##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294632_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2369242 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.849463668126766 31.0 30.0 33.0 25.0 34.0 2 29.921260048572496 31.0 30.0 34.0 25.0 34.0 3 29.963573159685673 31.0 30.0 34.0 25.0 34.0 4 33.50751886046255 35.0 33.0 37.0 27.0 37.0 5 34.04246337014117 35.0 35.0 37.0 28.0 37.0 6 34.152730282512294 37.0 35.0 37.0 30.0 37.0 7 34.01082709153392 37.0 35.0 37.0 29.0 37.0 8 33.819322804508786 36.0 35.0 37.0 28.0 37.0 9 34.88144098407845 38.0 35.0 39.0 27.0 39.0 10 35.02977872247748 38.0 35.0 39.0 27.0 39.0 11 35.27968649888867 39.0 35.0 39.0 27.0 39.0 12 35.06849405843725 38.0 35.0 39.0 27.0 39.0 13 34.90363120356637 38.0 35.0 39.0 27.0 39.0 14 35.76482562777462 39.0 35.0 41.0 27.0 41.0 15 35.77521713695773 39.0 34.0 41.0 27.0 41.0 16 35.906966025420786 39.0 35.0 41.0 27.0 41.0 17 35.91094029229602 39.0 35.0 41.0 27.0 41.0 18 35.79944682729751 39.0 34.0 41.0 27.0 41.0 19 36.091105509694664 39.0 35.0 41.0 27.0 41.0 20 36.19454407781054 39.0 35.0 41.0 27.0 41.0 21 36.24846174430472 39.0 35.0 41.0 27.0 41.0 22 36.2744012642018 39.0 35.0 41.0 27.0 41.0 23 36.25625959695126 39.0 35.0 41.0 27.0 41.0 24 36.26951700164019 39.0 35.0 41.0 27.0 41.0 25 35.1029679534636 38.0 33.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0766391806865734 1101 2 1.1634840047712842 1101 3 1.4444683128325657 1101 4 1.275257096454503 1101 5 0.8699044387191321 1101 6 0.8369898209664228 1101 7 0.9060268642413263 1101 8 1.0791228202524579 1101 9 1.3196622511129945 1101 10 1.2438338637242552 1101 11 0.9417996769185208 1101 12 1.2496509747562001 1101 13 1.185237066156489 1101 14 1.3978944490122274 1101 15 1.6285733415403314 1101 16 1.4850650839802597 1101 17 1.465372407523617 1101 18 1.5144658182915904 1101 19 1.3240680449540463 1101 20 1.2506432726952639 1101 21 1.2732730826443301 1101 22 1.1257355388383985 1101 23 1.1267418769730995 1101 24 1.2053645094086036 1101 25 1.2186997636693917 1102 1 1.037589563071787 1102 2 0.923577571550716 1102 3 1.2046695535646705 1102 4 1.0871815815979673 1102 5 0.8645485640756618 1102 6 0.7752232924414173 1102 7 0.7626970872568606 1102 8 0.8225008849025883 1102 9 1.2529638682758844 1102 10 1.173880955409004 1102 11 0.8193851957961371 1102 12 1.117414315663396 1102 13 1.13296841979745 1102 14 1.436212158921272 1102 15 1.3425386328187514 1102 16 1.3017240126962832 1102 17 1.3858489001799015 1102 18 1.4913249380078497 1102 19 1.101779832306498 1102 20 0.2364882500264045 1102 21 0.6658571041253083 1102 22 0.8104040877864023 1102 23 0.9439576221968622 1102 24 1.1190540269543874 1102 25 1.0797399796614044 1103 1 0.7095293949051431 1103 2 0.7901460814200227 1103 3 1.0916137244485036 1103 4 0.9063845614043231 1103 5 0.6796608105987332 1103 6 0.5171996243023926 1103 7 0.41549894378542973 1103 8 0.684738144832842 1103 9 0.781607126821541 1103 10 0.8400308362882569 1103 11 0.67787364612132 1103 12 0.8746998576871832 1103 13 0.6454164568240088 1103 14 0.7588990549888308 1103 15 1.0096135277552278 1103 16 1.0479932418404019 1103 17 1.0136597760830526 1103 18 1.0768094328091138 1103 19 