FastQCFastQC Report
Wed 25 May 2016
SRR1294632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369242
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT262501.1079492934871153No Hit
GGTATCAACGCAGAGTACTTTTTTT178540.7535743499397698No Hit
TATCAACGCAGAGTACTTTTTTTTT169620.7159251777572743No Hit
ACGCAGAGTACTTTTTTTTTTTTTT89520.37784236477320593No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56710.23935925498534974No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46880.1978691919187656No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32880.1387785629327861No Hit
GTACTGGTTCACTATCGGTCAGTCA31220.13177210263873423No Hit
ATCAACGCAGAGTACTTTTTTTTTT25200.10636313217476306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC1001.8189894E-1216.11964618
GGACCGT601.4321031E-615.8705656
GACGAAC508.8611836E-515.17079313
GGTATCA31400.015.0155591
AAATCGC1250.014.41286117
ATCGCCG1006.7848305E-1014.22321719
GGCGATC550.003112807412.06818518
TTTCGTC700.001515497710.83673717
GTATCAA70700.010.8234581
CTAGTAC1606.206392E-910.0907523
GAAATCG1651.0546501E-89.76948316
AGGACTG3400.09.5223395
CGAGACT800.004430249.5223394
GTTCTAG1606.5760105E-89.5058181
CCCCCGG3200.09.48214518
TAGACTG1856.4355845E-99.2649785
GTCTAAG1951.6643753E-99.2620791
CTAGACT1451.6532867E-69.1939824
GCGCCTT3750.09.10247513
GTCCTAC1151.1035548E-49.0925221
Produced by FastQC (version 0.11.5)