859 1102 17 0.9175184345342302 1102 18 0.7978059711688701 1102 19 0.9410862543597247 1102 20 0.8259272019070281 1102 21 1.0826525654435528 1102 22 0.9770404437637623 1102 23 0.9758695932043864 1102 24 1.1963944148549288 1102 25 0.933793854649231 1103 1 0.24260285582511543 1103 2 0.18169191340165725 1103 3 0.1280189952588806 1103 4 0.08447744533559387 1103 5 0.08035147940556442 1103 6 0.06913978151511202 1103 7 0.1647028257302523 1103 8 0.1925589234749978 1103 9 -0.07391080377707482 1103 10 0.1796487594446461 1103 11 0.3176396328027664 1103 12 0.29947767729893116 1103 13 0.5098559101215443 1103 14 0.524780574399216 1103 15 0.5135285862499543 1103 16 0.45027209732540285 1103 17 0.5221662976739552 1103 18 0.4745289571830682 1103 19 0.5201559587509266 1103 20 0.4289150493735576 1103 21 0.6519137435666735 1103 22 0.5467621348676133 1103 23 0.6268096619650265 1103 24 0.7679337572780227 1103 25 0.7126341417242728 1104 1 0.1272331700372007 1104 2 0.06253175606161321 1104 3 -0.04687552199063916 1104 4 -0.03466331604226269 1104 5 0.02512842208636812 1104 6 0.03324361172961687 1104 7 0.08680388645162651 1104 8 0.09671308808840706 1104 9 -0.29926873436055246 1104 10 0.25789707631309966 1104 11 0.28629062736514044 1104 12 0.2622101504192571 1104 13 -0.22743829559128415 1104 14 0.14510611622854697 1104 15 0.3097551080634062 1104 16 0.23160226990942334 1104 17 0.23507039377582117 1104 18 0.2757143382836915 1104 19 0.4290443648958657 1104 20 0.28016566347474736 1104 21 0.49586709615248026 1104 22 0.5707126443807695 1104 23 0.46852359429739465 1104 24 0.550644922369699 1104 25 0.47091527906344766 1105 1 0.06042794328490331 1105 2 0.08905753137167594 1105 3 0.0338684676859593 1105 4 0.015861306300479328 1105 5 0.03344066992104189 1105 6 0.020092824975023404 1105 7 0.01070940038459156 1105 8 -0.11211157869984589 1105 9 0.2644649153807066 1105 10 0.295698092217485 1105 11 0.17908996144753786 1105 12 -0.2340481349909922 1105 13 -0.34927007242695396 1105 14 -0.6436074392566482 1105 15 -0.0598400182805392 1105 16 0.12540625509951298 1105 17 0.2611183983797929 1105 18 -0.49585783581733267 1105 19 -0.44325339878679415 1105 20 -0.3628383149041525 1105 21 0.174051755023946 1105 22 0.31948804846842194 1105 23 0.40556410091203077 1105 24 0.5945804549125242 1105 25 0.37281175237035313 1106 1 -0.4795056192583118 1106 2 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24 0.3721328907521908 1108 25 0.2944264530419787 1109 1 0.019033053738322536 1109 2 -0.058097091751417196 1109 3 -0.09921356035347273 1109 4 -0.11597428106166774 1109 5 -0.010384487449385915 1109 6 -0.06380242690218552 1109 7 -0.3079651376307453 1109 8 -0.20509185567085808 1109 9 -0.10549127030560612 1109 10 -0.3965866032283216 1109 11 -0.07976282807853607 1109 12 0.11871871262528089 1109 13 0.17955424718142154 1109 14 0.10327841207924138 1109 15 -3.542569702376042 1109 16 -1.8095891235088786 1109 17 -1.4441915438443615 1109 18 -0.8835355745850606 1109 19 -0.42651728007247414 1109 20 -0.42877310285357595 1109 21 0.14200347987514306 1109 22 0.23121276440762273 1109 23 0.13904424373617275 1109 24 0.19031100498696674 1109 25 