0.8342076529330811 1103 20 -0.07709996595755086 1103 21 0.4985400674205209 1103 22 -0.12229155138660985 1103 23 0.4647769389345413 1103 24 0.5264573896372511 1103 25 0.48515030432638184 1104 1 0.37300509876404675 1104 2 0.3203927936858939 1104 3 0.708431443698057 1104 4 0.5636079570212331 1104 5 0.3783516064155421 1104 6 0.18925132597826888 1104 7 0.2238509455051485 1104 8 0.34258474431540265 1104 9 0.6660005030351073 1104 10 0.5850232548252592 1104 11 0.36848811639681145 1104 12 0.5610533469883521 1104 13 0.3877109100985692 1104 14 0.6957074198001436 1104 15 0.7284052739603837 1104 16 0.6035363477151208 1104 17 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-0.24627355027156028 2207 11 0.20015364029013938 2207 12 0.449933228416171 2207 13 0.588186031267071 2207 14 0.8923463359161303 2207 15 0.7653236895847542 2207 16 0.9303994657171373 2207 17 0.7318344173206768 2207 18 0.8486180374722352 2207 19 -0.37757416207321626 2207 20 0.33682910624282414 2207 21 -0.3727170393387951 2207 22 0.18819550063138024 2207 23 -0.5399689994816441 2207 24 -1.625789554095313 2207 25 -0.17437879210018536 2208 1 0.5970234953347635 2208 2 0.2900059558995842 2208 3 0.785460624105589 2208 4 0.6350978934153275 2208 5 0.5153107562300292 2208 6 0.45550422218104103 2208 7 0.4693535394792576 2208 8 0.6280346886397368 2208 9 0.08132355852345086 2208 10 0.25090219740007313 2208 11 0.4239943425806203 2208 12 0.3753838303703674 2208 13 0.7387900639477394 2208 14 0.9080570321574015 2208 15 0.8464297295140142 2208 16 0.6049430215402012 2208 17 0.6645664511137852 2208 18 0.8262138478725731 2208 19 -1.2162152973622824 2208 20 0.007207096757596787 2208 21 -0.03491639334971097 2208 22 0.44552117045496686 2208 23 0.5711664110125056 2208 24 0.6766199695574144 2208 25 0.7408540993372768 2209 1 0.127575304696407 2209 2 -1.5789144459966096 2209 3 -0.38477129399436194 2209 4 0.046473317466670494 2209 5 0.0011607422661370492 2209 6 0.08065396529522673 2209 7 0.11032294052765934 2209 8 0.32117294547856545 2209 9 -2.3571446690769733 2209 10 -0.5419539168715204 2209 11 -0.18587948521114583 2209 12 0.12907872827773303 2209 13 0.25047723120921006 2209 14 0.495494177082179 2209 15 0.5509600197956956 2209 16 0.5105087554205454 2209 17 0.31298581551894245 2209 18 0.4187503143620219 2209 19 0.22157493334437817 2209 20 0.18952688049667898 2209 21 0.2659327245455998 2209 22 0.22467611168721646 2209 23 0.3035591896645258 2209 24 0.26645304687540516 2209 25 0.45581931858188085 2210 1 0.2529077389851544 2210 2 0.34164568234951886 2210 3 0.23900544645493582 2210 4 0.3663960447211565 2210 5 0.21880634534632293 2210 6 0.22302371292312984 2210 7 0.23688490026723485 2210 8 0.37530443023686644 2210 9 0.5933860174376733 2210 10 -1.2198217072755995 2210 11 -0.19477100029853744 2210 12 -2.0348813684207983 2210 13 -0.5268912171250619 2210 14 -0.20778473109102436 2210 15 -0.4949021420154196 2210 16 0.126936822588263 2210 17 0.2781516806200699 2210 18 0.5571979287470441 2210 19 0.37562290977246704 2210 20 0.5694931560772432 2210 21 0.5694471622469237 2210 22 0.3950397661702425 2210 23 0.35551492333247126 2210 24 0.37444108534679543 2210 25 0.3525560890357653 2211 1 -0.15852746441843024 2211 2 0.11539987288195874 2211 3 -1.3438212306556387 2211 4 -0.42790582008081657 2211 5 -0.3229557643558465 2211 6 -0.20027532224211342 2211 7 -0.3209491023960638 2211 8 0.06728411486881214 2211 9 0.47333185690235524 2211 10 0.325117941662711 2211 11 0.03417348132182951 2211 12 0.023869430841777728 2211 13 -0.014557338343557547 2211 14 -0.5790141650861713 2211 15 -0.10076695859507367 2211 16 0.07908517177974517 2211 17 0.11381777226016965 2211 18 0.09936406863022285 2211 19 0.19731427900179455 2211 20 0.23106326636100505 2211 21 0.03592735732890873 2211 22 -0.02909677411441436 2211 23 -0.3341066415980336 2211 24 -0.07679884185115071 2211 25 0.1415229736173913 2212 1 -0.3517821196672415 2212 2 -0.3421843498234516 2212 3 -2.616786704966419 2212 4 -1.4844958276644746 2212 5 -0.6603213757602617 2212 6 -0.4806882500347527 2212 7 -0.3316179892010709 2212 8 -0.406773242330722 2212 9 -1.0014511863092324 2212 10 -0.30115185380747533 2212 11 -0.30340433515665666 2212 12 -0.01873770314168155 2212 13 -0.4168690569107767 2212 14 -0.20005406918168944 2212 15 -0.08722853249263096 2212 16 -1.2861936126363105 2212 17 -1.470977285727919 2212 18 -0.33474053091475753 2212 19 -0.16739763925192364 2212 20 -0.2220496217767689 2212 21 -0.09921125533080044 2212 22 -0.38896485392947966 2212 23 -0.13985608236514224 2212 24 -0.15012828984697535 2212 25 0.04578451713450704 2213 1 -3.268976347403097 2213 2 -1.3710557362373628 2213 3 -2.5333262675071744 2213 4 -1.0241560649149832 2213 5 -0.8043619290816295 2213 6 -0.4904533600311396 2213 7 -0.317571601721383 2213 8 -0.20155731531533405 2213 9 -0.27919916172209014 2213 10 -0.267220381089075 2213 11 -0.5016938767765495 2213 12 -0.34727621095503736 2213 13 -0.6182714654155461 2213 14 -0.23887687971443228 2213 15 -0.40874848468253333 2213 16 -1.7449139819803747 2213 17 -1.037644781334535 2213 18 -0.3189835666349694 2213 19 -0.23948067850894006 2213 20 -0.14413281892711183 2213 21 -0.07118538766751215 2213 22 0.04815235344288027 2213 23 0.043157674880184516 2213 24 0.14224317314519652 2213 25 0.06683959190489475 2214 1 -1.0202696678593846 2214 2 -0.6463421884998901 2214 3 -6.625419225159845 2214 4 -2.3130295322558005 2214 5 -1.3148538492569983 2214 6 -0.8003129205529333 2214 7 -0.5269964639559106 2214 8 -0.35665341321745814 2214 9 -0.32377307316431114 2214 10 -0.3952006306243021 2214 11 -0.9493608261897322 2214 12 -0.6730145026044809 2214 13 -5.333185691352867 2214 14 -4.04672143050988 2214 15 -2.364520529605926 2214 16 -2.8982764780379213 2214 17 -3.310816413696152 2214 18 -1.2290952482454074 2214 19 -0.6626857115275939 2214 20 -0.4496352098656402 2214 21 -0.3516422812300988 2214 22 -0.40144184326642574 2214 23 -0.34595077244961914 2214 24 -0.09928755643877452 2214 25 0.16459479349401818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 921.0 3 86.0 4 124.0 5 257.0 6 39.0 7 51.0 8 67.0 9 208.0 10 441.0 11 1127.0 12 2896.0 13 5340.0 14 8685.0 15 12264.0 16 15413.0 17 17360.0 18 18258.0 19 18165.0 20 17733.0 21 17894.0 22 17755.0 23 18312.0 24 19428.0 25 21285.0 26 23447.0 27 27139.0 28 31878.0 29 38811.0 30 48496.0 31 61032.0 32 80572.0 33 108134.0 34 144513.0 35 197984.0 36 273349.0 37 407069.0 38 712709.