FastQCFastQC Report
Wed 25 May 2016
SRR1294632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369242
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102180.43127717641338453No Hit
GGTATCAACGCAGAGTACTTTTTTT63930.26983313650526203No Hit
TATCAACGCAGAGTACTTTTTTTTT60060.253498798349852No Hit
GTACTGGTTCACTATCGGTCAGTCA31170.13156106467807002No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26620.11235661025762671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.013.4073371
ACCGTCT500.001504443713.29472358
TACCGTC550.003077910512.0863687
CGAACAC951.3506869E-511.00749118
AGCGTCG1701.382432E-1010.623754517
GTATCAA43250.010.3883251
TAGACAG2700.010.1998715
TGTCCCG3950.010.09747111
AGCGTAC3000.09.8154671
AGAGGCG3000.09.49763519
ATAACGG800.0045309439.4964315
ACGATGC800.00453173239.496238
GTGTATG3650.09.3687171
GCGTACA3400.09.2175132
TAGGGGT1451.7156071E-69.1689684
CTAATAC2201.6916601E-109.0649673
TCTTCGC950.00181467169.00517713
CGCCCTA4350.08.95917216
CGCAATT850.0074021638.9463212
CCGGCGA2356.548362E-118.89967318
Produced by FastQC (version 0.11.5)