0.16418789866727934 1110 1 -0.18909307264233277 1110 2 -0.14215088037584778 1110 3 -0.2026455071482829 1110 4 -0.09473769232052831 1110 5 -0.14419024043742468 1110 6 -0.9508053890560291 1110 7 -1.0131745242312178 1110 8 -0.9412884928067555 1110 9 -0.3139934171338794 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2207 1 0.1418216156059735 2207 2 0.23176234147632258 2207 3 0.19682118886368372 2207 4 0.19085868007450557 2207 5 0.19944307416977125 2207 6 0.31274710206643874 2207 7 0.2623337589973218 2207 8 0.23683069860027217 2207 9 0.32866129139849676 2207 10 0.5580217303708253 2207 11 0.44492051385407905 2207 12 0.4607330473953368 2207 13 0.5050842102313595 2207 14 0.46933774680709917 2207 15 0.43528114099499504 2207 16 0.4785451366836284 2207 17 0.5860499825728738 2207 18 0.5514876029231175 2207 19 0.6551525111649781 2207 20 0.5199526959727336 2207 21 0.9801466024268279 2207 22 0.785571417342183 2207 23 0.703799370230044 2207 24 0.836327059394506 2207 25 0.8136152214576384 2208 1 0.10689261033286712 2208 2 0.09990098513974743 2208 3 0.02576109163257101 2208 4 0.025098487833624006 2208 5 -0.011744337014171435 2208 6 0.14909740097913726 2208 7 0.16780032929099065 2208 8 0.099387572085746 2208 9 0.21695012109940848 2208 10 0.2327457521727041 2208 11 -0.07657829863141785 2208 12 -0.3099026269437175 2208 13 -0.06608099182691518 2208 14 -0.4359363931865943 2208 15 0.07764307871215692 2208 16 0.19314226641565568 2208 17 0.12057066538617534 2208 18 0.25601528985495037 2208 19 0.32681658985218576 2208 20 0.31531480287357283 2208 21 0.5464502488303395 2208 22 0.5342018043681591 2208 23 0.4769609613143686 2208 24 0.6137579556074186 2208 25 0.6513791512373714 2209 1 0.002845634113374018 2209 2 0.04522919791917346 2209 3 0.14023293422544114 2209 4 -0.6049023357281627 2209 5 -0.026791523699458253 2209 6 0.18205115106878367 2209 7 0.23872485293923518 2209 8 0.242280702069543 2209 9 0.27390612183231156 2209 10 0.2798781185551249 2209 11 0.27909984065522764 2209 12 0.30828801207425727 2209 13 0.36747991695116866 2209 14 0.5152159655305297 2209 15 -0.1999806093081702 2209 16 -0.15230322411539987 2209 17 0.14482642196600182 2209 18 -0.15803896222433167 2209 19 0.25292112559210267 2209 20 0.38710103389946937 2209 21 0.6833904848456953 2209 22 0.56472003540852 2209 23 0.5199012492545521 2209 24 0.6399488163699303 2209 25 0.7126601813748366 2210 1 -0.1920644957103761 2210 2 -0.13038312333397073 2210 3 -0.09783273654412739 2210 4 -0.07676953821006549 2210 5 -0.04398184643417835 2210 6 -0.5258822485078483 2210 7 -0.2822352815202933 2210 8 -0.4209475254415338 2210 9 -0.1493264199217208 2210 10 0.04404745664973575 2210 11 -0.002709350555335277 2210 12 -0.009974034793657438 2210 13 0.12088425111083012 2210 14 -0.01412354010467709 2210 15 0.05280624492505126 2210 16 0.19441409588436187 2210 17 -0.9588719643760228 2210 18 -1.38199874423956 2210 19 -0.2569701036643295 2210 20 -0.16434803040616686 2210 21 -0.35801469717821277 2210 22 -0.29369896624069014 2210 23 -0.12667218980583073 2210 24 0.1789683656345602 2210 25 0.135194234081645 2211 1 -0.26648358177361686 2211 2 -0.1847165402663613 2211 3 -0.13245468965699914 2211 4 -0.08918797936627954 2211 5 -0.059985094854752674 2211 6 -0.14557619633406915 2211 7 -0.12694803913478125 2211 8 -0.1117325206047255 2211 9 0.06470906588833003 2211 10 -0.013498552689469534 2211 11 -0.37028844123642557 2211 12 -0.21224394828853832 2211 13 -0.16241703591659018 2211 14 -0.1927886621792041 2211 15 -0.19285556105347723 2211 16 -0.3427914662396958 2211 17 -0.29648278289042196 2211 18 -0.38226745550034735 2211 19 -0.13441587375099573 2211 20 -0.3430091715731294 2211 21 0.01697286248564467 2211 22 -0.060206794695425 2211 23 -0.7114874159810967 2211 24 -0.07835152815482616 2211 25 -0.7349127329046823 2212 1 -0.1632258679107963 2212 2 -0.24466657059014452 2212 3 -0.11338488172543393 2212 4 0.011447016684059008 2212 5 -0.0064769760068941196 2212 6 -0.08109106834633195 2212 7 -0.14801705713401248 2212 8 -0.15829667121374058 2212 9 -0.020801219707379914 2212 10 -0.33610197192979996 2212 11 -0.05955304273916795 2212 12 -0.21542983797765203 2212 13 -0.07985207578278875 2212 14 -0.0827013065288753 2212 15 0.03398704292681742 2212 16 -0.19803536563775737 2212 17 -0.4684001543661225 2212 18 -0.1641077618195439 2212 19 -0.36890821977875987 2212 20 -0.49462374604718207 2212 21 0.07130486650031287 2212 22 -0.32724588877573524 2212 23 -0.05167707098460994 2212 24 -0.32647543888089814 2212 25 0.09557164166394472 2213 1 -0.23358869363727663 2213 2 -0.22388645315719202 2213 3 -0.19593412040775604 2213 4 -0.01933108390861804 2213 5 -0.0877248106702595 2213 6 -0.2794611429536147 2213 7 -0.2772289897819391 2213 8 -0.31092563267876727 2213 9 -0.32757724970748825 2213 10 -0.35703340884606405 2213 11 0.005855989633715808 2213 12 0.027922889093048298 2213 13 0.0670410303274025 2213 14 -0.5725710317462429 2213 15 -0.23616961564989936 2213 16 -0.2596949496492087 2213 17 -0.20327882098400352 2213 18 -0.3004694835913426 2213 19 -0.3305588940086892 2213 20 -0.38538762260842674 2213 21 -0.09437986093715978 2213 22 -0.5184134947524512 2213 23 -0.5576948435708502 2213 24 -0.8589502374114275 2213 25 -0.5728064596514457 2214 1 -0.3267173672053545 2214 2 -0.1191120682032647 2214 3 -0.059346206829527404 2214 4 0.02846862843161091 2214 5 -0.03848377076066356 2214 6 0.04834177308459431 2214 7 -0.04904189920410573 2214 8 -0.06137337639369633 2214 9 0.011917144830611903 2214 10 -0.03907345103257143 2214 11 0.21423971727092805 2214 12 -0.07842507692890877 2214 13 -0.03236211266185052 2214 14 -0.08312438980728132 2214 15 0.07637299656197172 2214 16 -0.09855836375476912 2214 17 -0.21880598746793822 2214 18 -0.09742334562907473 2214 19 -0.325246207028151 2214 20 -0.3950922699223369 2214 21 -0.3051831882063496 2214 22 -0.750589054709728 2214 23 -0.8294512171462642 2214 24 -0.2904173493651925 2214 25 -0.5310725725360328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 30.0 10 55.0 11 149.0 12 364.0 13 913.0 14 1990.0 15 3600.0 16 5538.0 17 7371.0 18 8985.0 19 9851.0 20 10868.0 21 11496.0 22 12128.0 23 13045.0 24 14166.0 25 15492.0 26 17504.0 27 19572.0 28 23016.0 29 29066.0 30 36620.0 31 47869.0 32 64437.0 33 86682.0 34 119547.0 35 167549.0 36 237427.0 37 376114.0 38 