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26701243743889 20.99644849815327 13.507663655959773 23.22887540844806 2 15.654726084523695 21.737518343732514 39.815496362926126 22.79225920881766 3 18.099517510056273 26.558569211440396 30.156318295554126 25.185594982949205 4 12.354948056000955 18.39705645745347 37.932611524089 31.315383962456572 5 12.627729088914394 39.10167569078645 34.856116596525986 13.41447862377317 6 29.427680247672267 39.849470676558354 17.499709627076694 13.223139448692688 7 25.79548564535911 34.56774741778644 22.159480617771578 17.47728631908287 8 23.835454681367022 37.37994301965425 21.013424082938243 17.771178216040482 9 24.535865201663228 17.824077104851362 21.51510863987397 36.12494905361144 10 14.751901432959071 30.888402526008495 33.327997607792916 21.031698433239516 11 31.416156250488314 26.598767906094967 23.569170032253037 18.415905811163682 12 20.527411637011728 28.385329780090295 30.840277220530538 20.24698136236744 13 26.520700378029122 24.684444298036116 26.594228330265096 22.20062699366967 14 20.280494376935383 24.77062592809708 28.0045004789361 26.944379216031432 15 22.32278010541965 31.671594134342477 24.492836869847277 21.512788890390595 16 20.97116081063103 31.532508230265837 26.021957230069454 21.474373729033676 17 19.7331210352488 30.59385743367093 27.899938337824253 21.773083193256014 18 20.16031745629185 29.763260385678286 29.23223628649179 20.84418587153807 19 20.92574172743457 29.53339119747087 29.43244573125276 20.108421343841798 20 22.044430366381587 28.55909618836448 29.092007179812057 20.304466265441874 21 22.373252290931635 28.4354260934347 28.49429896098258 20.697022654651086 22 21.91696523865979 28.401387193003725 28.728250140750845 20.953397427585646 23 21.908909375649362 28.409542055016917 28.65632079631441 21.02522777301931 24 21.7505315918017 28.156413442830967 28.732610802148162 21.36044416321917 25 21.64497044919005 28.55204198989955 28.810403033936655 20.992584526973744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2518.0 1 2518.0 2 2678.0 3 2838.0 4 2838.0 5 2838.0 6 4706.0 7 6574.0 8 6574.0 9 6574.0 10 9590.5 11 12607.0 12 12607.0 13 12607.0 14 19730.5 15 26854.0 16 26854.0 17 26854.0 18 41101.0 19 55348.0 20 55348.0 21 55348.0 22 77943.0 23 100538.0 24 100538.0 25 100538.0 26 128711.0 27 156884.0 28 156884.0 29 156884.0 30 183209.5 31 209535.0 32 209535.0 33 209535.0 34 226639.5 35 243744.0 36 243744.0 37 243744.0 38 247975.0 39 252206.0 40 252206.0 41 252206.0 42 254849.5 43 257493.0 44 257493.0 45 257493.0 46 259274.5 47 261056.0 48 261056.0 49 261056.0 50 249846.0 51 238636.0 52 238636.0 53 238636.0 54 217461.5 55 196287.0 56 196287.0 57 196287.0 58 171235.5 59 146184.0 60 146184.0 61 146184.0 62 123865.5 63 101547.0 64 101547.0 65 101547.0 66 77733.5 67 53920.0 68 53920.0 69 53920.0 70 40348.0 71 26776.0 72 26776.0 73 26776.0 74 19145.5 75 11515.0 76 11515.0 77 11515.0 78 7828.5 79 4142.0 80 4142.0 81 4142.0 82 2794.0 83 1446.0 84 1446.0 85 1446.0 86 936.5 87 427.0 88 427.0 89 427.0 90 277.0 91 127.0 92 127.0 93 127.0 94 74.0 95 21.0 96 21.0 97 21.0 98 20.0 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0994410870649769 2 0.05347701923231143 3 0.05558739883895356 4 0.051113394072872256 5 0.04828548539997181 6 0.06740552463614945 7 0.06356463375206078 8 0.059428289723042226 9 0.06571722095083575 10 0.06559059817443723 11 0.059217251762378006 12 0.06128542377688729 13 0.06056789471062897 14 0.06305814264646667 15 0.06592825891149998 16 0.0614964617375515 17 0.06339580338352942 18 0.1450252865684468 19 0.06867175240013473 20 0.06297372746220099 21 0.060272441565699074 22 0.06681461834628966 23 0.06474644633178038 24 0.0769866480503047 25 0.06858733721586904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2369242.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.53387639290136 #Duplication Level Percentage of deduplicated Percentage of total 1 85.65638929807784 62.98646342907096 2 10.162030467371832 14.945069845772357 3 2.273412224884183 5.015184406042331 4 0.7518851271854878 2.211561120164743 5 0.35244643955388316 1.2958376460636714 6 0.18914937717736033 0.8345332152692576 7 0.12768788246768006 0.6572569476374791 8 0.08393236502355284 0.49374977240046497 9 0.053998372724131466 0.35736386987826896 >10 0.302097403852519 3.9411852630473874 >50 0.02502261635194909 1.2821157618607235 >100 0.0186519282095503 2.708880419149025 >500 0.0020819981811385857 1.1250957590108157 >1k 0.0010409990905668358 1.1838681485973135 >5k 1.1566656561853732E-4 0.5272929511623712 >10k+ 5.783328280926866E-5 0.4345414448727405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10218 0.43127717641338453 No Hit GGTATCAACGCAGAGTACTTTTTTT 6393 0.26983313650526203 No Hit TATCAACGCAGAGTACTTTTTTTTT 6006 0.253498798349852 No Hit GTACTGGTTCACTATCGGTCAGTCA 3117 0.13156106467807002 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2662 0.11235661025762671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.2207592132842485E-5 0.0 0.0 4.2207592132842485E-5 6 0.0 4.2207592132842485E-5 0.0 0.0 4.2207592132842485E-5 7 0.0 8.441518426568497E-5 0.0 0.0 4.2207592132842485E-5 8 0.0 8.441518426568497E-5 0.0 8.441518426568497E-5 4.2207592132842485E-5 9 0.0 8.441518426568497E-5 0.0 1.2662277639852745E-4 4.2207592132842485E-5 10 0.0 8.441518426568497E-5 0.0 1.2662277639852745E-4 4.2207592132842485E-5 11 0.0 1.2662277639852745E-4 0.0 1.2662277639852745E-4 4.2207592132842485E-5 12 0.0 1.2662277639852745E-4 0.0 1.2662277639852745E-4 4.2207592132842485E-5 13 0.0 1.2662277639852745E-4 0.0 1.6883036853136994E-4 4.2207592132842485E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2005 0.0 13.407337 1 ACCGTCT 50 0.0015044437 13.2947235 8 TACCGTC 55 0.0030779105 12.086368 7 CGAACAC 95 1.3506869E-5 11.007491 18 AGCGTCG 170 1.382432E-10 10.6237545 17 GTATCAA 4325 0.0 10.388325 1 TAGACAG 270 0.0 10.199871 5 TGTCCCG 395 0.0 10.097471 11 AGCGTAC 300 0.0 9.815467 1 AGAGGCG 300 0.0 9.497635 19 ATAACGG 80 0.004530943 9.496431 5 ACGATGC 80 0.0045317323 9.49623 8 GTGTATG 365 0.0 9.368717 1 GCGTACA 340 0.0 9.217513 2 TAGGGGT 145 1.7156071E-6 9.168968 4 CTAATAC 220 1.6916601E-10 9.064967 3 TCTTCGC 95 0.0018146716 9.005177 13 CGCCCTA 435 0.0 8.959172 16 CGCAATT 85 0.007402163 8.94632 12 CCGGCGA 235 6.548362E-11 8.899673 18 >>END_MODULE