795762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92137038421226 21.256204261281944 12.72947219638577 24.092953158120032 2 15.226242713658596 21.787014707905325 39.343529766266236 23.64321281216984 3 17.650451196647907 27.62512496802599 30.468399369233552 24.256024466092555 4 11.978835691630394 18.42078171487613 37.920789669975676 31.679592923517802 5 12.12231121527781 39.25047712737031 34.89586502092434 13.73134663642754 6 29.3018554481855 39.41790870165423 17.442242448349283 13.83799340181099 7 25.254929526710214 34.989776611113896 22.401927772190415 17.353366089985478 8 24.682880532830904 36.99860493173712 20.490375152154165 17.828139383277808 9 25.28767578367174 16.435922450478778 21.865511892813778 36.4108898730357 10 15.18756431517925 30.615833545858163 32.84985696257168 21.34674517639091 11 31.602152777940347 25.838157731483324 23.51298900435191 19.04670048622442 12 21.38382966378671 28.065796830878 31.30870748203829 19.241666023297 13 27.42008536330875 23.667028819708612 27.16086987850561 21.75201593847703 14 20.892879786000073 23.735762309230267 29.159595488857605 26.211762415912055 15 22.846235333531727 30.246894517468746 25.786781509160633 21.12008863983889 16 20.45788561281855 30.51340178690492 27.483617698734104 21.545094901542427 17 19.43982264778104 29.835870590525243 28.98537162364497 21.738935138048753 18 19.80565252809396 28.079363541975187 31.30053626164665 20.8144476682842 19 20.515617118799028 28.15503044629296 32.06302995593312 19.26632247897489 20 21.45946282327763 27.031163511698193 31.83242085128703 19.676952813737156 21 21.613027445582116 27.018249484959604 31.350484346187624 20.018238723270652 22 21.12502731432459 27.265568229194205 31.259238324559213 20.350166131921988 23 20.820516803651856 27.23817081490202 31.499372547625086 20.441939833821042 24 20.7170255912125 27.25023839402438 31.3451879967322 20.687548018030917 25 20.738462891797514 27.24103709159173 31.466112413197084 20.554387603413673 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1927.0 1 1927.0 2 2267.0 3 2607.0 4 2607.0 5 2607.0 6 6156.5 7 9706.0 8 9706.0 9 9706.0 10 12130.0 11 14554.0 12 14554.0 13 14554.0 14 19405.5 15 24257.0 16 24257.0 17 24257.0 18 38660.5 19 53064.0 20 53064.0 21 53064.0 22 75258.0 23 97452.0 24 97452.0 25 97452.0 26 125852.5 27 154253.0 28 154253.0 29 154253.0 30 176945.5 31 199638.0 32 199638.0 33 199638.0 34 210434.5 35 221231.0 36 221231.0 37 221231.0 38 216157.0 39 211083.0 40 211083.0 41 211083.0 42 212928.0 43 214773.0 44 214773.0 45 214773.0 46 223327.0 47 231881.0 48 231881.0 49 231881.0 50 220026.0 51 208171.0 52 208171.0 53 208171.0 54 190071.0 55 171971.0 56 171971.0 57 171971.0 58 152448.5 59 132926.0 60 132926.0 61 132926.0 62 114339.0 63 95752.0 64 95752.0 65 95752.0 66 73438.0 67 51124.0 68 51124.0 69 51124.0 70 37696.5 71 24269.0 72 24269.0 73 24269.0 74 17579.5 75 10890.0 76 10890.0 77 10890.0 78 7399.5 79 3909.0 80 3909.0 81 3909.0 82 2606.0 83 1303.0 84 1303.0 85 1303.0 86 823.0 87 343.0 88 343.0 89 343.0 90 221.5 91 100.0 92 100.0 93 100.0 94 63.0 95 26.0 96 26.0 97 26.0 98 17.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09320527901224863 2 0.0 3 0.1250223421288797 4 0.010480914909007879 5 0.02208478498683803 6 0.01253966605184871 7 0.02110219921411854 8 0.018949868473875853 9 1.403693961027841E-4 10 0.37689182853597525 11 0.06424239361637418 12 0.001544063357130625 13 0.0 14 9.825857727194887E-4 15 0.0014036939610278408 16 1.403693961027841E-4 17 9.357959740185606E-5 18 7.018469805139204E-4 19 5.614775844111364E-4 20 3.275285909064962E-4 21 3.275285909064962E-4 22 0.0028541777207566096 23 1.8715919480371212E-4 24 0.0 25 0.0022927001363454735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2137218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.06120507510614 #Duplication Level Percentage of deduplicated Percentage of total 1 84.36969853387994 56.57933655505405 2 10.964340709109303 14.705638016138273 3 2.5396522391463883 5.109364188865453 4 0.7972527536268111 2.138589216306426 5 0.39922919260874784 1.3386395378752147 6 0.20067583989729607 0.8074538191783044 7 0.13363405208539575 0.6273162400341299 8 0.08825128201441497 0.4734589857047767 9 0.06679693184927996 0.4031534470619207 >10 0.36581135087591377 4.521123593414725 >50 0.03833168025751333 1.7974898205236904 >100 0.03134871505602144 4.138470328357971 >500 0.0028738803283118067 1.3857460695503994 >1k 0.0016822714116946883 1.9070460665420292 >5k 2.1028392646183603E-4 0.9757100124035717 >10k+ 2.1028392646183603E-4 3.091464102989146 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 28278 1.323121927664843 No Hit GGTATCAACGCAGAGTACTTTTTTT 19481 0.9115120684927789 No Hit TATCAACGCAGAGTACTTTTTTTTT 18008 0.8425906950063119 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9611 0.4496967553146193 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6090 0.2849498740886517 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5056 0.2365692222318921 No Hit CCCATGTACTCTGCGTTGATACCAC 3512 0.16432577303765922 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3415 0.15978716256366923 No Hit GTACATGGGAAGCAGTGGTATCAAC 2995 0.14013544710927944 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2825 0.13218118133012166 No Hit GTACTGGTTCACTATCGGTCAGTCA 2597 0.12151310722631008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.678979870092803E-5 0.0 10 0.0 0.0 0.0 9.357959740185606E-5 0.0 11 0.0 0.0 0.0 9.357959740185606E-5 0.0 12 0.0 0.0 0.0 9.357959740185606E-5 0.0 13 0.0 0.0 0.0 9.357959740185606E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 65 5.540027E-5 13.132011 19 GGTATCA 4300 0.0 12.432903 1 CATACCG 55 0.0030283553 12.112497 5 AAATCGC 95 1.3860288E-5 10.981742 17 GTACTAG 140 8.183633E-9 10.871679 1 AATCGCC 90 9.679635E-5 10.5380335 18 CTACACT 380 0.0 10.518748 4 TGTCCCG 390 0.0 10.462848 11 CTAGGGC 130 3.8266808E-7 10.2339 3 ACACCGT 95 1.6151342E-4 10.017856 6 ACCGTGT 105 4.026448E-5 9.970151 8 CGCAAAA 115 1.02298745E-5 9.916078 2 GTCCAAG 195 1.6007107E-10 9.756635 1 GTATCAA 8360 0.0 9.740296 1 CCCCGGT 205 4.1836756E-11 9.715553 19 ACTGAGC 380 0.0 9.516963 8 GGACTAG 100 2.7191307E-4 9.512719 1 GTATGGG 110 6.7385285E-5 9.512719 1 ATAGGAC 110 6.815045E-5 9.502907 3 TGACGAA 90 0.0011306833 9.484452 12 >>END_MODULE